Click here to switch to the map view.

The map label for this gene is rhgT [H]

Identifier: 116624835

GI number: 116624835

Start: 7214586

End: 7215293

Strand: Direct

Name: rhgT [H]

Synonym: Acid_5759

Alternate gene names: 116624835

Gene position: 7214586-7215293 (Clockwise)

Preceding gene: 116624825

Following gene: 116624836

Centisome position: 72.39

GC content: 63.7

Gene sequence:

>708_bases
ATGCGTTGCTCCTTTCTAATAGCGGCTCTGTTCGCAGCTTCCCTGTTTGCGGCTGAGCCGCCTCTTTCCCCGGTGCGGGT
AGTCCTGGTCGGCGATTCCACCGTCAACGACGAGGGCGGGTGGGGTCCCGGCTTTCGCGCCTTTTTCGGCCCTGAGCTCC
AGGTGGTCAACCTGGCCAGGAACGGCCGCAGTTCCAAAAGCTTCCGGTCCGAGGGGCTCTGGGCTCCCGCCATCGCGGGC
AAGCCCGATTACATTCTGATTCAATTCGGCCACAACGATGGTCCCGGCAAAGGTGCGGATCGCGAAACCGACCCCAAAAC
CACCTTCCGTGAAAACATGACCCGCTACATCGAGGAAGCCCGCGCCGCCGGAGCCCAGCCAGTCCTGGTGACCTCGATCG
TCCGCCGGACCTTCACCCCGGACGGCAAAATCAAACCGGATTCCCTGCTTCCTTATGTGGAGGCCATCCGCGAACTCGCC
GCGGCACAGAAATTACCCCTGATCGACCTCTACTCGCTGACCGTGGCCCAAGCCGAGCAGCTTGGCCCTGATGGCTGCGG
CGATATCGACGCGCGGCTGCCCGATGGCAAGCGCGACCACACCCACCTGGGTCTCAAAGGCCGCCAGGAAATCGGGCGCA
TGGCCGCGCAGGAATTTGTGAAACTGATCCCCGCCGTGCGCAGCTACACCGCGCCGGCTCTGCCCTAG

Upstream 100 bases:

>100_bases
TGCGGTTAGTCGCGCACTATACGTTACTTCTACCACTGCGACGTGGATTTTTTGCACGAAGCGGAATATCGTACCTGTGG
TCTCCACCGGTCTCCACCAC

Downstream 100 bases:

>100_bases
GAGAAATGCAAATGAAGATCCGATACCTGGTTGTATTGTTCGCCCTGACGCTTCCAATTTCGATGTTCTCGCAGGAGTTT
CGCGGTACCATCTCCGGCGT

Product: GDSL family lipase

Products: NA

Alternate protein names: RGAE [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MRCSFLIAALFAASLFAAEPPLSPVRVVLVGDSTVNDEGGWGPGFRAFFGPELQVVNLARNGRSSKSFRSEGLWAPAIAG
KPDYILIQFGHNDGPGKGADRETDPKTTFRENMTRYIEEARAAGAQPVLVTSIVRRTFTPDGKIKPDSLLPYVEAIRELA
AAQKLPLIDLYSLTVAQAEQLGPDGCGDIDARLPDGKRDHTHLGLKGRQEIGRMAAQEFVKLIPAVRSYTAPALP

Sequences:

>Translated_235_residues
MRCSFLIAALFAASLFAAEPPLSPVRVVLVGDSTVNDEGGWGPGFRAFFGPELQVVNLARNGRSSKSFRSEGLWAPAIAG
KPDYILIQFGHNDGPGKGADRETDPKTTFRENMTRYIEEARAAGAQPVLVTSIVRRTFTPDGKIKPDSLLPYVEAIRELA
AAQKLPLIDLYSLTVAQAEQLGPDGCGDIDARLPDGKRDHTHLGLKGRQEIGRMAAQEFVKLIPAVRSYTAPALP
>Mature_235_residues
MRCSFLIAALFAASLFAAEPPLSPVRVVLVGDSTVNDEGGWGPGFRAFFGPELQVVNLARNGRSSKSFRSEGLWAPAIAG
KPDYILIQFGHNDGPGKGADRETDPKTTFRENMTRYIEEARAAGAQPVLVTSIVRRTFTPDGKIKPDSLLPYVEAIRELA
AAQKLPLIDLYSLTVAQAEQLGPDGCGDIDARLPDGKRDHTHLGLKGRQEIGRMAAQEFVKLIPAVRSYTAPALP

Specific function: May play role in the degradation of type I rhamnogalacturonan derived from plant cell walls. This enzyme has a broad substrate specificity, and shows strong preference for glucose pentaacetate, beta-naphthylacetate, and p-nitrophenyl acetate (pNPA). Also

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: NA

Molecular weight: Translated: 25411; Mature: 25411

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCSFLIAALFAASLFAAEPPLSPVRVVLVGDSTVNDEGGWGPGFRAFFGPELQVVNLAR
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEECCCCCCCCCCHHHCCCCEEEEEECC
NGRSSKSFRSEGLWAPAIAGKPDYILIQFGHNDGPGKGADRETDPKTTFRENMTRYIEEA
CCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RAAGAQPVLVTSIVRRTFTPDGKIKPDSLLPYVEAIRELAAAQKLPLIDLYSLTVAQAEQ
HHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGPDGCGDIDARLPDGKRDHTHLGLKGRQEIGRMAAQEFVKLIPAVRSYTAPALP
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRCSFLIAALFAASLFAAEPPLSPVRVVLVGDSTVNDEGGWGPGFRAFFGPELQVVNLAR
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEECCCCCCCCCCHHHCCCCEEEEEECC
NGRSSKSFRSEGLWAPAIAGKPDYILIQFGHNDGPGKGADRETDPKTTFRENMTRYIEEA
CCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RAAGAQPVLVTSIVRRTFTPDGKIKPDSLLPYVEAIRELAAAQKLPLIDLYSLTVAQAEQ
HHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LGPDGCGDIDARLPDGKRDHTHLGLKGRQEIGRMAAQEFVKLIPAVRSYTAPALP
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]