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The map label for this gene is miaE [H]

Identifier: 116623680

GI number: 116623680

Start: 5807680

End: 5808345

Strand: Reverse

Name: miaE [H]

Synonym: Acid_4592

Alternate gene names: 116623680

Gene position: 5808345-5807680 (Counterclockwise)

Preceding gene: 116623682

Following gene: 116623679

Centisome position: 58.28

GC content: 66.07

Gene sequence:

>666_bases
TTGACCTCGCGGCACAAGTGGCCGCTTTGCTACCGTGAAACAGTGAACCCGACTGAACTCGCGCAGGTGGACGCCTTGCC
GCTGCATTCGCGCACGCCGAAAGAGTGGGCCCGCGCGGTACTGGCGGAGCCGATCCGCCTGCTGATCGACCACGCGTTCC
TGGAAAAGAAGGCCGCGGCCAACGCGATGGAACTGCTCACACGTTGGCCGAACGATGGCGTGGAAGGCTGGGTCGAGACC
ATGACCTCCGTGGCCCGGGACGAAACGGCACACTTGGCGCAGGTGACGCGCATTCTGCTGAGCCGGGGCGGCAGGCTGGA
GCGCGTCCACAAGAACCCCTACGCGAATTCGCTGCGGCTCCTGGTGCGGAAGGGAGACCCGGCGGAGATACTGGACCGGC
TGCTGGTCTCGGCGCTAATTGAGGTCCGCTCCTGCGAACGGTTTGCGCTGCTGGCGGCGGAGGCGGAGGATGCCGAACTG
GGCGCATTCTATCGCGGGCTCTTCCTTTCCGAGTTCGGGCATTACAAGGTATTCTTGGCGCTGGCGCGGAAGATCGCCAA
GCCCGCCGCGATGGAAGCGCGGTGGCAGGAGATGCTGGCGGCGGAGGCGCGCATCCTGGCGGAACAGGCCGCCGGACCGC
GCATCCACTCGGGGCATCTTACCTGA

Upstream 100 bases:

>100_bases
GACGCGGTCCAGATAGGTGACGGCGGCGAGCAGCATCAACAGTCCGGCTGCGCGGTATCGGGTTCGCGTGGGAAGCATCG
CCCCAACAGCATCTCTGTTT

Downstream 100 bases:

>100_bases
ACTTACCGCGTCTTACTTCAGCGGCAACGCCGGTCGTGATAGGGTCGGTCTTATGAATCCTTATGCATCGTTCCTTGGAG
ACCGCGATCCCTTGAGCGTG

Product: tRNA--hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTSRHKWPLCYRETVNPTELAQVDALPLHSRTPKEWARAVLAEPIRLLIDHAFLEKKAAANAMELLTRWPNDGVEGWVET
MTSVARDETAHLAQVTRILLSRGGRLERVHKNPYANSLRLLVRKGDPAEILDRLLVSALIEVRSCERFALLAAEAEDAEL
GAFYRGLFLSEFGHYKVFLALARKIAKPAAMEARWQEMLAAEARILAEQAAGPRIHSGHLT

Sequences:

>Translated_221_residues
MTSRHKWPLCYRETVNPTELAQVDALPLHSRTPKEWARAVLAEPIRLLIDHAFLEKKAAANAMELLTRWPNDGVEGWVET
MTSVARDETAHLAQVTRILLSRGGRLERVHKNPYANSLRLLVRKGDPAEILDRLLVSALIEVRSCERFALLAAEAEDAEL
GAFYRGLFLSEFGHYKVFLALARKIAKPAAMEARWQEMLAAEARILAEQAAGPRIHSGHLT
>Mature_220_residues
TSRHKWPLCYRETVNPTELAQVDALPLHSRTPKEWARAVLAEPIRLLIDHAFLEKKAAANAMELLTRWPNDGVEGWVETM
TSVARDETAHLAQVTRILLSRGGRLERVHKNPYANSLRLLVRKGDPAEILDRLLVSALIEVRSCERFALLAAEAEDAELG
AFYRGLFLSEFGHYKVFLALARKIAKPAAMEARWQEMLAAEARILAEQAAGPRIHSGHLT

Specific function: Production of the modified nucleoside 2-methylthio-cis- ribozeatin (MS[2]IO[6]A) found in some tRNAs. Catalyzes the oxygen-dependent transformation of MS[2]I[6]A into MS[2]IO[6]A [H]

COG id: COG4445

COG function: function code FJ; Hydroxylase for synthesis of 2-methylthio-cis-ribozeatin in tRNA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012347
- InterPro:   IPR010386 [H]

Pfam domain/function: PF06175 MiaE [H]

EC number: NA

Molecular weight: Translated: 24834; Mature: 24703

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRHKWPLCYRETVNPTELAQVDALPLHSRTPKEWARAVLAEPIRLLIDHAFLEKKAAA
CCCCCCCCCHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAMELLTRWPNDGVEGWVETMTSVARDETAHLAQVTRILLSRGGRLERVHKNPYANSLRL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHH
LVRKGDPAEILDRLLVSALIEVRSCERFALLAAEAEDAELGAFYRGLFLSEFGHYKVFLA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHH
LARKIAKPAAMEARWQEMLAAEARILAEQAAGPRIHSGHLT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TSRHKWPLCYRETVNPTELAQVDALPLHSRTPKEWARAVLAEPIRLLIDHAFLEKKAAA
CCCCCCCCHHHCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NAMELLTRWPNDGVEGWVETMTSVARDETAHLAQVTRILLSRGGRLERVHKNPYANSLRL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHH
LVRKGDPAEILDRLLVSALIEVRSCERFALLAAEAEDAELGAFYRGLFLSEFGHYKVFLA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHH
LARKIAKPAAMEARWQEMLAAEARILAEQAAGPRIHSGHLT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253666; 11677609 [H]