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The map label for this gene is ltaA [H]

Identifier: 116624995

GI number: 116624995

Start: 7427133

End: 7428209

Strand: Reverse

Name: ltaA [H]

Synonym: Acid_5925

Alternate gene names: 116624995

Gene position: 7428209-7427133 (Counterclockwise)

Preceding gene: 116624996

Following gene: 116624994

Centisome position: 74.54

GC content: 64.44

Gene sequence:

>1077_bases
ATGATCGATCTGCGCAGCGATACCGTCACCAGGCCCACGCCCGCCATGCGCCAGGCCATGTTCGAAGCCGAGGTCGGCGA
CGACGTTTATCGCGAAGACCCCACCGTCAATCGCCTCGAAGCCCGTGCCGCTGAAATCGCCGGCAAAGAGGCAGCCCTCT
TCGTCCCCACCGGGACCATGGGGAACACCATCGCCATCAAGCTGCTCACCGAGCACGGCCAGGAAGTGATCTGCGATTCG
CGCGCCCATCTGCTGGATTATGAGCTCTCCATGATCGCCTGGTTCAGCGGCTGCCTCATCCGCGCCATTCCCACCGAGCG
CGGCCTGCTTTCCTGGGACGAAGTGCGGTGCCAGATACGCCCCGTGAACCCGTACGGCGCGCCCACCGGCGCGGTCGCTG
TCGAACAGACCCACAATATGGCCGGCGGCACCGTGTACCCCATGAGCACACTGTGCGAGATTTGCGACGGCGCGCACGAG
CGCGGGGTCCGCGTACATATGGATGGAGCGCGTATATTCAATGCCTCCGCCGCTCTGGGCATTCCCGTGCATGAGATTGC
TTCTCGCGCGGATACATTAATGTTCTGTCTCTCCAAAGGACTGGGTGCGCCCTCCGGCTCCATACTCGCCGGACCCGCGC
AGCTCATCGATAAGGGCCGCCTCTATCGCAAGCGTCTCGGCGGCGGCATGCGCCAGTCCGGGATACTCGCCGCGGCCGGT
CTCATCGCCCTGGAGCAATCGCCCGCGAAGTTGCCCGCCGACCACGCCAACGCGCGATTCCTGGCCGAGGGCCTGGCGCG
CATCCCTGGAATTCAAATCGACCCCGCGAAGGTCGAAACCAATATCGTCGTCTTCGATGTCTCGGCCACCGGCCACGCGC
CCGCCGATATCAGCGCCGGTCTGCGCCGCCGCGGCGTCCTCATGAACGCCATCAACGAACGCTGCGTCCGTGCCGTAACC
CATTGCGATGTGGACCGCGCCCAGTGCGCGCAGGCACTCGACGCCATCGCCGAAATCTTATCGGAAACCCCAATCGGAAC
CCAGGCTGCCACCACTGCCTCCGCTAAAACGTCATAA

Upstream 100 bases:

>100_bases
TCCTCGATGCCGGATTGCTCGAAACCAGCGACGGCATTCTGCGTCTCACCAACCGCGGCGTCCTGCTCTCCAACGAAGTC
TTCCAGGAGTTCCTAAACAT

Downstream 100 bases:

>100_bases
TGGACTTATCAACTGCATGCTGAGATTCTCTTTCCTGCTCATGGCAGGCGCTTCTGCCTTTGCGCAGCAATATACAATTT
CGACCGTTGCCGGCGGCGCG

Product: L-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MIDLRSDTVTRPTPAMRQAMFEAEVGDDVYREDPTVNRLEARAAEIAGKEAALFVPTGTMGNTIAIKLLTEHGQEVICDS
RAHLLDYELSMIAWFSGCLIRAIPTERGLLSWDEVRCQIRPVNPYGAPTGAVAVEQTHNMAGGTVYPMSTLCEICDGAHE
RGVRVHMDGARIFNASAALGIPVHEIASRADTLMFCLSKGLGAPSGSILAGPAQLIDKGRLYRKRLGGGMRQSGILAAAG
LIALEQSPAKLPADHANARFLAEGLARIPGIQIDPAKVETNIVVFDVSATGHAPADISAGLRRRGVLMNAINERCVRAVT
HCDVDRAQCAQALDAIAEILSETPIGTQAATTASAKTS

Sequences:

>Translated_358_residues
MIDLRSDTVTRPTPAMRQAMFEAEVGDDVYREDPTVNRLEARAAEIAGKEAALFVPTGTMGNTIAIKLLTEHGQEVICDS
RAHLLDYELSMIAWFSGCLIRAIPTERGLLSWDEVRCQIRPVNPYGAPTGAVAVEQTHNMAGGTVYPMSTLCEICDGAHE
RGVRVHMDGARIFNASAALGIPVHEIASRADTLMFCLSKGLGAPSGSILAGPAQLIDKGRLYRKRLGGGMRQSGILAAAG
LIALEQSPAKLPADHANARFLAEGLARIPGIQIDPAKVETNIVVFDVSATGHAPADISAGLRRRGVLMNAINERCVRAVT
HCDVDRAQCAQALDAIAEILSETPIGTQAATTASAKTS
>Mature_358_residues
MIDLRSDTVTRPTPAMRQAMFEAEVGDDVYREDPTVNRLEARAAEIAGKEAALFVPTGTMGNTIAIKLLTEHGQEVICDS
RAHLLDYELSMIAWFSGCLIRAIPTERGLLSWDEVRCQIRPVNPYGAPTGAVAVEQTHNMAGGTVYPMSTLCEICDGAHE
RGVRVHMDGARIFNASAALGIPVHEIASRADTLMFCLSKGLGAPSGSILAGPAQLIDKGRLYRKRLGGGMRQSGILAAAG
LIALEQSPAKLPADHANARFLAEGLARIPGIQIDPAKVETNIVVFDVSATGHAPADISAGLRRRGVLMNAINERCVRAVT
HCDVDRAQCAQALDAIAEILSETPIGTQAATTASAKTS

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=334, Percent_Identity=44.6107784431138, Blast_Score=237, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI193206522, Length=262, Percent_Identity=47.7099236641221, Blast_Score=214, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI193206524, Length=262, Percent_Identity=47.7099236641221, Blast_Score=213, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI193206520, Length=262, Percent_Identity=47.7099236641221, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6320789, Length=342, Percent_Identity=35.3801169590643, Blast_Score=186, Evalue=4e-48,
Organism=Drosophila melanogaster, GI21355439, Length=299, Percent_Identity=43.1438127090301, Blast_Score=228, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 38013; Mature: 38013

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLRSDTVTRPTPAMRQAMFEAEVGDDVYREDPTVNRLEARAAEIAGKEAALFVPTGTM
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCC
GNTIAIKLLTEHGQEVICDSRAHLLDYELSMIAWFSGCLIRAIPTERGLLSWDEVRCQIR
CCEEEEEEEHHCCCEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHCEEEEE
PVNPYGAPTGAVAVEQTHNMAGGTVYPMSTLCEICDGAHERGVRVHMDGARIFNASAALG
ECCCCCCCCCCEEEEHHCCCCCCEECCHHHHHHHHCCHHHCCEEEEECCCEEEECCCCCC
IPVHEIASRADTLMFCLSKGLGAPSGSILAGPAQLIDKGRLYRKRLGGGMRQSGILAAAG
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LIALEQSPAKLPADHANARFLAEGLARIPGIQIDPAKVETNIVVFDVSATGHAPADISAG
HHEECCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCEEEEEEEEEEECCCCCCCCHHHHH
LRRRGVLMNAINERCVRAVTHCDVDRAQCAQALDAIAEILSETPIGTQAATTASAKTS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIDLRSDTVTRPTPAMRQAMFEAEVGDDVYREDPTVNRLEARAAEIAGKEAALFVPTGTM
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCC
GNTIAIKLLTEHGQEVICDSRAHLLDYELSMIAWFSGCLIRAIPTERGLLSWDEVRCQIR
CCEEEEEEEHHCCCEEECCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHCEEEEE
PVNPYGAPTGAVAVEQTHNMAGGTVYPMSTLCEICDGAHERGVRVHMDGARIFNASAALG
ECCCCCCCCCCEEEEHHCCCCCCEECCHHHHHHHHCCHHHCCEEEEECCCEEEECCCCCC
IPVHEIASRADTLMFCLSKGLGAPSGSILAGPAQLIDKGRLYRKRLGGGMRQSGILAAAG
CCHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LIALEQSPAKLPADHANARFLAEGLARIPGIQIDPAKVETNIVVFDVSATGHAPADISAG
HHEECCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCEEEEEEEEEEECCCCCCCCHHHHH
LRRRGVLMNAINERCVRAVTHCDVDRAQCAQALDAIAEILSETPIGTQAATTASAKTS
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]