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The map label for this gene is amtB [C]

Identifier: 116624608

GI number: 116624608

Start: 6936623

End: 6938269

Strand: Reverse

Name: amtB [C]

Synonym: Acid_5532

Alternate gene names: 116624608

Gene position: 6938269-6936623 (Counterclockwise)

Preceding gene: 116624609

Following gene: 116624607

Centisome position: 69.62

GC content: 59.74

Gene sequence:

>1647_bases
GTGAAAAGAACCCTATTATTATTCATCCCCCTGATTCTCAGCGCCTCGCTTGCGTTAGCCCAGGCGCCGGCAGCCGCTTC
TCCACCGGCCCCCGCAGCGCCGCCGGTGGCGATCACGGATGCGGACGTCAAAAATGTGGACGCACCCAAGCCCGACGTGC
GGCTTAAAGGCGACCCTGACGGCGCACTAACGGGCACTGTATCGGATGTCGCGGTGAGCGATGCAAAGAAAGGCCTCACA
ATCGGCGACCTGGTGAATCAGGTTGGCCAGAACAAGGTTGCGATCAACTTCACGTGGACGCTGATCACCGGCTTCCTGGT
GATGTTCATGCAAGCCGGCTTCGCGATTGTGGAGACGGGCCTTTGCCGAGCGAAGAACGCCAACCACACGATGATGATGA
ATTTCATGGTGTATGGCATCGGCATGCTGGCATACTGGCTGATCGGATTTAGCATCCAGATGGGCGGCGTGGGCGGTGTT
GGCAATCTTGGCGGAGCGCCTCCCCTGAATACCGAGCACGTCATTACGCTCTTCGGCAAAGGTTTCGGCTTGTATGGGCA
GCACGGAATGTTTCTCACGCAAGGCGGCACCTATGACGTCGCCGTGATGGTCATCTTCCTCTTCCAGATGGTCTTCATGG
ATACCGCGCTGACCATTGTCACCGGTTCCGCGGCCGAGCGTTGGAAATTCGCGGCATTCCTTGTATCGTCCTTCATGCTG
GGCGCGTTTATTTACCCGCTGTTCGGCAACTGGGCCTGGGGCGGCGGCTGGCTGGCGACGCTGGGCACCAACTTCGGTCT
GGGCAAGGGCTATTGCGATTTCGCCGGTTCCGGAGTGGTGCATGCGGTGGGAGGCTTGACGGCCCTGGCGCTGTCGATCA
TAGTCGGCCCACGTCTGGGAAAATACAATCGCAACGGCAAGCCGAATGCGATCGTGGGCCACGATATCGTGCTCGTGCTC
ACCGGCTGCTTCATCCTGGCGTTCGGATGGTTCGGTTTCAATCCCGGTTCCACGCTGGGCGCCTCCGGGAGCGGTAGCCT
GAGGATTGGGTCAATCGCGGTGAATACCATGCTGGCCGGGATGGCCGGCGGATTCGGCGCAATGTTCTACATGTGGATGC
GCTACGGCAAGCCAGACGCATCCATGTCGGGCAACGGGCTGCTGGCCGGCCTGGTAGCGATCACGGCGCCCAGCGGGTTC
GTCAATACCGTTGGCGCGGTGATCATCGGATTAATCGCGGGTGTTCTGGTATGCCTGTCGGTGGAATTCATCGACCGGGT
AATGAGGGTCGATGATCCGGTCGGTGCGATTTCGGTGCACGGCGTCAATGGAATCTGGGGTGTAATCGCAGTCGGACTAT
TCGCCGACGGCAAGAGCAACTACGGCGGCTCCTGGAACGGCGTTAACGGTTCCGTGACCGGCTTGTTTTACGGCGATCCG
AGCCAGTTGGTGGCGCAATTGATCGGCGTCTCGACTTTGGTCGGCGGGGTCTTTGTGCTCAGCTTCGTGGTCAACCTGGC
GATTGATGCTGTGGTAGGCCAGAGAGTCTCGGCGAAGGCCGAGCTCGAAGGGCTCGATCTGCCCGAAATGGGTCAAGTCG
GTTACCCCGAATTCGAGCTGAAGGCCAGAACGGTAGGAGTCCAGTAA

Upstream 100 bases:

>100_bases
TGGTTATGGTGTTGATCGGACTGGCGGTAAGCCTCACCGGGATTATTGGAATCATCAATCGCGCGTACCTGAAGAATGCC
AATTGGAGGAGATAACGTCC

Downstream 100 bases:

>100_bases
CATGACGGGAGAACTGGCAGTCACGCAAAACACAGCTTTGCGTTTGCCTCCGGTGCCATGGCGAGACCCTCACACGGTCT
CGCCTGCCCAACTGGCGGAA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKRTLLLFIPLILSASLALAQAPAAASPPAPAAPPVAITDADVKNVDAPKPDVRLKGDPDGALTGTVSDVAVSDAKKGLT
IGDLVNQVGQNKVAINFTWTLITGFLVMFMQAGFAIVETGLCRAKNANHTMMMNFMVYGIGMLAYWLIGFSIQMGGVGGV
GNLGGAPPLNTEHVITLFGKGFGLYGQHGMFLTQGGTYDVAVMVIFLFQMVFMDTALTIVTGSAAERWKFAAFLVSSFML
GAFIYPLFGNWAWGGGWLATLGTNFGLGKGYCDFAGSGVVHAVGGLTALALSIIVGPRLGKYNRNGKPNAIVGHDIVLVL
TGCFILAFGWFGFNPGSTLGASGSGSLRIGSIAVNTMLAGMAGGFGAMFYMWMRYGKPDASMSGNGLLAGLVAITAPSGF
VNTVGAVIIGLIAGVLVCLSVEFIDRVMRVDDPVGAISVHGVNGIWGVIAVGLFADGKSNYGGSWNGVNGSVTGLFYGDP
SQLVAQLIGVSTLVGGVFVLSFVVNLAIDAVVGQRVSAKAELEGLDLPEMGQVGYPEFELKARTVGVQ

Sequences:

>Translated_548_residues
MKRTLLLFIPLILSASLALAQAPAAASPPAPAAPPVAITDADVKNVDAPKPDVRLKGDPDGALTGTVSDVAVSDAKKGLT
IGDLVNQVGQNKVAINFTWTLITGFLVMFMQAGFAIVETGLCRAKNANHTMMMNFMVYGIGMLAYWLIGFSIQMGGVGGV
GNLGGAPPLNTEHVITLFGKGFGLYGQHGMFLTQGGTYDVAVMVIFLFQMVFMDTALTIVTGSAAERWKFAAFLVSSFML
GAFIYPLFGNWAWGGGWLATLGTNFGLGKGYCDFAGSGVVHAVGGLTALALSIIVGPRLGKYNRNGKPNAIVGHDIVLVL
TGCFILAFGWFGFNPGSTLGASGSGSLRIGSIAVNTMLAGMAGGFGAMFYMWMRYGKPDASMSGNGLLAGLVAITAPSGF
VNTVGAVIIGLIAGVLVCLSVEFIDRVMRVDDPVGAISVHGVNGIWGVIAVGLFADGKSNYGGSWNGVNGSVTGLFYGDP
SQLVAQLIGVSTLVGGVFVLSFVVNLAIDAVVGQRVSAKAELEGLDLPEMGQVGYPEFELKARTVGVQ
>Mature_548_residues
MKRTLLLFIPLILSASLALAQAPAAASPPAPAAPPVAITDADVKNVDAPKPDVRLKGDPDGALTGTVSDVAVSDAKKGLT
IGDLVNQVGQNKVAINFTWTLITGFLVMFMQAGFAIVETGLCRAKNANHTMMMNFMVYGIGMLAYWLIGFSIQMGGVGGV
GNLGGAPPLNTEHVITLFGKGFGLYGQHGMFLTQGGTYDVAVMVIFLFQMVFMDTALTIVTGSAAERWKFAAFLVSSFML
GAFIYPLFGNWAWGGGWLATLGTNFGLGKGYCDFAGSGVVHAVGGLTALALSIIVGPRLGKYNRNGKPNAIVGHDIVLVL
TGCFILAFGWFGFNPGSTLGASGSGSLRIGSIAVNTMLAGMAGGFGAMFYMWMRYGKPDASMSGNGLLAGLVAITAPSGF
VNTVGAVIIGLIAGVLVCLSVEFIDRVMRVDDPVGAISVHGVNGIWGVIAVGLFADGKSNYGGSWNGVNGSVTGLFYGDP
SQLVAQLIGVSTLVGGVFVLSFVVNLAIDAVVGQRVSAKAELEGLDLPEMGQVGYPEFELKARTVGVQ

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=437, Percent_Identity=31.1212814645309, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17550180, Length=443, Percent_Identity=30.4740406320542, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=30.3854875283447, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI32564042, Length=451, Percent_Identity=29.9334811529933, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI133931100, Length=448, Percent_Identity=26.3392857142857, Blast_Score=126, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325396, Length=447, Percent_Identity=27.7404921700224, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321559, Length=446, Percent_Identity=26.9058295964126, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324187, Length=452, Percent_Identity=26.1061946902655, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI161078314, Length=449, Percent_Identity=35.4120267260579, Blast_Score=185, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 56754; Mature: 56754

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTLLLFIPLILSASLALAQAPAAASPPAPAAPPVAITDADVKNVDAPKPDVRLKGDPD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCC
GALTGTVSDVAVSDAKKGLTIGDLVNQVGQNKVAINFTWTLITGFLVMFMQAGFAIVETG
CCEECCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHC
LCRAKNANHTMMMNFMVYGIGMLAYWLIGFSIQMGGVGGVGNLGGAPPLNTEHVITLFGK
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEEEEC
GFGLYGQHGMFLTQGGTYDVAVMVIFLFQMVFMDTALTIVTGSAAERWKFAAFLVSSFML
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHH
GAFIYPLFGNWAWGGGWLATLGTNFGLGKGYCDFAGSGVVHAVGGLTALALSIIVGPRLG
HHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
KYNRNGKPNAIVGHDIVLVLTGCFILAFGWFGFNPGSTLGASGSGSLRIGSIAVNTMLAG
CCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
MAGGFGAMFYMWMRYGKPDASMSGNGLLAGLVAITAPSGFVNTVGAVIIGLIAGVLVCLS
HCCHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VEFIDRVMRVDDPVGAISVHGVNGIWGVIAVGLFADGKSNYGGSWNGVNGSVTGLFYGDP
HHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCH
SQLVAQLIGVSTLVGGVFVLSFVVNLAIDAVVGQRVSAKAELEGLDLPEMGQVGYPEFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCEEE
KARTVGVQ
EEEEECCC
>Mature Secondary Structure
MKRTLLLFIPLILSASLALAQAPAAASPPAPAAPPVAITDADVKNVDAPKPDVRLKGDPD
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCC
GALTGTVSDVAVSDAKKGLTIGDLVNQVGQNKVAINFTWTLITGFLVMFMQAGFAIVETG
CCEECCHHHHHHHHHHCCCCHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHCCHHHHHHC
LCRAKNANHTMMMNFMVYGIGMLAYWLIGFSIQMGGVGGVGNLGGAPPLNTEHVITLFGK
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCCEEEEEEC
GFGLYGQHGMFLTQGGTYDVAVMVIFLFQMVFMDTALTIVTGSAAERWKFAAFLVSSFML
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHH
GAFIYPLFGNWAWGGGWLATLGTNFGLGKGYCDFAGSGVVHAVGGLTALALSIIVGPRLG
HHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
KYNRNGKPNAIVGHDIVLVLTGCFILAFGWFGFNPGSTLGASGSGSLRIGSIAVNTMLAG
CCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
MAGGFGAMFYMWMRYGKPDASMSGNGLLAGLVAITAPSGFVNTVGAVIIGLIAGVLVCLS
HCCHHHHHHHHHHHHCCCCCCCCCCCHHEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
VEFIDRVMRVDDPVGAISVHGVNGIWGVIAVGLFADGKSNYGGSWNGVNGSVTGLFYGDP
HHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCH
SQLVAQLIGVSTLVGGVFVLSFVVNLAIDAVVGQRVSAKAELEGLDLPEMGQVGYPEFEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCEEE
KARTVGVQ
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]