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The map label for this gene is tilS/hprT [H]

Identifier: 116624741

GI number: 116624741

Start: 7097775

End: 7098308

Strand: Direct

Name: tilS/hprT [H]

Synonym: Acid_5665

Alternate gene names: 116624741

Gene position: 7097775-7098308 (Clockwise)

Preceding gene: 116624740

Following gene: 116624742

Centisome position: 71.22

GC content: 57.68

Gene sequence:

>534_bases
ATGACCGATCCCAAGCTCCGTATCCTGATCTCCGCCGAGAAGATTCAGGAGCGCATCCGCGAACTCGGCGAAAAGATTTC
GGCCGACTATCCGACCGGTAATCTGCACATGATCTGCATCTTGAAAGGGGCGATCTTTTTCATGACCGACCTGGCGCGCG
CGATGAAGCGCGACGTGCAGATCGATTTCATGGGCATTTCGACTTACGGCAAGGGCAAGACCTCGTCGGGCGAAGTCAAG
GTCACCAAGGATCTGGATATTTCCCTGGAAGGCGCCGATGTTTTGATTGTCGAAGACATCGTGGATAGCGGCGTCACGCT
CAATTACCTGATGCACCTGCTGGATCAGCGCAAGCCGCACTCCATCCGCATCGCCGCCCTGCTGGATAAGCCCGAACGGC
GTTTGCGTCCGGTGCATGTGAGCTACGTGGGCTTCGAAATCCCGGACCAGTTCGTGGTGGGCTACGGCCTGGATTACGCG
GAGAAGTACCGCAATCTCGACGACGTCTGCGTGCTCGACGATCCCGGCGCGTAA

Upstream 100 bases:

>100_bases
TTCGCGTTCCGGCAGGCGGTACCCGGAAAAGCCACAGGCGCGCATATCCACATCGGTCCCTTGAGCACGAGATACAAACT
CGGCGGATAATGGTTTCTCC

Downstream 100 bases:

>100_bases
GCAACAATCCGGTCCGAACCGCGCGCGTTAGCGAGCGGTCGCTAATTCGCATTGAATCTCGCGGCGACGGTACTAGGATT
CGCGGCGTTCCGGTGATACA

Product: hypoxanthine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MTDPKLRILISAEKIQERIRELGEKISADYPTGNLHMICILKGAIFFMTDLARAMKRDVQIDFMGISTYGKGKTSSGEVK
VTKDLDISLEGADVLIVEDIVDSGVTLNYLMHLLDQRKPHSIRIAALLDKPERRLRPVHVSYVGFEIPDQFVVGYGLDYA
EKYRNLDDVCVLDDPGA

Sequences:

>Translated_177_residues
MTDPKLRILISAEKIQERIRELGEKISADYPTGNLHMICILKGAIFFMTDLARAMKRDVQIDFMGISTYGKGKTSSGEVK
VTKDLDISLEGADVLIVEDIVDSGVTLNYLMHLLDQRKPHSIRIAALLDKPERRLRPVHVSYVGFEIPDQFVVGYGLDYA
EKYRNLDDVCVLDDPGA
>Mature_176_residues
TDPKLRILISAEKIQERIRELGEKISADYPTGNLHMICILKGAIFFMTDLARAMKRDVQIDFMGISTYGKGKTSSGEVKV
TKDLDISLEGADVLIVEDIVDSGVTLNYLMHLLDQRKPHSIRIAALLDKPERRLRPVHVSYVGFEIPDQFVVGYGLDYAE
KYRNLDDVCVLDDPGA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=180, Percent_Identity=36.6666666666667, Blast_Score=111, Evalue=4e-25,
Organism=Homo sapiens, GI9910262, Length=179, Percent_Identity=35.7541899441341, Blast_Score=109, Evalue=1e-24,
Organism=Escherichia coli, GI87081689, Length=163, Percent_Identity=46.0122699386503, Blast_Score=151, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17510817, Length=178, Percent_Identity=33.1460674157303, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 19884; Mature: 19753

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPKLRILISAEKIQERIRELGEKISADYPTGNLHMICILKGAIFFMTDLARAMKRDVQ
CCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCE
IDFMGISTYGKGKTSSGEVKVTKDLDISLEGADVLIVEDIVDSGVTLNYLMHLLDQRKPH
EEEEEEEECCCCCCCCCCEEEEECCEEEECCCCEEEEEHHHCCCCHHHHHHHHHHCCCCC
SIRIAALLDKPERRLRPVHVSYVGFEIPDQFVVGYGLDYAEKYRNLDDVCVLDDPGA
EEEEEEEECCCHHHCCCEEEEEEEEECCCCEEEECCCCHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure 
TDPKLRILISAEKIQERIRELGEKISADYPTGNLHMICILKGAIFFMTDLARAMKRDVQ
CCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCE
IDFMGISTYGKGKTSSGEVKVTKDLDISLEGADVLIVEDIVDSGVTLNYLMHLLDQRKPH
EEEEEEEECCCCCCCCCCEEEEECCEEEECCCCEEEEEHHHCCCCHHHHHHHHHHCCCCC
SIRIAALLDKPERRLRPVHVSYVGFEIPDQFVVGYGLDYAEKYRNLDDVCVLDDPGA
EEEEEEEECCCHHHCCCEEEEEEEEECCCCEEEECCCCHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]