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The map label for this gene is sthA [H]

Identifier: 116624142

GI number: 116624142

Start: 6390498

End: 6391904

Strand: Direct

Name: sthA [H]

Synonym: Acid_5058

Alternate gene names: 116624142

Gene position: 6390498-6391904 (Clockwise)

Preceding gene: 116624141

Following gene: 116624143

Centisome position: 64.13

GC content: 62.12

Gene sequence:

>1407_bases
ATGACTGACACGTATGACCTGATCGCGATCGGATCGGGTCCGGCCGGCGAAAGCGCTACCGAGTTGGCAGCGTTCTTCGG
GCACCGCTGTGCGGTAGTCGAGAGAGCCCGTCCTGGCGGAACCGTAACCACGACCGGCGGAGCGCCGACAAAAACGCTCC
GTGAGGCGGCTCTCTATTTCTCGGGACTGGTCGATGGCGATGTTTACGGGATAAAGATCTCTACTCCTCCGGAAGTGGCG
ACGGAAGTGATCCGTAAACGCACATGGCACGTCTGCGATCTGCTCCAAAAGGTGACGGGCGAGAACATCGCCAAAAACAA
TGTCGACTACATCCAGGGCGCGGCGCGGCTCGAGGGCGACGGGAAGGTGATGGTCACGGGCGATGACGGATCCCGGCGCA
GGCTCCGCGCGAACGTTTTGCTGCTTGCGACAGGATCGAGGCCGCGGCGGCCGCCGAACATACCATTCGATCTCGCCGGA
GTTTGCGACACGGACACGATCCTGCAACGCGGCCGGGTTCCCAAGGACATCCTGATTGTCGGCGGCGGACCAGTCGGCGT
CGAGTTTGCGACCATAGCGCATGCGCTGGGCGCGAAAGTCACGCTGGCAGATCGCGGAAATCGCCTGATGTCGATGATGG
ATGGCGAGATTACAGAGTGCATGGAAGAACTGTTCCGGACCTGGGGCATCCGAGTCCTGTTCGGATCGACCTGCAATAGC
GTGGTCGCTAAGGACGATGCTCTGGAGATCACGATGTCTACCGGCGAAAGGCTCTCTCCAGACACGGTCTTGTTCGCGGC
GGGACGCGTCCCAAACACCGGGGACTTGGGTCTCGATGCGGCGGGAGTGGCCATGGACGCCCGTGGTCGCATCGTCGTCG
ATCAGCAATTTCGCACCTCTGCGGAAGGTGTGTACGCTGCCGGCGACGTTCTTGGACCGACCCTGGCCTCGATCGCCATG
GAGCAGGGCCGCGTGGCGATTTGCCATGCCTTCGGCATTCCTTTCGAGGGAATCGTGGATCCGGCGCCGGTATCCGCGGT
CTACGGCATGCCCGAGGTTTCGGGCGCGGGTCGCACCGAAGAGGAGTGCCGCGAAATGGGCGTTGACTATGAGGTTGGAC
GGGCGGACCTTGCGCGCACGCCGCGCGGTGCGATCGCCGGACGGGGCGGGAGGCTGAAACTGATCTTCCAAAAGGAAGAT
CGCAGGCTGATCGGCGTGCACTGCATCGGCGATATCGCCTCGGAAATCGTCGGGATCGGCCAGATGACCATCCGGTGCGG
CGGAACCATCCATACCATCGCCAACATGTCGTTCAACACGCCGACATACAGTTATGCGTACAAGTACGCCGCATTTGACG
GCTTGCGCAAGCTGGCGGCGCAGGGGCAGACGGGAGAATACAAGTGA

Upstream 100 bases:

>100_bases
AGTACGGCGAGTATCCGTACGCCAGCACGGACGAAATCAAAAAGGCTCTCCGGCTCAAGCAGGCCTTCTCGGAAGCGCTC
GACCAGATGCAGTGAGCGCA

Downstream 100 bases:

>100_bases
ATCAATCGATAGACGCCGCGCGGGTCCACAACCACTACAGCGTCCCGCGGAAATACATCTCCGGCAAGCGGCGCTTTAGC
GTCTACTGGACCTGGAGCTA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: STH; NAD(P)(+) transhydrogenase [B-specific] [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTDTYDLIAIGSGPAGESATELAAFFGHRCAVVERARPGGTVTTTGGAPTKTLREAALYFSGLVDGDVYGIKISTPPEVA
TEVIRKRTWHVCDLLQKVTGENIAKNNVDYIQGAARLEGDGKVMVTGDDGSRRRLRANVLLLATGSRPRRPPNIPFDLAG
VCDTDTILQRGRVPKDILIVGGGPVGVEFATIAHALGAKVTLADRGNRLMSMMDGEITECMEELFRTWGIRVLFGSTCNS
VVAKDDALEITMSTGERLSPDTVLFAAGRVPNTGDLGLDAAGVAMDARGRIVVDQQFRTSAEGVYAAGDVLGPTLASIAM
EQGRVAICHAFGIPFEGIVDPAPVSAVYGMPEVSGAGRTEEECREMGVDYEVGRADLARTPRGAIAGRGGRLKLIFQKED
RRLIGVHCIGDIASEIVGIGQMTIRCGGTIHTIANMSFNTPTYSYAYKYAAFDGLRKLAAQGQTGEYK

Sequences:

>Translated_468_residues
MTDTYDLIAIGSGPAGESATELAAFFGHRCAVVERARPGGTVTTTGGAPTKTLREAALYFSGLVDGDVYGIKISTPPEVA
TEVIRKRTWHVCDLLQKVTGENIAKNNVDYIQGAARLEGDGKVMVTGDDGSRRRLRANVLLLATGSRPRRPPNIPFDLAG
VCDTDTILQRGRVPKDILIVGGGPVGVEFATIAHALGAKVTLADRGNRLMSMMDGEITECMEELFRTWGIRVLFGSTCNS
VVAKDDALEITMSTGERLSPDTVLFAAGRVPNTGDLGLDAAGVAMDARGRIVVDQQFRTSAEGVYAAGDVLGPTLASIAM
EQGRVAICHAFGIPFEGIVDPAPVSAVYGMPEVSGAGRTEEECREMGVDYEVGRADLARTPRGAIAGRGGRLKLIFQKED
RRLIGVHCIGDIASEIVGIGQMTIRCGGTIHTIANMSFNTPTYSYAYKYAAFDGLRKLAAQGQTGEYK
>Mature_467_residues
TDTYDLIAIGSGPAGESATELAAFFGHRCAVVERARPGGTVTTTGGAPTKTLREAALYFSGLVDGDVYGIKISTPPEVAT
EVIRKRTWHVCDLLQKVTGENIAKNNVDYIQGAARLEGDGKVMVTGDDGSRRRLRANVLLLATGSRPRRPPNIPFDLAGV
CDTDTILQRGRVPKDILIVGGGPVGVEFATIAHALGAKVTLADRGNRLMSMMDGEITECMEELFRTWGIRVLFGSTCNSV
VAKDDALEITMSTGERLSPDTVLFAAGRVPNTGDLGLDAAGVAMDARGRIVVDQQFRTSAEGVYAAGDVLGPTLASIAME
QGRVAICHAFGIPFEGIVDPAPVSAVYGMPEVSGAGRTEEECREMGVDYEVGRADLARTPRGAIAGRGGRLKLIFQKEDR
RLIGVHCIGDIASEIVGIGQMTIRCGGTIHTIANMSFNTPTYSYAYKYAAFDGLRKLAAQGQTGEYK

Specific function: Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=28.3842794759825, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=29.4117647058824, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI22035672, Length=468, Percent_Identity=28.6324786324786, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI291045266, Length=454, Percent_Identity=25.9911894273128, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI291045268, Length=447, Percent_Identity=25.2796420581655, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI33519430, Length=469, Percent_Identity=24.7334754797441, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI33519428, Length=469, Percent_Identity=24.7334754797441, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI33519426, Length=469, Percent_Identity=24.7334754797441, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI148277065, Length=465, Percent_Identity=25.1612903225806, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI148277071, Length=469, Percent_Identity=24.7334754797441, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=33.1168831168831, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1786307, Length=462, Percent_Identity=30.952380952381, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1789915, Length=452, Percent_Identity=28.7610619469027, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=26.9911504424779, Blast_Score=135, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=465, Percent_Identity=27.9569892473118, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17557007, Length=471, Percent_Identity=27.6008492569002, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=352, Percent_Identity=28.4090909090909, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71983419, Length=352, Percent_Identity=28.4090909090909, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71982272, Length=453, Percent_Identity=25.60706401766, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321091, Length=483, Percent_Identity=28.9855072463768, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6325240, Length=469, Percent_Identity=25.7995735607676, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325166, Length=450, Percent_Identity=26.4444444444444, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI21358499, Length=462, Percent_Identity=28.3549783549784, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24640553, Length=463, Percent_Identity=26.7818574514039, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24640549, Length=463, Percent_Identity=26.7818574514039, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI24640551, Length=463, Percent_Identity=26.7818574514039, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17737741, Length=469, Percent_Identity=27.7185501066098, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327
- InterPro:   IPR022962 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.1.1 [H]

Molecular weight: Translated: 49722; Mature: 49591

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTYDLIAIGSGPAGESATELAAFFGHRCAVVERARPGGTVTTTGGAPTKTLREAALYF
CCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHH
SGLVDGDVYGIKISTPPEVATEVIRKRTWHVCDLLQKVTGENIAKNNVDYIQGAARLEGD
HCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEECCC
GKVMVTGDDGSRRRLRANVLLLATGSRPRRPPNIPFDLAGVCDTDTILQRGRVPKDILIV
CEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEE
GGGPVGVEFATIAHALGAKVTLADRGNRLMSMMDGEITECMEELFRTWGIRVLFGSTCNS
CCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCH
VVAKDDALEITMSTGERLSPDTVLFAAGRVPNTGDLGLDAAGVAMDARGRIVVDQQFRTS
HHCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCC
AEGVYAAGDVLGPTLASIAMEQGRVAICHAFGIPFEGIVDPAPVSAVYGMPEVSGAGRTE
CCCEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCH
EECREMGVDYEVGRADLARTPRGAIAGRGGRLKLIFQKEDRRLIGVHCIGDIASEIVGIG
HHHHHCCCCCCCCCHHHCCCCCCCEECCCCEEEEEEEECCCEEEEEEEHHHHHHHHHCCC
QMTIRCGGTIHTIANMSFNTPTYSYAYKYAAFDGLRKLAAQGQTGEYK
EEEEEECCEEEEEEECCCCCCCHHHEEHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDTYDLIAIGSGPAGESATELAAFFGHRCAVVERARPGGTVTTTGGAPTKTLREAALYF
CCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCEEECCCCCHHHHHHHHHHH
SGLVDGDVYGIKISTPPEVATEVIRKRTWHVCDLLQKVTGENIAKNNVDYIQGAARLEGD
HCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCEEECCC
GKVMVTGDDGSRRRLRANVLLLATGSRPRRPPNIPFDLAGVCDTDTILQRGRVPKDILIV
CEEEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEE
GGGPVGVEFATIAHALGAKVTLADRGNRLMSMMDGEITECMEELFRTWGIRVLFGSTCNS
CCCCCCHHHHHHHHHHCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCCH
VVAKDDALEITMSTGERLSPDTVLFAAGRVPNTGDLGLDAAGVAMDARGRIVVDQQFRTS
HHCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCC
AEGVYAAGDVLGPTLASIAMEQGRVAICHAFGIPFEGIVDPAPVSAVYGMPEVSGAGRTE
CCCEEEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCCH
EECREMGVDYEVGRADLARTPRGAIAGRGGRLKLIFQKEDRRLIGVHCIGDIASEIVGIG
HHHHHCCCCCCCCCHHHCCCCCCCEECCCCEEEEEEEECCCEEEEEEEHHHHHHHHHCCC
QMTIRCGGTIHTIANMSFNTPTYSYAYKYAAFDGLRKLAAQGQTGEYK
EEEEEECCEEEEEEECCCCCCCHHHEEHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]