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The map label for this gene is gacS [H]

Identifier: 116623382

GI number: 116623382

Start: 5435627

End: 5438017

Strand: Reverse

Name: gacS [H]

Synonym: Acid_4291

Alternate gene names: 116623382

Gene position: 5438017-5435627 (Counterclockwise)

Preceding gene: 116623383

Following gene: 116623380

Centisome position: 54.57

GC content: 65.33

Gene sequence:

>2391_bases
ATGACCATACGGCGCCGCCTGACGCTCTCGTTCTTCGCCATCCTCTGCCTCCTCGGCCTCAACCTCGTCATCTATTTCTG
GAGCGACATCAAGCGCAAATCCACCTTCGAGGAAATGCGCAGCGCCATCTCCCGCCAGATCCTCATCTCCAGCGTGCAGC
AGGATCTCAGCGATTTCCAGAAGCAGGTCACCCTGCTCAGCCAGATCTCCGCCGACGCCGGTTCCAGCGGCGGCGCCTCC
CCCGAAGACATCGCTCAATTCAACTCGCGCCTCGATCTCATCGCATCCCAGATCCACGAGGTCCGCTCCCAAACCCCCAA
GGCCGAAGCCGCCAAAGTTGACGATTTCGCCGCCGCCTTCCAGGAACTCAGCGCCTCCTGGCGCATCTTCTACCAGAACC
TCGGCCGCAACCAATCCAAAGCCATCACCGAAGTCGTCATGCACGCCGAGCCCCTCGGCCAGAAGGTCATGCAGCAGATG
CTCCCCGACCTGCAGAAGGACGAGCGCGACCTCGTCTCCAAAGCCAGCGCCCACTTCTACGACGCCGCCAGCTTCACCGG
CCAGGTCACCGTCGGCATCTTCCTGCTCTCCGGCCTTCTCGCCGCCGGACTCGCCTTCATCGTCTCGCGCCACCTCACCC
GCGGCGTCAGCGCCCTCAAAGCCGGCGCCGATACCCTGGGCGCGGGCGACCTCGAATCTCACATCGAGCTCCCCGGCCAC
GACGAGCTCGCCGAGCTCGCCCGCGCCTTCAACGGCATGACCGGCCGCCTGCGCCTCGCCCGCCAGGACCTCACCCGCGC
CAACGCCGAGCTCGAACAGCGCCACCATGAGCTCCAGCTCCTAATGGAAGCCGCCGAGAGCGCCAACCAGGCCAAGAGCC
AGTTCCTCGCCAACATGAGCCACGAGCTGCGCACCCCCATGAACGCCATCATCGGCTATAGCGAAATGCTCATGGATGAA
GCCCAGGACATGGCCAGCGCGTCCGCCCTGCCCGAAGTCGAGGCCGTCTTCGTCCCCGAGCTCAAGAAGATCACCGCCGC
CGGCCGCCACCTCCTCGCCCTCATCAATGACATTCTCGACCTCTCCAAAATCGAGGCCGGGAAAATGGACCTCTACCTCG
AAACCTTCCAGATCCCCGAAATGGTCAACGACGTCGCCTGCACCATCCAGCCCCTCGTCGATAAAAACGCCAACCGCCTG
CAAATCGATATCTCCCCCGGCATCGGCCCCATGCGCGCCGACCTCACCAAGGTCCGCCAGGTCCTCTTCAACCTGCTCAG
CAACGCCAGCAAGTTCACCGACAAGGGCGTTATCGAGCTCCGCGTCTTCAACCACCACGACGAAGCCCGCGATTGGATCG
CCTTCCAGGTCAAGGATTCCGGCATCGGCATGACCCGCGAGCAGGCCGCCCGCATCTTCGAAGCCTTCACCCAGGCCGAC
GCCTCCACCACCCGCAAGTACGGCGGCACCGGACTCGGCCTCACCATCACCCGCAAATTCTGCGAGCTCATGGGCGGCCG
CATCACCGTGGATAGCGAGCCGGGGCAGGGAACTGTCTTCACCGTCCGCCTGCCCCTCGAGGTCGCCGCCGACGCGCACA
AATCCGCCGGCCAGGAACCCGACCGCGCCATGCCCGAGGCCGAGCACGCCGCCGCCACCATCCTCGTCATCGACGACGAC
CCGGTGATCGGCGACCTCATGAAGAGCTTCCTCGGCAAAGAGGGCTATCACGTCATCGTCGCCCCCGGCGGCGAGGAAGG
CATCGCCGAAGCCCGCAAGCTCCGCCCCGACGTCATCACCCTCGATGTCGCCATGCCCCGCATGGATGGCTGGACCGTCC
TCGGCGCCCTCAAAGCCGACCCCGCCCTCGCCGACATCCCCGTCATCATGCTCACTATGGTCGATAACAAGAGCATGGGC
TACGCCCTCGGCGCCTCCGACTACATGACCAAGCCGCTCAACCGGGAGCGCCTCGCCGCCGTCCTCGCCAAGTACAGCCG
CCTGCGCGGCCGCCACCCCGTCCTCGTCGTCGAAGACGATGCCGATACCCGCGCCATCCTCAAGACCACGCTCGAAAAGG
ATGGCTGGCTCGTGGACGAAGCCGAGAACGGCCGCGTCGCCCTCGAGCTCGCCCGCGCCGCCGTCCCCAGCCTCGTCCTC
CTCGACCTCATGATGCCCGAGATGGATGGCTTCGCCTTCCTCGAGGAATTCCGCCGCATCGCCAACGCCCGCAGCATCCC
GGTCATCGTCCTCACCGCCAAGGACCTCACCGCCGACGACCGCCGCCGCCTCAACGGCTACGTCGAACGCATCGTTCTGA
AAGGTTCCAACTCCGAATCGTTGCTCTCCCAGGTTCGCGCGCTAGTTGCCCAGACCTGCACTCCCACCTAG

Upstream 100 bases:

>100_bases
CGTCGATTCCACCCAGGTGCCCAAAGGACTGAAGACCCTCAAGATCGATGGAAAACTGCCCGGCGATAAAGGTTACCCGC
TGCACTAAAAGGCCGAACGA

Downstream 100 bases:

>100_bases
CTCCCGCAACTTCCCACGCGCCTCCGCAATCTCCCTCTGCAACTGCGCCTTATGCCGGAAGGTCTGCGAGTCCACACTGG
TCCCCGGTGATGCCAGCTCC

Product: integral membrane sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 796; Mature: 795

Protein sequence:

>796_residues
MTIRRRLTLSFFAILCLLGLNLVIYFWSDIKRKSTFEEMRSAISRQILISSVQQDLSDFQKQVTLLSQISADAGSSGGAS
PEDIAQFNSRLDLIASQIHEVRSQTPKAEAAKVDDFAAAFQELSASWRIFYQNLGRNQSKAITEVVMHAEPLGQKVMQQM
LPDLQKDERDLVSKASAHFYDAASFTGQVTVGIFLLSGLLAAGLAFIVSRHLTRGVSALKAGADTLGAGDLESHIELPGH
DELAELARAFNGMTGRLRLARQDLTRANAELEQRHHELQLLMEAAESANQAKSQFLANMSHELRTPMNAIIGYSEMLMDE
AQDMASASALPEVEAVFVPELKKITAAGRHLLALINDILDLSKIEAGKMDLYLETFQIPEMVNDVACTIQPLVDKNANRL
QIDISPGIGPMRADLTKVRQVLFNLLSNASKFTDKGVIELRVFNHHDEARDWIAFQVKDSGIGMTREQAARIFEAFTQAD
ASTTRKYGGTGLGLTITRKFCELMGGRITVDSEPGQGTVFTVRLPLEVAADAHKSAGQEPDRAMPEAEHAAATILVIDDD
PVIGDLMKSFLGKEGYHVIVAPGGEEGIAEARKLRPDVITLDVAMPRMDGWTVLGALKADPALADIPVIMLTMVDNKSMG
YALGASDYMTKPLNRERLAAVLAKYSRLRGRHPVLVVEDDADTRAILKTTLEKDGWLVDEAENGRVALELARAAVPSLVL
LDLMMPEMDGFAFLEEFRRIANARSIPVIVLTAKDLTADDRRRLNGYVERIVLKGSNSESLLSQVRALVAQTCTPT

Sequences:

>Translated_796_residues
MTIRRRLTLSFFAILCLLGLNLVIYFWSDIKRKSTFEEMRSAISRQILISSVQQDLSDFQKQVTLLSQISADAGSSGGAS
PEDIAQFNSRLDLIASQIHEVRSQTPKAEAAKVDDFAAAFQELSASWRIFYQNLGRNQSKAITEVVMHAEPLGQKVMQQM
LPDLQKDERDLVSKASAHFYDAASFTGQVTVGIFLLSGLLAAGLAFIVSRHLTRGVSALKAGADTLGAGDLESHIELPGH
DELAELARAFNGMTGRLRLARQDLTRANAELEQRHHELQLLMEAAESANQAKSQFLANMSHELRTPMNAIIGYSEMLMDE
AQDMASASALPEVEAVFVPELKKITAAGRHLLALINDILDLSKIEAGKMDLYLETFQIPEMVNDVACTIQPLVDKNANRL
QIDISPGIGPMRADLTKVRQVLFNLLSNASKFTDKGVIELRVFNHHDEARDWIAFQVKDSGIGMTREQAARIFEAFTQAD
ASTTRKYGGTGLGLTITRKFCELMGGRITVDSEPGQGTVFTVRLPLEVAADAHKSAGQEPDRAMPEAEHAAATILVIDDD
PVIGDLMKSFLGKEGYHVIVAPGGEEGIAEARKLRPDVITLDVAMPRMDGWTVLGALKADPALADIPVIMLTMVDNKSMG
YALGASDYMTKPLNRERLAAVLAKYSRLRGRHPVLVVEDDADTRAILKTTLEKDGWLVDEAENGRVALELARAAVPSLVL
LDLMMPEMDGFAFLEEFRRIANARSIPVIVLTAKDLTADDRRRLNGYVERIVLKGSNSESLLSQVRALVAQTCTPT
>Mature_795_residues
TIRRRLTLSFFAILCLLGLNLVIYFWSDIKRKSTFEEMRSAISRQILISSVQQDLSDFQKQVTLLSQISADAGSSGGASP
EDIAQFNSRLDLIASQIHEVRSQTPKAEAAKVDDFAAAFQELSASWRIFYQNLGRNQSKAITEVVMHAEPLGQKVMQQML
PDLQKDERDLVSKASAHFYDAASFTGQVTVGIFLLSGLLAAGLAFIVSRHLTRGVSALKAGADTLGAGDLESHIELPGHD
ELAELARAFNGMTGRLRLARQDLTRANAELEQRHHELQLLMEAAESANQAKSQFLANMSHELRTPMNAIIGYSEMLMDEA
QDMASASALPEVEAVFVPELKKITAAGRHLLALINDILDLSKIEAGKMDLYLETFQIPEMVNDVACTIQPLVDKNANRLQ
IDISPGIGPMRADLTKVRQVLFNLLSNASKFTDKGVIELRVFNHHDEARDWIAFQVKDSGIGMTREQAARIFEAFTQADA
STTRKYGGTGLGLTITRKFCELMGGRITVDSEPGQGTVFTVRLPLEVAADAHKSAGQEPDRAMPEAEHAAATILVIDDDP
VIGDLMKSFLGKEGYHVIVAPGGEEGIAEARKLRPDVITLDVAMPRMDGWTVLGALKADPALADIPVIMLTMVDNKSMGY
ALGASDYMTKPLNRERLAAVLAKYSRLRGRHPVLVVEDDADTRAILKTTLEKDGWLVDEAENGRVALELARAAVPSLVLL
DLMMPEMDGFAFLEEFRRIANARSIPVIVLTAKDLTADDRRRLNGYVERIVLKGSNSESLLSQVRALVAQTCTPT

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=606, Percent_Identity=26.5676567656766, Blast_Score=183, Evalue=4e-47,
Organism=Escherichia coli, GI145693157, Length=493, Percent_Identity=30.2231237322515, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI87081816, Length=513, Percent_Identity=30.7992202729045, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788713, Length=393, Percent_Identity=31.8066157760814, Blast_Score=165, Evalue=1e-41,
Organism=Escherichia coli, GI48994928, Length=388, Percent_Identity=30.6701030927835, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1786600, Length=260, Percent_Identity=29.6153846153846, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788393, Length=318, Percent_Identity=25.7861635220126, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786912, Length=250, Percent_Identity=27.6, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1786783, Length=293, Percent_Identity=24.2320819112628, Blast_Score=83, Evalue=9e-17,
Organism=Escherichia coli, GI1790346, Length=309, Percent_Identity=24.5954692556634, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788549, Length=313, Percent_Identity=26.517571884984, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87082128, Length=239, Percent_Identity=28.8702928870293, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=27.8481012658228, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1787894, Length=322, Percent_Identity=24.5341614906832, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082052, Length=140, Percent_Identity=30, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=33.8983050847458, Blast_Score=66, Evalue=8e-12,
Organism=Escherichia coli, GI1789808, Length=334, Percent_Identity=23.3532934131737, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786599, Length=116, Percent_Identity=31.8965517241379, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=82, Percent_Identity=37.8048780487805, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 87290; Mature: 87159

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50885 HAMP ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRRRLTLSFFAILCLLGLNLVIYFWSDIKRKSTFEEMRSAISRQILISSVQQDLSDFQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQVTLLSQISADAGSSGGASPEDIAQFNSRLDLIASQIHEVRSQTPKAEAAKVDDFAAAF
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QELSASWRIFYQNLGRNQSKAITEVVMHAEPLGQKVMQQMLPDLQKDERDLVSKASAHFY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHH
DAASFTGQVTVGIFLLSGLLAAGLAFIVSRHLTRGVSALKAGADTLGAGDLESHIELPGH
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCC
DELAELARAFNGMTGRLRLARQDLTRANAELEQRHHELQLLMEAAESANQAKSQFLANMS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HELRTPMNAIIGYSEMLMDEAQDMASASALPEVEAVFVPELKKITAAGRHLLALINDILD
HHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
LSKIEAGKMDLYLETFQIPEMVNDVACTIQPLVDKNANRLQIDISPGIGPMRADLTKVRQ
HHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
VLFNLLSNASKFTDKGVIELRVFNHHDEARDWIAFQVKDSGIGMTREQAARIFEAFTQAD
HHHHHHHCHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCC
ASTTRKYGGTGLGLTITRKFCELMGGRITVDSEPGQGTVFTVRLPLEVAADAHKSAGQEP
HHHHHHCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCC
DRAMPEAEHAAATILVIDDDPVIGDLMKSFLGKEGYHVIVAPGGEEGIAEARKLRPDVIT
CCCCCCCHHCEEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEE
LDVAMPRMDGWTVLGALKADPALADIPVIMLTMVDNKSMGYALGASDYMTKPLNRERLAA
EEEECCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCEEECCHHHHCCCCCHHHHHH
VLAKYSRLRGRHPVLVVEDDADTRAILKTTLEKDGWLVDEAENGRVALELARAAVPSLVL
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCCEEHHHHHHHHHHHHHH
LDLMMPEMDGFAFLEEFRRIANARSIPVIVLTAKDLTADDRRRLNGYVERIVLKGSNSES
HHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHH
LLSQVRALVAQTCTPT
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIRRRLTLSFFAILCLLGLNLVIYFWSDIKRKSTFEEMRSAISRQILISSVQQDLSDFQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQVTLLSQISADAGSSGGASPEDIAQFNSRLDLIASQIHEVRSQTPKAEAAKVDDFAAAF
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QELSASWRIFYQNLGRNQSKAITEVVMHAEPLGQKVMQQMLPDLQKDERDLVSKASAHFY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHCHHHHHHHHHHHHHHH
DAASFTGQVTVGIFLLSGLLAAGLAFIVSRHLTRGVSALKAGADTLGAGDLESHIELPGH
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCCC
DELAELARAFNGMTGRLRLARQDLTRANAELEQRHHELQLLMEAAESANQAKSQFLANMS
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HELRTPMNAIIGYSEMLMDEAQDMASASALPEVEAVFVPELKKITAAGRHLLALINDILD
HHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
LSKIEAGKMDLYLETFQIPEMVNDVACTIQPLVDKNANRLQIDISPGIGPMRADLTKVRQ
HHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
VLFNLLSNASKFTDKGVIELRVFNHHDEARDWIAFQVKDSGIGMTREQAARIFEAFTQAD
HHHHHHHCHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCC
ASTTRKYGGTGLGLTITRKFCELMGGRITVDSEPGQGTVFTVRLPLEVAADAHKSAGQEP
HHHHHHCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECHHHHHHHHHHCCCCC
DRAMPEAEHAAATILVIDDDPVIGDLMKSFLGKEGYHVIVAPGGEEGIAEARKLRPDVIT
CCCCCCCHHCEEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEE
LDVAMPRMDGWTVLGALKADPALADIPVIMLTMVDNKSMGYALGASDYMTKPLNRERLAA
EEEECCCCCCCEEEEECCCCCCHHCCCEEEEEEECCCCCCEEECCHHHHCCCCCHHHHHH
VLAKYSRLRGRHPVLVVEDDADTRAILKTTLEKDGWLVDEAENGRVALELARAAVPSLVL
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCCEEHHHHHHHHHHHHHH
LDLMMPEMDGFAFLEEFRRIANARSIPVIVLTAKDLTADDRRRLNGYVERIVLKGSNSES
HHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHH
LLSQVRALVAQTCTPT
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]