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The map label for this gene is mdtE [H]

Identifier: 116624875

GI number: 116624875

Start: 7266215

End: 7267486

Strand: Reverse

Name: mdtE [H]

Synonym: Acid_5799

Alternate gene names: 116624875

Gene position: 7267486-7266215 (Counterclockwise)

Preceding gene: 116624878

Following gene: 116624874

Centisome position: 72.93

GC content: 65.02

Gene sequence:

>1272_bases
ATGAGCAAGCTGCATAACCCCAACCTGGCGGTCGCACTCGCGATAGCCGGCCTTGCCGCCGGGTGTTCACGGCCAACCGA
GACTCGTTCCGAGGCCGCCCGTCCAGTCAAGACGATGGTAGTGACCGACGGCGACGAGCCTCTGGTACGTACTTTCCCGG
GAAGGGTTGAAGCGTCCAGGAGCGTTGACCTGGCGTTTCAAGTGCCTGGCCTGCTGGTCAACCTGCCGGTGAAGGAAGGG
CAGAAGGTCGCGAAAGGCGCGGTGATCGCGCAGCCCCGGCAGGACGAGTTTCAAGAGCGAGTGAAATCCTTGCAAGGCCA
GGTCGATCAGGCTCGGGCTTCGCTGAGCGCACTTCAGGTTGGCGAACGTCCCGAAGAGCGCCTAAGCCGCGAGGCGCAAT
TACGGGCGGTAGGCGCGAAGGTTGCGAACGCCAAGACGGAATTCGATCGATACTCTAGGCTGGTGCAATCCAGCGCTGTC
TCACGCGCGGAGTACGAACTTGCCGCGACCGCATACCGGGTCGCGCAGGAAGAGCAAACGGCAGCCCGGCAGTTGGTCGA
AAAGGGAACCGGCGCTCGGACGGAAGACATCGATGCCCAGGCGGCCGAGGTTCGCACGCTCGAAGCTCGCCTGGCGGAGG
CGAGTCTTCAGCTCGCGGACAGTACGCTGCGCGCACCGTACAGCGGCGTGGTTGCCCAGCGATTCGTTGACGAGAAGCAG
AGCATCGTGGCCAACAAGCCGGTGGTGACGCTGCAGAACGTTGACGAAATCGATATCGTCGCGGACGTGCCGGAGGCGGC
GATGGCGGCTGACATTCGCTCGACGGCGTTCACGAAGCTGGTGGCGGAGTTCAGCACCGCGCCCGGCGTGCAGTTTCCGG
TGCGCATAAAGGAAGTCGCGCAAGTCGCGGATCCGGCCACGCAGACTTTTAAAGTTCGCGTCGCGATGCCGGCGCCTCGC
CGTGTCAATGTGCTGCCCGGTATGACGGCCACGGTCGCGGCCACGTATCGCAGGGCTGGCGCGCGCGGCAGGCGCATCCT
GGTGCCGATCTCGGCGGTATTCAAGGAAGACACTGGGGAGCAGGTGGCGTGGATCATCGGGCCGGATCAGTTGATCGGCC
GGCGCCTGGTGAAGATGGGAGCGGCGACAGGCGGCCAGGTCGAAATCCTTGACGGGTTGCGGCCGGGCGATCGCATCGTG
GTGGCGGGCGCGCCGTTTCTGAGCCCGGGAATGCGCGTGCGGGACTTGGGCGACGCCCTGGGAGGCGATTGA

Upstream 100 bases:

>100_bases
GCATCACCAATCCGCCGGGCGAATCAATGCCAAAAGTGAATCCACGCAGTTGGTTGCAGCACATCGCGCATCGATTACAT
AGGTGTCACGGCGTCACAGA

Downstream 100 bases:

>100_bases
CGATGAACCTCGGCGAACTCTCGGTTCAGAAGAACCGGGTGGCTTTCGCACTGATGTTTCTCGTGTTCGCCGGAGGGCTG
GTGGCGTATCACCGGCTGGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSKLHNPNLAVALAIAGLAAGCSRPTETRSEAARPVKTMVVTDGDEPLVRTFPGRVEASRSVDLAFQVPGLLVNLPVKEG
QKVAKGAVIAQPRQDEFQERVKSLQGQVDQARASLSALQVGERPEERLSREAQLRAVGAKVANAKTEFDRYSRLVQSSAV
SRAEYELAATAYRVAQEEQTAARQLVEKGTGARTEDIDAQAAEVRTLEARLAEASLQLADSTLRAPYSGVVAQRFVDEKQ
SIVANKPVVTLQNVDEIDIVADVPEAAMAADIRSTAFTKLVAEFSTAPGVQFPVRIKEVAQVADPATQTFKVRVAMPAPR
RVNVLPGMTATVAATYRRAGARGRRILVPISAVFKEDTGEQVAWIIGPDQLIGRRLVKMGAATGGQVEILDGLRPGDRIV
VAGAPFLSPGMRVRDLGDALGGD

Sequences:

>Translated_423_residues
MSKLHNPNLAVALAIAGLAAGCSRPTETRSEAARPVKTMVVTDGDEPLVRTFPGRVEASRSVDLAFQVPGLLVNLPVKEG
QKVAKGAVIAQPRQDEFQERVKSLQGQVDQARASLSALQVGERPEERLSREAQLRAVGAKVANAKTEFDRYSRLVQSSAV
SRAEYELAATAYRVAQEEQTAARQLVEKGTGARTEDIDAQAAEVRTLEARLAEASLQLADSTLRAPYSGVVAQRFVDEKQ
SIVANKPVVTLQNVDEIDIVADVPEAAMAADIRSTAFTKLVAEFSTAPGVQFPVRIKEVAQVADPATQTFKVRVAMPAPR
RVNVLPGMTATVAATYRRAGARGRRILVPISAVFKEDTGEQVAWIIGPDQLIGRRLVKMGAATGGQVEILDGLRPGDRIV
VAGAPFLSPGMRVRDLGDALGGD
>Mature_422_residues
SKLHNPNLAVALAIAGLAAGCSRPTETRSEAARPVKTMVVTDGDEPLVRTFPGRVEASRSVDLAFQVPGLLVNLPVKEGQ
KVAKGAVIAQPRQDEFQERVKSLQGQVDQARASLSALQVGERPEERLSREAQLRAVGAKVANAKTEFDRYSRLVQSSAVS
RAEYELAATAYRVAQEEQTAARQLVEKGTGARTEDIDAQAAEVRTLEARLAEASLQLADSTLRAPYSGVVAQRFVDEKQS
IVANKPVVTLQNVDEIDIVADVPEAAMAADIRSTAFTKLVAEFSTAPGVQFPVRIKEVAQVADPATQTFKVRVAMPAPRR
VNVLPGMTATVAATYRRAGARGRRILVPISAVFKEDTGEQVAWIIGPDQLIGRRLVKMGAATGGQVEILDGLRPGDRIVV
AGAPFLSPGMRVRDLGDALGGD

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=419, Percent_Identity=23.3890214797136, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786668, Length=435, Percent_Identity=24.367816091954, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789665, Length=423, Percent_Identity=23.1678486997636, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082036, Length=399, Percent_Identity=23.8095238095238, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45227; Mature: 45096

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLHNPNLAVALAIAGLAAGCSRPTETRSEAARPVKTMVVTDGDEPLVRTFPGRVEASR
CCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCCCC
SVDLAFQVPGLLVNLPVKEGQKVAKGAVIAQPRQDEFQERVKSLQGQVDQARASLSALQV
CEEEEEECCCEEEECCHHHCCHHHCCCEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHC
GERPEERLSREAQLRAVGAKVANAKTEFDRYSRLVQSSAVSRAEYELAATAYRVAQEEQT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARQLVEKGTGARTEDIDAQAAEVRTLEARLAEASLQLADSTLRAPYSGVVAQRFVDEKQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SIVANKPVVTLQNVDEIDIVADVPEAAMAADIRSTAFTKLVAEFSTAPGVQFPVRIKEVA
HHHCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QVADPATQTFKVRVAMPAPRRVNVLPGMTATVAATYRRAGARGRRILVPISAVFKEDTGE
HHCCCCCEEEEEEEECCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCC
QVAWIIGPDQLIGRRLVKMGAATGGQVEILDGLRPGDRIVVAGAPFLSPGMRVRDLGDAL
EEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHC
GGD
CCC
>Mature Secondary Structure 
SKLHNPNLAVALAIAGLAAGCSRPTETRSEAARPVKTMVVTDGDEPLVRTFPGRVEASR
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCHHHHCCCCCCCCC
SVDLAFQVPGLLVNLPVKEGQKVAKGAVIAQPRQDEFQERVKSLQGQVDQARASLSALQV
CEEEEEECCCEEEECCHHHCCHHHCCCEEECCCHHHHHHHHHHHHCHHHHHHHHHHHHHC
GERPEERLSREAQLRAVGAKVANAKTEFDRYSRLVQSSAVSRAEYELAATAYRVAQEEQT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AARQLVEKGTGARTEDIDAQAAEVRTLEARLAEASLQLADSTLRAPYSGVVAQRFVDEKQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
SIVANKPVVTLQNVDEIDIVADVPEAAMAADIRSTAFTKLVAEFSTAPGVQFPVRIKEVA
HHHCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QVADPATQTFKVRVAMPAPRRVNVLPGMTATVAATYRRAGARGRRILVPISAVFKEDTGE
HHCCCCCEEEEEEEECCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEEEHHHHHCCCCCC
QVAWIIGPDQLIGRRLVKMGAATGGQVEILDGLRPGDRIVVAGAPFLSPGMRVRDLGDAL
EEEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHC
GGD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]