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The map label for this gene is punA [H]

Identifier: 116624315

GI number: 116624315

Start: 6567945

End: 6568754

Strand: Direct

Name: punA [H]

Synonym: Acid_5233

Alternate gene names: 116624315

Gene position: 6567945-6568754 (Clockwise)

Preceding gene: 116624314

Following gene: 116624316

Centisome position: 65.91

GC content: 64.57

Gene sequence:

>810_bases
GTGACGGACGAGGCAAAGCGGTTTATTCAAGGCCACACGAGTGTGCGGCCGTCCATCGGCGTCGTGTTGGGGAGCGGGCT
TGGGGCGTTTGCCGACGAGCTCACGGAGCGCATCGATATCCCGTACGCGGATATTCCGGGCTGGCCGCATTCGACCGCGG
TGGGCCACGCGGGCAAGCTGATTATCGGTAAGCTGGGCGGGCTGGACGTGGCGGTGATGGCGGGGCGCGCACATCTGTAC
GAAGGCTACGCGATGCACCAGGTGACGTACGGGGTGCGGGTGCTGCAGGCAATCGGGACACGCTCCATGGTGTTCACGAA
CGCGGCGGGCGGCATCAACCTGACGTTGGAACGCGGCGGGCTGGTACTCATCAGTGACCACATCAATCTGCAGGGCGGGA
ATCCGCTGGCTGGACCGAACGACGACCGGCTGGGGCCGCGCTTCCCGGACATGTCGGAGGCATACTCGCGCGCTTATCGC
GCAGTCGCAAAAGAGGTCGCGGCGGAACTTTGCGTTGCCATCACGGAAGGCGTGTATGCGGCGATGCTGGGACCGAGCTA
CGAAACTCCGGCGGAGGTCCGCTACCTGCGGACGATCGGCGCGGACGTGGTGGGGATGTCCACGGTGCCGGAAGTGATCG
TGGCGAACCACATGGGGATGAAGGTGCTCGGGATCTCCTGCGTGACGAATATGGCGGCGGGGATTCTGCCGCAGAAGATC
AATCACGAAGAGGTGCTGGAGACCGGCGCGATGGTGCGCGACACGCTGGTGCGTTTCCTGAAGGCCCTGCTGCCAAGGTT
GGACGCATGA

Upstream 100 bases:

>100_bases
CGCCGACGCGCAAGAGCGACCTGGCGAGGCTGGGACTGCGCGCGGTGGCGGCGGGGACGATGGCGAACTTCATGTCGGCG
TGCATTGCGGGAATGTTGCT

Downstream 100 bases:

>100_bases
CCTCCTCGGGCGACCAGTTGGTGAACGCGGCGCTGGCAGTACGGCTGAATGCCTTCGCGCCGTACTCGAAATTCCTGGTG
GGCGCCGCGATTGAAGATGT

Product: inosine guanosine and xanthosine phosphorylase family protein

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTDEAKRFIQGHTSVRPSIGVVLGSGLGAFADELTERIDIPYADIPGWPHSTAVGHAGKLIIGKLGGLDVAVMAGRAHLY
EGYAMHQVTYGVRVLQAIGTRSMVFTNAAGGINLTLERGGLVLISDHINLQGGNPLAGPNDDRLGPRFPDMSEAYSRAYR
AVAKEVAAELCVAITEGVYAAMLGPSYETPAEVRYLRTIGADVVGMSTVPEVIVANHMGMKVLGISCVTNMAAGILPQKI
NHEEVLETGAMVRDTLVRFLKALLPRLDA

Sequences:

>Translated_269_residues
MTDEAKRFIQGHTSVRPSIGVVLGSGLGAFADELTERIDIPYADIPGWPHSTAVGHAGKLIIGKLGGLDVAVMAGRAHLY
EGYAMHQVTYGVRVLQAIGTRSMVFTNAAGGINLTLERGGLVLISDHINLQGGNPLAGPNDDRLGPRFPDMSEAYSRAYR
AVAKEVAAELCVAITEGVYAAMLGPSYETPAEVRYLRTIGADVVGMSTVPEVIVANHMGMKVLGISCVTNMAAGILPQKI
NHEEVLETGAMVRDTLVRFLKALLPRLDA
>Mature_268_residues
TDEAKRFIQGHTSVRPSIGVVLGSGLGAFADELTERIDIPYADIPGWPHSTAVGHAGKLIIGKLGGLDVAVMAGRAHLYE
GYAMHQVTYGVRVLQAIGTRSMVFTNAAGGINLTLERGGLVLISDHINLQGGNPLAGPNDDRLGPRFPDMSEAYSRAYRA
VAKEVAAELCVAITEGVYAAMLGPSYETPAEVRYLRTIGADVVGMSTVPEVIVANHMGMKVLGISCVTNMAAGILPQKIN
HEEVLETGAMVRDTLVRFLKALLPRLDA

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=265, Percent_Identity=46.7924528301887, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI47132622, Length=228, Percent_Identity=28.5087719298246, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788746, Length=251, Percent_Identity=41.8326693227092, Blast_Score=216, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI17541190, Length=263, Percent_Identity=40.6844106463878, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323238, Length=274, Percent_Identity=39.051094890511, Blast_Score=183, Evalue=2e-47,
Organism=Drosophila melanogaster, GI45552885, Length=271, Percent_Identity=40.590405904059, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24656090, Length=271, Percent_Identity=40.590405904059, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI45552887, Length=271, Percent_Identity=40.590405904059, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24656093, Length=271, Percent_Identity=40.590405904059, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24762376, Length=232, Percent_Identity=32.7586206896552, Blast_Score=125, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 28575; Mature: 28444

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEAKRFIQGHTSVRPSIGVVLGSGLGAFADELTERIDIPYADIPGWPHSTAVGHAGKL
CCHHHHHHHHCCCCCCCCHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
IIGKLGGLDVAVMAGRAHLYEGYAMHQVTYGVRVLQAIGTRSMVFTNAAGGINLTLERGG
EEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCC
LVLISDHINLQGGNPLAGPNDDRLGPRFPDMSEAYSRAYRAVAKEVAAELCVAITEGVYA
EEEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMLGPSYETPAEVRYLRTIGADVVGMSTVPEVIVANHMGMKVLGISCVTNMAAGILPQKI
HHCCCCCCCHHHHHHHHHHCCCHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
NHEEVLETGAMVRDTLVRFLKALLPRLDA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDEAKRFIQGHTSVRPSIGVVLGSGLGAFADELTERIDIPYADIPGWPHSTAVGHAGKL
CHHHHHHHHCCCCCCCCHHEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCE
IIGKLGGLDVAVMAGRAHLYEGYAMHQVTYGVRVLQAIGTRSMVFTNAAGGINLTLERGG
EEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCC
LVLISDHINLQGGNPLAGPNDDRLGPRFPDMSEAYSRAYRAVAKEVAAELCVAITEGVYA
EEEEECCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMLGPSYETPAEVRYLRTIGADVVGMSTVPEVIVANHMGMKVLGISCVTNMAAGILPQKI
HHCCCCCCCHHHHHHHHHHCCCHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
NHEEVLETGAMVRDTLVRFLKALLPRLDA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]