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The map label for this gene is apbE [H]

Identifier: 116624718

GI number: 116624718

Start: 7071889

End: 7073412

Strand: Direct

Name: apbE [H]

Synonym: Acid_5642

Alternate gene names: 116624718

Gene position: 7071889-7073412 (Clockwise)

Preceding gene: 116624717

Following gene: 116624720

Centisome position: 70.96

GC content: 67.19

Gene sequence:

>1524_bases
ATGAAAAAGCTCCTTTTTACGTTGACGGGCGCAGGACTGCTCGCTTTCTTCCTGGGCAGTTCGACGGGCGCATCCACCGG
TCCCGACCGGGCCGGCCGGCCATTCACCTATAACTACGAGAACGTGCTGGGTACGTCGCTGGAGCTGAAAGTGCTCGCGG
GGTCTGAGGCTGTCGCGGATAAGGCGGAAGCGGCGGCGCTCGCGGAAATCGATCGCGAGGCCAAGATTCTGAGCGCCTGG
GATTCCGCCAGCGAATTCAGCCGCTGGTCGCACGGCGTGGGCCGGCCGGAGCGGCTATCGCCGGAGTTGCTCGAAGTGCT
GGGGCTCTTCGACCAGTGGAGGGAGCGCAGCGGCGGTGCGCTCGACGCCTCGGCGGAAGCGGTGACCCGCGTCTGGAAGC
AGGCGGCGGCCGAGCGCAGGATGCCCGCGCCAGCCGAACTCGCAGCCGCCGTAACTGCGGTCAAAGGACGACATTGGGAG
TTGGACACGGCGCACGGTACGGCAACGCACCTTGACCGGACCCCGCTCGCGCTCAACTCCTTCGCGAAGAGCTTCATCGT
CGGCCGTGCCGCGGACGCCGCGCTCACCACCGAGGGTGTCACCGCCGCGGTGGTAAATATCGGCGGAGACCTGGTGGTGC
GCGGCAACTGGACGGAACCGGTGAGCGTGGCCGACCCGAAATCGGACGCGGAGAACGCGGAGCCGATCGCGCGCCTGCGG
ATTCGCAATGCGGCGGTGGCGACCAGCGGCAATTATCGCCGCGGCGTAGAGATCGCCGGACGCCACTACTCGCACATCGT
GGATCCGCGGACCGGCCAGCCGGTGGACCACGTGATCTCGGCCACCGTGGTAGCGCTGAATGCGGCCGATGCCGGCGCGC
TCGCCACTACGATGTGCGTGCTGCAGCCCGAGGAGAGTAAGCGGCTCGCCGAACGAACTCCCGGCGTGGAATACCTGCTG
GTGGCGAGCGACGGCACACGCATCGCGAGCAGCGGATGGAAGCGGCTGCAAGCGCCCGGAGTGGCGATGTCGCCCGTGCC
GCCTCCCGCACCGGCCGCCGCGACGTGGGACCCCGCCTTTGAGCTCGCCATCGATCTCGAGATCGCCCGCATCCAGGATA
TGCGCGCGCGCCGTCCGTATGTCGCGGTGTGGATCGAGGACGCCGATAAGTTCCCGGTCCGCACGGTCGCCTTGTGGTTC
CAGCGCCCGCGCTGGCTGCCGGAGCTGAGGGCGTGGTATCGCAGCGACCGCCTGCGTGCCACGACGGAAGGCAACGATAT
CACCAGTTCGATTTCGAGCGCGACGCGTCCGCCCGGAAAGTACAAGCTGAAGTGGGACGGCAAGGACAATAAGGGCAATC
TGGTGAAGGCGGGCAAGTACACCGTCTTCATCGAGGCGGCCCGCGAGCACGGCACCTACCAGATCATGCGCCACGAGATG
GATTTCAACGGCACCCCGCAGGAAGCCCAGCTGCCCGGCGGCACGGAAATCTCCGCGGCATCCCTGGCGTACCGCAAGAT
GTAG

Upstream 100 bases:

>100_bases
AGGTGGTCCGTGTGCGGACGCCCAATCGTCGCTGAAATAGTCGCTGAAATAATGGACCATGGTACAAATGCGACGAATCC
ACCGACCAAGGCATCTGCGA

Downstream 100 bases:

>100_bases
TTAACGAGCTACTCCAGCGCGTGCGCGAGGTCGGCGATGAGATCGTCGGCGTGCTCCAGGCCGATCGAGAGGCGCAGAAG
ATTCTGCGGCGTGCGTGTAT

Product: ApbE family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MKKLLFTLTGAGLLAFFLGSSTGASTGPDRAGRPFTYNYENVLGTSLELKVLAGSEAVADKAEAAALAEIDREAKILSAW
DSASEFSRWSHGVGRPERLSPELLEVLGLFDQWRERSGGALDASAEAVTRVWKQAAAERRMPAPAELAAAVTAVKGRHWE
LDTAHGTATHLDRTPLALNSFAKSFIVGRAADAALTTEGVTAAVVNIGGDLVVRGNWTEPVSVADPKSDAENAEPIARLR
IRNAAVATSGNYRRGVEIAGRHYSHIVDPRTGQPVDHVISATVVALNAADAGALATTMCVLQPEESKRLAERTPGVEYLL
VASDGTRIASSGWKRLQAPGVAMSPVPPPAPAAATWDPAFELAIDLEIARIQDMRARRPYVAVWIEDADKFPVRTVALWF
QRPRWLPELRAWYRSDRLRATTEGNDITSSISSATRPPGKYKLKWDGKDNKGNLVKAGKYTVFIEAAREHGTYQIMRHEM
DFNGTPQEAQLPGGTEISAASLAYRKM

Sequences:

>Translated_507_residues
MKKLLFTLTGAGLLAFFLGSSTGASTGPDRAGRPFTYNYENVLGTSLELKVLAGSEAVADKAEAAALAEIDREAKILSAW
DSASEFSRWSHGVGRPERLSPELLEVLGLFDQWRERSGGALDASAEAVTRVWKQAAAERRMPAPAELAAAVTAVKGRHWE
LDTAHGTATHLDRTPLALNSFAKSFIVGRAADAALTTEGVTAAVVNIGGDLVVRGNWTEPVSVADPKSDAENAEPIARLR
IRNAAVATSGNYRRGVEIAGRHYSHIVDPRTGQPVDHVISATVVALNAADAGALATTMCVLQPEESKRLAERTPGVEYLL
VASDGTRIASSGWKRLQAPGVAMSPVPPPAPAAATWDPAFELAIDLEIARIQDMRARRPYVAVWIEDADKFPVRTVALWF
QRPRWLPELRAWYRSDRLRATTEGNDITSSISSATRPPGKYKLKWDGKDNKGNLVKAGKYTVFIEAAREHGTYQIMRHEM
DFNGTPQEAQLPGGTEISAASLAYRKM
>Mature_507_residues
MKKLLFTLTGAGLLAFFLGSSTGASTGPDRAGRPFTYNYENVLGTSLELKVLAGSEAVADKAEAAALAEIDREAKILSAW
DSASEFSRWSHGVGRPERLSPELLEVLGLFDQWRERSGGALDASAEAVTRVWKQAAAERRMPAPAELAAAVTAVKGRHWE
LDTAHGTATHLDRTPLALNSFAKSFIVGRAADAALTTEGVTAAVVNIGGDLVVRGNWTEPVSVADPKSDAENAEPIARLR
IRNAAVATSGNYRRGVEIAGRHYSHIVDPRTGQPVDHVISATVVALNAADAGALATTMCVLQPEESKRLAERTPGVEYLL
VASDGTRIASSGWKRLQAPGVAMSPVPPPAPAAATWDPAFELAIDLEIARIQDMRARRPYVAVWIEDADKFPVRTVALWF
QRPRWLPELRAWYRSDRLRATTEGNDITSSISSATRPPGKYKLKWDGKDNKGNLVKAGKYTVFIEAAREHGTYQIMRHEM
DFNGTPQEAQLPGGTEISAASLAYRKM

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=268, Percent_Identity=26.4925373134328, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 54856; Mature: 54856

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLFTLTGAGLLAFFLGSSTGASTGPDRAGRPFTYNYENVLGTSLELKVLAGSEAVAD
CCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHCCCCEEEEEEECCHHHHH
KAEAAALAEIDREAKILSAWDSASEFSRWSHGVGRPERLSPELLEVLGLFDQWRERSGGA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LDASAEAVTRVWKQAAAERRMPAPAELAAAVTAVKGRHWELDTAHGTATHLDRTPLALNS
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCHHHHH
FAKSFIVGRAADAALTTEGVTAAVVNIGGDLVVRGNWTEPVSVADPKSDAENAEPIARLR
HHHHHHHCCCCCCEEECCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHE
IRNAAVATSGNYRRGVEIAGRHYSHIVDPRTGQPVDHVISATVVALNAADAGALATTMCV
ECCEEEECCCCCCCCCEECCCCHHHCCCCCCCCCHHHHHHHHEEEEECCCCCHHHHEEEE
LQPEESKRLAERTPGVEYLLVASDGTRIASSGWKRLQAPGVAMSPVPPPAPAAATWDPAF
ECCHHHHHHHHHCCCCEEEEEECCCCEEHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCE
ELAIDLEIARIQDMRARRPYVAVWIEDADKFPVRTVALWFQRPRWLPELRAWYRSDRLRA
EEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCEEE
TTEGNDITSSISSATRPPGKYKLKWDGKDNKGNLVKAGKYTVFIEAAREHGTYQIMRHEM
ECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEEEEECCCCCCEEEEEEEC
DFNGTPQEAQLPGGTEISAASLAYRKM
CCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKKLLFTLTGAGLLAFFLGSSTGASTGPDRAGRPFTYNYENVLGTSLELKVLAGSEAVAD
CCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECHHHHCCCCEEEEEEECCHHHHH
KAEAAALAEIDREAKILSAWDSASEFSRWSHGVGRPERLSPELLEVLGLFDQWRERSGGA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
LDASAEAVTRVWKQAAAERRMPAPAELAAAVTAVKGRHWELDTAHGTATHLDRTPLALNS
CCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCCHHHCCCCCHHHHH
FAKSFIVGRAADAALTTEGVTAAVVNIGGDLVVRGNWTEPVSVADPKSDAENAEPIARLR
HHHHHHHCCCCCCEEECCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHE
IRNAAVATSGNYRRGVEIAGRHYSHIVDPRTGQPVDHVISATVVALNAADAGALATTMCV
ECCEEEECCCCCCCCCEECCCCHHHCCCCCCCCCHHHHHHHHEEEEECCCCCHHHHEEEE
LQPEESKRLAERTPGVEYLLVASDGTRIASSGWKRLQAPGVAMSPVPPPAPAAATWDPAF
ECCHHHHHHHHHCCCCEEEEEECCCCEEHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCE
ELAIDLEIARIQDMRARRPYVAVWIEDADKFPVRTVALWFQRPRWLPELRAWYRSDRLRA
EEEEEEEHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCEEE
TTEGNDITSSISSATRPPGKYKLKWDGKDNKGNLVKAGKYTVFIEAAREHGTYQIMRHEM
ECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCEEEEEEECCCCCCEEEEEEEC
DFNGTPQEAQLPGGTEISAASLAYRKM
CCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]