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The map label for this gene is clpB [H]

Identifier: 116623353

GI number: 116623353

Start: 5394215

End: 5395393

Strand: Direct

Name: clpB [H]

Synonym: Acid_4262

Alternate gene names: 116623353

Gene position: 5394215-5395393 (Clockwise)

Preceding gene: 116623351

Following gene: 116623354

Centisome position: 54.13

GC content: 61.07

Gene sequence:

>1179_bases
ATGGTACGCACAAAACAACTGCTGAATCCAACCCAGACCGGACGTGAATCGGAGCGTCTGGAAAGCGATCTTCGGAAGCG
TGTCATCGGTCAGGACGAGGCTATCCAACAGGTCCTGCGCATTTATCAGACTCACCTTGCAGGCCTCTCCAGCGCCGGCC
GGCCTGTGGGAACCCTGCTCTTCCTTGGGCCCACCGGCTCCGGCAAGACACGCCTGGTCGAAGCCGTGGCCGAAAGCCTG
GTCGGGGACGCGCGCGCGGTGATCAAAATCAACTGCGCCGAGTTCCAGCACAGCCATGAAATCGCCAAACTGATCGGCTC
GCCTCCCGGCTACCTGGGACATCGTGAGACCCACGCCGTCCTCCGCCAGGAAACCCTGAACCAATATCACACCGAAAAGG
TGAAGCTCAGCTTCGTGCTCTTTGACGAAATCGAGAAGGCCAGCGATACCCTCTGGAACCTCCTCCTCGGAATTCTGGAC
AAGGCCACCCTCACCACCGGCGACAACAACAAGGTCGATTTCTCCCAGGCCCTGATCTTCATGACCGGTAACCTGGGCGC
CGCCGAAATGAATTCCATCCTCAGCCCCACCCTCGGTTTCGCCGTCCTCCCCTCCCAGGAGCATCAGGCCCAGGGAGTGG
TCGATGGCGAGCTTGCGCAGAAACTCTCACGCGCCGCCGTCGAAGCCGCCCGCCGCAAGTTCACGCCGGAATTCATGAAC
CGCATCGATCGCACCGTGGTCTTTCGCCCGCTGGGCATCGTCCAGCTCCGCAAGATCCTCGCGCTCGAGCTCAATGACGT
GCAGCGCCGCATTTTCAACGCGCCCAATTCCATCCCCTTCGTGGTCACCGTCACCGAACCGGCCCGCGATTACCTTCTCG
AGCAGGGGACCGACATGAAATACGGCGCCCGCCATTTGAAGCGCGCCGTCGATCGCAACATCGTCCAACCCATCTCTAAC
CTGATCGCTACCGGACAGATTCGTAGCGGCGATCTCATCCGCGTCGATTACGATACCGCCCAGTCTTCCCTCACCTTCCT
CAAGGAGTGGGAGGACATGCCCGCCGTCGTCATGGCCGATCTGGTTGATACCTCGACCTTGCCCCTCCCCGCCAGTCGCC
GCGCCGGCACGGCAGCGGATTTCAACAGCATCGCCAACGTCCGGATCATGAAGCGCTAA

Upstream 100 bases:

>100_bases
ATTTCTTGGGGTTACGCTCCGCAGCACACGCCTGCAGGCCCAAATTTTGAGCCACACGCTCGAACCTCAGCATCCGTGAG
TGCAGAGTGGGAGCGGGAAC

Downstream 100 bases:

>100_bases
CGGTGACCGCGGCCGCCGCCGTCAGCCGCGGCCGCACTTCGCCTGTCCCGATCGCGTATGCGAACCGCGACCGTTAGGGA
GCGATCCTTCATCTGATCCG

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MVRTKQLLNPTQTGRESERLESDLRKRVIGQDEAIQQVLRIYQTHLAGLSSAGRPVGTLLFLGPTGSGKTRLVEAVAESL
VGDARAVIKINCAEFQHSHEIAKLIGSPPGYLGHRETHAVLRQETLNQYHTEKVKLSFVLFDEIEKASDTLWNLLLGILD
KATLTTGDNNKVDFSQALIFMTGNLGAAEMNSILSPTLGFAVLPSQEHQAQGVVDGELAQKLSRAAVEAARRKFTPEFMN
RIDRTVVFRPLGIVQLRKILALELNDVQRRIFNAPNSIPFVVTVTEPARDYLLEQGTDMKYGARHLKRAVDRNIVQPISN
LIATGQIRSGDLIRVDYDTAQSSLTFLKEWEDMPAVVMADLVDTSTLPLPASRRAGTAADFNSIANVRIMKR

Sequences:

>Translated_392_residues
MVRTKQLLNPTQTGRESERLESDLRKRVIGQDEAIQQVLRIYQTHLAGLSSAGRPVGTLLFLGPTGSGKTRLVEAVAESL
VGDARAVIKINCAEFQHSHEIAKLIGSPPGYLGHRETHAVLRQETLNQYHTEKVKLSFVLFDEIEKASDTLWNLLLGILD
KATLTTGDNNKVDFSQALIFMTGNLGAAEMNSILSPTLGFAVLPSQEHQAQGVVDGELAQKLSRAAVEAARRKFTPEFMN
RIDRTVVFRPLGIVQLRKILALELNDVQRRIFNAPNSIPFVVTVTEPARDYLLEQGTDMKYGARHLKRAVDRNIVQPISN
LIATGQIRSGDLIRVDYDTAQSSLTFLKEWEDMPAVVMADLVDTSTLPLPASRRAGTAADFNSIANVRIMKR
>Mature_392_residues
MVRTKQLLNPTQTGRESERLESDLRKRVIGQDEAIQQVLRIYQTHLAGLSSAGRPVGTLLFLGPTGSGKTRLVEAVAESL
VGDARAVIKINCAEFQHSHEIAKLIGSPPGYLGHRETHAVLRQETLNQYHTEKVKLSFVLFDEIEKASDTLWNLLLGILD
KATLTTGDNNKVDFSQALIFMTGNLGAAEMNSILSPTLGFAVLPSQEHQAQGVVDGELAQKLSRAAVEAARRKFTPEFMN
RIDRTVVFRPLGIVQLRKILALELNDVQRRIFNAPNSIPFVVTVTEPARDYLLEQGTDMKYGARHLKRAVDRNIVQPISN
LIATGQIRSGDLIRVDYDTAQSSLTFLKEWEDMPAVVMADLVDTSTLPLPASRRAGTAADFNSIANVRIMKR

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=336, Percent_Identity=27.9761904761905, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI1788943, Length=323, Percent_Identity=34.3653250773994, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787109, Length=337, Percent_Identity=29.080118694362, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320464, Length=336, Percent_Identity=36.0119047619048, Blast_Score=199, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6323002, Length=331, Percent_Identity=30.5135951661631, Blast_Score=151, Evalue=1e-37,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 43373; Mature: 43373

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRTKQLLNPTQTGRESERLESDLRKRVIGQDEAIQQVLRIYQTHLAGLSSAGRPVGTLL
CCCCHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
FLGPTGSGKTRLVEAVAESLVGDARAVIKINCAEFQHSHEIAKLIGSPPGYLGHRETHAV
EEECCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LRQETLNQYHTEKVKLSFVLFDEIEKASDTLWNLLLGILDKATLTTGDNNKVDFSQALIF
HHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHEEEE
MTGNLGAAEMNSILSPTLGFAVLPSQEHQAQGVVDGELAQKLSRAAVEAARRKFTPEFMN
EECCCCHHHHHHHHCCCCCEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
RIDRTVVFRPLGIVQLRKILALELNDVQRRIFNAPNSIPFVVTVTEPARDYLLEQGTDMK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCCCCH
YGARHLKRAVDRNIVQPISNLIATGQIRSGDLIRVDYDTAQSSLTFLKEWEDMPAVVMAD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHH
LVDTSTLPLPASRRAGTAADFNSIANVRIMKR
HHCCCCCCCCCCCCCCCHHHHHHHCCEEEECC
>Mature Secondary Structure
MVRTKQLLNPTQTGRESERLESDLRKRVIGQDEAIQQVLRIYQTHLAGLSSAGRPVGTLL
CCCCHHHCCCHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
FLGPTGSGKTRLVEAVAESLVGDARAVIKINCAEFQHSHEIAKLIGSPPGYLGHRETHAV
EEECCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LRQETLNQYHTEKVKLSFVLFDEIEKASDTLWNLLLGILDKATLTTGDNNKVDFSQALIF
HHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEHHHEEEE
MTGNLGAAEMNSILSPTLGFAVLPSQEHQAQGVVDGELAQKLSRAAVEAARRKFTPEFMN
EECCCCHHHHHHHHCCCCCEEECCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
RIDRTVVFRPLGIVQLRKILALELNDVQRRIFNAPNSIPFVVTVTEPARDYLLEQGTDMK
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCCCCH
YGARHLKRAVDRNIVQPISNLIATGQIRSGDLIRVDYDTAQSSLTFLKEWEDMPAVVMAD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHCCHHHHHHH
LVDTSTLPLPASRRAGTAADFNSIANVRIMKR
HHCCCCCCCCCCCCCCCHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA