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The map label for this gene is ada [H]

Identifier: 116623105

GI number: 116623105

Start: 5080345

End: 5081412

Strand: Reverse

Name: ada [H]

Synonym: Acid_4009

Alternate gene names: 116623105

Gene position: 5081412-5080345 (Counterclockwise)

Preceding gene: 116623111

Following gene: 116623104

Centisome position: 50.99

GC content: 68.54

Gene sequence:

>1068_bases
ATGGAAGTAGCAATGGACGAGACAGTAAAAACCGACGCGCAGCGCTGGGCGGCCATCCTGCGCCGCGACCCGGCGGCCGA
CGGCGAGTTCTTCTATTCGGTGAAGACCACCGGCGTATATTGCCGGCCTTCCTGCGCCTCGCGGCTCGCGCTGCGCAAGA
ACGTCGTATTCCACGCGACCACGGAGGCGGCCGAGCGCGCCGGCTTCCGTCCCTGCAAGCGCTGCAAGCCCGATCAGGCG
GCGCTCGCGGAACAGCATGCCGCCGCCATCGCCGCGGCCTGCCGAACAATTGAGGTCTCGGAGACCACCCCATCGCTGGA
TGAACTGGCGGCGGTCGCAGGCATGAGCCGCTTCTATTTTCACCGGACCTTCAAAGCCATCACGGGCGTGACGCCCAAAG
CCTACGCCTCCGCGCGGCGCGCCGAGCGGGTACGCACCGAACTTGCCGCCAGCCCCACCGTCACGCAGGCTATCTACGAC
GCGGGCTTCAACTCCAGCGGCCGCTTCTACGAGCAGTCAACGGACATCCTCGGTATGACGCCCGGTGATTTCCGCGCCGG
CGGCTCCCGTGCCGCTATCCGCTTCGCCGTGGCCGAGTGCTCGCTCGGCTCCATCCTGGTCGCCGCAACCGAGAAGGGTG
TGTGTGCCATCCTGATGGGCGACGATCCCGAAACACTGGTGCGCGACCTTCAGGATCGCTTCCCGCGCGCCGAACTCACC
GGCGGCGATCGTGAGTTCGAGGCCACCGTCGCCAAGGTGCTGACGTTCGTCGAGGCGCCCGGCCTCGGCCTCGATCTGCC
GCTCGACGTGCGCGGGACCGCCTTCCAGCAGCGCGTCTGGCAGGCCTTGCGCGCCATTCCGGCCGGCGCCACCGCCAGCT
ACGCGGAGATCGCAGCCCGCATCGGCGCTCCCAAATCGGTACGTGCCGTGGCGCAGGCCTGCGCGGCGAACTCGCTCGCC
ATCGCCATCCCTTGCCATCGCGTCGTCCGGCATGATGGCGGACTTTCCGGCTACCGCTGGGGAGTGGAGCGCAAACGCGC
CCTGCTCACCCGGGAGGGTGCGCGCTGA

Upstream 100 bases:

>100_bases
GGCGGGCGAGCATGCCGTCAGTGTGTCACAGAATTTGAAAGCAAGCGGCGGAGTGTTTCCCATCGGCGCCTCTCCCAAGA
TGGAGTGGAGGAGAGTCATC

Downstream 100 bases:

>100_bases
TGGATTGGGAACGCATCTCCGCCGATCTCGACCAATACGGTTGCGCCGTTGTCAACGCCGTGCTCTCCGGGAAAGAGTGT
CGCCGGCTGGCGTCCCTTTA

Product: DNA-O6-methylguanine--protein-cysteine S-methyltransferase/transcriptional regulator Ada

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MEVAMDETVKTDAQRWAAILRRDPAADGEFFYSVKTTGVYCRPSCASRLALRKNVVFHATTEAAERAGFRPCKRCKPDQA
ALAEQHAAAIAAACRTIEVSETTPSLDELAAVAGMSRFYFHRTFKAITGVTPKAYASARRAERVRTELAASPTVTQAIYD
AGFNSSGRFYEQSTDILGMTPGDFRAGGSRAAIRFAVAECSLGSILVAATEKGVCAILMGDDPETLVRDLQDRFPRAELT
GGDREFEATVAKVLTFVEAPGLGLDLPLDVRGTAFQQRVWQALRAIPAGATASYAEIAARIGAPKSVRAVAQACAANSLA
IAIPCHRVVRHDGGLSGYRWGVERKRALLTREGAR

Sequences:

>Translated_355_residues
MEVAMDETVKTDAQRWAAILRRDPAADGEFFYSVKTTGVYCRPSCASRLALRKNVVFHATTEAAERAGFRPCKRCKPDQA
ALAEQHAAAIAAACRTIEVSETTPSLDELAAVAGMSRFYFHRTFKAITGVTPKAYASARRAERVRTELAASPTVTQAIYD
AGFNSSGRFYEQSTDILGMTPGDFRAGGSRAAIRFAVAECSLGSILVAATEKGVCAILMGDDPETLVRDLQDRFPRAELT
GGDREFEATVAKVLTFVEAPGLGLDLPLDVRGTAFQQRVWQALRAIPAGATASYAEIAARIGAPKSVRAVAQACAANSLA
IAIPCHRVVRHDGGLSGYRWGVERKRALLTREGAR
>Mature_355_residues
MEVAMDETVKTDAQRWAAILRRDPAADGEFFYSVKTTGVYCRPSCASRLALRKNVVFHATTEAAERAGFRPCKRCKPDQA
ALAEQHAAAIAAACRTIEVSETTPSLDELAAVAGMSRFYFHRTFKAITGVTPKAYASARRAERVRTELAASPTVTQAIYD
AGFNSSGRFYEQSTDILGMTPGDFRAGGSRAAIRFAVAECSLGSILVAATEKGVCAILMGDDPETLVRDLQDRFPRAELT
GGDREFEATVAKVLTFVEAPGLGLDLPLDVRGTAFQQRVWQALRAIPAGATASYAEIAARIGAPKSVRAVAQACAANSLA
IAIPCHRVVRHDGGLSGYRWGVERKRALLTREGAR

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=162, Percent_Identity=30.8641975308642, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788542, Length=345, Percent_Identity=54.2028985507246, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=44.3181818181818, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=168, Percent_Identity=38.6904761904762, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI115533070, Length=133, Percent_Identity=41.3533834586466, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=43.2098765432099, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137554, Length=143, Percent_Identity=38.4615384615385, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 38176; Mature: 38176

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVAMDETVKTDAQRWAAILRRDPAADGEFFYSVKTTGVYCRPSCASRLALRKNVVFHAT
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEECCHHHHHHHHHHCEEEEEC
TEAAERAGFRPCKRCKPDQAALAEQHAAAIAAACRTIEVSETTPSLDELAAVAGMSRFYF
HHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
HRTFKAITGVTPKAYASARRAERVRTELAASPTVTQAIYDAGFNSSGRFYEQSTDILGMT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCCCEEECC
PGDFRAGGSRAAIRFAVAECSLGSILVAATEKGVCAILMGDDPETLVRDLQDRFPRAELT
CCCCCCCCCCCEEEEEEHHCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCCC
GGDREFEATVAKVLTFVEAPGLGLDLPLDVRGTAFQQRVWQALRAIPAGATASYAEIAAR
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IGAPKSVRAVAQACAANSLAIAIPCHRVVRHDGGLSGYRWGVERKRALLTREGAR
HCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MEVAMDETVKTDAQRWAAILRRDPAADGEFFYSVKTTGVYCRPSCASRLALRKNVVFHAT
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEECCHHHHHHHHHHCEEEEEC
TEAAERAGFRPCKRCKPDQAALAEQHAAAIAAACRTIEVSETTPSLDELAAVAGMSRFYF
HHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
HRTFKAITGVTPKAYASARRAERVRTELAASPTVTQAIYDAGFNSSGRFYEQSTDILGMT
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEECCCCEEECC
PGDFRAGGSRAAIRFAVAECSLGSILVAATEKGVCAILMGDDPETLVRDLQDRFPRAELT
CCCCCCCCCCCEEEEEEHHCCCCCEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCCCCC
GGDREFEATVAKVLTFVEAPGLGLDLPLDVRGTAFQQRVWQALRAIPAGATASYAEIAAR
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IGAPKSVRAVAQACAANSLAIAIPCHRVVRHDGGLSGYRWGVERKRALLTREGAR
HCCCHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]