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The map label for this gene is lacZ [H]

Identifier: 116623989

GI number: 116623989

Start: 6182008

End: 6184494

Strand: Reverse

Name: lacZ [H]

Synonym: Acid_4901

Alternate gene names: 116623989

Gene position: 6184494-6182008 (Counterclockwise)

Preceding gene: 116623990

Following gene: 116623985

Centisome position: 62.06

GC content: 63.73

Gene sequence:

>2487_bases
ATGAACAAGATAGTCGCGCCTGCAGCCGTTCTGTGCGCGATTCTGACATGGCCCGCCGCTCCCGTTCGAGCCTCGGGCGC
TACCGCGGTCACGGTTTCCGGCGAACGCCGGCAGAGCTTCAACCAGGGCTGGCGGTTCTTCCGCGGCGATGCGGAAGGCG
CCGAAGTCCCGGGGTTTAACGACGCGGCGTGGACCGCGGTCAGGCTGCCGCACGACTGGGCGATTGCGGGGCCGTTCGAT
TCCAAGCTCAATCCGCATACGGGCGCGCTGCCGATTTCGGGCACGGGATGGTACCGGAAGGCCTTCACACTGCCGGCGAG
CGCCGCGGGCCGCGTGTTCTCGATCGAGTTCGACGGGGCGATGTCGAACGCGCGGGTCTGGCTCAACGGCAAGGAACTGG
GGAGCCGTCCTTACGGCTACATCGGCTTCGGTTTCGATCTCACGCCACCGCTGCATTTCGGCGCGGAAGAGAATGTGCTT
GCAGTTAGACTGAAGCCGGAGGAGCGCTCGTCGCGCTGGTACCCGGGGGCGGGAATCTATCGCAACGTCTGGCTGGATAT
TACGGGACCGGTGCGAGTGGCACGGTGGGGCACGTACGTCACGACGCCGGAGGCCGGCACGGCCAAGGCCACGGTGGCGG
CGAAGACCGAAGTCGAGAACCGCACGGCGAGCGAGGTGCGGGTCGTGCTGCAGACTTCGCTGGTGGATAGCCGTGGAAAG
ACGGTGGCGAGCGAACGGCGCGAGGCTGCGATTGCGGCGGGCGGCGCGCAGACCATCGACGCGAACCTGGCGGTCGCGAG
GCCCAGGCTTTGGGACACGGAGTCTCCGTATCTGTATACGGTGGTCAGCGAGGTGATGGAGGGGAGGCGGGTGCTGGACC
GGTATGAAACGCCGGTTGGCATTCGCGCTATCGCGTTCGACAAGAACAAGGGATTTCAGTTGAACGGGCGCTCGATGAAG
CTGCAGGGCGTCTGTCTGCACCACGACCTGGGAGCGCTGGGCGCGGCGGTCAACCGGCGCGCGATCGAGCGGCAGCTGGA
GATTATGAAGGGCGCAGGCGTGAATGCGATCCGCACGAGTCACAATCCGCCGGCCCCGGAACTGCTCGAACTGGCCGACC
GCATGGGGCTGCTGGTGATCGACGAAGCCTTCGATATGTGGCGGATTCCGAAGGTGCCGAATGGCTATGCAAAGTACTTC
GACGAGTGGAGCGAGCGCGACATTCGCGATATGGTGCGGCGCGATCGGAACCACCCGAGCGTGATTCTATGGAGCATCGG
TAACGAGGTGGGCGAGCAGGGGCGACCGGACGGGTGGCAGGTGGCGAAGCGGCTGGTGGACGTGGTGCATGCCGAAGATC
GCACGCGCCCGACCACGGCAGCGTTCGACAATTGGGTCGGGGCGATCAAGAATAGGGTCGCCGAGCAGGTGGAGGTGCAG
GGGTTCAATTACAAGCCCAACCACTACGAGGAGATTCTGAAGGGCCATCCCGATTGGATGATCCTGGCGACGGAGAGCAG
TTCCACGGTGAGTTCGCGCGGGATCTATCATGTTCCACTGGAGAATTATCCGAAGCACGCTTCGCTACAGATCACGAGTT
ATGACGTGATGGCTCCGGCTTGGGCGACGCTGCCCGATGTGGAGTTCAGTTACCAGGACAAATTGCCGAATGTGCTCGGC
GAATTCGTTTGGACCGGGTTCGACTATATCGGCGAGCCTACGCCGTTCTTCGGTGGGAGAAGTTCGGCGGACGACTGGCC
GGCGCGCAGTTCGTATTTCGGGATGGTGGACCTGGCCGGTTTTCCGAAGGATCGCTACTATCTTTACCAGAGTGTGTGGA
CCACGAAACCGATGGTGCATGTGCTTCCGCACTGGAACTGGCAGGGCAGGGAAGGGGAGAAGATTCCGGTGATGGTGTAC
AGCAATGGCGATGAGGTGGAGCTGATCCTGAATGGCAAATCGCTGGGACGGAAGCGGCGCTTCGGCGAGGCGGTGGAACT
GCCTGTGGGCGGGAACGTGAGCGCCGACAAGAAGTTCGTGAGTAAGTACCGGCTGACGTGGGAGGTGCCGTACGCGGCGG
GGACACTGAGGGCCGTGAGTTATCGGGGCGGCAAGCAGGTGGCGGTGGATGAGGTTCGTACCGCGGGCGCGCCGGCGCGA
CTCCGGCTGATTGCGGACCGGTCCACGATTCACGGAGACGGCGACGATCTGGCGTTTGTCACGGTGCGGATAGAGGATAA
GGACGGGAATCTCTGCCCGGGGGCGGATAATTTGATTCGGTTTCAGGTGACCGGCGCGGGCGGGATCCGCGCGGTCGATA
ACGGGAATGCGGCATCCACGGAGCCGTTCCAGGCGGAGTACCGGAAGGCCTTCAGCGGACTGGCGCTGCTGATTGTCGAG
GGCGCGAAAGCGGGGAAGATTCAGGTGGCGGCGACGGCTGAAGGGCTGGTCGCGGGGGTGACGGAGATAACGGCGATCGC
TCCCTGA

Upstream 100 bases:

>100_bases
TGGCTCCCGCGCCGTTGTGCAACTGGAGCGACCAACATCCTGAATGGTGGTCGTCGTCGGGCTGGCAGCTGATGTAAGTG
CAGGGCGGTACATTGTAGGG

Downstream 100 bases:

>100_bases
CTGTCGCGGTTCGCGGGCGGTTACGGTGTCACACCGACGAGCTTGGAGGTTTGGAGCCAGGTTTTGTCGGGGTAATAGGC
GAGGACGAGGCTTCCGCCCT

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 828; Mature: 828

Protein sequence:

>828_residues
MNKIVAPAAVLCAILTWPAAPVRASGATAVTVSGERRQSFNQGWRFFRGDAEGAEVPGFNDAAWTAVRLPHDWAIAGPFD
SKLNPHTGALPISGTGWYRKAFTLPASAAGRVFSIEFDGAMSNARVWLNGKELGSRPYGYIGFGFDLTPPLHFGAEENVL
AVRLKPEERSSRWYPGAGIYRNVWLDITGPVRVARWGTYVTTPEAGTAKATVAAKTEVENRTASEVRVVLQTSLVDSRGK
TVASERREAAIAAGGAQTIDANLAVARPRLWDTESPYLYTVVSEVMEGRRVLDRYETPVGIRAIAFDKNKGFQLNGRSMK
LQGVCLHHDLGALGAAVNRRAIERQLEIMKGAGVNAIRTSHNPPAPELLELADRMGLLVIDEAFDMWRIPKVPNGYAKYF
DEWSERDIRDMVRRDRNHPSVILWSIGNEVGEQGRPDGWQVAKRLVDVVHAEDRTRPTTAAFDNWVGAIKNRVAEQVEVQ
GFNYKPNHYEEILKGHPDWMILATESSSTVSSRGIYHVPLENYPKHASLQITSYDVMAPAWATLPDVEFSYQDKLPNVLG
EFVWTGFDYIGEPTPFFGGRSSADDWPARSSYFGMVDLAGFPKDRYYLYQSVWTTKPMVHVLPHWNWQGREGEKIPVMVY
SNGDEVELILNGKSLGRKRRFGEAVELPVGGNVSADKKFVSKYRLTWEVPYAAGTLRAVSYRGGKQVAVDEVRTAGAPAR
LRLIADRSTIHGDGDDLAFVTVRIEDKDGNLCPGADNLIRFQVTGAGGIRAVDNGNAASTEPFQAEYRKAFSGLALLIVE
GAKAGKIQVAATAEGLVAGVTEITAIAP

Sequences:

>Translated_828_residues
MNKIVAPAAVLCAILTWPAAPVRASGATAVTVSGERRQSFNQGWRFFRGDAEGAEVPGFNDAAWTAVRLPHDWAIAGPFD
SKLNPHTGALPISGTGWYRKAFTLPASAAGRVFSIEFDGAMSNARVWLNGKELGSRPYGYIGFGFDLTPPLHFGAEENVL
AVRLKPEERSSRWYPGAGIYRNVWLDITGPVRVARWGTYVTTPEAGTAKATVAAKTEVENRTASEVRVVLQTSLVDSRGK
TVASERREAAIAAGGAQTIDANLAVARPRLWDTESPYLYTVVSEVMEGRRVLDRYETPVGIRAIAFDKNKGFQLNGRSMK
LQGVCLHHDLGALGAAVNRRAIERQLEIMKGAGVNAIRTSHNPPAPELLELADRMGLLVIDEAFDMWRIPKVPNGYAKYF
DEWSERDIRDMVRRDRNHPSVILWSIGNEVGEQGRPDGWQVAKRLVDVVHAEDRTRPTTAAFDNWVGAIKNRVAEQVEVQ
GFNYKPNHYEEILKGHPDWMILATESSSTVSSRGIYHVPLENYPKHASLQITSYDVMAPAWATLPDVEFSYQDKLPNVLG
EFVWTGFDYIGEPTPFFGGRSSADDWPARSSYFGMVDLAGFPKDRYYLYQSVWTTKPMVHVLPHWNWQGREGEKIPVMVY
SNGDEVELILNGKSLGRKRRFGEAVELPVGGNVSADKKFVSKYRLTWEVPYAAGTLRAVSYRGGKQVAVDEVRTAGAPAR
LRLIADRSTIHGDGDDLAFVTVRIEDKDGNLCPGADNLIRFQVTGAGGIRAVDNGNAASTEPFQAEYRKAFSGLALLIVE
GAKAGKIQVAATAEGLVAGVTEITAIAP
>Mature_828_residues
MNKIVAPAAVLCAILTWPAAPVRASGATAVTVSGERRQSFNQGWRFFRGDAEGAEVPGFNDAAWTAVRLPHDWAIAGPFD
SKLNPHTGALPISGTGWYRKAFTLPASAAGRVFSIEFDGAMSNARVWLNGKELGSRPYGYIGFGFDLTPPLHFGAEENVL
AVRLKPEERSSRWYPGAGIYRNVWLDITGPVRVARWGTYVTTPEAGTAKATVAAKTEVENRTASEVRVVLQTSLVDSRGK
TVASERREAAIAAGGAQTIDANLAVARPRLWDTESPYLYTVVSEVMEGRRVLDRYETPVGIRAIAFDKNKGFQLNGRSMK
LQGVCLHHDLGALGAAVNRRAIERQLEIMKGAGVNAIRTSHNPPAPELLELADRMGLLVIDEAFDMWRIPKVPNGYAKYF
DEWSERDIRDMVRRDRNHPSVILWSIGNEVGEQGRPDGWQVAKRLVDVVHAEDRTRPTTAAFDNWVGAIKNRVAEQVEVQ
GFNYKPNHYEEILKGHPDWMILATESSSTVSSRGIYHVPLENYPKHASLQITSYDVMAPAWATLPDVEFSYQDKLPNVLG
EFVWTGFDYIGEPTPFFGGRSSADDWPARSSYFGMVDLAGFPKDRYYLYQSVWTTKPMVHVLPHWNWQGREGEKIPVMVY
SNGDEVELILNGKSLGRKRRFGEAVELPVGGNVSADKKFVSKYRLTWEVPYAAGTLRAVSYRGGKQVAVDEVRTAGAPAR
LRLIADRSTIHGDGDDLAFVTVRIEDKDGNLCPGADNLIRFQVTGAGGIRAVDNGNAASTEPFQAEYRKAFSGLALLIVE
GAKAGKIQVAATAEGLVAGVTEITAIAP

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=526, Percent_Identity=24.1444866920152, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI48994920, Length=396, Percent_Identity=28.7878787878788, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1787903, Length=417, Percent_Identity=25.8992805755396, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1786539, Length=409, Percent_Identity=29.5843520782396, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17510825, Length=446, Percent_Identity=26.0089686098655, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24651745, Length=521, Percent_Identity=25.7197696737044, Blast_Score=133, Evalue=6e-31,
Organism=Drosophila melanogaster, GI62471735, Length=418, Percent_Identity=26.3157894736842, Blast_Score=128, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19922582, Length=418, Percent_Identity=26.3157894736842, Blast_Score=128, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24655438, Length=418, Percent_Identity=26.3157894736842, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 91004; Mature: 91004

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIVAPAAVLCAILTWPAAPVRASGATAVTVSGERRQSFNQGWRFFRGDAEGAEVPGFN
CCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCEEEECCCCCCCCCCCC
DAAWTAVRLPHDWAIAGPFDSKLNPHTGALPISGTGWYRKAFTLPASAAGRVFSIEFDGA
CCCEEEEECCCCEEEECCCCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCEEEEEECCC
MSNARVWLNGKELGSRPYGYIGFGFDLTPPLHFGAEENVLAVRLKPEERSSRWYPGAGIY
CCCCEEEECCHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCE
RNVWLDITGPVRVARWGTYVTTPEAGTAKATVAAKTEVENRTASEVRVVLQTSLVDSRGK
EEEEEEECCCEEEEECCCEEECCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHCCCCC
TVASERREAAIAAGGAQTIDANLAVARPRLWDTESPYLYTVVSEVMEGRRVLDRYETPVG
HHHHHHHHHEEECCCCEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IRAIAFDKNKGFQLNGRSMKLQGVCLHHDLGALGAAVNRRAIERQLEIMKGAGVNAIRTS
EEEEEEECCCCEEECCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
HNPPAPELLELADRMGLLVIDEAFDMWRIPKVPNGYAKYFDEWSERDIRDMVRRDRNHPS
CCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCC
VILWSIGNEVGEQGRPDGWQVAKRLVDVVHAEDRTRPTTAAFDNWVGAIKNRVAEQVEVQ
EEEECCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEE
GFNYKPNHYEEILKGHPDWMILATESSSTVSSRGIYHVPLENYPKHASLQITSYDVMAPA
CCCCCCHHHHHHHCCCCCEEEEEECCCCCHHCCCEEECCCCCCCCCCEEEEEEEEEECCC
WATLPDVEFSYQDKLPNVLGEFVWTGFDYIGEPTPFFGGRSSADDWPARSSYFGMVDLAG
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
FPKDRYYLYQSVWTTKPMVHVLPHWNWQGREGEKIPVMVYSNGDEVELILNGKSLGRKRR
CCCCCEEEEEHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCHHHCHHHH
FGEAVELPVGGNVSADKKFVSKYRLTWEVPYAAGTLRAVSYRGGKQVAVDEVRTAGAPAR
CCCEEECCCCCCCCHHHHHHHHEEEEEECCCCCCCEEEEEECCCCEEEHHHHHHCCCCEE
LRLIADRSTIHGDGDDLAFVTVRIEDKDGNLCPGADNLIRFQVTGAGGIRAVDNGNAAST
EEEEECCCEECCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCC
EPFQAEYRKAFSGLALLIVEGAKAGKIQVAATAEGLVAGVTEITAIAP
CCHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCCHHHHHHHHEEECC
>Mature Secondary Structure
MNKIVAPAAVLCAILTWPAAPVRASGATAVTVSGERRQSFNQGWRFFRGDAEGAEVPGFN
CCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHCCEEEECCCCCCCCCCCC
DAAWTAVRLPHDWAIAGPFDSKLNPHTGALPISGTGWYRKAFTLPASAAGRVFSIEFDGA
CCCEEEEECCCCEEEECCCCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCEEEEEECCC
MSNARVWLNGKELGSRPYGYIGFGFDLTPPLHFGAEENVLAVRLKPEERSSRWYPGAGIY
CCCCEEEECCHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCE
RNVWLDITGPVRVARWGTYVTTPEAGTAKATVAAKTEVENRTASEVRVVLQTSLVDSRGK
EEEEEEECCCEEEEECCCEEECCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHCCCCC
TVASERREAAIAAGGAQTIDANLAVARPRLWDTESPYLYTVVSEVMEGRRVLDRYETPVG
HHHHHHHHHEEECCCCEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
IRAIAFDKNKGFQLNGRSMKLQGVCLHHDLGALGAAVNRRAIERQLEIMKGAGVNAIRTS
EEEEEEECCCCEEECCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
HNPPAPELLELADRMGLLVIDEAFDMWRIPKVPNGYAKYFDEWSERDIRDMVRRDRNHPS
CCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCC
VILWSIGNEVGEQGRPDGWQVAKRLVDVVHAEDRTRPTTAAFDNWVGAIKNRVAEQVEVQ
EEEECCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEE
GFNYKPNHYEEILKGHPDWMILATESSSTVSSRGIYHVPLENYPKHASLQITSYDVMAPA
CCCCCCHHHHHHHCCCCCEEEEEECCCCCHHCCCEEECCCCCCCCCCEEEEEEEEEECCC
WATLPDVEFSYQDKLPNVLGEFVWTGFDYIGEPTPFFGGRSSADDWPARSSYFGMVDLAG
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
FPKDRYYLYQSVWTTKPMVHVLPHWNWQGREGEKIPVMVYSNGDEVELILNGKSLGRKRR
CCCCCEEEEEHHHCCCCEEEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCHHHCHHHH
FGEAVELPVGGNVSADKKFVSKYRLTWEVPYAAGTLRAVSYRGGKQVAVDEVRTAGAPAR
CCCEEECCCCCCCCHHHHHHHHEEEEEECCCCCCCEEEEEECCCCEEEHHHHHHCCCCEE
LRLIADRSTIHGDGDDLAFVTVRIEDKDGNLCPGADNLIRFQVTGAGGIRAVDNGNAAST
EEEEECCCEECCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCC
EPFQAEYRKAFSGLALLIVEGAKAGKIQVAATAEGLVAGVTEITAIAP
CCHHHHHHHHHCCEEEEEEECCCCCEEEEEEECCCHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA