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The map label for this gene is phnA [H]

Identifier: 116623467

GI number: 116623467

Start: 5539387

End: 5540520

Strand: Direct

Name: phnA [H]

Synonym: Acid_4377

Alternate gene names: 116623467

Gene position: 5539387-5540520 (Clockwise)

Preceding gene: 116623466

Following gene: 116623479

Centisome position: 55.58

GC content: 58.29

Gene sequence:

>1134_bases
ATGCGAACGGCTTTGGCGTTTGCCGTAATGGGGCTGTTGGCCGCTCTCTTGCCGGCCGCGGAAAAGCGAATCACCGTCAA
CGGAAAGAAGTATGACTGGCCCAAGTCGCCGGTGGTCGTGATCCTGATTGACGGCGGAGACCCGGCCTATGTGAATGCCG
CGCTTGCCCAGGGGCTGCTACCCAACTTCAAGAAGATCATGACCGAGGGGTTTGTTTCCATCGCCGTGGGAGCGATGCCC
AGCTTCACCAATCCGAATAACATCTCGGTGATCACTGGAGTGCCCCCTTCCGTGCACGGAATCTCAGGGAATTTCTTCCT
GAACCCCGCCACCGGCCAGGAAACTATGATGGATCAACCACAGTTCCTGCGGGCCGACAGCATTCTCGCGACCTTCTCGG
AGCACGGGGCCAAAGTTGTCGCCCTTACGGCAAAAGACAAGCTGGCCAAAATACTTGCTTATAAATTGCAGCATGGCATT
TCGTTCTCCGCCGAGAAGGCCGATCAGTGCACGAAACAGAACAACGGCATCGATAACTGCCTCGCCTATGTCGGCAGGCC
CCTGCCCAGCGCATACTCCGCCGATCTGTCGCTGTTCGTTCTGGAGGCGGGCATCCGGATACTGGAGCGGGAAAAGCCGG
ACCTGATGTATCTCTCGCTTTCCGACTACATTCAACACAAGTACCCCCCGGGCTCCAAAGAAGCAAACGCGTACTACGTC
GGAATTGATGATGCGCTGGGACGGTTGCTCGCGCTTGGCGCGACTGTCGCCCTAACGGCGGACCACGGCATGAACGATAA
ATCGAACGCCGACGGGTCTCCCAATATCGTGTTTCTGCAAGACCTGCTCGACATGCAATTCGGGAGCGGCACGGCCAAGG
TCATCCTGCCCATCACAGACCCATACGTGAAGCATCATGGCGCGCTCGGTTCTTACGCCACGATCTTTTTCCAGCAGCCG
GTTTCCGCGCCCGCCGTGATGAGTCTTCTGAACCGGCAGCCTGGTGTCGAACTCGTGATGGATCGTGCGGCCGCCGCCAA
ACTGTTCGATCTGCCCGCGGACCGCATCGGGACCTGGTTGTCGTCGCCGATCGAGGCACTGTCTTGGGTAAGAGTAGGGC
TACTCTCGATCTGA

Upstream 100 bases:

>100_bases
GATGAGCGTCGCATCTCTATCGCATGCAAAGCTGATGCGAGCCATCGAACTCCTCGGTTCCCGGGTGGCGCCAGCTCTTG
GGATTGCTAGAATGTCCTAA

Downstream 100 bases:

>100_bases
CCGAGCTCGGCGGAATGCGCTTGCGCTCTCACGGCGGACTGGCGGACTGCAACGTCTACCTGATGTTTTCGCGGCCGTTA
AACGATCGGTACGCCGCGAT

Product: phosphonoacetate hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MRTALAFAVMGLLAALLPAAEKRITVNGKKYDWPKSPVVVILIDGGDPAYVNAALAQGLLPNFKKIMTEGFVSIAVGAMP
SFTNPNNISVITGVPPSVHGISGNFFLNPATGQETMMDQPQFLRADSILATFSEHGAKVVALTAKDKLAKILAYKLQHGI
SFSAEKADQCTKQNNGIDNCLAYVGRPLPSAYSADLSLFVLEAGIRILEREKPDLMYLSLSDYIQHKYPPGSKEANAYYV
GIDDALGRLLALGATVALTADHGMNDKSNADGSPNIVFLQDLLDMQFGSGTAKVILPITDPYVKHHGALGSYATIFFQQP
VSAPAVMSLLNRQPGVELVMDRAAAAKLFDLPADRIGTWLSSPIEALSWVRVGLLSI

Sequences:

>Translated_377_residues
MRTALAFAVMGLLAALLPAAEKRITVNGKKYDWPKSPVVVILIDGGDPAYVNAALAQGLLPNFKKIMTEGFVSIAVGAMP
SFTNPNNISVITGVPPSVHGISGNFFLNPATGQETMMDQPQFLRADSILATFSEHGAKVVALTAKDKLAKILAYKLQHGI
SFSAEKADQCTKQNNGIDNCLAYVGRPLPSAYSADLSLFVLEAGIRILEREKPDLMYLSLSDYIQHKYPPGSKEANAYYV
GIDDALGRLLALGATVALTADHGMNDKSNADGSPNIVFLQDLLDMQFGSGTAKVILPITDPYVKHHGALGSYATIFFQQP
VSAPAVMSLLNRQPGVELVMDRAAAAKLFDLPADRIGTWLSSPIEALSWVRVGLLSI
>Mature_377_residues
MRTALAFAVMGLLAALLPAAEKRITVNGKKYDWPKSPVVVILIDGGDPAYVNAALAQGLLPNFKKIMTEGFVSIAVGAMP
SFTNPNNISVITGVPPSVHGISGNFFLNPATGQETMMDQPQFLRADSILATFSEHGAKVVALTAKDKLAKILAYKLQHGI
SFSAEKADQCTKQNNGIDNCLAYVGRPLPSAYSADLSLFVLEAGIRILEREKPDLMYLSLSDYIQHKYPPGSKEANAYYV
GIDDALGRLLALGATVALTADHGMNDKSNADGSPNIVFLQDLLDMQFGSGTAKVILPITDPYVKHHGALGSYATIFFQQP
VSAPAVMSLLNRQPGVELVMDRAAAAKLFDLPADRIGTWLSSPIEALSWVRVGLLSI

Specific function: Specifically hydrolyzes phosphonoacetate. Does not have activity on other organophosphonates or acetates [H]

COG id: COG1524

COG function: function code R; Uncharacterized proteins of the AP superfamily

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family. PhnA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR002591
- InterPro:   IPR012710
- InterPro:   IPR023116 [H]

Pfam domain/function: PF01663 Phosphodiest [H]

EC number: =3.11.1.2 [H]

Molecular weight: Translated: 40373; Mature: 40373

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTALAFAVMGLLAALLPAAEKRITVNGKKYDWPKSPVVVILIDGGDPAYVNAALAQGLL
CCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCC
PNFKKIMTEGFVSIAVGAMPSFTNPNNISVITGVPPSVHGISGNFFLNPATGQETMMDQP
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCHHHCCCC
QFLRADSILATFSEHGAKVVALTAKDKLAKILAYKLQHGISFSAEKADQCTKQNNGIDNC
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
LAYVGRPLPSAYSADLSLFVLEAGIRILEREKPDLMYLSLSDYIQHKYPPGSKEANAYYV
HHHHCCCCCCCHHCCCEEEEHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCEEEE
GIDDALGRLLALGATVALTADHGMNDKSNADGSPNIVFLQDLLDMQFGSGTAKVILPITD
ECHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEEECCC
PYVKHHGALGSYATIFFQQPVSAPAVMSLLNRQPGVELVMDRAAAAKLFDLPADRIGTWL
HHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
SSPIEALSWVRVGLLSI
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTALAFAVMGLLAALLPAAEKRITVNGKKYDWPKSPVVVILIDGGDPAYVNAALAQGLL
CCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCC
PNFKKIMTEGFVSIAVGAMPSFTNPNNISVITGVPPSVHGISGNFFLNPATGQETMMDQP
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCHHHCCCC
QFLRADSILATFSEHGAKVVALTAKDKLAKILAYKLQHGISFSAEKADQCTKQNNGIDNC
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHH
LAYVGRPLPSAYSADLSLFVLEAGIRILEREKPDLMYLSLSDYIQHKYPPGSKEANAYYV
HHHHCCCCCCCHHCCCEEEEHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCEEEE
GIDDALGRLLALGATVALTADHGMNDKSNADGSPNIVFLQDLLDMQFGSGTAKVILPITD
ECHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCEEEEEECCC
PYVKHHGALGSYATIFFQQPVSAPAVMSLLNRQPGVELVMDRAAAAKLFDLPADRIGTWL
HHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
SSPIEALSWVRVGLLSI
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA