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The map label for this gene is icfG [H]

Identifier: 116623386

GI number: 116623386

Start: 5441250

End: 5442437

Strand: Direct

Name: icfG [H]

Synonym: Acid_4295

Alternate gene names: 116623386

Gene position: 5441250-5442437 (Clockwise)

Preceding gene: 116623385

Following gene: 116623387

Centisome position: 54.6

GC content: 62.71

Gene sequence:

>1188_bases
ATGAGTGCGACCGTAGAGCAGACGCCACGCCAGATATTGGTGGTGGATGATGAGCCGGATCTGGAGTTGCTGGTGCGGCA
GCGGTTTCGCCGTCAGATTCGCGAGGGAGAGTTCGAGTTTTTCTTTAGCCACAACGGCGAAGAGGCGCTGGTAGCGCTGG
ATGGGAAGCCCACCATCGACGTGGTGTTAAGCGACATCAACATGCCTGTGATGGACGGGCTGACGCTGCTGGGCAAGCTG
CGGGAGCGGCAGGGGTGCCTGAAGGCGGTGGTGGTGTCGGCGTACGGCGATATGCCGAATATACGCACGGCGATGAATCG
CGGGGCGATCGATTTCCTGATGAAGCCGATCGATTTCGAGGACCTGGAAATCACGATTCGCAAGACGCTGGATCACGTGG
CGGCGATCCGGCAATCGATGGCGGTGCAGGAGAGCTTGCTGGCGATCCGGCAGGAGCTGAGCGTGGCGGCGCGGATTCAG
CAATCGATATTGCCCCGGACGTTCCCGCCATTTCCCGCGCGCAAGGATTTCGAGCTGCACGCCTCCATGATCCCGGCGAA
GGAAGTGGGCGGCGACCTTTTCGATTTCTTCCTGCTGGACGAAGATCACCTCGGCCTGGTGCTGGGCGATGTGAGCGGCA
AGGGCGTTCCGGCGGCGCTGTTCATGGCGGTATCGCGCACGCTGCTGCGCGCCACGGCGCTGCAGAAATTGCCGCCCGGA
GAGTGCCTGCAATACGTGAATACGACCCTGGCGGCGCAGAATCCCTCGCACATGTTCGTGACCATGTTCTACGGGATTTT
GAACACGCGCACGGGCGAGCTGCAATTCGCCAACGGGGGACACAATCCGCCGTACGTGTTTTCGGCCGATGGAAAGGTGC
GGGAGCTGCCGCGCAACAAGAACGGCATGATCGTGGGGCTGCTGGATGGGGCGCCTTACCAGACCGCGAGCCACACGATC
GCGCCCGGCGAAGCGCTGCTGGTCTATACCGACGGGGTAACGGAGGCGATGGACCGCAGGGAGGATTTCTACGGCGAGGA
GCGGCTGGAGGCTCTGTTGCGAGAAAACGGCGCGCTGCCGGTGGACGCGCTGGTGAAGCGGCTGATGGCGAGCGTGCAGG
AGTTCGCGGGCGGGGCCGAGCAATCCGACGACATCACGGTGATGGCAATGCGCTACCGGGGGAATTGA

Upstream 100 bases:

>100_bases
GAGTCCATGCTTACCTTGGATACCCAGGGTTTTCCGGGCCGTGTTGCCTTAAGTAGTAGATTCGGATAAGCTCAGAAATC
CCGATTGGGCCAAGGAATCT

Downstream 100 bases:

>100_bases
GAGGATGGCGAAAGTCCTGCTGGTGGAAGATAACGAGATGAACCGCGATATGCTGTCGCGGCGGTTGATGCGCCAGGGCT
TCGAAGTCACGGTGGCGGTG

Product: response regulator receiver modulated serine phosphatase

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSATVEQTPRQILVVDDEPDLELLVRQRFRRQIREGEFEFFFSHNGEEALVALDGKPTIDVVLSDINMPVMDGLTLLGKL
RERQGCLKAVVVSAYGDMPNIRTAMNRGAIDFLMKPIDFEDLEITIRKTLDHVAAIRQSMAVQESLLAIRQELSVAARIQ
QSILPRTFPPFPARKDFELHASMIPAKEVGGDLFDFFLLDEDHLGLVLGDVSGKGVPAALFMAVSRTLLRATALQKLPPG
ECLQYVNTTLAAQNPSHMFVTMFYGILNTRTGELQFANGGHNPPYVFSADGKVRELPRNKNGMIVGLLDGAPYQTASHTI
APGEALLVYTDGVTEAMDRREDFYGEERLEALLRENGALPVDALVKRLMASVQEFAGGAEQSDDITVMAMRYRGN

Sequences:

>Translated_395_residues
MSATVEQTPRQILVVDDEPDLELLVRQRFRRQIREGEFEFFFSHNGEEALVALDGKPTIDVVLSDINMPVMDGLTLLGKL
RERQGCLKAVVVSAYGDMPNIRTAMNRGAIDFLMKPIDFEDLEITIRKTLDHVAAIRQSMAVQESLLAIRQELSVAARIQ
QSILPRTFPPFPARKDFELHASMIPAKEVGGDLFDFFLLDEDHLGLVLGDVSGKGVPAALFMAVSRTLLRATALQKLPPG
ECLQYVNTTLAAQNPSHMFVTMFYGILNTRTGELQFANGGHNPPYVFSADGKVRELPRNKNGMIVGLLDGAPYQTASHTI
APGEALLVYTDGVTEAMDRREDFYGEERLEALLRENGALPVDALVKRLMASVQEFAGGAEQSDDITVMAMRYRGN
>Mature_394_residues
SATVEQTPRQILVVDDEPDLELLVRQRFRRQIREGEFEFFFSHNGEEALVALDGKPTIDVVLSDINMPVMDGLTLLGKLR
ERQGCLKAVVVSAYGDMPNIRTAMNRGAIDFLMKPIDFEDLEITIRKTLDHVAAIRQSMAVQESLLAIRQELSVAARIQQ
SILPRTFPPFPARKDFELHASMIPAKEVGGDLFDFFLLDEDHLGLVLGDVSGKGVPAALFMAVSRTLLRATALQKLPPGE
CLQYVNTTLAAQNPSHMFVTMFYGILNTRTGELQFANGGHNPPYVFSADGKVRELPRNKNGMIVGLLDGAPYQTASHTIA
PGEALLVYTDGVTEAMDRREDFYGEERLEALLRENGALPVDALVKRLMASVQEFAGGAEQSDDITVMAMRYRGN

Specific function: Involved in cross-regulation of inorganic carbon and glucose metabolisms [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=132, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788550, Length=147, Percent_Identity=29.9319727891156, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=125, Percent_Identity=29.6, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003660
- InterPro:   IPR001932
- InterPro:   IPR010822 [H]

Pfam domain/function: PF00672 HAMP; PF07228 SpoIIE [H]

EC number: NA

Molecular weight: Translated: 43726; Mature: 43595

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATVEQTPRQILVVDDEPDLELLVRQRFRRQIREGEFEFFFSHNGEEALVALDGKPTID
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCEE
VVLSDINMPVMDGLTLLGKLRERQGCLKAVVVSAYGDMPNIRTAMNRGAIDFLMKPIDFE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHCCCCCC
DLEITIRKTLDHVAAIRQSMAVQESLLAIRQELSVAARIQQSILPRTFPPFPARKDFELH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
ASMIPAKEVGGDLFDFFLLDEDHLGLVLGDVSGKGVPAALFMAVSRTLLRATALQKLPPG
HHCCCHHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
ECLQYVNTTLAAQNPSHMFVTMFYGILNTRTGELQFANGGHNPPYVFSADGKVRELPRNK
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCHHHCCCCC
NGMIVGLLDGAPYQTASHTIAPGEALLVYTDGVTEAMDRREDFYGEERLEALLRENGALP
CCEEEEEECCCCCCCCCCEECCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
VDALVKRLMASVQEFAGGAEQSDDITVMAMRYRGN
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SATVEQTPRQILVVDDEPDLELLVRQRFRRQIREGEFEFFFSHNGEEALVALDGKPTID
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEECCCCCEE
VVLSDINMPVMDGLTLLGKLRERQGCLKAVVVSAYGDMPNIRTAMNRGAIDFLMKPIDFE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHCCCCCC
DLEITIRKTLDHVAAIRQSMAVQESLLAIRQELSVAARIQQSILPRTFPPFPARKDFELH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
ASMIPAKEVGGDLFDFFLLDEDHLGLVLGDVSGKGVPAALFMAVSRTLLRATALQKLPPG
HHCCCHHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
ECLQYVNTTLAAQNPSHMFVTMFYGILNTRTGELQFANGGHNPPYVFSADGKVRELPRNK
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCHHHCCCCC
NGMIVGLLDGAPYQTASHTIAPGEALLVYTDGVTEAMDRREDFYGEERLEALLRENGALP
CCEEEEEECCCCCCCCCCEECCCCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHCCCCC
VDALVKRLMASVQEFAGGAEQSDDITVMAMRYRGN
HHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8075401; 8905231 [H]