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The map label for this gene is mdeA [H]

Identifier: 116623882

GI number: 116623882

Start: 6060083

End: 6061330

Strand: Direct

Name: mdeA [H]

Synonym: Acid_4794

Alternate gene names: 116623882

Gene position: 6060083-6061330 (Clockwise)

Preceding gene: 116623881

Following gene: 116623886

Centisome position: 60.81

GC content: 62.58

Gene sequence:

>1248_bases
ATGTCCGACCAAGATCATGTTCACGGGGTGGTGCGGCCGCGCACAGCGGTATTGACGCGCGGGTTCGATCCCAGATTGTC
GGTGGGTTCGGCGCGCCCGGCGGTATTCCGGAGTTCCACCTACGTATTTGCGAATCCCGAGGAGGCCGAACACGCCTTCG
CGATCGTTACCGGCAAAATCAAGCCGGAGCACGGCGGACGTGCCGATCTCATCTATTCGCGCTTCTCCCACCCGAACGCT
GAGATTCTGGAAGACCAGATTGTGCCGCTTGAAGCCGGCGCAGCCTACTCCGCGGTCTTCAATTCCGGCATGGCGGCGAT
CCAAACGGCCTTCTTCGCCTACGCGCGCCCCGACAGCACCATGGTCTACACCACGCCCCTTTACGGCGGCACCACAGGCC
TGATCCACAAATTCATGAAGCCGCTCGGCGTCACCGGAATTCCCGTGACCTCCGGCAAGACCGAAGCCATCGAAGAAGCC
ATCCGCGGCGCCCGGAACTGTTGCATGGTATACCTCGAAACTCCGGCCAACCCCACCATGATCATGACCGATATCGAGCG
CGCCGCCGAAGCCGCCGCCAAACACCCCGATAAGCCGGTCGTCATGGTGGACAATACCTTCCTGGGACCCTCCTTCCAGC
ATCCCCTGCTGCTCGGCGCCAACATCTGCCTGTACTCCGCCACCAAGTATCTCTCCGGCTACAGCGACATGCTGGGCGGC
GTCGCCATCACCGACAGCGCCGACATCGTCCAGAAACTGCGCAGCGTGCGCAGCATGTTCGGCAACATCCTGCAGCCCGA
CGAATGCTGGATGCTGGACAGCCGCCTCTCCACCGTCGCCCTGCGCATGAACCGGCAGAGCAAGAACGCCCAGCGCCTTG
CCGAGGCGCTCCATGGCCACCGCCTGTTGAGCCAGGTGATCTATCCCACGCTGTTCACCGACCCCGAACAGAAACGCATC
TTCGAGAAGCAGTGCGATTTCCCGGGCGGCATGTTCTCCATCGAATTTAAGGGCGGCAAGGAAGCGGCATTCCAATTCCT
CCGCCACGTGCGCATCGCGCGCAATGCGGTTTCTCTCGGCAGCGTCGAATCCCTGACCTGCCATCCGAAATCCACCACGC
ACTCCGGAATGAGCGCCGAAGAGCTGGCCGAAAGCGGCGTCACCGACGGCCTCGTCCGCATCTCCGTCGGCATCGAGGAC
TGGCGCGACTTGCTCGCTGATTTCGAACAGGCCCTCGCCGCCGTCTAG

Upstream 100 bases:

>100_bases
CGCGGCTCGGCAAACGCCGGCGTCATAAAAGCCACAAAGCACCACCCTCCCGTTGTGCCCAGGTGCCCCGTTTGGCGCTA
CACTCGGTTTGGGAGGTTCC

Downstream 100 bases:

>100_bases
CCCGGAACTCTCAGCCCCTTCTCGCGCACCAGCAATCGTCCGCGAACCGCGACCGTTAGGGAGCGATCTCTTCCACCTTC
ACTCGTGCCGCAGCGCAATC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MSDQDHVHGVVRPRTAVLTRGFDPRLSVGSARPAVFRSSTYVFANPEEAEHAFAIVTGKIKPEHGGRADLIYSRFSHPNA
EILEDQIVPLEAGAAYSAVFNSGMAAIQTAFFAYARPDSTMVYTTPLYGGTTGLIHKFMKPLGVTGIPVTSGKTEAIEEA
IRGARNCCMVYLETPANPTMIMTDIERAAEAAAKHPDKPVVMVDNTFLGPSFQHPLLLGANICLYSATKYLSGYSDMLGG
VAITDSADIVQKLRSVRSMFGNILQPDECWMLDSRLSTVALRMNRQSKNAQRLAEALHGHRLLSQVIYPTLFTDPEQKRI
FEKQCDFPGGMFSIEFKGGKEAAFQFLRHVRIARNAVSLGSVESLTCHPKSTTHSGMSAEELAESGVTDGLVRISVGIED
WRDLLADFEQALAAV

Sequences:

>Translated_415_residues
MSDQDHVHGVVRPRTAVLTRGFDPRLSVGSARPAVFRSSTYVFANPEEAEHAFAIVTGKIKPEHGGRADLIYSRFSHPNA
EILEDQIVPLEAGAAYSAVFNSGMAAIQTAFFAYARPDSTMVYTTPLYGGTTGLIHKFMKPLGVTGIPVTSGKTEAIEEA
IRGARNCCMVYLETPANPTMIMTDIERAAEAAAKHPDKPVVMVDNTFLGPSFQHPLLLGANICLYSATKYLSGYSDMLGG
VAITDSADIVQKLRSVRSMFGNILQPDECWMLDSRLSTVALRMNRQSKNAQRLAEALHGHRLLSQVIYPTLFTDPEQKRI
FEKQCDFPGGMFSIEFKGGKEAAFQFLRHVRIARNAVSLGSVESLTCHPKSTTHSGMSAEELAESGVTDGLVRISVGIED
WRDLLADFEQALAAV
>Mature_414_residues
SDQDHVHGVVRPRTAVLTRGFDPRLSVGSARPAVFRSSTYVFANPEEAEHAFAIVTGKIKPEHGGRADLIYSRFSHPNAE
ILEDQIVPLEAGAAYSAVFNSGMAAIQTAFFAYARPDSTMVYTTPLYGGTTGLIHKFMKPLGVTGIPVTSGKTEAIEEAI
RGARNCCMVYLETPANPTMIMTDIERAAEAAAKHPDKPVVMVDNTFLGPSFQHPLLLGANICLYSATKYLSGYSDMLGGV
AITDSADIVQKLRSVRSMFGNILQPDECWMLDSRLSTVALRMNRQSKNAQRLAEALHGHRLLSQVIYPTLFTDPEQKRIF
EKQCDFPGGMFSIEFKGGKEAAFQFLRHVRIARNAVSLGSVESLTCHPKSTTHSGMSAEELAESGVTDGLVRISVGIEDW
RDLLADFEQALAAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=350, Percent_Identity=34.8571428571429, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI299473759, Length=266, Percent_Identity=37.218045112782, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI25453487, Length=350, Percent_Identity=28.8571428571429, Blast_Score=125, Evalue=5e-29,
Organism=Escherichia coli, GI1790375, Length=402, Percent_Identity=32.3383084577114, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789383, Length=407, Percent_Identity=25.0614250614251, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI71980708, Length=404, Percent_Identity=33.9108910891089, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=389, Percent_Identity=33.9331619537275, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=402, Percent_Identity=34.0796019900498, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319307, Length=345, Percent_Identity=37.6811594202899, Blast_Score=199, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=426, Percent_Identity=29.5774647887324, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321904, Length=345, Percent_Identity=29.2753623188406, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321254, Length=366, Percent_Identity=29.5081967213115, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=319, Percent_Identity=24.4514106583072, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6322590, Length=346, Percent_Identity=23.9884393063584, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922554, Length=350, Percent_Identity=34.8571428571429, Blast_Score=197, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45289; Mature: 45158

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQDHVHGVVRPRTAVLTRGFDPRLSVGSARPAVFRSSTYVFANPEEAEHAFAIVTGKI
CCCCCCCCEECCCCHHHHCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCEEEEEEEEE
KPEHGGRADLIYSRFSHPNAEILEDQIVPLEAGAAYSAVFNSGMAAIQTAFFAYARPDST
CCCCCCCHHHHHHHCCCCCHHHHHCCCCEECCCCHHHHHHHCCHHHHHHHHHHEECCCCE
MVYTTPLYGGTTGLIHKFMKPLGVTGIPVTSGKTEAIEEAIRGARNCCMVYLETPANPTM
EEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEE
IMTDIERAAEAAAKHPDKPVVMVDNTFLGPSFQHPLLLGANICLYSATKYLSGYSDMLGG
EEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHCCHHHHHCC
VAITDSADIVQKLRSVRSMFGNILQPDECWMLDSRLSTVALRMNRQSKNAQRLAEALHGH
EEEECCHHHHHHHHHHHHHHCCCCCCCHHEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RLLSQVIYPTLFTDPEQKRIFEKQCDFPGGMFSIEFKGGKEAAFQFLRHVRIARNAVSLG
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCC
SVESLTCHPKSTTHSGMSAEELAESGVTDGLVRISVGIEDWRDLLADFEQALAAV
CCCEEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDQDHVHGVVRPRTAVLTRGFDPRLSVGSARPAVFRSSTYVFANPEEAEHAFAIVTGKI
CCCCCCCEECCCCHHHHCCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCEEEEEEEEE
KPEHGGRADLIYSRFSHPNAEILEDQIVPLEAGAAYSAVFNSGMAAIQTAFFAYARPDST
CCCCCCCHHHHHHHCCCCCHHHHHCCCCEECCCCHHHHHHHCCHHHHHHHHHHEECCCCE
MVYTTPLYGGTTGLIHKFMKPLGVTGIPVTSGKTEAIEEAIRGARNCCMVYLETPANPTM
EEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEE
IMTDIERAAEAAAKHPDKPVVMVDNTFLGPSFQHPLLLGANICLYSATKYLSGYSDMLGG
EEHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHCCCHHHHHHHHHHCCHHHHHCC
VAITDSADIVQKLRSVRSMFGNILQPDECWMLDSRLSTVALRMNRQSKNAQRLAEALHGH
EEEECCHHHHHHHHHHHHHHCCCCCCCHHEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RLLSQVIYPTLFTDPEQKRIFEKQCDFPGGMFSIEFKGGKEAAFQFLRHVRIARNAVSLG
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCC
SVESLTCHPKSTTHSGMSAEELAESGVTDGLVRISVGIEDWRDLLADFEQALAAV
CCCEEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]