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The map label for this gene is metB [H]

Identifier: 116624719

GI number: 116624719

Start: 7073421

End: 7074545

Strand: Reverse

Name: metB [H]

Synonym: Acid_5643

Alternate gene names: 116624719

Gene position: 7074545-7073421 (Counterclockwise)

Preceding gene: 116624726

Following gene: 116624716

Centisome position: 70.99

GC content: 66.67

Gene sequence:

>1125_bases
ATGAAAATCGAGACCCGCGCCGTCCACGCCGGACGCCATATCGACCCCGCCACCGGCGCCGTCACCAGCCCGATCCACCT
TTCCACCACGTTCGAGCGCAGCGAAGCCGGTGAATATCCGCGCGGCTTCAGCTACTCGCGCGAAGACAACCCCAACCGCC
GCGCGCTGGAGGAATGCCTCGCGGCGCTCGAGGGCGGCCGGCAGGCGATCTGCTTTTCCAGCGGCCTTGCGGTAGTCACG
GCGCTAGTCCAGGGCCTGGAGCCTGGCGACCACATCGTCGCGCCGGACGACGTGTATTGGGGACTGCGCAAGGTGATCGG
CGAAGTCTTCGGCAAGTGGGGCCTGGAGACCAGCTACGTGGACATGACCAACATCGACGATTTCCACGCCGCGCTGCGCG
CCAATACCCGGCTGGTCTGGGTGGAAACGCCCTCGAATCCGCTGATGAAGGTCACCGACCTGACTGCGATTGTCGCGCTC
ACCCGGCAGGCAAGTCCGAACATCGTGACCGTCTGCGACGGCACCTTCGCGACGCCGGTCCTGCAGCAGCCGCTCGATTG
CGGCATCGACATGGTGGCGCACTCCACCACGAAGTACCTGGGCGGCCACAGCGATGTCATCGGCGGCGCGCTGATCACGC
GGCACGAGAATTACCTGTTCGAGCGCGCGCGCAAATCGCAGCGCTACGGCGGCAGCGTGCCCTCGCCCTTCGATTGCTGG
CTGACCATGCGCGGCATCGATACGCTGCCGTGGCGGGTGCGCGCGCAATCGGAAAACGCGATGAAGGTGGCGCGATTCCT
GGCGGCCCATCATGCCGTGGAACGGGTGCACTACCCGGGCCTGCCCGATCATCCCGGACACAAGGTCGCCGCCCGCCAGA
TGTCGGCATTCGGAGGGATGCTTTCGTTCCAGGTGCGCGGCACGCGCGAGACGGCGCTGGGTGCCGCGGCCAAGTGCAAG
CTCTTCATCCGCGCGACCAGCCTCGGCGGGGCGCACAGCTTAATTGAACATCGCGCCAGCGTGGAGGGTCCGGATACACG
CACGCCGCAGAATCTTCTGCGCCTCTCGATCGGCCTGGAGCACGCCGACGATCTCATCGCCGACCTCGCGCACGCGCTGG
AGTAG

Upstream 100 bases:

>100_bases
TAGCAGAAAACAAGAGGTAAAACTCGACAACCGAGCTCTGGTGGGTGGGGCATACCGCGCAGCGGTGGGGCAGGGTGTCG
GGACGCTACACTAGGCAGTG

Downstream 100 bases:

>100_bases
CTCGTTAACTACATCTTGCGGTACGCCAGGGATGCCGCGGAGATTTCCGTGCCGCCGGGCAGCTGGGCTTCCTGCGGGGT
GCCGTTGAAATCCATCTCGT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKIETRAVHAGRHIDPATGAVTSPIHLSTTFERSEAGEYPRGFSYSREDNPNRRALEECLAALEGGRQAICFSSGLAVVT
ALVQGLEPGDHIVAPDDVYWGLRKVIGEVFGKWGLETSYVDMTNIDDFHAALRANTRLVWVETPSNPLMKVTDLTAIVAL
TRQASPNIVTVCDGTFATPVLQQPLDCGIDMVAHSTTKYLGGHSDVIGGALITRHENYLFERARKSQRYGGSVPSPFDCW
LTMRGIDTLPWRVRAQSENAMKVARFLAAHHAVERVHYPGLPDHPGHKVAARQMSAFGGMLSFQVRGTRETALGAAAKCK
LFIRATSLGGAHSLIEHRASVEGPDTRTPQNLLRLSIGLEHADDLIADLAHALE

Sequences:

>Translated_374_residues
MKIETRAVHAGRHIDPATGAVTSPIHLSTTFERSEAGEYPRGFSYSREDNPNRRALEECLAALEGGRQAICFSSGLAVVT
ALVQGLEPGDHIVAPDDVYWGLRKVIGEVFGKWGLETSYVDMTNIDDFHAALRANTRLVWVETPSNPLMKVTDLTAIVAL
TRQASPNIVTVCDGTFATPVLQQPLDCGIDMVAHSTTKYLGGHSDVIGGALITRHENYLFERARKSQRYGGSVPSPFDCW
LTMRGIDTLPWRVRAQSENAMKVARFLAAHHAVERVHYPGLPDHPGHKVAARQMSAFGGMLSFQVRGTRETALGAAAKCK
LFIRATSLGGAHSLIEHRASVEGPDTRTPQNLLRLSIGLEHADDLIADLAHALE
>Mature_374_residues
MKIETRAVHAGRHIDPATGAVTSPIHLSTTFERSEAGEYPRGFSYSREDNPNRRALEECLAALEGGRQAICFSSGLAVVT
ALVQGLEPGDHIVAPDDVYWGLRKVIGEVFGKWGLETSYVDMTNIDDFHAALRANTRLVWVETPSNPLMKVTDLTAIVAL
TRQASPNIVTVCDGTFATPVLQQPLDCGIDMVAHSTTKYLGGHSDVIGGALITRHENYLFERARKSQRYGGSVPSPFDCW
LTMRGIDTLPWRVRAQSENAMKVARFLAAHHAVERVHYPGLPDHPGHKVAARQMSAFGGMLSFQVRGTRETALGAAAKCK
LFIRATSLGGAHSLIEHRASVEGPDTRTPQNLLRLSIGLEHADDLIADLAHALE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=365, Percent_Identity=40.8219178082192, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI299473759, Length=365, Percent_Identity=36.986301369863, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI25453487, Length=365, Percent_Identity=36.1643835616438, Blast_Score=205, Evalue=7e-53,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=37.7952755905512, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1789383, Length=385, Percent_Identity=32.7272727272727, Blast_Score=181, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17560056, Length=366, Percent_Identity=42.6229508196721, Blast_Score=295, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17538055, Length=367, Percent_Identity=42.7792915531335, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI71980708, Length=385, Percent_Identity=37.4025974025974, Blast_Score=244, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6319307, Length=386, Percent_Identity=41.1917098445596, Blast_Score=285, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=28.4653465346535, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321904, Length=392, Percent_Identity=30.6122448979592, Blast_Score=145, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6321170, Length=307, Percent_Identity=29.9674267100977, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=31.1926605504587, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI19922554, Length=379, Percent_Identity=41.9525065963061, Blast_Score=305, Evalue=4e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 40759; Mature: 40759

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIETRAVHAGRHIDPATGAVTSPIHLSTTFERSEAGEYPRGFSYSREDNPNRRALEECL
CCCCCCCHHCCCCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCHHHHHHHH
AALEGGRQAICFSSGLAVVTALVQGLEPGDHIVAPDDVYWGLRKVIGEVFGKWGLETSYV
HHHHCCCEEEEECCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCEE
DMTNIDDFHAALRANTRLVWVETPSNPLMKVTDLTAIVALTRQASPNIVTVCDGTFATPV
ECCCCHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
LQQPLDCGIDMVAHSTTKYLGGHSDVIGGALITRHENYLFERARKSQRYGGSVPSPFDCW
HHCHHHCCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHE
LTMRGIDTLPWRVRAQSENAMKVARFLAAHHAVERVHYPGLPDHPGHKVAARQMSAFGGM
EEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCE
LSFQVRGTRETALGAAAKCKLFIRATSLGGAHSLIEHRASVEGPDTRTPQNLLRLSIGLE
EEEEECCCHHHHHCHHHEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCH
HADDLIADLAHALE
HHHHHHHHHHHHCC
>Mature Secondary Structure
MKIETRAVHAGRHIDPATGAVTSPIHLSTTFERSEAGEYPRGFSYSREDNPNRRALEECL
CCCCCCCHHCCCCCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCHHHHHHHH
AALEGGRQAICFSSGLAVVTALVQGLEPGDHIVAPDDVYWGLRKVIGEVFGKWGLETSYV
HHHHCCCEEEEECCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCEE
DMTNIDDFHAALRANTRLVWVETPSNPLMKVTDLTAIVALTRQASPNIVTVCDGTFATPV
ECCCCHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
LQQPLDCGIDMVAHSTTKYLGGHSDVIGGALITRHENYLFERARKSQRYGGSVPSPFDCW
HHCHHHCCHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHE
LTMRGIDTLPWRVRAQSENAMKVARFLAAHHAVERVHYPGLPDHPGHKVAARQMSAFGGM
EEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCE
LSFQVRGTRETALGAAAKCKLFIRATSLGGAHSLIEHRASVEGPDTRTPQNLLRLSIGLE
EEEEECCCHHHHHCHHHEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCH
HADDLIADLAHALE
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]