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The map label for this gene is rnjA [H]

Identifier: 116623576

GI number: 116623576

Start: 5679663

End: 5681333

Strand: Reverse

Name: rnjA [H]

Synonym: Acid_4486

Alternate gene names: 116623576

Gene position: 5681333-5679663 (Counterclockwise)

Preceding gene: 116623577

Following gene: 116623575

Centisome position: 57.01

GC content: 61.7

Gene sequence:

>1671_bases
ATGACTGATTTGAAGTTACAGGTAATCCCTCTCGGCGGTCTGGGCGAGTTCGGTATGAACTCCACCGCCATCCGCTACGG
CGAAGACATTATCGTGGTCGATTGCGGAATGATGTTCCCCGACGCCGAGCTCCTGGGTGTCGATCTGGTCATGCCCGACC
TGACGTTCCTGAAGGAGAACCAGTCCCAGGTGCGCGCGCTGATTCTCACGCACGGACATGAGGACCACATCGGGGCGGTG
CCCTACATGCTCTCCGAGATCGACGTGCCTGTTTATGGCACGCCGTTCACGCTGTCTCTGGTTGAGCGGCGCATGGAAGA
GTACGACCTGGAGAAGGAGCCGCGGCTGATCAAGGTCAAGCCGAAGCAGGCGATTGCGGTAGGGCCCTTCAAGATCGAGT
TTATTCACGTTACGCACTCGATTGTGAGCGCGGTGGCGCTGGCGATCACGACGCCGCTCGGCGTGATCATCCACACGGGC
GATTTCAAGGTGGATCCCACCCCCACCGATAATGAGCTTTTCGACCTGCACACGCTGGCCGATTATGGCAAGCGCGGGGT
CCTGCTGCTGCTCTCCGATTCCACCAACGCCGACCGCCCCGGATATACGGAGAGCGAGCGCGCGGTGAAGCCGCGTATGG
AAGAGATCTTCAGCCGCGCGGAGAAGCGGATTGTGGTGTCCTGCTTCAGCAGCTCGATCCACCGCATCCAGCTGGTGCTC
GACCTGGCGGAGGAATACGGACGGCGGGTCGCGGTAATCGGCCGAAGCATGACCTCGGTGACCGAGATCGCGCACTCGCA
CGGACTGCTCAGTATTCCAGACGGCATCCTGCTGCGTCCCCAGGACGCGATGGACCTGCCGGCAGGGAAGGTCGCGTTCC
TGGTTTCGGGGACGCAGGGCGAGCCCATGTCGGCGCTCTCGCGGGTGGCGGTGGACAATCACAAGCACGTCTCGGTGGAG
CGCGGCGATACGGTTGTGCTGAGCTCGCGAATCATCCCGGGCAACGAGAAGGGCATCTTCCGGATGATCGACCACATGGC
GCGCCGCGGCGCGGACGTCATGTACGGCAGCATGAACCCCCCGCTGCACGTCTCCGGCCACGGCAGCATCGAGGAAATGA
AGCTGGTGTTGAACCTGGTGCGGCCGCGCTTCTTCATGCCGATCCACGGCGAGTTCCGCCAGCTTTCCAAGCACGCCCGG
CTGGCGGAGCACCTGCGGTTTGCCGGCCTGGAAGAGACCTTCGTGATGGACACCGGCGATATTCTGGAGTTCGACCACCA
CGGCGCGCGCAAGGCCGGGCGGGTTACGGTGGGTCACGTCTGCATCGATTCCGGTTCGGTGGACGACGTTGTTCAGGAAA
TGGTGATTCGCGACCGGCGCCTGCTTTCCGAAGACGGCATCGTGCTCGCGATCATCGCTATTAATCGTCACACGGGCAAA
CTGGAAAGTCCGCCGGAGATTGTGAGCCGGGGTTTCAATGTCGGCATTGAAGGCAGCGCTTTCATGGAGACCGCGAAGGC
GATCGTGGCCAAGACCCTGGAGAGTTCGAGCCAGGAGGAAAAGACCGATTGGGGCGTGATGAAGGAGAAGGTCCGCGCGG
ACCTGAAACGGTACATCGTGAAGGAGACTTCGCGGCGGCCGCTGATTCTTCCCGTGATCATGGAGGTATAA

Upstream 100 bases:

>100_bases
AACAAGGTACCCATAAGCAACTCCTCGAGCTCGGCGGGCGTTATGCTGAATTATTCGAACTGCAGGCTGCGGGGTATCGC
TGAAAGCAAAGTGAAGACGA

Downstream 100 bases:

>100_bases
GAGCGGTGGTTCATGGGGGACGCTCCCTGACGGTCGCGGCTCTGATTGGGGCGGCGAGTTGATTGGGGCGGCGAGTTGGT
TGGGGCGGCGAGTTGATTGG

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MTDLKLQVIPLGGLGEFGMNSTAIRYGEDIIVVDCGMMFPDAELLGVDLVMPDLTFLKENQSQVRALILTHGHEDHIGAV
PYMLSEIDVPVYGTPFTLSLVERRMEEYDLEKEPRLIKVKPKQAIAVGPFKIEFIHVTHSIVSAVALAITTPLGVIIHTG
DFKVDPTPTDNELFDLHTLADYGKRGVLLLLSDSTNADRPGYTESERAVKPRMEEIFSRAEKRIVVSCFSSSIHRIQLVL
DLAEEYGRRVAVIGRSMTSVTEIAHSHGLLSIPDGILLRPQDAMDLPAGKVAFLVSGTQGEPMSALSRVAVDNHKHVSVE
RGDTVVLSSRIIPGNEKGIFRMIDHMARRGADVMYGSMNPPLHVSGHGSIEEMKLVLNLVRPRFFMPIHGEFRQLSKHAR
LAEHLRFAGLEETFVMDTGDILEFDHHGARKAGRVTVGHVCIDSGSVDDVVQEMVIRDRRLLSEDGIVLAIIAINRHTGK
LESPPEIVSRGFNVGIEGSAFMETAKAIVAKTLESSSQEEKTDWGVMKEKVRADLKRYIVKETSRRPLILPVIMEV

Sequences:

>Translated_556_residues
MTDLKLQVIPLGGLGEFGMNSTAIRYGEDIIVVDCGMMFPDAELLGVDLVMPDLTFLKENQSQVRALILTHGHEDHIGAV
PYMLSEIDVPVYGTPFTLSLVERRMEEYDLEKEPRLIKVKPKQAIAVGPFKIEFIHVTHSIVSAVALAITTPLGVIIHTG
DFKVDPTPTDNELFDLHTLADYGKRGVLLLLSDSTNADRPGYTESERAVKPRMEEIFSRAEKRIVVSCFSSSIHRIQLVL
DLAEEYGRRVAVIGRSMTSVTEIAHSHGLLSIPDGILLRPQDAMDLPAGKVAFLVSGTQGEPMSALSRVAVDNHKHVSVE
RGDTVVLSSRIIPGNEKGIFRMIDHMARRGADVMYGSMNPPLHVSGHGSIEEMKLVLNLVRPRFFMPIHGEFRQLSKHAR
LAEHLRFAGLEETFVMDTGDILEFDHHGARKAGRVTVGHVCIDSGSVDDVVQEMVIRDRRLLSEDGIVLAIIAINRHTGK
LESPPEIVSRGFNVGIEGSAFMETAKAIVAKTLESSSQEEKTDWGVMKEKVRADLKRYIVKETSRRPLILPVIMEV
>Mature_555_residues
TDLKLQVIPLGGLGEFGMNSTAIRYGEDIIVVDCGMMFPDAELLGVDLVMPDLTFLKENQSQVRALILTHGHEDHIGAVP
YMLSEIDVPVYGTPFTLSLVERRMEEYDLEKEPRLIKVKPKQAIAVGPFKIEFIHVTHSIVSAVALAITTPLGVIIHTGD
FKVDPTPTDNELFDLHTLADYGKRGVLLLLSDSTNADRPGYTESERAVKPRMEEIFSRAEKRIVVSCFSSSIHRIQLVLD
LAEEYGRRVAVIGRSMTSVTEIAHSHGLLSIPDGILLRPQDAMDLPAGKVAFLVSGTQGEPMSALSRVAVDNHKHVSVER
GDTVVLSSRIIPGNEKGIFRMIDHMARRGADVMYGSMNPPLHVSGHGSIEEMKLVLNLVRPRFFMPIHGEFRQLSKHARL
AEHLRFAGLEETFVMDTGDILEFDHHGARKAGRVTVGHVCIDSGSVDDVVQEMVIRDRRLLSEDGIVLAIIAINRHTGKL
ESPPEIVSRGFNVGIEGSAFMETAKAIVAKTLESSSQEEKTDWGVMKEKVRADLKRYIVKETSRRPLILPVIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61562; Mature: 61431

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLKLQVIPLGGLGEFGMNSTAIRYGEDIIVVDCGMMFPDAELLGVDLVMPDLTFLKEN
CCCCEEEEEEECCCCCCCCCCCEEEECCEEEEEECCCCCCCHHHEEEEEECCCHHHHHCC
QSQVRALILTHGHEDHIGAVPYMLSEIDVPVYGTPFTLSLVERRMEEYDLEKEPRLIKVK
HHHEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEC
PKQAIAVGPFKIEFIHVTHSIVSAVALAITTPLGVIIHTGDFKVDPTPTDNELFDLHTLA
CCCEEEECCEEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCEEEHHHHH
DYGKRGVLLLLSDSTNADRPGYTESERAVKPRMEEIFSRAEKRIVVSCFSSSIHRIQLVL
HCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEHHHHCCHHHHHHHH
DLAEEYGRRVAVIGRSMTSVTEIAHSHGLLSIPDGILLRPQDAMDLPAGKVAFLVSGTQG
HHHHHHCCEEEEECCHHHHHHHHHHHCCEEECCCCEEECCCCCCCCCCCCEEEEEECCCC
EPMSALSRVAVDNHKHVSVERGDTVVLSSRIIPGNEKGIFRMIDHMARRGADVMYGSMNP
CHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCC
PLHVSGHGSIEEMKLVLNLVRPRFFMPIHGEFRQLSKHARLAEHLRFAGLEETFVMDTGD
CEEECCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
ILEFDHHGARKAGRVTVGHVCIDSGSVDDVVQEMVIRDRRLLSEDGIVLAIIAINRHTGK
EEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCC
LESPPEIVSRGFNVGIEGSAFMETAKAIVAKTLESSSQEEKTDWGVMKEKVRADLKRYIV
CCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KETSRRPLILPVIMEV
HHCCCCCEEEEEEECC
>Mature Secondary Structure 
TDLKLQVIPLGGLGEFGMNSTAIRYGEDIIVVDCGMMFPDAELLGVDLVMPDLTFLKEN
CCCEEEEEEECCCCCCCCCCCEEEECCEEEEEECCCCCCCHHHEEEEEECCCHHHHHCC
QSQVRALILTHGHEDHIGAVPYMLSEIDVPVYGTPFTLSLVERRMEEYDLEKEPRLIKVK
HHHEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEC
PKQAIAVGPFKIEFIHVTHSIVSAVALAITTPLGVIIHTGDFKVDPTPTDNELFDLHTLA
CCCEEEECCEEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCEEEHHHHH
DYGKRGVLLLLSDSTNADRPGYTESERAVKPRMEEIFSRAEKRIVVSCFSSSIHRIQLVL
HCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEHHHHCCHHHHHHHH
DLAEEYGRRVAVIGRSMTSVTEIAHSHGLLSIPDGILLRPQDAMDLPAGKVAFLVSGTQG
HHHHHHCCEEEEECCHHHHHHHHHHHCCEEECCCCEEECCCCCCCCCCCCEEEEEECCCC
EPMSALSRVAVDNHKHVSVERGDTVVLSSRIIPGNEKGIFRMIDHMARRGADVMYGSMNP
CHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEECCCCC
PLHVSGHGSIEEMKLVLNLVRPRFFMPIHGEFRQLSKHARLAEHLRFAGLEETFVMDTGD
CEEECCCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCC
ILEFDHHGARKAGRVTVGHVCIDSGSVDDVVQEMVIRDRRLLSEDGIVLAIIAINRHTGK
EEECCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCC
LESPPEIVSRGFNVGIEGSAFMETAKAIVAKTLESSSQEEKTDWGVMKEKVRADLKRYIV
CCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KETSRRPLILPVIMEV
HHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]