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The map label for this gene is gatA [H]

Identifier: 116624038

GI number: 116624038

Start: 6249209

End: 6250975

Strand: Reverse

Name: gatA [H]

Synonym: Acid_4952

Alternate gene names: 116624038

Gene position: 6250975-6249209 (Counterclockwise)

Preceding gene: 116624039

Following gene: 116624035

Centisome position: 62.73

GC content: 64.4

Gene sequence:

>1767_bases
ATGACCCCTGACAAAATGCGTAGACGATTTCTGGCCTGCTGCTCCGGTATCGGACTGGCCGGCACGCTTCTGCCCGGTGT
GCTCTGGGCTCAATTGCAGCAGGATGGCGCGCAGCAGGTCACGCCCGAAATGCTGAAGGCAGCCCTGGGGCTATCCGGCC
TCTCATTCAGCGAGAGCGATCAGAAGTCCATGCTGCAGGCGGTCAATGGCAGCCTGGCGCGGTACGACGAAGTTCGCAAT
CTTCACATCCCCAACAACGTCGCGCCGCCGTTTTATTTCAGCGCCCTGACTCCGGGCATGAAGGTCAATCGCACCCGCGA
ACCGCTCCGCTTCAGCACTCCCGCGGTCAAGCGCCCGGCCAACCTGGAGGATGTCGCGTTCTGGCCCGTCACGCACCTGG
CGCAGCTCATCAAGACCCGCCAGGTCACGTCCACCGAGCTCACGAAAATGTACCTGGCGCGCCTGCACAAGTACAACGAA
AAGGTGAATTGCGTGGTCACCTTTCTCGATGAGGTGGCGCTGGCGCAGGCAAAGCAGGCCGATGCGGAAATCGCCGCCGG
CCGGTACAAGGGAGCGCTGCACGGCATCCCATGGGGAGCCAAGGACATCATCGCGGTCAAAGGTTACAAGACCACCTGGG
GCTCCGGTGCGTACAAAGAACAGATGCTCGATGAAGAGGCCAGCCTGGTCGAGATGCTGCGCGATGCCGGCGCGGTCCTG
CTTGCCAAGCTGACCACAGGGGAACTGGCGCAGGGCGACCAGTGGTTCGGCGGCCAGACCAAAAATCCCTGGAACATCGA
ACAGGGTTCCAGCGGGTCTTCCGCGGGCCCGGCATCCGCCACCGCTGGTGGGCTGGTGGGCTTCGGCATCGGCAGCGAGA
CCAGCGGGTCGATCCTCAGCCCCTCCGCTCGCTGCGGCGTCACCGGCCTGCGCCCCACTTTCGGACGCATCAGCCGTTAT
GGCGTGATGGCGCTCTCCTGGACGCAGGACCGCATGGGTCCGCTGTGCCGCTATGCCGAGGATTGCGCTCTGGTGATGAG
CGTGATCGCGCGGCCCGACGGCCGCGACCTCAGCGTCTCCGAGATTCCCTTCAACTGGAATGCCCACCTGGATGTCCGCA
AGTTGCGTGTCGGTTACCTGGAGGGAGCCTTCGAGGAAACGCGCGACGCCGCCGTCAAGAAGATCGAAGAGCGCACCCTG
GAGCAGGTGAAGGCGCTCGGTATCCAACTGGTGCCGGTGAAACTGCCGGAGTGGAGCATCGACACTTCGAGCATCGGCGT
CGAGTCCGGCGTCTTCTTCGATGACCTGATCCGCTCGAACCGCGATAAGGAGATGACCAATCCGGGACGCGCCAACAGCT
TCCGTTCCAGCCGGCTCATCCCCGCCGTGGAGTATCTGCAGGGGCAGCGCGCGCGCAGCATGATGATGGCGAAGCTGGCG
GAGGCCACCAGGGACATCGATGTTTACCTGGTGCCTGTGAACCAGGGCGGGGGAGGAGGTGCCGCCGGGCGCGGCGCTGC
TCCGGCCGACGGCGGTCGAGGCGGCCGTGGCGGTGGACCCAATCGCAGCGTGGTAGGCCGCCATTTCGGAATGGCCAACC
TGGCCTGCTACCCGGCACTTAACGTGGTGAACGGCTTTACCGACGCCGGCACGCCTACGAACATCACCTTCTACGCCCGC
CCGTTCGGGGAGGCCGAACTCCTGGCGCTCGGCAAAGCCTACCAGGATGCTACCGGCTTCCATTTGAAGCATCCCGCACT
GGCGTGA

Upstream 100 bases:

>100_bases
AGAAGTTATCGGAGGCCAAGGCGCCGCGTTCCCTCCGCGAATTTACGAGCAACTTTTACGATCGCCAGAAGAGCCTGGGG
TAGAATCGTCTAAACTGCCC

Downstream 100 bases:

>100_bases
GGCAGCCGGTCTACTCGAATCGCAATGCCACACTCGGGTCCACCCGTGTAGCTTGCCGACTGGGAAAGTAGATCGCGAGC
AGCCCAACAACACTAAGCAC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MTPDKMRRRFLACCSGIGLAGTLLPGVLWAQLQQDGAQQVTPEMLKAALGLSGLSFSESDQKSMLQAVNGSLARYDEVRN
LHIPNNVAPPFYFSALTPGMKVNRTREPLRFSTPAVKRPANLEDVAFWPVTHLAQLIKTRQVTSTELTKMYLARLHKYNE
KVNCVVTFLDEVALAQAKQADAEIAAGRYKGALHGIPWGAKDIIAVKGYKTTWGSGAYKEQMLDEEASLVEMLRDAGAVL
LAKLTTGELAQGDQWFGGQTKNPWNIEQGSSGSSAGPASATAGGLVGFGIGSETSGSILSPSARCGVTGLRPTFGRISRY
GVMALSWTQDRMGPLCRYAEDCALVMSVIARPDGRDLSVSEIPFNWNAHLDVRKLRVGYLEGAFEETRDAAVKKIEERTL
EQVKALGIQLVPVKLPEWSIDTSSIGVESGVFFDDLIRSNRDKEMTNPGRANSFRSSRLIPAVEYLQGQRARSMMMAKLA
EATRDIDVYLVPVNQGGGGGAAGRGAAPADGGRGGRGGGPNRSVVGRHFGMANLACYPALNVVNGFTDAGTPTNITFYAR
PFGEAELLALGKAYQDATGFHLKHPALA

Sequences:

>Translated_588_residues
MTPDKMRRRFLACCSGIGLAGTLLPGVLWAQLQQDGAQQVTPEMLKAALGLSGLSFSESDQKSMLQAVNGSLARYDEVRN
LHIPNNVAPPFYFSALTPGMKVNRTREPLRFSTPAVKRPANLEDVAFWPVTHLAQLIKTRQVTSTELTKMYLARLHKYNE
KVNCVVTFLDEVALAQAKQADAEIAAGRYKGALHGIPWGAKDIIAVKGYKTTWGSGAYKEQMLDEEASLVEMLRDAGAVL
LAKLTTGELAQGDQWFGGQTKNPWNIEQGSSGSSAGPASATAGGLVGFGIGSETSGSILSPSARCGVTGLRPTFGRISRY
GVMALSWTQDRMGPLCRYAEDCALVMSVIARPDGRDLSVSEIPFNWNAHLDVRKLRVGYLEGAFEETRDAAVKKIEERTL
EQVKALGIQLVPVKLPEWSIDTSSIGVESGVFFDDLIRSNRDKEMTNPGRANSFRSSRLIPAVEYLQGQRARSMMMAKLA
EATRDIDVYLVPVNQGGGGGAAGRGAAPADGGRGGRGGGPNRSVVGRHFGMANLACYPALNVVNGFTDAGTPTNITFYAR
PFGEAELLALGKAYQDATGFHLKHPALA
>Mature_587_residues
TPDKMRRRFLACCSGIGLAGTLLPGVLWAQLQQDGAQQVTPEMLKAALGLSGLSFSESDQKSMLQAVNGSLARYDEVRNL
HIPNNVAPPFYFSALTPGMKVNRTREPLRFSTPAVKRPANLEDVAFWPVTHLAQLIKTRQVTSTELTKMYLARLHKYNEK
VNCVVTFLDEVALAQAKQADAEIAAGRYKGALHGIPWGAKDIIAVKGYKTTWGSGAYKEQMLDEEASLVEMLRDAGAVLL
AKLTTGELAQGDQWFGGQTKNPWNIEQGSSGSSAGPASATAGGLVGFGIGSETSGSILSPSARCGVTGLRPTFGRISRYG
VMALSWTQDRMGPLCRYAEDCALVMSVIARPDGRDLSVSEIPFNWNAHLDVRKLRVGYLEGAFEETRDAAVKKIEERTLE
QVKALGIQLVPVKLPEWSIDTSSIGVESGVFFDDLIRSNRDKEMTNPGRANSFRSSRLIPAVEYLQGQRARSMMMAKLAE
ATRDIDVYLVPVNQGGGGGAAGRGAAPADGGRGGRGGGPNRSVVGRHFGMANLACYPALNVVNGFTDAGTPTNITFYARP
FGEAELLALGKAYQDATGFHLKHPALA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=25.8, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI195972892, Length=258, Percent_Identity=26.3565891472868, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI166795287, Length=386, Percent_Identity=24.6113989637306, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=267, Percent_Identity=30.7116104868914, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=311, Percent_Identity=28.9389067524116, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=327, Percent_Identity=24.1590214067278, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=310, Percent_Identity=26.7741935483871, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=310, Percent_Identity=26.7741935483871, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=366, Percent_Identity=24.0437158469945, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=290, Percent_Identity=24.8275862068966, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=237, Percent_Identity=29.535864978903, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320448, Length=272, Percent_Identity=29.7794117647059, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=396, Percent_Identity=24.4949494949495, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24644968, Length=250, Percent_Identity=32, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356731, Length=266, Percent_Identity=28.5714285714286, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648435, Length=246, Percent_Identity=31.3008130081301, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24648437, Length=246, Percent_Identity=31.3008130081301, Blast_Score=107, Evalue=3e-23,
Organism=Drosophila melanogaster, GI45550774, Length=246, Percent_Identity=31.3008130081301, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24652985, Length=266, Percent_Identity=26.3157894736842, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI19922090, Length=266, Percent_Identity=26.3157894736842, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24652981, Length=266, Percent_Identity=26.3157894736842, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24652983, Length=266, Percent_Identity=26.3157894736842, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI161078093, Length=335, Percent_Identity=28.3582089552239, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648113, Length=267, Percent_Identity=30.3370786516854, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648441, Length=175, Percent_Identity=31.4285714285714, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648439, Length=175, Percent_Identity=31.4285714285714, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 63243; Mature: 63112

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDKMRRRFLACCSGIGLAGTLLPGVLWAQLQQDGAQQVTPEMLKAALGLSGLSFSESD
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCCCHH
QKSMLQAVNGSLARYDEVRNLHIPNNVAPPFYFSALTPGMKVNRTREPLRFSTPAVKRPA
HHHHHHHHCCCHHHHHHHCCEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
NLEDVAFWPVTHLAQLIKTRQVTSTELTKMYLARLHKYNEKVNCVVTFLDEVALAQAKQA
CCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DAEIAAGRYKGALHGIPWGAKDIIAVKGYKTTWGSGAYKEQMLDEEASLVEMLRDAGAVL
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
LAKLTTGELAQGDQWFGGQTKNPWNIEQGSSGSSAGPASATAGGLVGFGIGSETSGSILS
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
PSARCGVTGLRPTFGRISRYGVMALSWTQDRMGPLCRYAEDCALVMSVIARPDGRDLSVS
CCHHCCCCCCCCHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
EIPFNWNAHLDVRKLRVGYLEGAFEETRDAAVKKIEERTLEQVKALGIQLVPVKLPEWSI
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
DTSSIGVESGVFFDDLIRSNRDKEMTNPGRANSFRSSRLIPAVEYLQGQRARSMMMAKLA
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHH
EATRDIDVYLVPVNQGGGGGAAGRGAAPADGGRGGRGGGPNRSVVGRHFGMANLACYPAL
HHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHH
NVVNGFTDAGTPTNITFYARPFGEAELLALGKAYQDATGFHLKHPALA
HHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TPDKMRRRFLACCSGIGLAGTLLPGVLWAQLQQDGAQQVTPEMLKAALGLSGLSFSESD
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHCCCCCCCCCHH
QKSMLQAVNGSLARYDEVRNLHIPNNVAPPFYFSALTPGMKVNRTREPLRFSTPAVKRPA
HHHHHHHHCCCHHHHHHHCCEECCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
NLEDVAFWPVTHLAQLIKTRQVTSTELTKMYLARLHKYNEKVNCVVTFLDEVALAQAKQA
CCCCEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
DAEIAAGRYKGALHGIPWGAKDIIAVKGYKTTWGSGAYKEQMLDEEASLVEMLRDAGAVL
HHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEE
LAKLTTGELAQGDQWFGGQTKNPWNIEQGSSGSSAGPASATAGGLVGFGIGSETSGSILS
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
PSARCGVTGLRPTFGRISRYGVMALSWTQDRMGPLCRYAEDCALVMSVIARPDGRDLSVS
CCHHCCCCCCCCHHHHHHHCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
EIPFNWNAHLDVRKLRVGYLEGAFEETRDAAVKKIEERTLEQVKALGIQLVPVKLPEWSI
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
DTSSIGVESGVFFDDLIRSNRDKEMTNPGRANSFRSSRLIPAVEYLQGQRARSMMMAKLA
CCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHH
EATRDIDVYLVPVNQGGGGGAAGRGAAPADGGRGGRGGGPNRSVVGRHFGMANLACYPAL
HHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHH
NVVNGFTDAGTPTNITFYARPFGEAELLALGKAYQDATGFHLKHPALA
HHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA