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The map label for this gene is gatA [H]

Identifier: 116624411

GI number: 116624411

Start: 6685411

End: 6686601

Strand: Direct

Name: gatA [H]

Synonym: Acid_5333

Alternate gene names: 116624411

Gene position: 6685411-6686601 (Clockwise)

Preceding gene: 116624410

Following gene: 116624412

Centisome position: 67.08

GC content: 68.93

Gene sequence:

>1191_bases
TTGCTGCTGCAATTTCTGCGTTGGCTCGAATCCGGCGAGAAGACGCCGCAAGATCTTCTGGCGCTCTGCCTGAGCCGCGT
CGCGGAGCTGGATGGGGATGTGCGCGCGTGGGTCGTGGTGGGGGCGCAGCCCGCGCTTGCCGAGGGTGCGCTGGCGGGAA
TTCCGTTCGGCGTCAAGGATATCTTCGAGACCAGCGGACTACCTACGGAATTCGGCTCGCCTCTTTACCGGGGACGGCGC
GGCGAATGCGATGCGCAGATCGTGGCGCACCTGCGTCTCGCGGGCGCGGTGCTGCTCGGCAAGACCGAGACCACGCCATT
CGCGTCCTTCGATGCGGCTCCGACACGCAATCCGAGGCTTCCCGGCCACACGCCGGGCGGCAGTTCGGCTGGCTCGGCGG
CGGCGGTTGCCGCCGGAATGGTCCCGTTCGCGGTGGGCAGCCAGACGCTCGGGAGCGTGCTGCGCCCCGCCGCTTTCTGC
GGTGTTTGCGGCTTCAAACCGAGTTTCGGGTTGGTCTCTTTCGAAGGGGCGATGCCGTTCGCGCCTTCGCTTGACACGGT
CGGGTTCTTCACGCAGACCGCCGCCGATATGAAGGAGCTCTGTTCGCGGGCTTTTGGCGGCCGGTTTGCCGCCGACCTGC
ATCGCGCGGCAGCGATGCGCGTCAGCGGACGGGAGTGCACGGAGCGGGCGATTGAAGGGTTGCGCGCGGCCGGCGTGACG
ATCGACGAGATCGACCCGCCGGAAGGCTGGGAGCAGCTGCATGCCGCCGCGCGCGTGATCAACCAGTACGAGGGCGCGCG
TACGCAGCGCGCTCGTTACGACGAGTTCGGCGAACAGCTTGGCGCAAAGCTCGCGGCCCTCATCCGCGACGGACTCGCGA
TCCGGGAGGAGGAATACGACGCGGCACAGGCACACGTGGAGCAGATGCGCGGCGAGATCTCGGCGCTGTACTGGGAGTAT
CCAGTGCTGCTGACGCCGGCCGCGACGGGACCGGCCCCCGAGGGTTTCGCCGCTACGGGCGACCCTTCACCGAATGCGCC
GTGGACCGCCATGGGAGTGCCTGCGATCACGGTGCCGCTCCCGGTGGCCGGTGCGCCGCTCGGCCTGCAGATGACGGCCG
CGTGGGGCCGCGACGACGCGCTGGTAGCGGTGGCCGCGCAGTTGGAGTCACTGCTCGGGGGCATCGGGTAA

Upstream 100 bases:

>100_bases
ACCGTGGCGCCCGACGGCCGCTTCCCAGGGAAGATTGTGGAGGCCGGGTTCTTCAACGAGGAGTGGGCGCTCGCTTCGGC
GCCACCCAAGGATCGCTAAC

Downstream 100 bases:

>100_bases
TTCATGGCGCTGCGCGTCGGACTGCTGATCATGCTCGGGGCTGCGTCCGCGAACGGCGCGAACGGATTCGCGGGCGCGGC
GGCCTGCCGGCCATGCCATG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLLQFLRWLESGEKTPQDLLALCLSRVAELDGDVRAWVVVGAQPALAEGALAGIPFGVKDIFETSGLPTEFGSPLYRGRR
GECDAQIVAHLRLAGAVLLGKTETTPFASFDAAPTRNPRLPGHTPGGSSAGSAAAVAAGMVPFAVGSQTLGSVLRPAAFC
GVCGFKPSFGLVSFEGAMPFAPSLDTVGFFTQTAADMKELCSRAFGGRFAADLHRAAAMRVSGRECTERAIEGLRAAGVT
IDEIDPPEGWEQLHAAARVINQYEGARTQRARYDEFGEQLGAKLAALIRDGLAIREEEYDAAQAHVEQMRGEISALYWEY
PVLLTPAATGPAPEGFAATGDPSPNAPWTAMGVPAITVPLPVAGAPLGLQMTAAWGRDDALVAVAAQLESLLGGIG

Sequences:

>Translated_396_residues
MLLQFLRWLESGEKTPQDLLALCLSRVAELDGDVRAWVVVGAQPALAEGALAGIPFGVKDIFETSGLPTEFGSPLYRGRR
GECDAQIVAHLRLAGAVLLGKTETTPFASFDAAPTRNPRLPGHTPGGSSAGSAAAVAAGMVPFAVGSQTLGSVLRPAAFC
GVCGFKPSFGLVSFEGAMPFAPSLDTVGFFTQTAADMKELCSRAFGGRFAADLHRAAAMRVSGRECTERAIEGLRAAGVT
IDEIDPPEGWEQLHAAARVINQYEGARTQRARYDEFGEQLGAKLAALIRDGLAIREEEYDAAQAHVEQMRGEISALYWEY
PVLLTPAATGPAPEGFAATGDPSPNAPWTAMGVPAITVPLPVAGAPLGLQMTAAWGRDDALVAVAAQLESLLGGIG
>Mature_396_residues
MLLQFLRWLESGEKTPQDLLALCLSRVAELDGDVRAWVVVGAQPALAEGALAGIPFGVKDIFETSGLPTEFGSPLYRGRR
GECDAQIVAHLRLAGAVLLGKTETTPFASFDAAPTRNPRLPGHTPGGSSAGSAAAVAAGMVPFAVGSQTLGSVLRPAAFC
GVCGFKPSFGLVSFEGAMPFAPSLDTVGFFTQTAADMKELCSRAFGGRFAADLHRAAAMRVSGRECTERAIEGLRAAGVT
IDEIDPPEGWEQLHAAARVINQYEGARTQRARYDEFGEQLGAKLAALIRDGLAIREEEYDAAQAHVEQMRGEISALYWEY
PVLLTPAATGPAPEGFAATGDPSPNAPWTAMGVPAITVPLPVAGAPLGLQMTAAWGRDDALVAVAAQLESLLGGIG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=228, Percent_Identity=32.0175438596491, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI195972892, Length=404, Percent_Identity=25, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=426, Percent_Identity=25.8215962441315, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=170, Percent_Identity=31.7647058823529, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=162, Percent_Identity=38.8888888888889, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=188, Percent_Identity=32.9787234042553, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648113, Length=423, Percent_Identity=28.1323877068558, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24644968, Length=480, Percent_Identity=24.5833333333333, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI161078093, Length=418, Percent_Identity=24.4019138755981, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 41401; Mature: 41401

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLQFLRWLESGEKTPQDLLALCLSRVAELDGDVRAWVVVGAQPALAEGALAGIPFGVKD
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCHHCCCCCHHH
IFETSGLPTEFGSPLYRGRRGECDAQIVAHLRLAGAVLLGKTETTPFASFDAAPTRNPRL
HHHCCCCCHHHCCHHHCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCC
PGHTPGGSSAGSAAAVAAGMVPFAVGSQTLGSVLRPAAFCGVCGFKPSFGLVSFEGAMPF
CCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
APSLDTVGFFTQTAADMKELCSRAFGGRFAADLHRAAAMRVSGRECTERAIEGLRAAGVT
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCE
IDEIDPPEGWEQLHAAARVINQYEGARTQRARYDEFGEQLGAKLAALIRDGLAIREEEYD
ECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AAQAHVEQMRGEISALYWEYPVLLTPAATGPAPEGFAATGDPSPNAPWTAMGVPAITVPL
HHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEECC
PVAGAPLGLQMTAAWGRDDALVAVAAQLESLLGGIG
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLLQFLRWLESGEKTPQDLLALCLSRVAELDGDVRAWVVVGAQPALAEGALAGIPFGVKD
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCHHCCCCCHHH
IFETSGLPTEFGSPLYRGRRGECDAQIVAHLRLAGAVLLGKTETTPFASFDAAPTRNPRL
HHHCCCCCHHHCCHHHCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCC
PGHTPGGSSAGSAAAVAAGMVPFAVGSQTLGSVLRPAAFCGVCGFKPSFGLVSFEGAMPF
CCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCC
APSLDTVGFFTQTAADMKELCSRAFGGRFAADLHRAAAMRVSGRECTERAIEGLRAAGVT
CCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCE
IDEIDPPEGWEQLHAAARVINQYEGARTQRARYDEFGEQLGAKLAALIRDGLAIREEEYD
ECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AAQAHVEQMRGEISALYWEYPVLLTPAATGPAPEGFAATGDPSPNAPWTAMGVPAITVPL
HHHHHHHHHHHHHHHHHEECCEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCEEEECC
PVAGAPLGLQMTAAWGRDDALVAVAAQLESLLGGIG
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA