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The map label for this gene is blaB5 [H]

Identifier: 116623264

GI number: 116623264

Start: 5288464

End: 5289345

Strand: Reverse

Name: blaB5 [H]

Synonym: Acid_4171

Alternate gene names: 116623264

Gene position: 5289345-5288464 (Counterclockwise)

Preceding gene: 116623267

Following gene: 116623263

Centisome position: 53.08

GC content: 59.86

Gene sequence:

>882_bases
ATGACCGCGATGAAGCAGACGCTCAAAGTACGTGCTTGCGGCGCAGCCTTATTCTTCGCCTGTGTGCTCGCGGCCTACAC
CTTCCAGACCACACCTCCTCCCGAGTTGACCATCGAGAAGGTCCACGACGACCTGTATATGATCGTGGGCGATGGCGGGA
ATGTCGCCGTCTACATCACTAATGAGGGCCTCATCCTGGTAGACGACAAGTACGACCAGGACCACGCCCAGATCATGGCC
CGGATTCGCAGCGTCACCACTCAGCCCGTGCGCTACATCCTCAGCACGCACTATCATGCCGACCACAGCGGCGGCAACAC
ACAGTTCACCAAAGAGGCCGAGATCATCTCCACCCGCAATGCTCACGACGGCATCGTGAAGCACCTGCAATCCAACGCGC
CGGATAACATGGTTCCGGCCCGCGTGACCTTTACCGACGAAACCTCCCTCTTCCTCGGTGGCAAAGAAGTGCGCGCCAGG
TATTTCGGGCGGGCTCACACCAACGGCGACGCGTTCGTCTATTTCCCCGCGCATCGCCTGGTGCACTCCGGCGATACGTT
CGCCTCCGCCCCGACCGCTACGCCTCTCATCGATTATCCGGGCGGAGCCAGCCTCATCGAGTGGACCGGCACCCTCGACG
CCGTGATGAAGGCATGGGATTTCGATCAAGTTATACCCGGCCACGGTCCATTGAGCAACAAAGCCGGCATGAAAGCCTAC
CGCGATCGTGTGGAAGTGTTGCGGAGCAAGGCGCAGAGCCTGGTCCGCTCCGGCAAGTCGCAGGCCGACCTGGCGAAATT
CATGGAAACCGAATATAAGTGGGCGCCCGACAGCCTGTTTCAAAAGTGGAGCGTCCCAGGCATGATGACGGAGTTGAAGT
AG

Upstream 100 bases:

>100_bases
TTCGAAGAGATCGGAGTCGAGAAGTTGCAAGGCTGGCATTCTTACCAGGATGGTGGGTCCGGTGCGCGGGCGCAAGTAGC
GGCCTCCTGGCCGTGATACG

Downstream 100 bases:

>100_bases
CTTTGCCTACCCTCCGAAACCGGTTGACCTCCGTTGCGGCCCTTCTGCTATCCGCAACGGCCATCCCGGCCCAAACCGCA
GGTCCAACCCAGCCGGCGGC

Product: beta-lactamase domain-containing protein

Products: NA

Alternate protein names: CHbetaL-5; Class B carbapenemase BlaB-5 [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTAMKQTLKVRACGAALFFACVLAAYTFQTTPPPELTIEKVHDDLYMIVGDGGNVAVYITNEGLILVDDKYDQDHAQIMA
RIRSVTTQPVRYILSTHYHADHSGGNTQFTKEAEIISTRNAHDGIVKHLQSNAPDNMVPARVTFTDETSLFLGGKEVRAR
YFGRAHTNGDAFVYFPAHRLVHSGDTFASAPTATPLIDYPGGASLIEWTGTLDAVMKAWDFDQVIPGHGPLSNKAGMKAY
RDRVEVLRSKAQSLVRSGKSQADLAKFMETEYKWAPDSLFQKWSVPGMMTELK

Sequences:

>Translated_293_residues
MTAMKQTLKVRACGAALFFACVLAAYTFQTTPPPELTIEKVHDDLYMIVGDGGNVAVYITNEGLILVDDKYDQDHAQIMA
RIRSVTTQPVRYILSTHYHADHSGGNTQFTKEAEIISTRNAHDGIVKHLQSNAPDNMVPARVTFTDETSLFLGGKEVRAR
YFGRAHTNGDAFVYFPAHRLVHSGDTFASAPTATPLIDYPGGASLIEWTGTLDAVMKAWDFDQVIPGHGPLSNKAGMKAY
RDRVEVLRSKAQSLVRSGKSQADLAKFMETEYKWAPDSLFQKWSVPGMMTELK
>Mature_292_residues
TAMKQTLKVRACGAALFFACVLAAYTFQTTPPPELTIEKVHDDLYMIVGDGGNVAVYITNEGLILVDDKYDQDHAQIMAR
IRSVTTQPVRYILSTHYHADHSGGNTQFTKEAEIISTRNAHDGIVKHLQSNAPDNMVPARVTFTDETSLFLGGKEVRARY
FGRAHTNGDAFVYFPAHRLVHSGDTFASAPTATPLIDYPGGASLIEWTGTLDAVMKAWDFDQVIPGHGPLSNKAGMKAYR
DRVEVLRSKAQSLVRSGKSQADLAKFMETEYKWAPDSLFQKWSVPGMMTELK

Specific function: Hydrolyzes penicillins, cephalosporins (including cefoxitin), carbapenems and 6-beta-iodopenicillanate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-B beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001018
- InterPro:   IPR001279 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 32419; Mature: 32288

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAMKQTLKVRACGAALFFACVLAAYTFQTTPPPELTIEKVHDDLYMIVGDGGNVAVYIT
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCEEEEEE
NEGLILVDDKYDQDHAQIMARIRSVTTQPVRYILSTHYHADHSGGNTQFTKEAEIISTRN
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHCCCC
AHDGIVKHLQSNAPDNMVPARVTFTDETSLFLGGKEVRARYFGRAHTNGDAFVYFPAHRL
CHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHCCCCCCCCEEEEECHHHH
VHSGDTFASAPTATPLIDYPGGASLIEWTGTLDAVMKAWDFDQVIPGHGPLSNKAGMKAY
EECCCCCCCCCCCCCEEECCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
RDRVEVLRSKAQSLVRSGKSQADLAKFMETEYKWAPDSLFQKWSVPGMMTELK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
TAMKQTLKVRACGAALFFACVLAAYTFQTTPPPELTIEKVHDDLYMIVGDGGNVAVYIT
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCEEEEEE
NEGLILVDDKYDQDHAQIMARIRSVTTQPVRYILSTHYHADHSGGNTQFTKEAEIISTRN
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHCCCC
AHDGIVKHLQSNAPDNMVPARVTFTDETSLFLGGKEVRARYFGRAHTNGDAFVYFPAHRL
CHHHHHHHHHCCCCCCCCCEEEEECCCCEEEECCHHHHHHHHCCCCCCCCEEEEECHHHH
VHSGDTFASAPTATPLIDYPGGASLIEWTGTLDAVMKAWDFDQVIPGHGPLSNKAGMKAY
EECCCCCCCCCCCCCEEECCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
RDRVEVLRSKAQSLVRSGKSQADLAKFMETEYKWAPDSLFQKWSVPGMMTELK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10858348 [H]