| Definition | Candidatus Solibacter usitatus Ellin6076 chromosome, complete genome. |
|---|---|
| Accession | NC_008536 |
| Length | 9,965,640 |
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The map label for this gene is ampH [H]
Identifier: 116624715
GI number: 116624715
Start: 7067930
End: 7070791
Strand: Direct
Name: ampH [H]
Synonym: Acid_5639
Alternate gene names: 116624715
Gene position: 7067930-7070791 (Clockwise)
Preceding gene: 116624714
Following gene: 116624717
Centisome position: 70.92
GC content: 65.93
Gene sequence:
>2862_bases ATGAACAAACAGCAATTCCGCGTACTCTACCGCGAGTTTCTCTTTCGCATGGTGGATCTGGAAGTGCTCTCCGCCCACGC CCAGGGAGATTCCCACAGACTGCTCGGGCAGTTCGCCGCGCTGCTGCTCTTTATCAGCAACCTGCTCTCGATCGCAGCCT TCGGCTTCGCCGGCCTCCGGATGCCCCCGGCCGCACTGCTCGGGTTCACGATGGTCACCGAGCATTTCCTGATTGCGACC ACCATGCTGGTGGTCGGCCTATTCGCCGTTCTGAGTTGGGATTCCACCTTTCCCGACCGGCGTGACGTCCTCGTGCTCGC CCCCTTGCCGGTGCGCGCCCGGACAATGTTCCTCGCCAAGGTCGCGGCGGTGGCTACGGCCCTCACGCTTACGGTCGCCC TGCTTCACGGCGCCAGCGGCTTGATTTGGCCGCTGGCCTTCGCCTCCCAGGCCACCGCGCAAACCGTTCCGGCGCTCACG CCGGATCCGACGCCGGTTCCGGTGGAAGCCGCGGACCTGCAGTCCGTGATGGATCGCGACCTGGCCGGGCCGTTGAAGAA CGGCGCACTGGCGCCGGGCACCGGTGTCGGCCTCGCCATCGGCGTCTCCAAACACGGCGTGCGCCGCGTGTTCGCTTACG GCGTCGCCAAGCCCGACTCGTTCTTCGAAATCGCGTCCATCACCAAGACCTTCACCGGCCTGATCCTGGCCCGCATGGCG GCGGAAGGCAAGGTGCGGCTCGACGAACCGGTCCGCGAACTGCTGCCCGCCGGGACGGTGCCCAAACCCCGAGGGCAAGA GATCACCTTGCTCGACCTTGCGACGCAGCACTCCGGCCTGCCGCCGATGCCCAGCAACTTCCATCCGGCCGACCGCTCCA ACCCGCATGCCGACTACACCGTCGAGGCGCTGTATGAATATATCGGAATGCACGGCGTCGCCAAGCCCGAGGAAACTAAG TTCGTGTACAGCAATCTCGGTCTCGGCCTGCTGGGGCAGGCTCTGGCGAATCGCGCCGGAGTGAGCTATCGCGACCTGCT TCGCCAGGAGATCACCGAGCCCCTCGGTTTGCGCGATACGGCGATCGCGCTCTCCCCCGAGCAACGGCAGCGCTTCCTGC AAGGCACCAATCCCGAGCACCGCCCGGTGCACGAGTGGGACGTCAACGGCCTGGCGCCCGCCGGAGCGATTCACGCCACT GCGCCCGACATGTTGACCTACCTGGAAGCCAACCTGCACCCCGCGAATTTCCCGGCGCTCGCCCAAGCGTTTGCTGAATC GCACCGCCTGCACGCCGAGGCCACGGGCGGCATGCAGATCGCGTTGGCCTGGCTTTACCGCGCCGATAGCGGAACCTACT GGCACAACGGCGCGACCGCAGGCTTCACTAGCCACGCCTTCTTCGACCCGAATCACGATACCGCGGCCGTCGTGCTGGCC AATAGCGGGCCGAACCTCCTCAACAATCCCGACCTGATCGGCGAGCACATTCGCCAGCGCCTGGCGGGCGAGCCGGCGAT CTCGCTGGATGCCGCATTCGTTCCCGCCGGCAGCGGACTCTCCGGCGCTCTGCGCTGGTTCTTCGCCTACTGGTTCACCA TGCTCGCATCCGGCGTGTTCATTTATTGCGGCGTCCTGGGCTTGCAGGGGCTGGCCGCCCAACTGCTTCCGCGCCGCCTG TTCCTCCGCGTCTCCGGGCTGCTCCAATTGACCGCCTTCTGCCTCTTCGTCTCCGTGTACTTCCTGCAGCCGGTATTCAG CGGGATGGACTCGCTGCTGGAGCCCGCCACTCGCCGCCTGCTCCCCTGGCTGCCCACGTACTGGTTCCTCGGCCTCTTCC ACAGCCTGAATGGATCGATGCACCCGGCGCTGGCAATGCTGGCGCGGCGCGCGTGGATGGGCCTCGGGATCGTCTTGTGC GGAACCGCGGCTGCCTACGCGCTCTCTTACCTGCGCACCCTCCGCAAGATCGTGGAGGAGGCCGACATCACGCCCGGCGC TCGCGGCTGGAACTGGCTGCCGCATTTCGGAAGCCGCGTTCAGACCGCCGTGGGCCAGTTTGGCGTGCGCACACTGGCGC GCAGCCGGCAGCATCGCGTGATCCTGGCCTTCTATTTCGGGATCGGCTTCGCGCTGACGATTTTTCTATTGAAGGCTCCG CTGAAGGCGCCGGTTCCGGGCGCGGCCAGCGACCCTTGGCACCAGGTGAACCCGGAAGTGCTGGCGGCAAGTATCGCGAT GATGACGCTCGGCATCGTGGGGACGCGCGTGGTCTTCGCGATGCCCTTGGAGTTGCGTGCGAACTGGATCTTTCGAGTCA CCGGTGTGCGCGGCGGCTCCCAAGTACTGGCGGCCAGCCGGCGTTCGCTGGTGATGCTTTCCGCCGGGCCGGTCTGGGTT GCGACGGCCGTACTGTGCTTCAGCCTCTGGCCCTGGCGGCAGGCGGCGGGACACGTCGTGCTGCTCGGGCTAATCGGTAT CCTGCTTACCGATCTGTGCCTGTACGGATTTCGTAAGATCCCGTTTACGTGCTCTTACCTGCCGGGCAAATCGCAGGTCC ACATGGTCTTCCTGGGCGCTCTCGGACTGCTGTATTTCATCATGCTCAGCGTCAAGTTCGAGCGCGAAATGCTGTTGCAG GCGCGCAGTACGGCCGCGATGCTGGCGCCGTTCGCCCTGGCGGCAATCGGCTTCCGGGTGTGGACCACCTTGACCGGTGC CGGCGAAGATGAACTGCAGTTTGAAGAAGCGCGAGAACCCGCGGTACTGGAACTGGGCCTGCACCGCGATGGCGTGATGC CGCTGGGGCCGCCGCCCGTGCCTGCAGCCTGGAGATGCGGCGTCAACGAGCCGCGACCGTGA
Upstream 100 bases:
>100_bases TGAGCGCGCCCAACCTCGAAGGCATCTTCTCGCAACTGGCCGTGGAGCAGGATACCGCGTCGATCTCGCGCGAGATCGCG GACCTGATTAAGGCATAGCC
Downstream 100 bases:
>100_bases GCTGAGGGAGCGTTACGATCCGTGGCGTGAGGGTAGCGCTCCCTCACGGTCGCGGCTCGTTGTCAGCAACCGGCGAAGGC GGTTTGCCAGGCTGGCGGCG
Product: beta-lactamase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 953; Mature: 953
Protein sequence:
>953_residues MNKQQFRVLYREFLFRMVDLEVLSAHAQGDSHRLLGQFAALLLFISNLLSIAAFGFAGLRMPPAALLGFTMVTEHFLIAT TMLVVGLFAVLSWDSTFPDRRDVLVLAPLPVRARTMFLAKVAAVATALTLTVALLHGASGLIWPLAFASQATAQTVPALT PDPTPVPVEAADLQSVMDRDLAGPLKNGALAPGTGVGLAIGVSKHGVRRVFAYGVAKPDSFFEIASITKTFTGLILARMA AEGKVRLDEPVRELLPAGTVPKPRGQEITLLDLATQHSGLPPMPSNFHPADRSNPHADYTVEALYEYIGMHGVAKPEETK FVYSNLGLGLLGQALANRAGVSYRDLLRQEITEPLGLRDTAIALSPEQRQRFLQGTNPEHRPVHEWDVNGLAPAGAIHAT APDMLTYLEANLHPANFPALAQAFAESHRLHAEATGGMQIALAWLYRADSGTYWHNGATAGFTSHAFFDPNHDTAAVVLA NSGPNLLNNPDLIGEHIRQRLAGEPAISLDAAFVPAGSGLSGALRWFFAYWFTMLASGVFIYCGVLGLQGLAAQLLPRRL FLRVSGLLQLTAFCLFVSVYFLQPVFSGMDSLLEPATRRLLPWLPTYWFLGLFHSLNGSMHPALAMLARRAWMGLGIVLC GTAAAYALSYLRTLRKIVEEADITPGARGWNWLPHFGSRVQTAVGQFGVRTLARSRQHRVILAFYFGIGFALTIFLLKAP LKAPVPGAASDPWHQVNPEVLAASIAMMTLGIVGTRVVFAMPLELRANWIFRVTGVRGGSQVLAASRRSLVMLSAGPVWV ATAVLCFSLWPWRQAAGHVVLLGLIGILLTDLCLYGFRKIPFTCSYLPGKSQVHMVFLGALGLLYFIMLSVKFEREMLLQ ARSTAAMLAPFALAAIGFRVWTTLTGAGEDELQFEEAREPAVLELGLHRDGVMPLGPPPVPAAWRCGVNEPRP
Sequences:
>Translated_953_residues MNKQQFRVLYREFLFRMVDLEVLSAHAQGDSHRLLGQFAALLLFISNLLSIAAFGFAGLRMPPAALLGFTMVTEHFLIAT TMLVVGLFAVLSWDSTFPDRRDVLVLAPLPVRARTMFLAKVAAVATALTLTVALLHGASGLIWPLAFASQATAQTVPALT PDPTPVPVEAADLQSVMDRDLAGPLKNGALAPGTGVGLAIGVSKHGVRRVFAYGVAKPDSFFEIASITKTFTGLILARMA AEGKVRLDEPVRELLPAGTVPKPRGQEITLLDLATQHSGLPPMPSNFHPADRSNPHADYTVEALYEYIGMHGVAKPEETK FVYSNLGLGLLGQALANRAGVSYRDLLRQEITEPLGLRDTAIALSPEQRQRFLQGTNPEHRPVHEWDVNGLAPAGAIHAT APDMLTYLEANLHPANFPALAQAFAESHRLHAEATGGMQIALAWLYRADSGTYWHNGATAGFTSHAFFDPNHDTAAVVLA NSGPNLLNNPDLIGEHIRQRLAGEPAISLDAAFVPAGSGLSGALRWFFAYWFTMLASGVFIYCGVLGLQGLAAQLLPRRL FLRVSGLLQLTAFCLFVSVYFLQPVFSGMDSLLEPATRRLLPWLPTYWFLGLFHSLNGSMHPALAMLARRAWMGLGIVLC GTAAAYALSYLRTLRKIVEEADITPGARGWNWLPHFGSRVQTAVGQFGVRTLARSRQHRVILAFYFGIGFALTIFLLKAP LKAPVPGAASDPWHQVNPEVLAASIAMMTLGIVGTRVVFAMPLELRANWIFRVTGVRGGSQVLAASRRSLVMLSAGPVWV ATAVLCFSLWPWRQAAGHVVLLGLIGILLTDLCLYGFRKIPFTCSYLPGKSQVHMVFLGALGLLYFIMLSVKFEREMLLQ ARSTAAMLAPFALAAIGFRVWTTLTGAGEDELQFEEAREPAVLELGLHRDGVMPLGPPPVPAAWRCGVNEPRP >Mature_953_residues MNKQQFRVLYREFLFRMVDLEVLSAHAQGDSHRLLGQFAALLLFISNLLSIAAFGFAGLRMPPAALLGFTMVTEHFLIAT TMLVVGLFAVLSWDSTFPDRRDVLVLAPLPVRARTMFLAKVAAVATALTLTVALLHGASGLIWPLAFASQATAQTVPALT PDPTPVPVEAADLQSVMDRDLAGPLKNGALAPGTGVGLAIGVSKHGVRRVFAYGVAKPDSFFEIASITKTFTGLILARMA AEGKVRLDEPVRELLPAGTVPKPRGQEITLLDLATQHSGLPPMPSNFHPADRSNPHADYTVEALYEYIGMHGVAKPEETK FVYSNLGLGLLGQALANRAGVSYRDLLRQEITEPLGLRDTAIALSPEQRQRFLQGTNPEHRPVHEWDVNGLAPAGAIHAT APDMLTYLEANLHPANFPALAQAFAESHRLHAEATGGMQIALAWLYRADSGTYWHNGATAGFTSHAFFDPNHDTAAVVLA NSGPNLLNNPDLIGEHIRQRLAGEPAISLDAAFVPAGSGLSGALRWFFAYWFTMLASGVFIYCGVLGLQGLAAQLLPRRL FLRVSGLLQLTAFCLFVSVYFLQPVFSGMDSLLEPATRRLLPWLPTYWFLGLFHSLNGSMHPALAMLARRAWMGLGIVLC GTAAAYALSYLRTLRKIVEEADITPGARGWNWLPHFGSRVQTAVGQFGVRTLARSRQHRVILAFYFGIGFALTIFLLKAP LKAPVPGAASDPWHQVNPEVLAASIAMMTLGIVGTRVVFAMPLELRANWIFRVTGVRGGSQVLAASRRSLVMLSAGPVWV ATAVLCFSLWPWRQAAGHVVLLGLIGILLTDLCLYGFRKIPFTCSYLPGKSQVHMVFLGALGLLYFIMLSVKFEREMLLQ ARSTAAMLAPFALAAIGFRVWTTLTGAGEDELQFEEAREPAVLELGLHRDGVMPLGPPPVPAAWRCGVNEPRP
Specific function: Binds penicillin and contributes to the normal morphology [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the beta-lactamase family [H]
Homologues:
Organism=Escherichia coli, GI1786574, Length=356, Percent_Identity=27.8089887640449, Blast_Score=103, Evalue=7e-23, Organism=Escherichia coli, GI1790593, Length=333, Percent_Identity=25.5255255255255, Blast_Score=78, Evalue=2e-15,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001466 [H]
Pfam domain/function: PF00144 Beta-lactamase [H]
EC number: NA
Molecular weight: Translated: 103401; Mature: 103401
Theoretical pI: Translated: 9.17; Mature: 9.17
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNKQQFRVLYREFLFRMVDLEVLSAHAQGDSHRLLGQFAALLLFISNLLSIAAFGFAGLR CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC MPPAALLGFTMVTEHFLIATTMLVVGLFAVLSWDSTFPDRRDVLVLAPLPVRARTMFLAK CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHH VAAVATALTLTVALLHGASGLIWPLAFASQATAQTVPALTPDPTPVPVEAADLQSVMDRD HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH LAGPLKNGALAPGTGVGLAIGVSKHGVRRVFAYGVAKPDSFFEIASITKTFTGLILARMA HCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH AEGKVRLDEPVRELLPAGTVPKPRGQEITLLDLATQHSGLPPMPSNFHPADRSNPHADYT CCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHH VEALYEYIGMHGVAKPEETKFVYSNLGLGLLGQALANRAGVSYRDLLRQEITEPLGLRDT HHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC AIALSPEQRQRFLQGTNPEHRPVHEWDVNGLAPAGAIHATAPDMLTYLEANLHPANFPAL EEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHH AQAFAESHRLHAEATGGMQIALAWLYRADSGTYWHNGATAGFTSHAFFDPNHDTAAVVLA HHHHHHHHCEEEECCCCCEEEEEEEEECCCCCEECCCCCCCCCCCCEECCCCCCEEEEEE NSGPNLLNNPDLIGEHIRQRLAGEPAISLDAAFVPAGSGLSGALRWFFAYWFTMLASGVF CCCCCCCCCCHHHHHHHHHHHCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHH IYCGVLGLQGLAAQLLPRRLFLRVSGLLQLTAFCLFVSVYFLQPVFSGMDSLLEPATRRL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LPWLPTYWFLGLFHSLNGSMHPALAMLARRAWMGLGIVLCGTAAAYALSYLRTLRKIVEE HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ADITPGARGWNWLPHFGSRVQTAVGQFGVRTLARSRQHRVILAFYFGIGFALTIFLLKAP CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCC LKAPVPGAASDPWHQVNPEVLAASIAMMTLGIVGTRVVFAMPLELRANWIFRVTGVRGGS CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCH QVLAASRRSLVMLSAGPVWVATAVLCFSLWPWRQAAGHVVLLGLIGILLTDLCLYGFRKI HHHHHCCCCEEEEECCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCC PFTCSYLPGKSQVHMVFLGALGLLYFIMLSVKFEREMLLQARSTAAMLAPFALAAIGFRV CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH WTTLTGAGEDELQFEEAREPAVLELGLHRDGVMPLGPPPVPAAWRCGVNEPRP HHHCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCC >Mature Secondary Structure MNKQQFRVLYREFLFRMVDLEVLSAHAQGDSHRLLGQFAALLLFISNLLSIAAFGFAGLR CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC MPPAALLGFTMVTEHFLIATTMLVVGLFAVLSWDSTFPDRRDVLVLAPLPVRARTMFLAK CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHH VAAVATALTLTVALLHGASGLIWPLAFASQATAQTVPALTPDPTPVPVEAADLQSVMDRD HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH LAGPLKNGALAPGTGVGLAIGVSKHGVRRVFAYGVAKPDSFFEIASITKTFTGLILARMA HCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH AEGKVRLDEPVRELLPAGTVPKPRGQEITLLDLATQHSGLPPMPSNFHPADRSNPHADYT CCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCHH VEALYEYIGMHGVAKPEETKFVYSNLGLGLLGQALANRAGVSYRDLLRQEITEPLGLRDT HHHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCC AIALSPEQRQRFLQGTNPEHRPVHEWDVNGLAPAGAIHATAPDMLTYLEANLHPANFPAL EEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHH AQAFAESHRLHAEATGGMQIALAWLYRADSGTYWHNGATAGFTSHAFFDPNHDTAAVVLA HHHHHHHHCEEEECCCCCEEEEEEEEECCCCCEECCCCCCCCCCCCEECCCCCCEEEEEE NSGPNLLNNPDLIGEHIRQRLAGEPAISLDAAFVPAGSGLSGALRWFFAYWFTMLASGVF CCCCCCCCCCHHHHHHHHHHHCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHHHHHHHH IYCGVLGLQGLAAQLLPRRLFLRVSGLLQLTAFCLFVSVYFLQPVFSGMDSLLEPATRRL HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH LPWLPTYWFLGLFHSLNGSMHPALAMLARRAWMGLGIVLCGTAAAYALSYLRTLRKIVEE HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH ADITPGARGWNWLPHFGSRVQTAVGQFGVRTLARSRQHRVILAFYFGIGFALTIFLLKAP CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCC LKAPVPGAASDPWHQVNPEVLAASIAMMTLGIVGTRVVFAMPLELRANWIFRVTGVRGGS CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCH QVLAASRRSLVMLSAGPVWVATAVLCFSLWPWRQAAGHVVLLGLIGILLTDLCLYGFRKI HHHHHCCCCEEEEECCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCC PFTCSYLPGKSQVHMVFLGALGLLYFIMLSVKFEREMLLQARSTAAMLAPFALAAIGFRV CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH WTTLTGAGEDELQFEEAREPAVLELGLHRDGVMPLGPPPVPAAWRCGVNEPRP HHHCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEECCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796 [H]