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The map label for this gene is citZ [H]

Identifier: 116623880

GI number: 116623880

Start: 6058547

End: 6059668

Strand: Direct

Name: citZ [H]

Synonym: Acid_4792

Alternate gene names: 116623880

Gene position: 6058547-6059668 (Clockwise)

Preceding gene: 116623879

Following gene: 116623881

Centisome position: 60.79

GC content: 62.83

Gene sequence:

>1122_bases
ATGGGAACGACTACGGCGGGGTTGGAAGGCATTATCGCGGGCGAGAGCGAGATCTGTTACATCGATGGCTACCTGGGAAT
CCTGAGCTATCGCGGATTCAACATCCACACGCTCGCCGACAACGCAAAATTCGAGGAAGTCATCTTCCTCCTCTGGAATG
GATGGCTGCCCCGGCAGGCCGAACTCGATGCCCTGAAAGCGGCGCTGGTCGCGGAGCGGGAACTGCCCGGCGCGATCGTG
GACTTCCTCAAGAGCGCGCCCAAGGTCAACACCATGGACCTGCTGCGCACCGCGGTATCCATGCTGAGCCTGCACGACGC
CGAGGCGCAGGATATGTCGGCCGAGGCGAACCATCGCAAGGCCATCCGCCTGATGTCGAAGACGGCGACGCTGGTCACCA
CCTATGACCGGCTCCGCAATGGGAAAGAGGTCATCCCCGGCGACCCGAAACTCAGCTTCGCGGCCAACTTCCTTTACACC
CTGACCGGCAATCGCCCCGACGAAATCATGGAGCGCGCCTTCGATGTGGCGATGACGCTGCATGCCGACCACGAGTTGAA
CGCCTCCACGTTCGCGGCGCGGGTGGTGGCGGCGACACTCTCCGACATCTACTCGGCGATCACGGCCGGCATCGGCGCAT
TGAAAGGCCCGCTGCACGGCGGCGCCAACCAGGACGTGATCAAGTGGCTGCTCAGCCTGGGTGACGAAGAGGAAGCCGTG
AAGGCCGTGGAGAATACGCTCGCCAGGAAGGTCAAGATTCCCGGCTTCGGCCACCGCGTCTACCGCACCGACGATCCGCG
CGCCACCCACCTCCGCGTCCTCAGCGAGGAACTGGGCAAGCGCACCGGCCACGAAAAGTTGTACCGCCTTTCCCAACGCA
TGGAAGCCACCGTGAAGGGCATCAAGGGGCTCAATCCCAACGTGGATTTCTACTCCGCGTCGGCCTACTATTCCCTGGGC
ATCCCGATCGATCTCTACACGCCGATCTTCGCCGTGAGCCGCATGTCGGGCTGGACCGCCCACGTGCTGGAGCAGTACCG
CAACAATCGTCTGATTCGTCCGCGCGCCGAATACAAGGGCAATCCGGACGGCCAGACCTGGATCCCGATCGACAAGCGGT
AA

Upstream 100 bases:

>100_bases
TCCGCCGGTTCAAGGCTGTCCGGCGGGCCTCTGAGGGTTGGATTGACATCATAATCTCGCGCGCGGGGGATGGCCCGGCG
CGTACAATTAGCGGAGCGCT

Downstream 100 bases:

>100_bases
CGACAAGCGGTAACCCCATGGATGAACGCGCCTATTACAGCGAATCGCAGACCACGAAGCCCGCGGTTCTGAATTGCCCC
TACTGCCGCAGCGCCGAGAC

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MGTTTAGLEGIIAGESEICYIDGYLGILSYRGFNIHTLADNAKFEEVIFLLWNGWLPRQAELDALKAALVAERELPGAIV
DFLKSAPKVNTMDLLRTAVSMLSLHDAEAQDMSAEANHRKAIRLMSKTATLVTTYDRLRNGKEVIPGDPKLSFAANFLYT
LTGNRPDEIMERAFDVAMTLHADHELNASTFAARVVAATLSDIYSAITAGIGALKGPLHGGANQDVIKWLLSLGDEEEAV
KAVENTLARKVKIPGFGHRVYRTDDPRATHLRVLSEELGKRTGHEKLYRLSQRMEATVKGIKGLNPNVDFYSASAYYSLG
IPIDLYTPIFAVSRMSGWTAHVLEQYRNNRLIRPRAEYKGNPDGQTWIPIDKR

Sequences:

>Translated_373_residues
MGTTTAGLEGIIAGESEICYIDGYLGILSYRGFNIHTLADNAKFEEVIFLLWNGWLPRQAELDALKAALVAERELPGAIV
DFLKSAPKVNTMDLLRTAVSMLSLHDAEAQDMSAEANHRKAIRLMSKTATLVTTYDRLRNGKEVIPGDPKLSFAANFLYT
LTGNRPDEIMERAFDVAMTLHADHELNASTFAARVVAATLSDIYSAITAGIGALKGPLHGGANQDVIKWLLSLGDEEEAV
KAVENTLARKVKIPGFGHRVYRTDDPRATHLRVLSEELGKRTGHEKLYRLSQRMEATVKGIKGLNPNVDFYSASAYYSLG
IPIDLYTPIFAVSRMSGWTAHVLEQYRNNRLIRPRAEYKGNPDGQTWIPIDKR
>Mature_372_residues
GTTTAGLEGIIAGESEICYIDGYLGILSYRGFNIHTLADNAKFEEVIFLLWNGWLPRQAELDALKAALVAERELPGAIVD
FLKSAPKVNTMDLLRTAVSMLSLHDAEAQDMSAEANHRKAIRLMSKTATLVTTYDRLRNGKEVIPGDPKLSFAANFLYTL
TGNRPDEIMERAFDVAMTLHADHELNASTFAARVVAATLSDIYSAITAGIGALKGPLHGGANQDVIKWLLSLGDEEEAVK
AVENTLARKVKIPGFGHRVYRTDDPRATHLRVLSEELGKRTGHEKLYRLSQRMEATVKGIKGLNPNVDFYSASAYYSLGI
PIDLYTPIFAVSRMSGWTAHVLEQYRNNRLIRPRAEYKGNPDGQTWIPIDKR

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=375, Percent_Identity=28.5333333333333, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1786527, Length=371, Percent_Identity=35.5795148247978, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1786939, Length=379, Percent_Identity=33.509234828496, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=373, Percent_Identity=27.8820375335121, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319850, Length=329, Percent_Identity=30.6990881458967, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324328, Length=322, Percent_Identity=30.7453416149068, Blast_Score=109, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6325257, Length=174, Percent_Identity=32.183908045977, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24640126, Length=378, Percent_Identity=29.6296296296296, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24640124, Length=378, Percent_Identity=29.6296296296296, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI21356863, Length=328, Percent_Identity=26.5243902439024, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 41304; Mature: 41172

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTTAGLEGIIAGESEICYIDGYLGILSYRGFNIHTLADNAKFEEVIFLLWNGWLPRQA
CCCCCCCCCEEEECCCCEEEECCHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCH
ELDALKAALVAERELPGAIVDFLKSAPKVNTMDLLRTAVSMLSLHDAEAQDMSAEANHRK
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCHHHHH
AIRLMSKTATLVTTYDRLRNGKEVIPGDPKLSFAANFLYTLTGNRPDEIMERAFDVAMTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHEE
HADHELNASTFAARVVAATLSDIYSAITAGIGALKGPLHGGANQDVIKWLLSLGDEEEAV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHH
KAVENTLARKVKIPGFGHRVYRTDDPRATHLRVLSEELGKRTGHEKLYRLSQRMEATVKG
HHHHHHHHHEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IKGLNPNVDFYSASAYYSLGIPIDLYTPIFAVSRMSGWTAHVLEQYRNNRLIRPRAEYKG
HCCCCCCCCEEECCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCHHHCCC
NPDGQTWIPIDKR
CCCCCEEECCCCC
>Mature Secondary Structure 
GTTTAGLEGIIAGESEICYIDGYLGILSYRGFNIHTLADNAKFEEVIFLLWNGWLPRQA
CCCCCCCCEEEECCCCEEEECCHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCCCCH
ELDALKAALVAERELPGAIVDFLKSAPKVNTMDLLRTAVSMLSLHDAEAQDMSAEANHRK
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCHHHHH
AIRLMSKTATLVTTYDRLRNGKEVIPGDPKLSFAANFLYTLTGNRPDEIMERAFDVAMTL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHEE
HADHELNASTFAARVVAATLSDIYSAITAGIGALKGPLHGGANQDVIKWLLSLGDEEEAV
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHH
KAVENTLARKVKIPGFGHRVYRTDDPRATHLRVLSEELGKRTGHEKLYRLSQRMEATVKG
HHHHHHHHHEECCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IKGLNPNVDFYSASAYYSLGIPIDLYTPIFAVSRMSGWTAHVLEQYRNNRLIRPRAEYKG
HCCCCCCCCEEECCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCHHHCCC
NPDGQTWIPIDKR
CCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]