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The map label for this gene is zraS [H]

Identifier: 116623350

GI number: 116623350

Start: 5389907

End: 5390707

Strand: Direct

Name: zraS [H]

Synonym: Acid_4259

Alternate gene names: 116623350

Gene position: 5389907-5390707 (Clockwise)

Preceding gene: 116623349

Following gene: 116623351

Centisome position: 54.08

GC content: 62.55

Gene sequence:

>801_bases
ATGGAACACTTTCTCCAAACCCGTCCGCCGCGATCGGCGCCGGCTAACTCGGACTCCCGGAATAACGGGGTCTTTGACCC
GATACAATTGCTCGAAGTTACCGCGCACGATCTCCGCAATCCGATAAGCGGAATCCTCGCGGCAGGCCAATATCTCCTTG
AAGACGCCAACTCGGTCCTTGATGAACACCACTTGGCTCTGCTCCAATCGATCGATGCCTCCAGCCGCTCCATGCTGCGG
TTGATCGAGGACGCTCTTGAACTTTGCGGCATCGAATCCGGGCATTTACGACTCGCAATCGAAACCACGGACCTGCAGCC
CGTCATGAACCGCGCCTTATTGCTCAGCAACATGGTCGCCGACCTGAAGCAGATCCGTTTGGAAGTCGAGCACCGCCGCA
GCGCCCCCCTACCCGCCATCTGTGTTGACCCGGTGAGAATCCTCCACGCGCTCGATAGCCTGCTCCTGACCTCCATCAAA
CTCGCTCGTCGCGGAAGCAAAATCTCCATCGGGGCGGGTTCGCGCGGCGACCGCCTCACGGTCTGGGTGCGTACCGAAGG
TTTCGGTATATCCCCCGTCGCGCTCCGCTCCCTGTTCAACCCCTTCCGCAAGGGCCGCAACAACCGCCCCGGCGTGGAAG
GCGGCATCGCCCTGTCGCTCGCCAAGGTCGGCCGCATCATTGAGGCCCACGGCGGCGCCGTACGCGTCGAAGGCCGTGAG
GGCGCCGAGTTGTTGATCAAGCTGGCATTACCCCTGACCACCCGCTCCGCCGCCCGCAAAGGCGCTGCCGGCGCTCACTA
G

Upstream 100 bases:

>100_bases
AGGCTAACTTCCAAATAAATAATTCCCTCCAGGATTGATCATGTTACGATGGCAGACAGCACTGTCTCGGTTCTCGTGTG
GGGAATTCCAGAAATAAAAA

Downstream 100 bases:

>100_bases
CGGTAGACTCAGGTCACCACCCGAATAATTTCCATCACCTGCGCTCATCCCGGCATCCTTTCAATGCATCGTTTCCCCGT
GAACTATGGCACACCGTATT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MEHFLQTRPPRSAPANSDSRNNGVFDPIQLLEVTAHDLRNPISGILAAGQYLLEDANSVLDEHHLALLQSIDASSRSMLR
LIEDALELCGIESGHLRLAIETTDLQPVMNRALLLSNMVADLKQIRLEVEHRRSAPLPAICVDPVRILHALDSLLLTSIK
LARRGSKISIGAGSRGDRLTVWVRTEGFGISPVALRSLFNPFRKGRNNRPGVEGGIALSLAKVGRIIEAHGGAVRVEGRE
GAELLIKLALPLTTRSAARKGAAGAH

Sequences:

>Translated_266_residues
MEHFLQTRPPRSAPANSDSRNNGVFDPIQLLEVTAHDLRNPISGILAAGQYLLEDANSVLDEHHLALLQSIDASSRSMLR
LIEDALELCGIESGHLRLAIETTDLQPVMNRALLLSNMVADLKQIRLEVEHRRSAPLPAICVDPVRILHALDSLLLTSIK
LARRGSKISIGAGSRGDRLTVWVRTEGFGISPVALRSLFNPFRKGRNNRPGVEGGIALSLAKVGRIIEAHGGAVRVEGRE
GAELLIKLALPLTTRSAARKGAAGAH
>Mature_266_residues
MEHFLQTRPPRSAPANSDSRNNGVFDPIQLLEVTAHDLRNPISGILAAGQYLLEDANSVLDEHHLALLQSIDASSRSMLR
LIEDALELCGIESGHLRLAIETTDLQPVMNRALLLSNMVADLKQIRLEVEHRRSAPLPAICVDPVRILHALDSLLLTSIK
LARRGSKISIGAGSRGDRLTVWVRTEGFGISPVALRSLFNPFRKGRNNRPGVEGGIALSLAKVGRIIEAHGGAVRVEGRE
GAELLIKLALPLTTRSAARKGAAGAH

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=30.1369863013699, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081816, Length=232, Percent_Identity=27.1551724137931, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693157, Length=238, Percent_Identity=27.3109243697479, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786912, Length=226, Percent_Identity=26.9911504424779, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 28752; Mature: 28752

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHFLQTRPPRSAPANSDSRNNGVFDPIQLLEVTAHDLRNPISGILAAGQYLLEDANSVL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEHHLALLQSIDASSRSMLRLIEDALELCGIESGHLRLAIETTDLQPVMNRALLLSNMVA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
DLKQIRLEVEHRRSAPLPAICVDPVRILHALDSLLLTSIKLARRGSKISIGAGSRGDRLT
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEE
VWVRTEGFGISPVALRSLFNPFRKGRNNRPGVEGGIALSLAKVGRIIEAHGGAVRVEGRE
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GAELLIKLALPLTTRSAARKGAAGAH
CHHHHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MEHFLQTRPPRSAPANSDSRNNGVFDPIQLLEVTAHDLRNPISGILAAGQYLLEDANSVL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEHHLALLQSIDASSRSMLRLIEDALELCGIESGHLRLAIETTDLQPVMNRALLLSNMVA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
DLKQIRLEVEHRRSAPLPAICVDPVRILHALDSLLLTSIKLARRGSKISIGAGSRGDRLT
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEE
VWVRTEGFGISPVALRSLFNPFRKGRNNRPGVEGGIALSLAKVGRIIEAHGGAVRVEGRE
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GAELLIKLALPLTTRSAARKGAAGAH
CHHHHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]