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The map label for this gene is ribE [H]

Identifier: 116623315

GI number: 116623315

Start: 5347363

End: 5348004

Strand: Direct

Name: ribE [H]

Synonym: Acid_4223

Alternate gene names: 116623315

Gene position: 5347363-5348004 (Clockwise)

Preceding gene: 116623314

Following gene: 116623317

Centisome position: 53.66

GC content: 63.86

Gene sequence:

>642_bases
ATGTTCACCGGGATTATTGAGGAACTCGGCGCTATCGAGGCCGTGCAGACGCGCGCCGCCGGAGCGCGCCTCACCATTCG
CTGCTCCGCCGTCCTGAGCGATGCCGTGGAAGGCGCGAGCATTGCGGTCAATGGCGTTTGCCTCACCGCAGTGGACCTTC
GCCCGGCCAGTTTTTCCGCCGACTTGGCGCCCGAGACTTTGCAGCGCAGCAACCTCGGCGACCTGCGCGCCGGCTCGCGC
GTCAACTTGGAGCGTCCCCTCTCCCCGACAGGCCGCTTGAACGGCCACATCGTGCAGGGCCACGTGGATGGCACCGGCGA
ATTTCTTTCGCTGGAAGCCCTCGGCGACGACAACTGGTGGCTCCGCATCCGGGTGCCCGCGGAACTCGATCCCTACCTGG
TCTTCAAAGGCTCGATCGCAATCGACGGCATCAGCCTCACCATCGCGTCACTGGAAAGCGACGTGCTCGCAGTGACCATC
ATCCCGCACACTTTCCGTGGCACCACTCTCGGCAGCTACCGCCCCGGCGCCCGGGTCAACCTGGAGTGCGACGTTCTCGC
CAAGCACGTGGAAAAACTGCTGAAGAAACTCGATCTGAAGGAAGCTCTCACGGTCGATAAGTTGCGCGCGCAGGGGTATT
GA

Upstream 100 bases:

>100_bases
GGCGGCATCGGCCGCCGCCGCCGTCAGGACGCCATTCAGCTGCACAACCTCAGCATCCACCAGATTCCGCCCGACGAATT
CGCAGTGGAAGGATACATCG

Downstream 100 bases:

>100_bases
AACCGTTTCTCGCAGAGCCGCAGAGAACCGCAGAGAAGAAAACTCCAGGTCTTGGTTTTCTTTGCGTTACTTTGCGGCTT
TGCGAGAAAAAGGGCCTTCC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MFTGIIEELGAIEAVQTRAAGARLTIRCSAVLSDAVEGASIAVNGVCLTAVDLRPASFSADLAPETLQRSNLGDLRAGSR
VNLERPLSPTGRLNGHIVQGHVDGTGEFLSLEALGDDNWWLRIRVPAELDPYLVFKGSIAIDGISLTIASLESDVLAVTI
IPHTFRGTTLGSYRPGARVNLECDVLAKHVEKLLKKLDLKEALTVDKLRAQGY

Sequences:

>Translated_213_residues
MFTGIIEELGAIEAVQTRAAGARLTIRCSAVLSDAVEGASIAVNGVCLTAVDLRPASFSADLAPETLQRSNLGDLRAGSR
VNLERPLSPTGRLNGHIVQGHVDGTGEFLSLEALGDDNWWLRIRVPAELDPYLVFKGSIAIDGISLTIASLESDVLAVTI
IPHTFRGTTLGSYRPGARVNLECDVLAKHVEKLLKKLDLKEALTVDKLRAQGY
>Mature_213_residues
MFTGIIEELGAIEAVQTRAAGARLTIRCSAVLSDAVEGASIAVNGVCLTAVDLRPASFSADLAPETLQRSNLGDLRAGSR
VNLERPLSPTGRLNGHIVQGHVDGTGEFLSLEALGDDNWWLRIRVPAELDPYLVFKGSIAIDGISLTIASLESDVLAVTI
IPHTFRGTTLGSYRPGARVNLECDVLAKHVEKLLKKLDLKEALTVDKLRAQGY

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=186, Percent_Identity=36.0215053763441, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319733, Length=208, Percent_Identity=36.0576923076923, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22797; Mature: 22797

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIIEELGAIEAVQTRAAGARLTIRCSAVLSDAVEGASIAVNGVCLTAVDLRPASFSA
CCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCEEEECCEEEEEEECCCCCCCC
DLAPETLQRSNLGDLRAGSRVNLERPLSPTGRLNGHIVQGHVDGTGEFLSLEALGDDNWW
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCEE
LRIRVPAELDPYLVFKGSIAIDGISLTIASLESDVLAVTIIPHTFRGTTLGSYRPGARVN
EEEEECCCCCCEEEEECCEEECCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCEEE
LECDVLAKHVEKLLKKLDLKEALTVDKLRAQGY
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFTGIIEELGAIEAVQTRAAGARLTIRCSAVLSDAVEGASIAVNGVCLTAVDLRPASFSA
CCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCCEEEECCEEEEEEECCCCCCCC
DLAPETLQRSNLGDLRAGSRVNLERPLSPTGRLNGHIVQGHVDGTGEFLSLEALGDDNWW
CCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCEE
LRIRVPAELDPYLVFKGSIAIDGISLTIASLESDVLAVTIIPHTFRGTTLGSYRPGARVN
EEEEECCCCCCEEEEECCEEECCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCEEE
LECDVLAKHVEKLLKKLDLKEALTVDKLRAQGY
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]