Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 116624345

GI number: 116624345

Start: 6598743

End: 6600059

Strand: Direct

Name: gatA [H]

Synonym: Acid_5263

Alternate gene names: 116624345

Gene position: 6598743-6600059 (Clockwise)

Preceding gene: 116624344

Following gene: 116624346

Centisome position: 66.21

GC content: 68.26

Gene sequence:

>1317_bases
GTGCCGTACCGGTCTTCTTTATGCCTGATGGCTTCGATGGTGCGCGACGGGGCGATCTCTCCGCTCGAGTTGGTGGACGC
TCATCTGGCGCAGATAGCCGCGCGCAATTCCGAGATCAATGCTTTCGTGATGGTACTCGCAGAAGAGGCTCGGGAGCGGG
CTCGCTCGCTCGAGCGCGGCGGCCGGCGCGGCCTGCTCTATGGCCTGCCGGTCACCGTCAAGGACAGTTTCGATATGGCG
CGCCTGCCGACCCGCGTTGGGTCGCGCGAGCGTCCGCTGACCCCGGCGGCTCGCGATGCTACGGTGGTCGCCCGCCTGCT
CGCGGAAGGCGCGATCGTGCTCGGGCGCACCAACACGCCCGAAATGCTGGCGCGCTACGACACGGATAACCCGATCACGG
GACGGACCAGCAATCCCTGGGACCTCGACCGCACGCCCGGCGGATCGAGCGGCGGAGAGGCGGCGGCGATCGCGGCATAC
TGTTCGCCAGGCGGAGTGGCGAGCGATGGCGGAGGCTCCATCCGCATGCCGGCGCACTTCTGCGGCATCGCCGGACTCAA
GCCCACACAGGGGCGGATTTCGGGCGCCGGGCACATTCCAGCGCTGGGACATCCGGCGGGCCTGGTGGCCACCGTGGGAC
CGATGGCGCGCACCGCGCAGGATCTCCGGCTGCTGTTTTCCGTGCTCTCCGGCTACGATGCGCAGGATCCTTTTTCGGTG
CCCGTACCGCTGCGGGAGCCGCCGGCCGAGCGCTTGCGAATCGGCGTCTGGGAGCAGTTCTACACGGTGCCGGTGGAACC
GGAGGTGCGAGATGCGGTGCGGCGGGCGGCGGCGCTGGCGAGCGATCTGGGTTTCACCGTGGATGGCTTTGAGCCGCGCG
GCCTGGAGCGCGCGCCCAATCTCTGGAGCTTCCTTTTCGGCCAGTGGGTGGCTAAGGGCAGCGAGCTCACCGCCGCGGAA
GCTCTCAACAATCTGGCCGCTCGCGATCGCATGCGAGCCGCGCTGCTGCGCCAGATGGAAGACGTCGCGGCGGTTCTCAT
GCCGGTCTTCGGGATCACGGCCTTTCGCCATGGGGAGAGCAAATGGCTGGTGGAAGGCAAGGAGATCGGCCTCTTTCAAG
CCGGCATGCCGGCCGTTGTGGCCAACGTGCTCGGGCTGCCCGCGGTCACGATTCCCATCGCAGTCACTGCCGCCGGACTC
CCGGTTGGAGTGCAGTTGATGGGCCGGCCGTTCGAGGACGAACTGCTGCTCGAAGTCGCCATCCAGCTGGAGGAAGCGCG
CGGCGCGTGGCCGGGTCCCGGCGCCTTCGCACGTTAA

Upstream 100 bases:

>100_bases
AGCGGCAGATGAGCATCGAGGGCGATGGCGCGCTTGCGGCGCAACTCCTGTCGCTAACCGCGATCGTTGCCTAGCACCAG
TTCTGTGGCACAATTTCTTC

Downstream 100 bases:

>100_bases
GATAGGAGTAATGTTTCCGAAATTATTTATCCTGTTCGGATTGGCCGCCCTGTGCTTCGGCGCACAGCCGGCCAAGGTCT
TTCCCTATGCTTATACCCAG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MPYRSSLCLMASMVRDGAISPLELVDAHLAQIAARNSEINAFVMVLAEEARERARSLERGGRRGLLYGLPVTVKDSFDMA
RLPTRVGSRERPLTPAARDATVVARLLAEGAIVLGRTNTPEMLARYDTDNPITGRTSNPWDLDRTPGGSSGGEAAAIAAY
CSPGGVASDGGGSIRMPAHFCGIAGLKPTQGRISGAGHIPALGHPAGLVATVGPMARTAQDLRLLFSVLSGYDAQDPFSV
PVPLREPPAERLRIGVWEQFYTVPVEPEVRDAVRRAAALASDLGFTVDGFEPRGLERAPNLWSFLFGQWVAKGSELTAAE
ALNNLAARDRMRAALLRQMEDVAAVLMPVFGITAFRHGESKWLVEGKEIGLFQAGMPAVVANVLGLPAVTIPIAVTAAGL
PVGVQLMGRPFEDELLLEVAIQLEEARGAWPGPGAFAR

Sequences:

>Translated_438_residues
MPYRSSLCLMASMVRDGAISPLELVDAHLAQIAARNSEINAFVMVLAEEARERARSLERGGRRGLLYGLPVTVKDSFDMA
RLPTRVGSRERPLTPAARDATVVARLLAEGAIVLGRTNTPEMLARYDTDNPITGRTSNPWDLDRTPGGSSGGEAAAIAAY
CSPGGVASDGGGSIRMPAHFCGIAGLKPTQGRISGAGHIPALGHPAGLVATVGPMARTAQDLRLLFSVLSGYDAQDPFSV
PVPLREPPAERLRIGVWEQFYTVPVEPEVRDAVRRAAALASDLGFTVDGFEPRGLERAPNLWSFLFGQWVAKGSELTAAE
ALNNLAARDRMRAALLRQMEDVAAVLMPVFGITAFRHGESKWLVEGKEIGLFQAGMPAVVANVLGLPAVTIPIAVTAAGL
PVGVQLMGRPFEDELLLEVAIQLEEARGAWPGPGAFAR
>Mature_437_residues
PYRSSLCLMASMVRDGAISPLELVDAHLAQIAARNSEINAFVMVLAEEARERARSLERGGRRGLLYGLPVTVKDSFDMAR
LPTRVGSRERPLTPAARDATVVARLLAEGAIVLGRTNTPEMLARYDTDNPITGRTSNPWDLDRTPGGSSGGEAAAIAAYC
SPGGVASDGGGSIRMPAHFCGIAGLKPTQGRISGAGHIPALGHPAGLVATVGPMARTAQDLRLLFSVLSGYDAQDPFSVP
VPLREPPAERLRIGVWEQFYTVPVEPEVRDAVRRAAALASDLGFTVDGFEPRGLERAPNLWSFLFGQWVAKGSELTAAEA
LNNLAARDRMRAALLRQMEDVAAVLMPVFGITAFRHGESKWLVEGKEIGLFQAGMPAVVANVLGLPAVTIPIAVTAAGLP
VGVQLMGRPFEDELLLEVAIQLEEARGAWPGPGAFAR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=486, Percent_Identity=27.5720164609054, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI166795287, Length=279, Percent_Identity=35.84229390681, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI222831590, Length=313, Percent_Identity=27.1565495207668, Blast_Score=102, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=485, Percent_Identity=30.3092783505155, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17556264, Length=281, Percent_Identity=32.0284697508897, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538252, Length=325, Percent_Identity=29.5384615384615, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71990152, Length=294, Percent_Identity=31.2925170068027, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17556276, Length=250, Percent_Identity=36.8, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17556278, Length=250, Percent_Identity=36.8, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17543272, Length=445, Percent_Identity=26.5168539325843, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538254, Length=294, Percent_Identity=30.2721088435374, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32565337, Length=377, Percent_Identity=26.790450928382, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=249, Percent_Identity=28.5140562248996, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=212, Percent_Identity=31.6037735849057, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=455, Percent_Identity=25.0549450549451, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=29.5180722891566, Blast_Score=162, Evalue=5e-40,
Organism=Drosophila melanogaster, GI45550774, Length=233, Percent_Identity=40.343347639485, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24648435, Length=233, Percent_Identity=40.343347639485, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI24648437, Length=233, Percent_Identity=40.343347639485, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI161078093, Length=491, Percent_Identity=29.3279022403259, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24648441, Length=166, Percent_Identity=45.7831325301205, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648439, Length=166, Percent_Identity=45.7831325301205, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21356731, Length=231, Percent_Identity=37.2294372294372, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652985, Length=486, Percent_Identity=27.9835390946502, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922090, Length=486, Percent_Identity=27.9835390946502, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652981, Length=486, Percent_Identity=27.9835390946502, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24652983, Length=486, Percent_Identity=27.9835390946502, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648113, Length=292, Percent_Identity=31.5068493150685, Blast_Score=95, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 46551; Mature: 46420

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYRSSLCLMASMVRDGAISPLELVDAHLAQIAARNSEINAFVMVLAEEARERARSLERG
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GRRGLLYGLPVTVKDSFDMARLPTRVGSRERPLTPAARDATVVARLLAEGAIVLGRTNTP
CCCCEEEECCEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCH
EMLARYDTDNPITGRTSNPWDLDRTPGGSSGGEAAAIAAYCSPGGVASDGGGSIRMPAHF
HHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEECCHHH
CGIAGLKPTQGRISGAGHIPALGHPAGLVATVGPMARTAQDLRLLFSVLSGYDAQDPFSV
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
PVPLREPPAERLRIGVWEQFYTVPVEPEVRDAVRRAAALASDLGFTVDGFEPRGLERAPN
CCCCCCCCHHHHEEHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCH
LWSFLFGQWVAKGSELTAAEALNNLAARDRMRAALLRQMEDVAAVLMPVFGITAFRHGES
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KWLVEGKEIGLFQAGMPAVVANVLGLPAVTIPIAVTAAGLPVGVQLMGRPFEDELLLEVA
CEEEECCCCCEECCCHHHHHHHHHCCCHHHHHHHHEECCCCCCHHCCCCCCHHHHHHHHH
IQLEEARGAWPGPGAFAR
HHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PYRSSLCLMASMVRDGAISPLELVDAHLAQIAARNSEINAFVMVLAEEARERARSLERG
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
GRRGLLYGLPVTVKDSFDMARLPTRVGSRERPLTPAARDATVVARLLAEGAIVLGRTNTP
CCCCEEEECCEEEECCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCH
EMLARYDTDNPITGRTSNPWDLDRTPGGSSGGEAAAIAAYCSPGGVASDGGGSIRMPAHF
HHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEECCHHH
CGIAGLKPTQGRISGAGHIPALGHPAGLVATVGPMARTAQDLRLLFSVLSGYDAQDPFSV
HCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
PVPLREPPAERLRIGVWEQFYTVPVEPEVRDAVRRAAALASDLGFTVDGFEPRGLERAPN
CCCCCCCCHHHHEEHHHHHHEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCH
LWSFLFGQWVAKGSELTAAEALNNLAARDRMRAALLRQMEDVAAVLMPVFGITAFRHGES
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KWLVEGKEIGLFQAGMPAVVANVLGLPAVTIPIAVTAAGLPVGVQLMGRPFEDELLLEVA
CEEEECCCCCEECCCHHHHHHHHHCCCHHHHHHHHEECCCCCCHHCCCCCCHHHHHHHHH
IQLEEARGAWPGPGAFAR
HHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA