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The map label for this gene is ltaA [H]

Identifier: 116623892

GI number: 116623892

Start: 6076107

End: 6077213

Strand: Direct

Name: ltaA [H]

Synonym: Acid_4804

Alternate gene names: 116623892

Gene position: 6076107-6077213 (Clockwise)

Preceding gene: 116623891

Following gene: 116623898

Centisome position: 60.97

GC content: 64.59

Gene sequence:

>1107_bases
ATGCAACGCAGGAGTTTTCTCGCAGCGCCGCTCGCGGCATGGCAACTGAAGGCGGCTGAACCCGAGAAGCGCATTTACGC
GTTCGGCGACGGCATACTGCACACTCCCGCCGAGTATTCGCAGCTGCTCGCGACCCTCACCGCCAAAGGAGAGGTCACGC
CCGACAACTACTCACTCGGCGGCGTGGTCGAGCAGGTGGAGACCCGTATGGCCGCGCTGCTCGGTAAGGAAGCGGCGGTC
TGGCTGTCCACCGGTACGCTGGCGAACCATCTGGCGGTGCGCCTGCTCGCCGGGAGCCGGCGCCGCGTTCTGGTGCAGGC
CGAGAGCCACCTGATGAACGATTGCGGCGATTGCGCCGAGGTATTGAGCGGCCTCCACCTGGTGCCGCTCGCCCCCGGCA
AAGCGACCTTCACCTTAGCCGATGTAGAGCGCGCCGCGTACGAAGCCTCGACCGGCCGCGTCAACACGCCGATCGGCGCC
ATCCAGATCGAGTCTCCGGTCCGCCGTAAGCAGGGCGAGCGCTTCGATTTCGAGGAGATGAAGAAGATCGCGCAGTGGGC
CCGCGCGCACAGTGTGGGCCTGCACCTGGATGGTGCGAGGCTCTTCCTCGAGAGCGGCTACACCCGGCGCCCGGTGAAGG
AATACACGGCTCTCTTCGATACCGTCTACGTCTCCATGTACAAGTACTTCAACGCCGCCTCCGGCGCGATTCTCGCAGGG
CCCAAGGCCCTCCTGGCGGATCTCTTTCAAACCCGCCGCATGTTCGGCGGAGGCCTGCCCGAAGTCTGGCCGTTCGCCGC
CGTCGCGCTGCACTACGTGAATGGATTCGAACAGCGCTTCCGCGGCAGCGTGGAAACCGCGGAACGCGTGATCGCGGCCT
TGCGCGCCGATTCGAATTTCGGAGTCGAGGCCATTCCGAACGGCACGAACATCTTCCGCATGCGCGTCTTCAACGTCAAT
GCGCCGGTCTACAAACTGCGCCTCGAAGATACCGGCATCAGCGTGCACGATCCCGTGGGAGACTGGTTCGCGATGCAGGT
CAACGAAACCTGGAATCGCCTGCCGCCAACGGAAATTTACTCCCGCTTCCGGCAGGCGCTCGGCTGA

Upstream 100 bases:

>100_bases
GGGTACGGCCCCGAAGGACGAGTTCGTCTTCGGCTTTTCCTCACTCACCGAGCGCGCCATTCGCGAGGGCATCCGGCGGC
TGGTATGATGAATTCCCAGA

Downstream 100 bases:

>100_bases
ACCAGCTAGATGGAACGCGAGCCCATGGTCACGATATCTTCCACCTGCATCGCTTCCTTCACCACGAACGGTTCGCCGTA
TTTGACGCCGATCTGATTCC

Product: L-threonine aldolase

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MQRRSFLAAPLAAWQLKAAEPEKRIYAFGDGILHTPAEYSQLLATLTAKGEVTPDNYSLGGVVEQVETRMAALLGKEAAV
WLSTGTLANHLAVRLLAGSRRRVLVQAESHLMNDCGDCAEVLSGLHLVPLAPGKATFTLADVERAAYEASTGRVNTPIGA
IQIESPVRRKQGERFDFEEMKKIAQWARAHSVGLHLDGARLFLESGYTRRPVKEYTALFDTVYVSMYKYFNAASGAILAG
PKALLADLFQTRRMFGGGLPEVWPFAAVALHYVNGFEQRFRGSVETAERVIAALRADSNFGVEAIPNGTNIFRMRVFNVN
APVYKLRLEDTGISVHDPVGDWFAMQVNETWNRLPPTEIYSRFRQALG

Sequences:

>Translated_368_residues
MQRRSFLAAPLAAWQLKAAEPEKRIYAFGDGILHTPAEYSQLLATLTAKGEVTPDNYSLGGVVEQVETRMAALLGKEAAV
WLSTGTLANHLAVRLLAGSRRRVLVQAESHLMNDCGDCAEVLSGLHLVPLAPGKATFTLADVERAAYEASTGRVNTPIGA
IQIESPVRRKQGERFDFEEMKKIAQWARAHSVGLHLDGARLFLESGYTRRPVKEYTALFDTVYVSMYKYFNAASGAILAG
PKALLADLFQTRRMFGGGLPEVWPFAAVALHYVNGFEQRFRGSVETAERVIAALRADSNFGVEAIPNGTNIFRMRVFNVN
APVYKLRLEDTGISVHDPVGDWFAMQVNETWNRLPPTEIYSRFRQALG
>Mature_368_residues
MQRRSFLAAPLAAWQLKAAEPEKRIYAFGDGILHTPAEYSQLLATLTAKGEVTPDNYSLGGVVEQVETRMAALLGKEAAV
WLSTGTLANHLAVRLLAGSRRRVLVQAESHLMNDCGDCAEVLSGLHLVPLAPGKATFTLADVERAAYEASTGRVNTPIGA
IQIESPVRRKQGERFDFEEMKKIAQWARAHSVGLHLDGARLFLESGYTRRPVKEYTALFDTVYVSMYKYFNAASGAILAG
PKALLADLFQTRRMFGGGLPEVWPFAAVALHYVNGFEQRFRGSVETAERVIAALRADSNFGVEAIPNGTNIFRMRVFNVN
APVYKLRLEDTGISVHDPVGDWFAMQVNETWNRLPPTEIYSRFRQALG

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=274, Percent_Identity=25.5474452554745, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI193206524, Length=213, Percent_Identity=26.2910798122066, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193206522, Length=213, Percent_Identity=26.2910798122066, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193206520, Length=213, Percent_Identity=26.2910798122066, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320789, Length=215, Percent_Identity=27.906976744186, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21355439, Length=229, Percent_Identity=28.82096069869, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 40661; Mature: 40661

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRSFLAAPLAAWQLKAAEPEKRIYAFGDGILHTPAEYSQLLATLTAKGEVTPDNYSLG
CCCCHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHH
GVVEQVETRMAALLGKEAAVWLSTGTLANHLAVRLLAGSRRRVLVQAESHLMNDCGDCAE
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHH
VLSGLHLVPLAPGKATFTLADVERAAYEASTGRVNTPIGAIQIESPVRRKQGERFDFEEM
HHCCCEEEEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHCCCCCCCHHHH
KKIAQWARAHSVGLHLDGARLFLESGYTRRPVKEYTALFDTVYVSMYKYFNAASGAILAG
HHHHHHHHHHHCCEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PKALLADLFQTRRMFGGGLPEVWPFAAVALHYVNGFEQRFRGSVETAERVIAALRADSNF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
GVEAIPNGTNIFRMRVFNVNAPVYKLRLEDTGISVHDPVGDWFAMQVNETWNRLPPTEIY
CCEECCCCCCEEEEEEEECCCCEEEEEEECCCCEEECCCCCEEEEEECCHHCCCCCHHHH
SRFRQALG
HHHHHHCC
>Mature Secondary Structure
MQRRSFLAAPLAAWQLKAAEPEKRIYAFGDGILHTPAEYSQLLATLTAKGEVTPDNYSLG
CCCCHHHHHHHHHHEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHH
GVVEQVETRMAALLGKEAAVWLSTGTLANHLAVRLLAGSRRRVLVQAESHLMNDCGDCAE
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHH
VLSGLHLVPLAPGKATFTLADVERAAYEASTGRVNTPIGAIQIESPVRRKQGERFDFEEM
HHCCCEEEEECCCCCEEEHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHCCCCCCCHHHH
KKIAQWARAHSVGLHLDGARLFLESGYTRRPVKEYTALFDTVYVSMYKYFNAASGAILAG
HHHHHHHHHHHCCEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
PKALLADLFQTRRMFGGGLPEVWPFAAVALHYVNGFEQRFRGSVETAERVIAALRADSNF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
GVEAIPNGTNIFRMRVFNVNAPVYKLRLEDTGISVHDPVGDWFAMQVNETWNRLPPTEIY
CCEECCCCCCEEEEEEEECCCCEEEEEEECCCCEEECCCCCEEEEEECCHHCCCCCHHHH
SRFRQALG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]