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The map label for this gene is asnB [H]

Identifier: 116623712

GI number: 116623712

Start: 5847047

End: 5848942

Strand: Direct

Name: asnB [H]

Synonym: Acid_4624

Alternate gene names: 116623712

Gene position: 5847047-5848942 (Clockwise)

Preceding gene: 116623709

Following gene: 116623713

Centisome position: 58.67

GC content: 63.77

Gene sequence:

>1896_bases
ATGTGCGGCATCGCCGGTTACGCCCTCATCGATCAATCCGCCCAGCGCGCCCGCGTGCAGGCGATGTGCGACCAGATTCG
CCATCGCGGGCCGGATGACGAAGGCGTACACGTGGATCGCAATTGCGCGATTGGGATGCGGCGGCTGAGCATCATCGATC
TTTCCACAGGCCATCAGCCGATGTGCAACGAGGATGGGTCGGTGTGGATCGTCTTCAACGGCGAGATCTATGAGTATAAG
GAACTGCGCGCGGAACTGATCGCGCACGGGCATCAGTTTCGCACGGCGAGCGACACCGAGACACTGCTCCACCTGTACGA
AGAGGAAGGCGTGGAGGGATTGCGGCGGCTGCGGGGCATGTTCGCTTTCGCCATTTGGGATGCGCGGCGCAATCGCCTCA
TGCTGGCGCGCGATCCGTTCGGCAAGAAACCGCTTTACTATGCGGTGCTGCCTACGGGCATTTATTTCGGGAGTGAAATC
TCCTGCCTGCGCGAGGCGGGAGTGCCGCTGGAGCCGGATCCCGAAGCGCTGCGTCTCTATTTTCAGTTCAATTACATTCC
GGACCCGATGAGCGCGTATCGAGCGGTCCGACGGCTGCCCGCGGGAAGCTGGCTGACGTGGGAGAACGGGCAGGTGCGAC
AGGGGCGCTACTGGCAGCCGCCGGTGCCAGCCGCCCAACCCGTGGCCGGGCTCTCGCACGCGGACGCGGTGGTCAAGGTC
CGAGAGAAGTTCGATGAGGCTGTGCGAATTCGGATGATCGCCGACGTGCCGCTGGGCGCGTTTCTGAGCGGCGGGATCGA
TTCGAGCTCAATTGTGGCATCGATGGCGATGCAGTCGCGAGAGCCGATCAAGACCTTCTCCATTGGCTTCGAGGAGAGCG
AGTTCAACGAGCTGCCTTATGCGGCGATGGTCGCCAGGAAATATGGCGCCGACCATCATGAGATTGTGGTGCGGCCGGAT
TCGATCGGCATCATCCGCAAGTTCGCCAGGCATTTCGGCGAGCCGTTTGCGGATGCGTCGGCGATCCCGACCTATATCAT
GAGCGAGTTCGCGGCGCGCTACGTGAAGGTGGCGCTGAGCGGCGACGGAGGCGACGAGTTGTTCGGCGGCTACACCAGCC
TGCGCCAGGTGCAGAAGCTGGCGGCGTTCGATCGCGTGCCGCAGGCGGTGAGGCTGATGATGTCGTGGGCGTCCTCGCGG
ATGCCTTACTCGGCGTACGGCAAGAATTTTCTCCACATGCTGGGGCGGCGGAGCGCGCTGGACCGCTACTTCGCGTTCAA
CTACGCGCCGTTCCACATGCGCTCCAAGCTGCTGCGGCCGGATTGGATGCTGCCCGCCGAAAGCGGATTCCTGGCGCGCG
CGCTACCGGATTGCATTCTGCCGGAGGACGCCGGGGCGCTCTCACAGGTGATGTACTTTGAGGGCACGGCCAACCTGACG
GGCGACATGCTGGTGAAGGTGGATCGCATGTCGATGGCGGCGTCGCTCGAAGTGCGCTGCCCGCTGCTCGATCACGAGCT
CGCGGAGTTGGCGGCGACGATTCCGCACGCGTGGAAGGTCGTGGGCGGCCAGGGGAAGCGCATATTGCTGGACGCTCTGG
GGGACCGGCTGCCGCCCGAGTTGCTCAATCGTCCCAAGACGGGATTTGGAGTGCCGCTGGTGGATTGGTTCCGAACGTCG
TTGCGCGAATTGCTGTGGGACACGCTGACGGCGGAGAGATTTCTGGCGCACAACATCGTGGCGCCGGAGTTCCTGCGGAC
GCTGCTCGAGGAGCACGATAGCGGACGGCGGGACAACAGCCAATGGCTGTTCACGCTGCTGGTGCTGGCGCTTTGGTTCG
AGGATATCGAACAGCCTCTGGCCTCGCCGGTGCCGGCGAGGGTGGAAGGGGCGTAG

Upstream 100 bases:

>100_bases
GCGAGTGGTTGCAGCCATTCTCACGAGTGTAATACGAAGTACCCCGGCTGCGACAACTTCAGCCGTTTGCCGCCGGGATG
GTAAATTCGAAAGAGTACCT

Downstream 100 bases:

>100_bases
GGCGAGCTCGTGTCCGCGGAAGCCGTCTGAAAGGCGGCTGCAGCCACGATTGGCTGCCCCACAAAGGGGTTTTCATCCCC
TTAAATGGCGCCGAGCGCCA

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MCGIAGYALIDQSAQRARVQAMCDQIRHRGPDDEGVHVDRNCAIGMRRLSIIDLSTGHQPMCNEDGSVWIVFNGEIYEYK
ELRAELIAHGHQFRTASDTETLLHLYEEEGVEGLRRLRGMFAFAIWDARRNRLMLARDPFGKKPLYYAVLPTGIYFGSEI
SCLREAGVPLEPDPEALRLYFQFNYIPDPMSAYRAVRRLPAGSWLTWENGQVRQGRYWQPPVPAAQPVAGLSHADAVVKV
REKFDEAVRIRMIADVPLGAFLSGGIDSSSIVASMAMQSREPIKTFSIGFEESEFNELPYAAMVARKYGADHHEIVVRPD
SIGIIRKFARHFGEPFADASAIPTYIMSEFAARYVKVALSGDGGDELFGGYTSLRQVQKLAAFDRVPQAVRLMMSWASSR
MPYSAYGKNFLHMLGRRSALDRYFAFNYAPFHMRSKLLRPDWMLPAESGFLARALPDCILPEDAGALSQVMYFEGTANLT
GDMLVKVDRMSMAASLEVRCPLLDHELAELAATIPHAWKVVGGQGKRILLDALGDRLPPELLNRPKTGFGVPLVDWFRTS
LRELLWDTLTAERFLAHNIVAPEFLRTLLEEHDSGRRDNSQWLFTLLVLALWFEDIEQPLASPVPARVEGA

Sequences:

>Translated_631_residues
MCGIAGYALIDQSAQRARVQAMCDQIRHRGPDDEGVHVDRNCAIGMRRLSIIDLSTGHQPMCNEDGSVWIVFNGEIYEYK
ELRAELIAHGHQFRTASDTETLLHLYEEEGVEGLRRLRGMFAFAIWDARRNRLMLARDPFGKKPLYYAVLPTGIYFGSEI
SCLREAGVPLEPDPEALRLYFQFNYIPDPMSAYRAVRRLPAGSWLTWENGQVRQGRYWQPPVPAAQPVAGLSHADAVVKV
REKFDEAVRIRMIADVPLGAFLSGGIDSSSIVASMAMQSREPIKTFSIGFEESEFNELPYAAMVARKYGADHHEIVVRPD
SIGIIRKFARHFGEPFADASAIPTYIMSEFAARYVKVALSGDGGDELFGGYTSLRQVQKLAAFDRVPQAVRLMMSWASSR
MPYSAYGKNFLHMLGRRSALDRYFAFNYAPFHMRSKLLRPDWMLPAESGFLARALPDCILPEDAGALSQVMYFEGTANLT
GDMLVKVDRMSMAASLEVRCPLLDHELAELAATIPHAWKVVGGQGKRILLDALGDRLPPELLNRPKTGFGVPLVDWFRTS
LRELLWDTLTAERFLAHNIVAPEFLRTLLEEHDSGRRDNSQWLFTLLVLALWFEDIEQPLASPVPARVEGA
>Mature_631_residues
MCGIAGYALIDQSAQRARVQAMCDQIRHRGPDDEGVHVDRNCAIGMRRLSIIDLSTGHQPMCNEDGSVWIVFNGEIYEYK
ELRAELIAHGHQFRTASDTETLLHLYEEEGVEGLRRLRGMFAFAIWDARRNRLMLARDPFGKKPLYYAVLPTGIYFGSEI
SCLREAGVPLEPDPEALRLYFQFNYIPDPMSAYRAVRRLPAGSWLTWENGQVRQGRYWQPPVPAAQPVAGLSHADAVVKV
REKFDEAVRIRMIADVPLGAFLSGGIDSSSIVASMAMQSREPIKTFSIGFEESEFNELPYAAMVARKYGADHHEIVVRPD
SIGIIRKFARHFGEPFADASAIPTYIMSEFAARYVKVALSGDGGDELFGGYTSLRQVQKLAAFDRVPQAVRLMMSWASSR
MPYSAYGKNFLHMLGRRSALDRYFAFNYAPFHMRSKLLRPDWMLPAESGFLARALPDCILPEDAGALSQVMYFEGTANLT
GDMLVKVDRMSMAASLEVRCPLLDHELAELAATIPHAWKVVGGQGKRILLDALGDRLPPELLNRPKTGFGVPLVDWFRTS
LRELLWDTLTAERFLAHNIVAPEFLRTLLEEHDSGRRDNSQWLFTLLVLALWFEDIEQPLASPVPARVEGA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=402, Percent_Identity=28.3582089552239, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI168229252, Length=402, Percent_Identity=28.3582089552239, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI168229250, Length=402, Percent_Identity=28.3582089552239, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI296010848, Length=378, Percent_Identity=28.3068783068783, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1786889, Length=461, Percent_Identity=30.8026030368764, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI71993933, Length=390, Percent_Identity=28.2051282051282, Blast_Score=122, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=389, Percent_Identity=28.2776349614396, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25147560, Length=346, Percent_Identity=27.1676300578035, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=468, Percent_Identity=28.6324786324786, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325403, Length=431, Percent_Identity=27.8422273781903, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553209, Length=360, Percent_Identity=33.8888888888889, Blast_Score=165, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70947; Mature: 70947

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGYALIDQSAQRARVQAMCDQIRHRGPDDEGVHVDRNCAIGMRRLSIIDLSTGHQP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHCCEEEEEEEECCCCCC
MCNEDGSVWIVFNGEIYEYKELRAELIAHGHQFRTASDTETLLHLYEEEGVEGLRRLRGM
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FAFAIWDARRNRLMLARDPFGKKPLYYAVLPTGIYFGSEISCLREAGVPLEPDPEALRLY
HHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHCCCHHHHHHHCCCCCCCCHHHEEEE
FQFNYIPDPMSAYRAVRRLPAGSWLTWENGQVRQGRYWQPPVPAAQPVAGLSHADAVVKV
EEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
REKFDEAVRIRMIADVPLGAFLSGGIDSSSIVASMAMQSREPIKTFSIGFEESEFNELPY
HHHHHHHHEEEEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEECCCCCCHHCCCCH
AAMVARKYGADHHEIVVRPDSIGIIRKFARHFGEPFADASAIPTYIMSEFAARYVKVALS
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEC
GDGGDELFGGYTSLRQVQKLAAFDRVPQAVRLMMSWASSRMPYSAYGKNFLHMLGRRSAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
DRYFAFNYAPFHMRSKLLRPDWMLPAESGFLARALPDCILPEDAGALSQVMYFEGTANLT
HHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHEECCCCCCC
GDMLVKVDRMSMAASLEVRCPLLDHELAELAATIPHAWKVVGGQGKRILLDALGDRLPPE
CCCEEEEHHHHHHHCCEEECCHHHHHHHHHHHHCCHHHEEECCCCCEEHHHHHCCCCCHH
LLNRPKTGFGVPLVDWFRTSLRELLWDTLTAERFLAHNIVAPEFLRTLLEEHDSGRRDNS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCH
QWLFTLLVLALWFEDIEQPLASPVPARVEGA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MCGIAGYALIDQSAQRARVQAMCDQIRHRGPDDEGVHVDRNCAIGMRRLSIIDLSTGHQP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHCCEEEEEEEECCCCCC
MCNEDGSVWIVFNGEIYEYKELRAELIAHGHQFRTASDTETLLHLYEEEGVEGLRRLRGM
CCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FAFAIWDARRNRLMLARDPFGKKPLYYAVLPTGIYFGSEISCLREAGVPLEPDPEALRLY
HHHHHHHHCCCEEEEEECCCCCCCEEEEEECCCHHCCCHHHHHHHCCCCCCCCHHHEEEE
FQFNYIPDPMSAYRAVRRLPAGSWLTWENGQVRQGRYWQPPVPAAQPVAGLSHADAVVKV
EEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
REKFDEAVRIRMIADVPLGAFLSGGIDSSSIVASMAMQSREPIKTFSIGFEESEFNELPY
HHHHHHHHEEEEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCEECCCCCCHHCCCCH
AAMVARKYGADHHEIVVRPDSIGIIRKFARHFGEPFADASAIPTYIMSEFAARYVKVALS
HHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHEEEEEC
GDGGDELFGGYTSLRQVQKLAAFDRVPQAVRLMMSWASSRMPYSAYGKNFLHMLGRRSAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
DRYFAFNYAPFHMRSKLLRPDWMLPAESGFLARALPDCILPEDAGALSQVMYFEGTANLT
HHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHEECCCCCCC
GDMLVKVDRMSMAASLEVRCPLLDHELAELAATIPHAWKVVGGQGKRILLDALGDRLPPE
CCCEEEEHHHHHHHCCEEECCHHHHHHHHHHHHCCHHHEEECCCCCEEHHHHHCCCCCHH
LLNRPKTGFGVPLVDWFRTSLRELLWDTLTAERFLAHNIVAPEFLRTLLEEHDSGRRDNS
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCH
QWLFTLLVLALWFEDIEQPLASPVPARVEGA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]