Click here to switch to the map view.

The map label for this gene is rhdA [H]

Identifier: 116624706

GI number: 116624706

Start: 7058366

End: 7059259

Strand: Reverse

Name: rhdA [H]

Synonym: Acid_5630

Alternate gene names: 116624706

Gene position: 7059259-7058366 (Counterclockwise)

Preceding gene: 116624707

Following gene: 116624705

Centisome position: 70.84

GC content: 64.43

Gene sequence:

>894_bases
TTGATACGCAAATTCGCACTGCTCGCCCTTTTCTTCGCCGCGCTTGCCGGTGCCGCAACCTGCGGCGGGCACGGCACGCC
CGAAACTATGATCGTGTCGCCGAAATGGCTGGCCGCGCACCTGGCGGATCCCAACCTCGTTATCCTCGCCGTGGGCGAAC
AGAACGACTACGATGCGGGCCACATCCCCAGTTCGCAGTTCATCGTCTACAAAGACATCGGCGAGGCAGGCCCCACCGGC
CTGATCCTCGAACTGCCGCCGATGCCGCGCCTCGCCGAGGTCTTCGGCAAGTACGGCGTCAGCAACAGTTCGCGTGTGGT
GGTCTATCGCCTCAAGGACTGGCTCACTCCCATCGCCCGCGTGCTCATGACGCTCGACGCCATGGGCCTCGGCAGGAACG
CCGCCATGCTCGATGGAAACCTCGAGATCTGGAAGGAGGAAGGCGGCGCCGTCACCACCGACGTGCCCACCGTGAAGCCT
GGCAAGATCGAGCCCTGCCCGCAGGACGACGTGATCGCCACTCTCGCCTTCGTGCGCGACAATCTCCACTCGCCCGGTTT
CCGCATCCTCGACGCGCGCTCGCCTGAAGCCTACAACGGCGACCCCAAGAGCGTGCGCCCGGGAATCCGTGCCGGCCACA
TCGAAGGAGCCGGCAACGTGCACTACGATAGCCTGCTCACGCCCAAAGGCAAGCTCCTGCCGGCGGCTGACTTACAGGCT
AAGTTCACTGCCGCCGGCGTGAAGCCCGGCGACCGCGTGGTCACGTACTGCTTCATCGGCCAGCAGGCCAGCGCGCTGTA
CTGGTTCTCGCGCTACCTGGGCTACGACACGCGCCTCTACGATGGCTCCATGGACGAATGGAGCAAGCACGACGAATTGC
CGATCGTAAAGTAG

Upstream 100 bases:

>100_bases
CGTTTGCGGGTGCGCGCAATGGCCGCTACGCCGCGATCACGGCTGCCGTGCTCGCCGTACTGGGTTACAGTATGTTCGGT
ACGCACTGAGGAGACAAATC

Downstream 100 bases:

>100_bases
TCTGCGCCTCCCCTTCGCTAAACTAGAGGAATGAGATCCGCTGCCCTGCTTGTGTTCGCAGCGTCCGCGCTCGCGGCCCA
GTCGCCCTGCGCCAATACGC

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MIRKFALLALFFAALAGAATCGGHGTPETMIVSPKWLAAHLADPNLVILAVGEQNDYDAGHIPSSQFIVYKDIGEAGPTG
LILELPPMPRLAEVFGKYGVSNSSRVVVYRLKDWLTPIARVLMTLDAMGLGRNAAMLDGNLEIWKEEGGAVTTDVPTVKP
GKIEPCPQDDVIATLAFVRDNLHSPGFRILDARSPEAYNGDPKSVRPGIRAGHIEGAGNVHYDSLLTPKGKLLPAADLQA
KFTAAGVKPGDRVVTYCFIGQQASALYWFSRYLGYDTRLYDGSMDEWSKHDELPIVK

Sequences:

>Translated_297_residues
MIRKFALLALFFAALAGAATCGGHGTPETMIVSPKWLAAHLADPNLVILAVGEQNDYDAGHIPSSQFIVYKDIGEAGPTG
LILELPPMPRLAEVFGKYGVSNSSRVVVYRLKDWLTPIARVLMTLDAMGLGRNAAMLDGNLEIWKEEGGAVTTDVPTVKP
GKIEPCPQDDVIATLAFVRDNLHSPGFRILDARSPEAYNGDPKSVRPGIRAGHIEGAGNVHYDSLLTPKGKLLPAADLQA
KFTAAGVKPGDRVVTYCFIGQQASALYWFSRYLGYDTRLYDGSMDEWSKHDELPIVK
>Mature_297_residues
MIRKFALLALFFAALAGAATCGGHGTPETMIVSPKWLAAHLADPNLVILAVGEQNDYDAGHIPSSQFIVYKDIGEAGPTG
LILELPPMPRLAEVFGKYGVSNSSRVVVYRLKDWLTPIARVLMTLDAMGLGRNAAMLDGNLEIWKEEGGAVTTDVPTVKP
GKIEPCPQDDVIATLAFVRDNLHSPGFRILDARSPEAYNGDPKSVRPGIRAGHIEGAGNVHYDSLLTPKGKLLPAADLQA
KFTAAGVKPGDRVVTYCFIGQQASALYWFSRYLGYDTRLYDGSMDEWSKHDELPIVK

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=278, Percent_Identity=28.7769784172662, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI194473668, Length=292, Percent_Identity=25, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI61835204, Length=274, Percent_Identity=25.1824817518248, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI194473681, Length=274, Percent_Identity=25.1824817518248, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87082121, Length=279, Percent_Identity=25.4480286738351, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87081967, Length=292, Percent_Identity=25, Blast_Score=74, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17561888, Length=318, Percent_Identity=24.2138364779874, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71997283, Length=206, Percent_Identity=29.6116504854369, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 32178; Mature: 32178

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50206 RHODANESE_3 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKFALLALFFAALAGAATCGGHGTPETMIVSPKWLAAHLADPNLVILAVGEQNDYDAG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHEEECCCCCEEEEEECCCCCCCCC
HIPSSQFIVYKDIGEAGPTGLILELPPMPRLAEVFGKYGVSNSSRVVVYRLKDWLTPIAR
CCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
VLMTLDAMGLGRNAAMLDGNLEIWKEEGGAVTTDVPTVKPGKIEPCPQDDVIATLAFVRD
HHHHHHHHCCCCCCEEECCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
NLHSPGFRILDARSPEAYNGDPKSVRPGIRAGHIEGAGNVHYDSLLTPKGKLLPAADLQA
CCCCCCEEEEECCCCCCCCCCCHHCCCCCEECCCCCCCCEEECCEECCCCCCCCCCCCCE
KFTAAGVKPGDRVVTYCFIGQQASALYWFSRYLGYDTRLYDGSMDEWSKHDELPIVK
EEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCC
>Mature Secondary Structure
MIRKFALLALFFAALAGAATCGGHGTPETMIVSPKWLAAHLADPNLVILAVGEQNDYDAG
CCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCHHHEEECCCCCEEEEEECCCCCCCCC
HIPSSQFIVYKDIGEAGPTGLILELPPMPRLAEVFGKYGVSNSSRVVVYRLKDWLTPIAR
CCCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHH
VLMTLDAMGLGRNAAMLDGNLEIWKEEGGAVTTDVPTVKPGKIEPCPQDDVIATLAFVRD
HHHHHHHHCCCCCCEEECCCEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
NLHSPGFRILDARSPEAYNGDPKSVRPGIRAGHIEGAGNVHYDSLLTPKGKLLPAADLQA
CCCCCCEEEEECCCCCCCCCCCHHCCCCCEECCCCCCCCEEECCEECCCCCCCCCCCCCE
KFTAAGVKPGDRVVTYCFIGQQASALYWFSRYLGYDTRLYDGSMDEWSKHDELPIVK
EEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8617271; 10788330 [H]