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The map label for this gene is dgoD [H]

Identifier: 116624699

GI number: 116624699

Start: 7048893

End: 7050128

Strand: Direct

Name: dgoD [H]

Synonym: Acid_5623

Alternate gene names: 116624699

Gene position: 7048893-7050128 (Clockwise)

Preceding gene: 116624698

Following gene: 116624700

Centisome position: 70.73

GC content: 64.08

Gene sequence:

>1236_bases
GTGAAGCGTCGTCTGTTTCTCGGAGCCGCCCTTGGCGGACTCGCAGGGTCTCTGTTCGACCCGATCCGCGGGTTCGCGCA
GGACGCGTCGGCGCGCGCGAAGCCGCTGAACCTGAAGATCATGGGACTGAAGACCTTCGTGGTCAATGCGGGCAGCCTGA
ACTGGGTCTTCTGCAAGGTCTATACCAACGCGGGCCTGATCGGGCTGGGCGAAGGCTCGGTGACCAGCAAGGAGGCCACG
ATTGCGGCGGCGATCCAGGAGCACGAGCGCTTCCTGGTTGGCAAGGACCCGACCGACATCGAACTGCTCTGGCAGGGCAT
GTACCGGTTTCCCCGATGGCGCGGCGGTCCGATTCTGAATTCCGCGATCAGCGCGGTGGAGATCGCGCTCTGGGACATTC
TCGGCCAGGCGCTGGGTGTGCCGATTTATAAGCTGTTGGGCGGCGCGGCGCGTTCCCGCGTGCGCATGTACGTAGACGTC
GGTTCGACGCCCGAAGAGTTTCTGCGCGCCAGGGAGCTGGGCTATACCGCGGCCAAGGCGACGCCGCTCTCTCCGGATCA
GAACCTGGTGTCGCCGACCAAGATGATTCGCGATGGCGTCAGTAAACTGCAGGCGATCCGCAAGGCCGTGGGAGACGATT
TCGATATCGCGGTGGATGCGCACGGGCGCTGCACCACCACGATGGCAATCGATTTCTGCACGCGCGTCGAAGACCTGGAC
CTGCTCTTCGTGGAGGAACCCACGCAGTTGGAGGATCTCGGCGAATTGGCGCTGCTACGCGAGAAGACCAAAACCCACCT
CGCCACCGGCGAGCGCTCGTTCACCAAGTACGGGTTCGCGGATTTCTGTTCGCGACACCTGGTGGATTACATCCAGCCGG
ATGTCTGCCACGCCGGCGGCATCCTGGAATTGAAGAAGATCGGCGTGCTGGCGGAGACGTACCGCGTCAACCTGGCGCCG
CACAATCCGCAGAGCTTGGTGAGCACCATGGCTTCGCTGCACGTGGACGCCACCACGCCCAGCGCCGCGATTCAGGAGTC
CACGCTCAACCGTACGGCCTGGCTGCAGGATCTGTTCGAGGGGACCGGGCCGGAAGTGAAGCAGGGCTACGCCGAACTGC
CCACGCGCCCCGGACTGGGAGTGACCCTCAACGAAAAGGTCGCGGCCGCGCATCCCTACAAACCGACCAACCGCCCGGAA
TACCGGTTCGGGGACGGCTCGGTGACCGATCAGTAG

Upstream 100 bases:

>100_bases
GTGACGGTTGCCTGCCTCAGCAGGAGCCGTCACATGGCGGCCCTTTCGCGAATTACAATCCCCGCGTTACAATTCCAAAC
ACTGACAGGAGGACACGCCT

Downstream 100 bases:

>100_bases
GAGCGCTTGCCAAACAGGCCGCGACTTAACTATAGTTAAGTACATGTTGCCGACGAGTCGTACGGCGGAGGAATCGCGGT
CCCTGGCGGACGTGCACTCA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKRRLFLGAALGGLAGSLFDPIRGFAQDASARAKPLNLKIMGLKTFVVNAGSLNWVFCKVYTNAGLIGLGEGSVTSKEAT
IAAAIQEHERFLVGKDPTDIELLWQGMYRFPRWRGGPILNSAISAVEIALWDILGQALGVPIYKLLGGAARSRVRMYVDV
GSTPEEFLRARELGYTAAKATPLSPDQNLVSPTKMIRDGVSKLQAIRKAVGDDFDIAVDAHGRCTTTMAIDFCTRVEDLD
LLFVEEPTQLEDLGELALLREKTKTHLATGERSFTKYGFADFCSRHLVDYIQPDVCHAGGILELKKIGVLAETYRVNLAP
HNPQSLVSTMASLHVDATTPSAAIQESTLNRTAWLQDLFEGTGPEVKQGYAELPTRPGLGVTLNEKVAAAHPYKPTNRPE
YRFGDGSVTDQ

Sequences:

>Translated_411_residues
MKRRLFLGAALGGLAGSLFDPIRGFAQDASARAKPLNLKIMGLKTFVVNAGSLNWVFCKVYTNAGLIGLGEGSVTSKEAT
IAAAIQEHERFLVGKDPTDIELLWQGMYRFPRWRGGPILNSAISAVEIALWDILGQALGVPIYKLLGGAARSRVRMYVDV
GSTPEEFLRARELGYTAAKATPLSPDQNLVSPTKMIRDGVSKLQAIRKAVGDDFDIAVDAHGRCTTTMAIDFCTRVEDLD
LLFVEEPTQLEDLGELALLREKTKTHLATGERSFTKYGFADFCSRHLVDYIQPDVCHAGGILELKKIGVLAETYRVNLAP
HNPQSLVSTMASLHVDATTPSAAIQESTLNRTAWLQDLFEGTGPEVKQGYAELPTRPGLGVTLNEKVAAAHPYKPTNRPE
YRFGDGSVTDQ
>Mature_411_residues
MKRRLFLGAALGGLAGSLFDPIRGFAQDASARAKPLNLKIMGLKTFVVNAGSLNWVFCKVYTNAGLIGLGEGSVTSKEAT
IAAAIQEHERFLVGKDPTDIELLWQGMYRFPRWRGGPILNSAISAVEIALWDILGQALGVPIYKLLGGAARSRVRMYVDV
GSTPEEFLRARELGYTAAKATPLSPDQNLVSPTKMIRDGVSKLQAIRKAVGDDFDIAVDAHGRCTTTMAIDFCTRVEDLD
LLFVEEPTQLEDLGELALLREKTKTHLATGERSFTKYGFADFCSRHLVDYIQPDVCHAGGILELKKIGVLAETYRVNLAP
HNPQSLVSTMASLHVDATTPSAAIQESTLNRTAWLQDLFEGTGPEVKQGYAELPTRPGLGVTLNEKVAAAHPYKPTNRPE
YRFGDGSVTDQ

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=368, Percent_Identity=34.7826086956522, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1787864, Length=406, Percent_Identity=31.0344827586207, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI226510960, Length=327, Percent_Identity=27.5229357798165, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44791; Mature: 44791

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRLFLGAALGGLAGSLFDPIRGFAQDASARAKPLNLKIMGLKTFVVNAGSLNWVFCKV
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEEE
YTNAGLIGLGEGSVTSKEATIAAAIQEHERFLVGKDPTDIELLWQGMYRFPRWRGGPILN
ECCCCEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCHHH
SAISAVEIALWDILGQALGVPIYKLLGGAARSRVRMYVDVGSTPEEFLRARELGYTAAKA
HHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHCEEEEEEECCCCHHHHHHHHHCCCHHHCC
TPLSPDQNLVSPTKMIRDGVSKLQAIRKAVGDDFDIAVDAHGRCTTTMAIDFCTRVEDLD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHCCCCCE
LLFVEEPTQLEDLGELALLREKTKTHLATGERSFTKYGFADFCSRHLVDYIQPDVCHAGG
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCHHCCCC
ILELKKIGVLAETYRVNLAPHNPQSLVSTMASLHVDATTPSAAIQESTLNRTAWLQDLFE
EEEHHHHHHEEEEEEEEECCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHH
GTGPEVKQGYAELPTRPGLGVTLNEKVAAAHPYKPTNRPEYRFGDGSVTDQ
CCCCHHHHHHHHCCCCCCCCEEECCCHHCCCCCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MKRRLFLGAALGGLAGSLFDPIRGFAQDASARAKPLNLKIMGLKTFVVNAGSLNWVFCKV
CCCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEEECCCCEEEEEEE
YTNAGLIGLGEGSVTSKEATIAAAIQEHERFLVGKDPTDIELLWQGMYRFPRWRGGPILN
ECCCCEEECCCCCCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCHHH
SAISAVEIALWDILGQALGVPIYKLLGGAARSRVRMYVDVGSTPEEFLRARELGYTAAKA
HHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHCEEEEEEECCCCHHHHHHHHHCCCHHHCC
TPLSPDQNLVSPTKMIRDGVSKLQAIRKAVGDDFDIAVDAHGRCTTTMAIDFCTRVEDLD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEHHHHHHHHHCCCCCE
LLFVEEPTQLEDLGELALLREKTKTHLATGERSFTKYGFADFCSRHLVDYIQPDVCHAGG
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHCCCCHHCCCC
ILELKKIGVLAETYRVNLAPHNPQSLVSTMASLHVDATTPSAAIQESTLNRTAWLQDLFE
EEEHHHHHHEEEEEEEEECCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHH
GTGPEVKQGYAELPTRPGLGVTLNEKVAAAHPYKPTNRPEYRFGDGSVTDQ
CCCCHHHHHHHHCCCCCCCCEEECCCHHCCCCCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA