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The map label for this gene is alkB [H]

Identifier: 116623103

GI number: 116623103

Start: 5079024

End: 5079668

Strand: Reverse

Name: alkB [H]

Synonym: Acid_4007

Alternate gene names: 116623103

Gene position: 5079668-5079024 (Counterclockwise)

Preceding gene: 116623104

Following gene: 116623101

Centisome position: 50.97

GC content: 66.82

Gene sequence:

>645_bases
ATGATGAACACTCTCTTCGATTCCGAACCCGGCGGCGCCGAACCAATCGGCGAGGGAGCGGTCGTGCTGCACGGCTTCGC
GCGCGAGGCCGCGTCCACGCTCCTCCACGATCTCGGCAAGATCGCCGAGGCCGCGCCCTTCCGCCACATGGTCACTCCCG
GCGGATATCGCATGTCGGTCGCGATGACCAATTGCGGCGACGTCGGCTGGGTCTCCGATGCGCGCGGCTACCGGTACGAT
CCCGTGGATCCGGCCACCGGACTGCCGTGGCCTGCGATGCCGGAGAGCTTCCGCAGTCTCGCGCTCGATGCGGCCGCAGC
CGCGGGTTATCCGGAATTCGATCCCGACGCCTGCCTCGTCAATCGCTACGAGCCCGGCGCCCGGCTCACTCTCCACCAGG
ACAAAGACGAGACCGATTACACCGCCCCCATAGTCTCCGTTTCCCTTGGACTCCCCGCCGTCTTTCAGTTCGGCGGCATG
ATCCGCAAGGTCCGCCCCCGCCGCATGCGCCTGGAAAGCGGCGACGTGGTCGTGTGGGGCGGTCCCACTCGTCTCGCCTA
TCACGGCGTTGCGCCTCTCAAACCGGGCGATCACCCGCTCACCGGCCCGTTCCGCATCAATCTCACCTTTCGCAAGGCCC
TCTAA

Upstream 100 bases:

>100_bases
AAGGCACGCGCGGTGCGTACCGGGTAAACCTGCGCCATGGCGTGAGCCGCCTGCGCTCCGGCACGCGCCACACGCTCGGC
ATCATTTTTCACGACGCTTT

Downstream 100 bases:

>100_bases
TTGATTGGCTCCCCGGGCGAGATTCCGTCCACCCGGTTGACCTTCGCTCCAGCCGGTAGCGGCACTGCACCTGAGAGCAA
GGGATCGTTGGTCCGCTTCA

Product: DNA-N1-methyladenine dioxygenase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MMNTLFDSEPGGAEPIGEGAVVLHGFAREAASTLLHDLGKIAEAAPFRHMVTPGGYRMSVAMTNCGDVGWVSDARGYRYD
PVDPATGLPWPAMPESFRSLALDAAAAAGYPEFDPDACLVNRYEPGARLTLHQDKDETDYTAPIVSVSLGLPAVFQFGGM
IRKVRPRRMRLESGDVVVWGGPTRLAYHGVAPLKPGDHPLTGPFRINLTFRKAL

Sequences:

>Translated_214_residues
MMNTLFDSEPGGAEPIGEGAVVLHGFAREAASTLLHDLGKIAEAAPFRHMVTPGGYRMSVAMTNCGDVGWVSDARGYRYD
PVDPATGLPWPAMPESFRSLALDAAAAAGYPEFDPDACLVNRYEPGARLTLHQDKDETDYTAPIVSVSLGLPAVFQFGGM
IRKVRPRRMRLESGDVVVWGGPTRLAYHGVAPLKPGDHPLTGPFRINLTFRKAL
>Mature_214_residues
MMNTLFDSEPGGAEPIGEGAVVLHGFAREAASTLLHDLGKIAEAAPFRHMVTPGGYRMSVAMTNCGDVGWVSDARGYRYD
PVDPATGLPWPAMPESFRSLALDAAAAAGYPEFDPDACLVNRYEPGARLTLHQDKDETDYTAPIVSVSLGLPAVFQFGGM
IRKVRPRRMRLESGDVVVWGGPTRLAYHGVAPLKPGDHPLTGPFRINLTFRKAL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=127, Percent_Identity=31.496062992126, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1788541, Length=213, Percent_Identity=61.5023474178404, Blast_Score=273, Evalue=5e-75,
Organism=Drosophila melanogaster, GI45555401, Length=98, Percent_Identity=40.8163265306122, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23107; Mature: 23107

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNTLFDSEPGGAEPIGEGAVVLHGFAREAASTLLHDLGKIAEAAPFRHMVTPGGYRMSV
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEE
AMTNCGDVGWVSDARGYRYDPVDPATGLPWPAMPESFRSLALDAAAAAGYPEFDPDACLV
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
NRYEPGARLTLHQDKDETDYTAPIVSVSLGLPAVFQFGGMIRKVRPRRMRLESGDVVVWG
ECCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHEEECCCCEEEEC
GPTRLAYHGVAPLKPGDHPLTGPFRINLTFRKAL
CCCEEEECCCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MMNTLFDSEPGGAEPIGEGAVVLHGFAREAASTLLHDLGKIAEAAPFRHMVTPGGYRMSV
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEE
AMTNCGDVGWVSDARGYRYDPVDPATGLPWPAMPESFRSLALDAAAAAGYPEFDPDACLV
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEE
NRYEPGARLTLHQDKDETDYTAPIVSVSLGLPAVFQFGGMIRKVRPRRMRLESGDVVVWG
ECCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHEEECCCCEEEEC
GPTRLAYHGVAPLKPGDHPLTGPFRINLTFRKAL
CCCEEEECCCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]