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The map label for this gene is purK [H]

Identifier: 116624127

GI number: 116624127

Start: 6367939

End: 6369084

Strand: Direct

Name: purK [H]

Synonym: Acid_5043

Alternate gene names: 116624127

Gene position: 6367939-6369084 (Clockwise)

Preceding gene: 116624126

Following gene: 116624130

Centisome position: 63.9

GC content: 68.85

Gene sequence:

>1146_bases
ATGAAGCCCGTCCTCCCCGGCGCCACCGTCGGCATCCTCGGCGGCGGACAGCTCGGACGCATGTTCGCCATCGCCGCTCG
CCGCATGGGCTATCGCGTACACACGTTCGCCCCCGATGTCGATACCCCCGCCGGCCAGATCTCCGACGTTGAAATCCACG
CGCCCTACGAGGATCTCGATACCGTCCGCGCCTTCGCCCGCAGAGTCGACGTGGTCACCTTCGAATTCGAGAACGTGCCT
GCCGCCACTGCCGCGGCCGCCGCCGAATTCGCGCCCGTCCGTCCCGCCGGCGCCGTGCTCCACACCACCCAGCACCGTCT
CCGCGAGAAGCTCTTCCTGCAGGGCGCCGGACTCCCGCTCGCACCATTCCGCATCGTCCGCACCGCCGGCGATCTCGCTC
GCGCGGTCGCCGATCTCGGCTGCCCCGCCGTCCTCAAAACAGCCGGCTTCGGCTACGACGGTCACGGCCAGGTGCGTATC
ACGTCAGCCGACACCGCCCTCGCCGCCTGGCAATCCATCGGCTCCCAGGAAGCCGTGCTCGAGGCCTTCATCGATTTCGA
GTGCGAGCTCTCCGTGGTCGCGGCGCGCGCCGCCGATGGCGCCTTCGTGCATTACGGCGTGGTGGAAAACCGCCACCAGC
GCCACATCCTCGATATCACCATTGCGCCCGCCACCGTGGCGCCGCAGGTCGCGCCCCGGGCCATCGAAATCGCGCGCATC
GTGATGGAGACGCTGGACGCCGTCGGCGTACTCTGCGTCGAATTCTTCCTGACCCGCGCCGGCGACCTGCTCATCAACGA
GCTCGCCCCGCGTCCGCACAACTCCGGCCACTTCACCTTCGACGCCTGCGTTACCAGCCAGTTCGAACAGCAGCTCCGCG
CCGTCTGTGGCCTGCCGCTCGGCTCCACGGAACAGATGCGGCCCGCCGCCATGGCCAACCTGCTCGGCGATCTCTGGACC
GCCGGCCCACCGGATTGGGCCGCCGCCTGCGCATTCCACGGCGTGAAGCTCCACCTCTATGGCAAGGCCGAGCCTCGCCC
AGGACGCAAAATGGGACACCTCACCGCGCTCGGTCCCGACCAGGCCACTGCGCTAAAGACGGTCCTCGACGCGCGAGACT
CCATGGTCGGCGTTCACCGCACGTAG

Upstream 100 bases:

>100_bases
TCCTCGCCGTCGCCATCCTCGCCAACACTCGCCCCGAACTGCGCGAAAAACTCCGCCGTTATCGCCAGGAGCAGACCGCG
CGCGTGGAGCGGGAGACGCT

Downstream 100 bases:

>100_bases
ACCTGCACCGCATTGCCCACCAAGTCCCTGGAGTGCAGCGTGAACGGCCCGCTGAAAACCGCCGGCAATTTCAGATTCAT
CTGAAAGACTCCGTAAATCA

Product: phosphoribosylaminoimidazole carboxylase

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKPVLPGATVGILGGGQLGRMFAIAARRMGYRVHTFAPDVDTPAGQISDVEIHAPYEDLDTVRAFARRVDVVTFEFENVP
AATAAAAAEFAPVRPAGAVLHTTQHRLREKLFLQGAGLPLAPFRIVRTAGDLARAVADLGCPAVLKTAGFGYDGHGQVRI
TSADTALAAWQSIGSQEAVLEAFIDFECELSVVAARAADGAFVHYGVVENRHQRHILDITIAPATVAPQVAPRAIEIARI
VMETLDAVGVLCVEFFLTRAGDLLINELAPRPHNSGHFTFDACVTSQFEQQLRAVCGLPLGSTEQMRPAAMANLLGDLWT
AGPPDWAAACAFHGVKLHLYGKAEPRPGRKMGHLTALGPDQATALKTVLDARDSMVGVHRT

Sequences:

>Translated_381_residues
MKPVLPGATVGILGGGQLGRMFAIAARRMGYRVHTFAPDVDTPAGQISDVEIHAPYEDLDTVRAFARRVDVVTFEFENVP
AATAAAAAEFAPVRPAGAVLHTTQHRLREKLFLQGAGLPLAPFRIVRTAGDLARAVADLGCPAVLKTAGFGYDGHGQVRI
TSADTALAAWQSIGSQEAVLEAFIDFECELSVVAARAADGAFVHYGVVENRHQRHILDITIAPATVAPQVAPRAIEIARI
VMETLDAVGVLCVEFFLTRAGDLLINELAPRPHNSGHFTFDACVTSQFEQQLRAVCGLPLGSTEQMRPAAMANLLGDLWT
AGPPDWAAACAFHGVKLHLYGKAEPRPGRKMGHLTALGPDQATALKTVLDARDSMVGVHRT
>Mature_381_residues
MKPVLPGATVGILGGGQLGRMFAIAARRMGYRVHTFAPDVDTPAGQISDVEIHAPYEDLDTVRAFARRVDVVTFEFENVP
AATAAAAAEFAPVRPAGAVLHTTQHRLREKLFLQGAGLPLAPFRIVRTAGDLARAVADLGCPAVLKTAGFGYDGHGQVRI
TSADTALAAWQSIGSQEAVLEAFIDFECELSVVAARAADGAFVHYGVVENRHQRHILDITIAPATVAPQVAPRAIEIARI
VMETLDAVGVLCVEFFLTRAGDLLINELAPRPHNSGHFTFDACVTSQFEQQLRAVCGLPLGSTEQMRPAAMANLLGDLWT
AGPPDWAAACAFHGVKLHLYGKAEPRPGRKMGHLTALGPDQATALKTVLDARDSMVGVHRT

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=359, Percent_Identity=33.7047353760446, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1788155, Length=372, Percent_Identity=26.8817204301075, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324702, Length=368, Percent_Identity=34.2391304347826, Blast_Score=193, Evalue=4e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40612; Mature: 40612

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVLPGATVGILGGGQLGRMFAIAARRMGYRVHTFAPDVDTPAGQISDVEIHAPYEDLD
CCCCCCCCCEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCHHHHH
TVRAFARRVDVVTFEFENVPAATAAAAAEFAPVRPAGAVLHTTQHRLREKLFLQGAGLPL
HHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
APFRIVRTAGDLARAVADLGCPAVLKTAGFGYDGHGQVRITSADTALAAWQSIGSQEAVL
CHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECHHHHHHHHHCCCHHHHH
EAFIDFECELSVVAARAADGAFVHYGVVENRHQRHILDITIAPATVAPQVAPRAIEIARI
HHHHCCCCEEEEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHH
VMETLDAVGVLCVEFFLTRAGDLLINELAPRPHNSGHFTFDACVTSQFEQQLRAVCGLPL
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCC
GSTEQMRPAAMANLLGDLWTAGPPDWAAACAFHGVKLHLYGKAEPRPGRKMGHLTALGPD
CCCHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEECCCC
QATALKTVLDARDSMVGVHRT
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKPVLPGATVGILGGGQLGRMFAIAARRMGYRVHTFAPDVDTPAGQISDVEIHAPYEDLD
CCCCCCCCCEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEECCHHHHH
TVRAFARRVDVVTFEFENVPAATAAAAAEFAPVRPAGAVLHTTQHRLREKLFLQGAGLPL
HHHHHHHHHEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
APFRIVRTAGDLARAVADLGCPAVLKTAGFGYDGHGQVRITSADTALAAWQSIGSQEAVL
CHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEEECHHHHHHHHHCCCHHHHH
EAFIDFECELSVVAARAADGAFVHYGVVENRHQRHILDITIAPATVAPQVAPRAIEIARI
HHHHCCCCEEEEEEEECCCCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHH
VMETLDAVGVLCVEFFLTRAGDLLINELAPRPHNSGHFTFDACVTSQFEQQLRAVCGLPL
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCC
GSTEQMRPAAMANLLGDLWTAGPPDWAAACAFHGVKLHLYGKAEPRPGRKMGHLTALGPD
CCCHHCCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCEEECCCC
QATALKTVLDARDSMVGVHRT
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]