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The map label for this gene is rsbU [H]

Identifier: 116624432

GI number: 116624432

Start: 6714825

End: 6716054

Strand: Direct

Name: rsbU [H]

Synonym: Acid_5354

Alternate gene names: 116624432

Gene position: 6714825-6716054 (Clockwise)

Preceding gene: 116624424

Following gene: 116624433

Centisome position: 67.38

GC content: 63.17

Gene sequence:

>1230_bases
ATGCGGTTTGCCGAACTTCTTTCGGCACATGCGGATCGCAGCGGCACGAAACGTGCGATATCCGCGTCAGTTAAGATGTG
GAAGACCCTGATGCAACCGACCCCGCAAGCCCAGACGGAAGTCAGACCGTATCGTGTCCTAATCTGCGACGATCAGCCCG
ACGTGTTGGAGGCGCTCCGGCTGCTCCTGAAAGGGCAGGGCTGGCAGGCGGTGACTGCCGATTCGCCGAAGTCCCTGCTT
GAAACCGTGCGCGGCGGCGGCTTCGATCTGATTCTCACCGACCTCAACTACACCCGCGACACGACCTCCGGACAGGAGGG
GATGGACCTGCTGGCCAGTCTGGAAGCGCAGGGCAACGCTGTGCCGGTGGTGGTGATGACCGCCTGGGGCAACGTGGATC
TCGCGGTGGAAGCCATGCGCCGCGGCGCCTGCGATTTCGTGCAGAAGCCGTGGGATAACAATCGCCTGCTGGCCACGATT
CGCAAACAGGCCGAGCCGGGACGCCGCCGCAAGAGCGAACTGGAGATCGCGGCCAATGTTCAGCAGAAGCTTTTCCCGCG
TAAGCTGCGAAGGTTGGAATCGCTGGACTACGCCGGGCATTGCATGCCGGCGCGCGAAGTGGGCGGAGACTACTACGATT
TTCTCGAGATGCCCGATTCCACCATCGGGTTCGTGCTCGGCGATGTTTCGGGGAAAGGCGTTCCGGCTGCGCTGTTGATG
GCGAATTTGCAGGCCTGCTTCCGCAGCCAGCCTCCGCATGCGCTACAGCGGCCCGCCGAAGTGCTGGGCATGGTCAACCG
GCATTTCTTCGATTCGACCGCCGCCGAACGCTTCGCTACCCTGGTCTTCGCCAGCTATGACGATCGGACCCGCAAGGTCC
GCTATGTGAATTGCGCGCACTGTCCGCCGCTGTTGCTGCGCGCCAGTGGCGAACTGGAAATGTTGGATTCCACGGCGACG
ATGCTCGGGGCGTTTACGAAGTGGACGTGCACCGAAGGAGAAACCGCGCTGCGCACCGGCGACACGCTACTGCTCTATTC
CGACGGAGTTACCGAAGCGGGCGCCGACACCGGCGAGGAATTCGGCGAGGAGCGCCTGATTCACGCACTGCGCGTGAATC
AACAGCAACCGGCGGAAGCGCTGGTGCAGGCGATCATCGACGACGTCAACGATTTCAGCGGCGGCGCGCGCAGCGACGAT
GTCACCGTGGTGGCGCTGCGGGGGCTGTAG

Upstream 100 bases:

>100_bases
TTTTAGCTGGTCCATTGGGGGGTTAACTTTATTTTAGCAAGTTGGGTACCTCCTGATAGGCGCAGCCCGGAGGCGGACAG
GGCGTCCCACAACCGCACAC

Downstream 100 bases:

>100_bases
GACGGACGCCTGATTAGTACCAATAATCCTCAAAGTTTGGAGTTACTATGGTGGCGAATGGGAACGCCTATGGATCTCAA
ACTCTTGCAGGACACGCCAC

Product: response regulator receiver modulated serine phosphatase

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MRFAELLSAHADRSGTKRAISASVKMWKTLMQPTPQAQTEVRPYRVLICDDQPDVLEALRLLLKGQGWQAVTADSPKSLL
ETVRGGGFDLILTDLNYTRDTTSGQEGMDLLASLEAQGNAVPVVVMTAWGNVDLAVEAMRRGACDFVQKPWDNNRLLATI
RKQAEPGRRRKSELEIAANVQQKLFPRKLRRLESLDYAGHCMPAREVGGDYYDFLEMPDSTIGFVLGDVSGKGVPAALLM
ANLQACFRSQPPHALQRPAEVLGMVNRHFFDSTAAERFATLVFASYDDRTRKVRYVNCAHCPPLLLRASGELEMLDSTAT
MLGAFTKWTCTEGETALRTGDTLLLYSDGVTEAGADTGEEFGEERLIHALRVNQQQPAEALVQAIIDDVNDFSGGARSDD
VTVVALRGL

Sequences:

>Translated_409_residues
MRFAELLSAHADRSGTKRAISASVKMWKTLMQPTPQAQTEVRPYRVLICDDQPDVLEALRLLLKGQGWQAVTADSPKSLL
ETVRGGGFDLILTDLNYTRDTTSGQEGMDLLASLEAQGNAVPVVVMTAWGNVDLAVEAMRRGACDFVQKPWDNNRLLATI
RKQAEPGRRRKSELEIAANVQQKLFPRKLRRLESLDYAGHCMPAREVGGDYYDFLEMPDSTIGFVLGDVSGKGVPAALLM
ANLQACFRSQPPHALQRPAEVLGMVNRHFFDSTAAERFATLVFASYDDRTRKVRYVNCAHCPPLLLRASGELEMLDSTAT
MLGAFTKWTCTEGETALRTGDTLLLYSDGVTEAGADTGEEFGEERLIHALRVNQQQPAEALVQAIIDDVNDFSGGARSDD
VTVVALRGL
>Mature_409_residues
MRFAELLSAHADRSGTKRAISASVKMWKTLMQPTPQAQTEVRPYRVLICDDQPDVLEALRLLLKGQGWQAVTADSPKSLL
ETVRGGGFDLILTDLNYTRDTTSGQEGMDLLASLEAQGNAVPVVVMTAWGNVDLAVEAMRRGACDFVQKPWDNNRLLATI
RKQAEPGRRRKSELEIAANVQQKLFPRKLRRLESLDYAGHCMPAREVGGDYYDFLEMPDSTIGFVLGDVSGKGVPAALLM
ANLQACFRSQPPHALQRPAEVLGMVNRHFFDSTAAERFATLVFASYDDRTRKVRYVNCAHCPPLLLRASGELEMLDSTAT
MLGAFTKWTCTEGETALRTGDTLLLYSDGVTEAGADTGEEFGEERLIHALRVNQQQPAEALVQAIIDDVNDFSGGARSDD
VTVVALRGL

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=36.7521367521368, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 44965; Mature: 44965

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFAELLSAHADRSGTKRAISASVKMWKTLMQPTPQAQTEVRPYRVLICDDQPDVLEALR
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHH
LLLKGQGWQAVTADSPKSLLETVRGGGFDLILTDLNYTRDTTSGQEGMDLLASLEAQGNA
HHHCCCCCEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCE
VPVVVMTAWGNVDLAVEAMRRGACDFVQKPWDNNRLLATIRKQAEPGRRRKSELEIAANV
EEEEEEEECCCCHHHHHHHHCCCHHHHHCCCCCCCEEHHHHHHCCCCHHHHHHHHHHHHH
QQKLFPRKLRRLESLDYAGHCMPAREVGGDYYDFLEMPDSTIGFVLGDVSGKGVPAALLM
HHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
ANLQACFRSQPPHALQRPAEVLGMVNRHFFDSTAAERFATLVFASYDDRTRKVRYVNCAH
HHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCC
CPPLLLRASGELEMLDSTATMLGAFTKWTCTEGETALRTGDTLLLYSDGVTEAGADTGEE
CCCEEEECCCCCEEHHHHHHHHHHHHEEEECCCCCEEECCCEEEEEECCCCCCCCCCHHH
FGEERLIHALRVNQQQPAEALVQAIIDDVNDFSGGARSDDVTVVALRGL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MRFAELLSAHADRSGTKRAISASVKMWKTLMQPTPQAQTEVRPYRVLICDDQPDVLEALR
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHH
LLLKGQGWQAVTADSPKSLLETVRGGGFDLILTDLNYTRDTTSGQEGMDLLASLEAQGNA
HHHCCCCCEEECCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCE
VPVVVMTAWGNVDLAVEAMRRGACDFVQKPWDNNRLLATIRKQAEPGRRRKSELEIAANV
EEEEEEEECCCCHHHHHHHHCCCHHHHHCCCCCCCEEHHHHHHCCCCHHHHHHHHHHHHH
QQKLFPRKLRRLESLDYAGHCMPAREVGGDYYDFLEMPDSTIGFVLGDVSGKGVPAALLM
HHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
ANLQACFRSQPPHALQRPAEVLGMVNRHFFDSTAAERFATLVFASYDDRTRKVRYVNCAH
HHHHHHHCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEEECCC
CPPLLLRASGELEMLDSTATMLGAFTKWTCTEGETALRTGDTLLLYSDGVTEAGADTGEE
CCCEEEECCCCCEEHHHHHHHHHHHHEEEECCCCCEEECCCEEEEEECCCCCCCCCCHHH
FGEERLIHALRVNQQQPAEALVQAIIDDVNDFSGGARSDDVTVVALRGL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]