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The map label for this gene is nfdA [H]

Identifier: 116624731

GI number: 116624731

Start: 7087538

End: 7089010

Strand: Reverse

Name: nfdA [H]

Synonym: Acid_5655

Alternate gene names: 116624731

Gene position: 7089010-7087538 (Counterclockwise)

Preceding gene: 116624734

Following gene: 116624729

Centisome position: 71.13

GC content: 67.96

Gene sequence:

>1473_bases
ATGAGACTGCGGCTCGCCATCGCGCTCCTCTTCCTCCGCGCCACCGCCGCCGCGCCCGCGACTCTCGCGATCGTCAACGC
CCGCGTCTGGACCGGCGACCCGGCGCGGCCCTTCGCGGAAGCCATTGCAACCTCCGGCGATCGCGTCCTCGCCGTGGGGT
CGAACGCCGGCATTCGCGCGCTCGCCGCCGGCACCGTCATCGATGGCGCAGGCGCCACGCTCACCCCAGGCTTCATCGAT
TCCCACATTCACCTCCTCGTCTTCGATCGCAACCGTCCCTACCCGCCCATCTTCATGCGCTACCTCACCACTCGCCGGCA
GGTAGCCGACCGCATCTCCGCCTACGCCGCCAAGCTTCCCAAAGGCGCCTGGATCCTCGGCGAGGATTGGACCGACGCGC
TGTGGGCCGTTCAGCCTCCTTCGCGCGCCTGGCTCGACCGCATCGCACCCGATCACCCCGTCTGGCTCACCCGCATCGAA
GGCGCGTCCGGCATCGCCAACAGCGCCGCGCTTCGTGCCGCGGGCTTCGGCGCCCAAACGCCCGGCCTGGTTGGCGGCGG
TAACATGTGGCGCATCGAAGCTGCCATCATCGAACCATTCCGCGAGCGCGACGAGCGCATCGTCGAACACGCGATGGATC
TGTTGCTTCGCGCGGGCGTCACCTCCGCGCAGCACAACAATGCCTGGTACGATTTCCTGATCCTGCGCCGCCTGCACCAG
GCCGGACGGCGCCGCATCCGCGTTTACGACTCGCCCCCGCTGCCCGCCTGGGAACGCCTGCGCGACTACATCCGCGAGTT
TGGCCGCGGCGATGCCTGGATGCATTGGGGCGGCCTCAAAGGCTTCACCGTAGTCACCGAACCTGACTATTACCGCTGGG
TTTCCGGCGCCTCAAGCTGCGGACTCCAGGTGATGGTGCACCTCGGCTCCGAAGCCGAGCTCCGCACGCTGCTCGGTGTC
TTCCTGCGCGTGCGCCGTGAACAGCATCTGGCCGACCCGCGCTTTCGCCTCGAACACGCGCACGACCTTCCGCCCGATGT
TATCCCCCTGGTCGCCGAAGCCCGAGCCATCCCCAGCTGGCAGCCTGCGCTGCTCGCGCACTTCGCCGCTCGCACCGCCG
CCGGCCAGCTCGCGCCCAGGAATCTCTTTCCTGCCCGAGCGGTTCTCGATGCCGGTGTGAAGATCGCCTTCGGTACCGAT
TCCTCGCCCGCCTATTCGCTCGTCGCGCCGGTGCAAACCCTCCAGATCGCCATGGAACACGCCATGAGCCTGGACCAGGC
CCTCCGCGCCCACACCCTGGACGCCGCCTACGCCGAATTCGCCGAAAACGAGAAGGGATCGCTGCAAGCCGGGAAGCTCG
CCGACTTCGTCCTGTTCGACCGCGATCTCTCGCGCCTTCCCGTCCACCAGATCCACGAGGCGAAGGTTCGCCTCACCGTG
GTCGGCGGCCGCATCATGTACGATTCGCGCTAG

Upstream 100 bases:

>100_bases
CAACTCAAGTTTGTCCGACATACGGCCGGTTCCTCGAACCTGCAAGTCTATCCAAATTTCTCGCGGGAAACTGTTTCGGA
TATCCTGGTGTCAAATAAAA

Downstream 100 bases:

>100_bases
CCGCCCGCAACTCCACCATCGGGCGCCCCTTTGCTTGCCGCCCCACGGCGCTCACCTCCAGCCCCGCATCCCGCGCGATC
GCCCGGAGCTGCTCCAGAGA

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRLRLAIALLFLRATAAAPATLAIVNARVWTGDPARPFAEAIATSGDRVLAVGSNAGIRALAAGTVIDGAGATLTPGFID
SHIHLLVFDRNRPYPPIFMRYLTTRRQVADRISAYAAKLPKGAWILGEDWTDALWAVQPPSRAWLDRIAPDHPVWLTRIE
GASGIANSAALRAAGFGAQTPGLVGGGNMWRIEAAIIEPFRERDERIVEHAMDLLLRAGVTSAQHNNAWYDFLILRRLHQ
AGRRRIRVYDSPPLPAWERLRDYIREFGRGDAWMHWGGLKGFTVVTEPDYYRWVSGASSCGLQVMVHLGSEAELRTLLGV
FLRVRREQHLADPRFRLEHAHDLPPDVIPLVAEARAIPSWQPALLAHFAARTAAGQLAPRNLFPARAVLDAGVKIAFGTD
SSPAYSLVAPVQTLQIAMEHAMSLDQALRAHTLDAAYAEFAENEKGSLQAGKLADFVLFDRDLSRLPVHQIHEAKVRLTV
VGGRIMYDSR

Sequences:

>Translated_490_residues
MRLRLAIALLFLRATAAAPATLAIVNARVWTGDPARPFAEAIATSGDRVLAVGSNAGIRALAAGTVIDGAGATLTPGFID
SHIHLLVFDRNRPYPPIFMRYLTTRRQVADRISAYAAKLPKGAWILGEDWTDALWAVQPPSRAWLDRIAPDHPVWLTRIE
GASGIANSAALRAAGFGAQTPGLVGGGNMWRIEAAIIEPFRERDERIVEHAMDLLLRAGVTSAQHNNAWYDFLILRRLHQ
AGRRRIRVYDSPPLPAWERLRDYIREFGRGDAWMHWGGLKGFTVVTEPDYYRWVSGASSCGLQVMVHLGSEAELRTLLGV
FLRVRREQHLADPRFRLEHAHDLPPDVIPLVAEARAIPSWQPALLAHFAARTAAGQLAPRNLFPARAVLDAGVKIAFGTD
SSPAYSLVAPVQTLQIAMEHAMSLDQALRAHTLDAAYAEFAENEKGSLQAGKLADFVLFDRDLSRLPVHQIHEAKVRLTV
VGGRIMYDSR
>Mature_490_residues
MRLRLAIALLFLRATAAAPATLAIVNARVWTGDPARPFAEAIATSGDRVLAVGSNAGIRALAAGTVIDGAGATLTPGFID
SHIHLLVFDRNRPYPPIFMRYLTTRRQVADRISAYAAKLPKGAWILGEDWTDALWAVQPPSRAWLDRIAPDHPVWLTRIE
GASGIANSAALRAAGFGAQTPGLVGGGNMWRIEAAIIEPFRERDERIVEHAMDLLLRAGVTSAQHNNAWYDFLILRRLHQ
AGRRRIRVYDSPPLPAWERLRDYIREFGRGDAWMHWGGLKGFTVVTEPDYYRWVSGASSCGLQVMVHLGSEAELRTLLGV
FLRVRREQHLADPRFRLEHAHDLPPDVIPLVAEARAIPSWQPALLAHFAARTAAGQLAPRNLFPARAVLDAGVKIAFGTD
SSPAYSLVAPVQTLQIAMEHAMSLDQALRAHTLDAAYAEFAENEKGSLQAGKLADFVLFDRDLSRLPVHQIHEAKVRLTV
VGGRIMYDSR

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 54017; Mature: 54017

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRLAIALLFLRATAAAPATLAIVNARVWTGDPARPFAEAIATSGDRVLAVGSNAGIRA
CCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCEEE
LAAGTVIDGAGATLTPGFIDSHIHLLVFDRNRPYPPIFMRYLTTRRQVADRISAYAAKLP
EEECCEECCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KGAWILGEDWTDALWAVQPPSRAWLDRIAPDHPVWLTRIEGASGIANSAALRAAGFGAQT
CCCEEECCCCCCCEEEECCCHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCC
PGLVGGGNMWRIEAAIIEPFRERDERIVEHAMDLLLRAGVTSAQHNNAWYDFLILRRLHQ
CCEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AGRRRIRVYDSPPLPAWERLRDYIREFGRGDAWMHWGGLKGFTVVTEPDYYRWVSGASSC
HCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCHHHHHCCCCCC
GLQVMVHLGSEAELRTLLGVFLRVRREQHLADPRFRLEHAHDLPPDVIPLVAEARAIPSW
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCC
QPALLAHFAARTAAGQLAPRNLFPARAVLDAGVKIAFGTDSSPAYSLVAPVQTLQIAMEH
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
AMSLDQALRAHTLDAAYAEFAENEKGSLQAGKLADFVLFDRDLSRLPVHQIHEAKVRLTV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHCCHHHHCCCEEEEEE
VGGRIMYDSR
ECCEEEECCC
>Mature Secondary Structure
MRLRLAIALLFLRATAAAPATLAIVNARVWTGDPARPFAEAIATSGDRVLAVGSNAGIRA
CCHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCEEE
LAAGTVIDGAGATLTPGFIDSHIHLLVFDRNRPYPPIFMRYLTTRRQVADRISAYAAKLP
EEECCEECCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
KGAWILGEDWTDALWAVQPPSRAWLDRIAPDHPVWLTRIEGASGIANSAALRAAGFGAQT
CCCEEECCCCCCCEEEECCCHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCC
PGLVGGGNMWRIEAAIIEPFRERDERIVEHAMDLLLRAGVTSAQHNNAWYDFLILRRLHQ
CCEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AGRRRIRVYDSPPLPAWERLRDYIREFGRGDAWMHWGGLKGFTVVTEPDYYRWVSGASSC
HCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEEECCCHHHHHCCCCCC
GLQVMVHLGSEAELRTLLGVFLRVRREQHLADPRFRLEHAHDLPPDVIPLVAEARAIPSW
CEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCC
QPALLAHFAARTAAGQLAPRNLFPARAVLDAGVKIAFGTDSSPAYSLVAPVQTLQIAMEH
CHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
AMSLDQALRAHTLDAAYAEFAENEKGSLQAGKLADFVLFDRDLSRLPVHQIHEAKVRLTV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEECCCHHHCCHHHHCCCEEEEEE
VGGRIMYDSR
ECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA