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The map label for this gene is gspE [C]

Identifier: 116623253

GI number: 116623253

Start: 5276686

End: 5278050

Strand: Reverse

Name: gspE [C]

Synonym: Acid_4160

Alternate gene names: 116623253

Gene position: 5278050-5276686 (Counterclockwise)

Preceding gene: 116623254

Following gene: 116623252

Centisome position: 52.96

GC content: 64.1

Gene sequence:

>1365_bases
ATGACGGCCCCGACAGTGGAATCGCGCGTGGAATCCAACTGGGAGCAGCGTCTGTTGCAGAACGCGGCGAGCCCGGCGGC
GGTCAAGCCGGAGTATCAGGAGCTGAAGTTCACGCTGCACCGCAAGCTGCTCGAGCGGATCAACCTGGAGGCGCTCTCCG
GTATAGACGACGACCGGATCCACGCCGAGGTGCGGCAGGCGGTGCTCTCGATGGTGGAGGAAGAGCCCAATCTGCTGACC
GCGGCGGAGAAGCAGCGCATCAGCCACGAGGTGCTGCACGAGGTCTTCGGACTGGGGCCGCTGGAGCCTTTGCTGCAGGA
CGCGACCATCTCCGACATTCTGGTGAACGGGCATAAGCAGGTGTATGTGGAGCGCAAGGGCCTGCTCGAATTGACCAGCG
TGCGGTTTCACGATGAAGGCCACCTCCTGCGGATTATCGACAAGATCGTGTCGCAGGTGGGGCGGCGCGTGGATGAATCG
AATCCGATGGTGGACGCGCGCTTGAGCGACGGCTCGCGCGTGAATGCGATTATCCCGCCGCTGGCGGTGGATGGAGCGCT
GCTCTCGATCCGACGCTTCGGCACCGACAAGCTGCTGCCCGAGGACCTGGTGGCGAAGGGCGCGATGACGCGCGGCATGA
TGCTGCTGCTGGAGGCCGCGGTCAAGGCCAAGCTGAACATCATCGTGTCTGGGGGGACCGGCGCGGGCAAGACCACGCTG
TTGAACGCGCTCTCCTTCTTCATTTCGCCGAAGGAGCGGATCCTGACCATTGAGGACGCGGCCGAATTGCAGTTGAAGCA
ACCGCACGTGGTGCGGCTGGAGACGCGCCCGGACAACCTGGAGGGACACGGGGCGGTGCGGCAGCGCCAGCTGCTGATCA
ACAGCCTGCGCATGCGGCCCGACCGGATCATCGTGGGCGAGGTGCGCGGCGAAGAGGCGCTGGACATGCTGCAGGCGATG
AACACGGGCCACGACGGCTCGCTGACCACGGTACACGCCAATACGCCCCGCGATGCGGTATCGCGCCTGGAGGTGATGGT
GACGCTGGCTAATGCCAACATGCATCTGGCGGCGATCCGGCAGCAGATCACGTCGGCGGTGCAGATTCTGGTGCAGTGTT
CGCGCTTGAGCGACGGATCGCGGCGCGTTACCAGCATCACGGAGGTCACCGGCATGGAGGGCGAGATCGTCACCCTGCAG
GATATTTTCGTTTTTGAGAAGCGCGGCCTGGCGCGCGACGGCAGCGTGCTGGGCAGCTTTTGTGCCACCGGGATTATGCC
CAAGTTCAACGAAAAGCTGGTGGCATCGGGGATGCGGCTGCCCTCGGAGATCTTCAACGAACGAGTGCAAGCCGGAGGTT
TCTGA

Upstream 100 bases:

>100_bases
TCGCGGCCAGGGTCGCGGTAGCGCCCGATCCCGAACAGATGGTCCTCCTGATTGGCGCGGTCAGGAAGGTCCAGGTGTTC
GCGCCGGAACCGGGGACGAG

Downstream 100 bases:

>100_bases
TGCCGGTACTCTTCCTGGTAGCGGCGGCGACCGTGGTTCTCACCTACCGGCTGCTGTGGATGGGCCTGGAGCGGCGCGAT
GCGGCCCTGGCGGTACGGCG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTAPTVESRVESNWEQRLLQNAASPAAVKPEYQELKFTLHRKLLERINLEALSGIDDDRIHAEVRQAVLSMVEEEPNLLT
AAEKQRISHEVLHEVFGLGPLEPLLQDATISDILVNGHKQVYVERKGLLELTSVRFHDEGHLLRIIDKIVSQVGRRVDES
NPMVDARLSDGSRVNAIIPPLAVDGALLSIRRFGTDKLLPEDLVAKGAMTRGMMLLLEAAVKAKLNIIVSGGTGAGKTTL
LNALSFFISPKERILTIEDAAELQLKQPHVVRLETRPDNLEGHGAVRQRQLLINSLRMRPDRIIVGEVRGEEALDMLQAM
NTGHDGSLTTVHANTPRDAVSRLEVMVTLANANMHLAAIRQQITSAVQILVQCSRLSDGSRRVTSITEVTGMEGEIVTLQ
DIFVFEKRGLARDGSVLGSFCATGIMPKFNEKLVASGMRLPSEIFNERVQAGGF

Sequences:

>Translated_454_residues
MTAPTVESRVESNWEQRLLQNAASPAAVKPEYQELKFTLHRKLLERINLEALSGIDDDRIHAEVRQAVLSMVEEEPNLLT
AAEKQRISHEVLHEVFGLGPLEPLLQDATISDILVNGHKQVYVERKGLLELTSVRFHDEGHLLRIIDKIVSQVGRRVDES
NPMVDARLSDGSRVNAIIPPLAVDGALLSIRRFGTDKLLPEDLVAKGAMTRGMMLLLEAAVKAKLNIIVSGGTGAGKTTL
LNALSFFISPKERILTIEDAAELQLKQPHVVRLETRPDNLEGHGAVRQRQLLINSLRMRPDRIIVGEVRGEEALDMLQAM
NTGHDGSLTTVHANTPRDAVSRLEVMVTLANANMHLAAIRQQITSAVQILVQCSRLSDGSRRVTSITEVTGMEGEIVTLQ
DIFVFEKRGLARDGSVLGSFCATGIMPKFNEKLVASGMRLPSEIFNERVQAGGF
>Mature_453_residues
TAPTVESRVESNWEQRLLQNAASPAAVKPEYQELKFTLHRKLLERINLEALSGIDDDRIHAEVRQAVLSMVEEEPNLLTA
AEKQRISHEVLHEVFGLGPLEPLLQDATISDILVNGHKQVYVERKGLLELTSVRFHDEGHLLRIIDKIVSQVGRRVDESN
PMVDARLSDGSRVNAIIPPLAVDGALLSIRRFGTDKLLPEDLVAKGAMTRGMMLLLEAAVKAKLNIIVSGGTGAGKTTLL
NALSFFISPKERILTIEDAAELQLKQPHVVRLETRPDNLEGHGAVRQRQLLINSLRMRPDRIIVGEVRGEEALDMLQAMN
TGHDGSLTTVHANTPRDAVSRLEVMVTLANANMHLAAIRQQITSAVQILVQCSRLSDGSRRVTSITEVTGMEGEIVTLQD
IFVFEKRGLARDGSVLGSFCATGIMPKFNEKLVASGMRLPSEIFNERVQAGGF

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=122, Percent_Identity=39.344262295082, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 49998; Mature: 49867

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPTVESRVESNWEQRLLQNAASPAAVKPEYQELKFTLHRKLLERINLEALSGIDDDRI
CCCCCHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCHHHH
HAEVRQAVLSMVEEEPNLLTAAEKQRISHEVLHEVFGLGPLEPLLQDATISDILVNGHKQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCCE
VYVERKGLLELTSVRFHDEGHLLRIIDKIVSQVGRRVDESNPMVDARLSDGSRVNAIIPP
EEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCC
LAVDGALLSIRRFGTDKLLPEDLVAKGAMTRGMMLLLEAAVKAKLNIIVSGGTGAGKTTL
HHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHH
LNALSFFISPKERILTIEDAAELQLKQPHVVRLETRPDNLEGHGAVRQRQLLINSLRMRP
HHHHHHHHCCHHHEEEEECHHHHEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCC
DRIIVGEVRGEEALDMLQAMNTGHDGSLTTVHANTPRDAVSRLEVMVTLANANMHLAAIR
CEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHEEEEECCCHHHHHHH
QQITSAVQILVQCSRLSDGSRRVTSITEVTGMEGEIVTLQDIFVFEKRGLARDGSVLGSF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHH
CATGIMPKFNEKLVASGMRLPSEIFNERVQAGGF
HHHCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TAPTVESRVESNWEQRLLQNAASPAAVKPEYQELKFTLHRKLLERINLEALSGIDDDRI
CCCCHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHCCCCHHHH
HAEVRQAVLSMVEEEPNLLTAAEKQRISHEVLHEVFGLGPLEPLLQDATISDILVNGHKQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHCCCCE
VYVERKGLLELTSVRFHDEGHLLRIIDKIVSQVGRRVDESNPMVDARLSDGSRVNAIIPP
EEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCC
LAVDGALLSIRRFGTDKLLPEDLVAKGAMTRGMMLLLEAAVKAKLNIIVSGGTGAGKTTL
HHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHH
LNALSFFISPKERILTIEDAAELQLKQPHVVRLETRPDNLEGHGAVRQRQLLINSLRMRP
HHHHHHHHCCHHHEEEEECHHHHEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCC
DRIIVGEVRGEEALDMLQAMNTGHDGSLTTVHANTPRDAVSRLEVMVTLANANMHLAAIR
CEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHEEEEECCCHHHHHHH
QQITSAVQILVQCSRLSDGSRRVTSITEVTGMEGEIVTLQDIFVFEKRGLARDGSVLGSF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCHHHHHHH
CATGIMPKFNEKLVASGMRLPSEIFNERVQAGGF
HHHCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]