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The map label for this gene is afr [H]

Identifier: 116623046

GI number: 116623046

Start: 5000234

End: 5001427

Strand: Reverse

Name: afr [H]

Synonym: Acid_3950

Alternate gene names: 116623046

Gene position: 5001427-5000234 (Counterclockwise)

Preceding gene: 116623050

Following gene: 116623045

Centisome position: 50.19

GC content: 64.24

Gene sequence:

>1194_bases
ATGGAAAATCCCAATCCTCATCGCCGCCAGTTTGTGCGCGCCGCGCTGGTCACCGCCGCGTCCTACAGCAGAATCCTGGG
CGCCAACGATCGCATTCGTATCGGCGCGATCGGCACCGGCGGACGCTGCCAGTACCTGCTGAGCCAATTAAAGATGCTCG
AAGACAACGAAATTGTGGCGTGCTGCGACGTCTACGAGCCGCGCCGCCTCGAAGCCAAGACGAAGTACGGCACCGCGGAC
GCCAGCGTTCACGTAGACTATCGCGAGATCCTCGACCGCAAGGATATCGACGGCGTCGTGGTGGGAACGCCCGATCACTG
GCACGTCCCCGTCACGATTGCCAGTGTGCGCGCAGGTAAGGACGTGTACTGCGAGAAGCCCGTCACGCACACCCTGGAAG
AGGCCGCGCCGCTGCTGGCTGCGGTGCGCGAGACCAACCGCGTCCTGCAGACCGGCACGCAGCAGCGCAGTTGGGAACAC
TTCGCCCAGGCGCGCGACGTCATCGCCGAGGGGCGTCTCGGCCAGGTCACGCTGATTCGTACCTACTGGTTCCAGAACCA
CATTCCCGCCATCGGGCGCGTGCCCAGCTTCGATACCACCAAACTGGATTGGAAGCGCTTCCTGGGCAGCGCGGCCGACC
GCCCGTTCGACCCCGATCAGTTCGGCAACTGGCGCTGGTATTGGGATTTCGGCGGCGGCGCCATGACCGATCTCTTTATT
CACTGGGTGGATGTGGCGCACTGGATCATGGGCGACGACAGCCCGTCGCGCGCCACGGCCAACGGGCTCAAAGCGGTGCT
GCTGCAGCGTCAGACCCCGGACACGATGAGCGCCGCCCTGGCTTATGGCAAGGTGATCGTGGAGTTCGATTGCGCGCTGC
TCGGCTACATCGAAGGTGGCGGCCTGATGATCCGCGGCACCAAGGCGGGCATGCGCCTGTGGCGCGGCGGGTTCGAGATC
TACGATGAGATCCCGCGCTACAGCGAGAACCCCAACCCATCGAATGCCGCCATGCAAGTCAAATCGAAACAGGACGGCAC
GCTACCGCACATGAAGAACTTTCTGGACTGTGTGCGATCTCGCAAGCAGCCCAATGCGCATGTGGAAATCGGAATTGCCG
CCGCGCGCGCCGGGCACGTCGCCAACCTCGCGATGCGCGGCAGCGGTGTGTGGAACGCCGGAGGCCGCGGATGA

Upstream 100 bases:

>100_bases
GCTGCGTCGGTTGAGCGTGTCGGACATGACGCTCCTTATTAGATCACCCTCAGATCACCCATGCGCGCGAGCTGGAAGCT
CGATATACTGCAAGCAATTT

Downstream 100 bases:

>100_bases
AACCGGGGTTCGGCCTCGACATGGCCTTCCTTCCCCGCTAACGCCATTGCTGCATACCCTGTCCCCGGGAAAGATCGCCG
GGAAGATGCTGCACACAGGC

Product: oxidoreductase domain-containing protein

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MENPNPHRRQFVRAALVTAASYSRILGANDRIRIGAIGTGGRCQYLLSQLKMLEDNEIVACCDVYEPRRLEAKTKYGTAD
ASVHVDYREILDRKDIDGVVVGTPDHWHVPVTIASVRAGKDVYCEKPVTHTLEEAAPLLAAVRETNRVLQTGTQQRSWEH
FAQARDVIAEGRLGQVTLIRTYWFQNHIPAIGRVPSFDTTKLDWKRFLGSAADRPFDPDQFGNWRWYWDFGGGAMTDLFI
HWVDVAHWIMGDDSPSRATANGLKAVLLQRQTPDTMSAALAYGKVIVEFDCALLGYIEGGGLMIRGTKAGMRLWRGGFEI
YDEIPRYSENPNPSNAAMQVKSKQDGTLPHMKNFLDCVRSRKQPNAHVEIGIAAARAGHVANLAMRGSGVWNAGGRG

Sequences:

>Translated_397_residues
MENPNPHRRQFVRAALVTAASYSRILGANDRIRIGAIGTGGRCQYLLSQLKMLEDNEIVACCDVYEPRRLEAKTKYGTAD
ASVHVDYREILDRKDIDGVVVGTPDHWHVPVTIASVRAGKDVYCEKPVTHTLEEAAPLLAAVRETNRVLQTGTQQRSWEH
FAQARDVIAEGRLGQVTLIRTYWFQNHIPAIGRVPSFDTTKLDWKRFLGSAADRPFDPDQFGNWRWYWDFGGGAMTDLFI
HWVDVAHWIMGDDSPSRATANGLKAVLLQRQTPDTMSAALAYGKVIVEFDCALLGYIEGGGLMIRGTKAGMRLWRGGFEI
YDEIPRYSENPNPSNAAMQVKSKQDGTLPHMKNFLDCVRSRKQPNAHVEIGIAAARAGHVANLAMRGSGVWNAGGRG
>Mature_397_residues
MENPNPHRRQFVRAALVTAASYSRILGANDRIRIGAIGTGGRCQYLLSQLKMLEDNEIVACCDVYEPRRLEAKTKYGTAD
ASVHVDYREILDRKDIDGVVVGTPDHWHVPVTIASVRAGKDVYCEKPVTHTLEEAAPLLAAVRETNRVLQTGTQQRSWEH
FAQARDVIAEGRLGQVTLIRTYWFQNHIPAIGRVPSFDTTKLDWKRFLGSAADRPFDPDQFGNWRWYWDFGGGAMTDLFI
HWVDVAHWIMGDDSPSRATANGLKAVLLQRQTPDTMSAALAYGKVIVEFDCALLGYIEGGGLMIRGTKAGMRLWRGGFEI
YDEIPRYSENPNPSNAAMQVKSKQDGTLPHMKNFLDCVRSRKQPNAHVEIGIAAARAGHVANLAMRGSGVWNAGGRG

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI87081947, Length=280, Percent_Identity=26.4285714285714, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 44175; Mature: 44175

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENPNPHRRQFVRAALVTAASYSRILGANDRIRIGAIGTGGRCQYLLSQLKMLEDNEIVA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEE
CCDVYEPRRLEAKTKYGTADASVHVDYREILDRKDIDGVVVGTPDHWHVPVTIASVRAGK
EECCCCCCCCCCCCCCCCCCCEEEECHHHHCCCCCCCEEEECCCCCEEEEEEEEEECCCC
DVYCEKPVTHTLEEAAPLLAAVRETNRVLQTGTQQRSWEHFAQARDVIAEGRLGQVTLIR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
TYWFQNHIPAIGRVPSFDTTKLDWKRFLGSAADRPFDPDQFGNWRWYWDFGGGAMTDLFI
EEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHH
HWVDVAHWIMGDDSPSRATANGLKAVLLQRQTPDTMSAALAYGKVIVEFDCALLGYIEGG
HHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHCEEEEEECCEEEEEEECC
GLMIRGTKAGMRLWRGGFEIYDEIPRYSENPNPSNAAMQVKSKQDGTLPHMKNFLDCVRS
CEEEEECHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
RKQPNAHVEIGIAAARAGHVANLAMRGSGVWNAGGRG
CCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MENPNPHRRQFVRAALVTAASYSRILGANDRIRIGAIGTGGRCQYLLSQLKMLEDNEIVA
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEE
CCDVYEPRRLEAKTKYGTADASVHVDYREILDRKDIDGVVVGTPDHWHVPVTIASVRAGK
EECCCCCCCCCCCCCCCCCCCEEEECHHHHCCCCCCCEEEECCCCCEEEEEEEEEECCCC
DVYCEKPVTHTLEEAAPLLAAVRETNRVLQTGTQQRSWEHFAQARDVIAEGRLGQVTLIR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
TYWFQNHIPAIGRVPSFDTTKLDWKRFLGSAADRPFDPDQFGNWRWYWDFGGGAMTDLFI
EEHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHH
HWVDVAHWIMGDDSPSRATANGLKAVLLQRQTPDTMSAALAYGKVIVEFDCALLGYIEGG
HHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHCEEEEEECCEEEEEEECC
GLMIRGTKAGMRLWRGGFEIYDEIPRYSENPNPSNAAMQVKSKQDGTLPHMKNFLDCVRS
CEEEEECHHHHHHHHCCHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
RKQPNAHVEIGIAAARAGHVANLAMRGSGVWNAGGRG
CCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA