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The map label for this gene is pleD [H]

Identifier: 116623704

GI number: 116623704

Start: 5834889

End: 5835866

Strand: Direct

Name: pleD [H]

Synonym: Acid_4616

Alternate gene names: 116623704

Gene position: 5834889-5835866 (Clockwise)

Preceding gene: 116623703

Following gene: 116623707

Centisome position: 58.55

GC content: 63.09

Gene sequence:

>978_bases
TTGCCGAACGCATCCAGCAATACCGCCGGACTGCTCGCCCTTTGCAACACGCATCGGCCCATGCGTGTGCTAGCGGCGGA
AGACAACCCGGTTTTTCAATCGATGTTGAAGACCATGTTGACGCGGTGGGGCTATGAGGCGGTGATCGCGCGCGACGGCA
CCGAGGCCTGGAACATTCTGGAAGGCGATGGCGCACCCAGGCTGGCGATCTTGGATTGGATGATGCCTGGAATGGATGGC
GTAGAGATCTGCCGCCGGATTCGCAGCGCCAACCGGGAACCTTATGTGTATATTCTTCTGCTGACGGCACGCACGGAATC
GCAGGACTTGATCGAAGGGATGGACGCGGGCGCGGACGATTATCTGACCAAGCCGTTCAATGCGCATGAATTGCGCGTCC
GGATCCGAGCCGGCCGGCGGATTCTGGACCTGCAGGAGGAATTGCTAAAGGCCCGCGAGGCGCTGCGCGAGCAGGCGACG
CATGACGGGCTGACGGGCCTTCTCAATCGCACGAGCATTCTAGAGAAGCTGGATGACGAACTTTCGCGCGCCGCCCGCGA
CGGCAGTTCCGTCTCGGTGCTGATGGTGGATCTGGACCGCTTCAAATCGGTCAACGACACGCAGGGACATCTGGCGGGGG
ACGCGGTGCTGCGCGAGGCTTCGAGCCGCCTGAGATCGGCATCGCGAAGGTACGATTCGGTGGGCCGCTATGGCGGCGAG
GAATTTCTAGTGGTGCTTCCCGGCTGCGAAAAGGCAGCGGCGGAGTTGCAGGCCGAGCGGCTGCGCGAGGCGATCGGCGG
GACTCCGTTCCGGGCGGATTCACGTCCGCTGGCGATGACGTGCAGCATTGGGCTGGCATGCTCCAGCCACTGCGCGCCTG
AGGATTTAATCCGCGAGGCGGACGACGCGCTTTACCAGGCGAAGGCGGGCGGGCGCAACCGGGTGGTGGCGCACACGGAG
CTTTCGATGGGTATTTAG

Upstream 100 bases:

>100_bases
GGACTGGTCCGGACGGTTGCCGGAACCGAAATAAGCCTTAGAACCACGGGGCCATCAAATTTCGCGGTTTGCAGCCGATG
AGTGACACAGGGAAACAAGA

Downstream 100 bases:

>100_bases
CTGTTGATATCTTCGTGAGCTTCGATGCGTTTCTTGAGGCCATCGAGTTGGGACTTCTGCGGTTCTGGGGCTGCCGACTG
GGCGGCCTGGGTTTCCTTCA

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MPNASSNTAGLLALCNTHRPMRVLAAEDNPVFQSMLKTMLTRWGYEAVIARDGTEAWNILEGDGAPRLAILDWMMPGMDG
VEICRRIRSANREPYVYILLLTARTESQDLIEGMDAGADDYLTKPFNAHELRVRIRAGRRILDLQEELLKAREALREQAT
HDGLTGLLNRTSILEKLDDELSRAARDGSSVSVLMVDLDRFKSVNDTQGHLAGDAVLREASSRLRSASRRYDSVGRYGGE
EFLVVLPGCEKAAAELQAERLREAIGGTPFRADSRPLAMTCSIGLACSSHCAPEDLIREADDALYQAKAGGRNRVVAHTE
LSMGI

Sequences:

>Translated_325_residues
MPNASSNTAGLLALCNTHRPMRVLAAEDNPVFQSMLKTMLTRWGYEAVIARDGTEAWNILEGDGAPRLAILDWMMPGMDG
VEICRRIRSANREPYVYILLLTARTESQDLIEGMDAGADDYLTKPFNAHELRVRIRAGRRILDLQEELLKAREALREQAT
HDGLTGLLNRTSILEKLDDELSRAARDGSSVSVLMVDLDRFKSVNDTQGHLAGDAVLREASSRLRSASRRYDSVGRYGGE
EFLVVLPGCEKAAAELQAERLREAIGGTPFRADSRPLAMTCSIGLACSSHCAPEDLIREADDALYQAKAGGRNRVVAHTE
LSMGI
>Mature_324_residues
PNASSNTAGLLALCNTHRPMRVLAAEDNPVFQSMLKTMLTRWGYEAVIARDGTEAWNILEGDGAPRLAILDWMMPGMDGV
EICRRIRSANREPYVYILLLTARTESQDLIEGMDAGADDYLTKPFNAHELRVRIRAGRRILDLQEELLKAREALREQATH
DGLTGLLNRTSILEKLDDELSRAARDGSSVSVLMVDLDRFKSVNDTQGHLAGDAVLREASSRLRSASRRYDSVGRYGGEE
FLVVLPGCEKAAAELQAERLREAIGGTPFRADSRPLAMTCSIGLACSSHCAPEDLIREADDALYQAKAGGRNRVVAHTEL
SMGI

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=200, Percent_Identity=39, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=36.3128491620112, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787541, Length=179, Percent_Identity=37.4301675977654, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=40, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=37.9518072289157, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787262, Length=177, Percent_Identity=35.0282485875706, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=33.5260115606936, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787816, Length=180, Percent_Identity=33.8888888888889, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=172, Percent_Identity=34.3023255813954, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786599, Length=119, Percent_Identity=36.9747899159664, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87081977, Length=184, Percent_Identity=34.2391304347826, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=38.4615384615385, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87082012, Length=121, Percent_Identity=40.495867768595, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787375, Length=120, Percent_Identity=40.8333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=34.3373493975904, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=36, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790863, Length=114, Percent_Identity=37.719298245614, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788394, Length=127, Percent_Identity=33.0708661417323, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI2367329, Length=116, Percent_Identity=35.3448275862069, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=27.2151898734177, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=38.4615384615385, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787229, Length=117, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=30.2521008403361, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788550, Length=134, Percent_Identity=29.1044776119403, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786911, Length=119, Percent_Identity=38.655462184874, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789402, Length=121, Percent_Identity=38.8429752066116, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=169, Percent_Identity=31.9526627218935, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790552, Length=117, Percent_Identity=37.6068376068376, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 35769; Mature: 35638

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNASSNTAGLLALCNTHRPMRVLAAEDNPVFQSMLKTMLTRWGYEAVIARDGTEAWNIL
CCCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEE
EGDGAPRLAILDWMMPGMDGVEICRRIRSANREPYVYILLLTARTESQDLIEGMDAGADD
ECCCCCCEEHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHCCCCCCH
YLTKPFNAHELRVRIRAGRRILDLQEELLKAREALREQATHDGLTGLLNRTSILEKLDDE
HHCCCCCCHHEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LSRAARDGSSVSVLMVDLDRFKSVNDTQGHLAGDAVLREASSRLRSASRRYDSVGRYGGE
HHHHHCCCCCEEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EFLVVLPGCEKAAAELQAERLREAIGGTPFRADSRPLAMTCSIGLACSSHCAPEDLIREA
EEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHCCCCCHHHHHHHH
DDALYQAKAGGRNRVVAHTELSMGI
HHHHHHHCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
PNASSNTAGLLALCNTHRPMRVLAAEDNPVFQSMLKTMLTRWGYEAVIARDGTEAWNIL
CCCCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEE
EGDGAPRLAILDWMMPGMDGVEICRRIRSANREPYVYILLLTARTESQDLIEGMDAGADD
ECCCCCCEEHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHCCCCCCH
YLTKPFNAHELRVRIRAGRRILDLQEELLKAREALREQATHDGLTGLLNRTSILEKLDDE
HHCCCCCCHHEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
LSRAARDGSSVSVLMVDLDRFKSVNDTQGHLAGDAVLREASSRLRSASRRYDSVGRYGGE
HHHHHCCCCCEEEEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EFLVVLPGCEKAAAELQAERLREAIGGTPFRADSRPLAMTCSIGLACSSHCAPEDLIREA
EEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCHHCCCCCHHHHHHHH
DDALYQAKAGGRNRVVAHTELSMGI
HHHHHHHCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA