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The map label for this gene is yvaG [H]

Identifier: 116622984

GI number: 116622984

Start: 4918317

End: 4919111

Strand: Reverse

Name: yvaG [H]

Synonym: Acid_3887

Alternate gene names: 116622984

Gene position: 4919111-4918317 (Counterclockwise)

Preceding gene: 116622987

Following gene: 116622983

Centisome position: 49.36

GC content: 63.9

Gene sequence:

>795_bases
ATGGATCTCGAACTCAATGGGAAATTAGCCCTCGTGACAGGCTCGACAGCCGGCATCGGCCTGGCGATTGCGAGCGGGTT
GGCGAGGGAAGGCGCGCGTGTGATTGTGAACGGGCGCACGCAGGCGCGTGTGGACGAAGCGCGGCGGCGTATTCTGGAAC
AACAAGCCGTGGGCGAGGTCTTGACGTTCGCGGGCGATCTGTCGGAGGCGCAGACGGCGGCCGCTCTGGTGCGGGCCTAT
CCCGAAGTGGACATTTTGGTGAACAACCTCGGCAGGTACGCAGTGAAGCCCTTCGAGGAGCTGACCGACGCGGACTGGCT
GGGGATCATCGAGACCAACTTTATGAGCGGCGTGCGGCTGAGCCGGCATTATCTGCCGCTGATGAAGCGCGAAGGCTGGG
GCCGCGTGATCTTCATTTCGAGCGAATCGGCGGTGAACATTCCGACCGAGATGATTCATTACGGCGTGACCAAGACGATG
CAGGTGGCGCTGGCGCGCGGGTTGGCGGAGACCACGGCGGGCACGGGGGTGACGGTGAATTCGATCCTGGCAGGGCCGAC
GCGGTCGGAAGGCGTCGGCGGCTTCCTGGAACAGATGGCGAAGCAGCAGGGGACCACGGTGGCAGCGGTGGAAAAGGAGT
TCTTCCAGACGGCGCGCGGGTCTTCGCTGCTGAAACGGTTTGCGACGTCGGAAGAAGTGGCGGCGATGGTGGTGTTTGTG
GCGAGTCCGCTCTCGTCGGCCACCAATGGGGCAGCCTTGCGGGTTGAGGGCGGGGTAGTGCGGTCGATCCTGTAA

Upstream 100 bases:

>100_bases
CCGGAGGGCGCTGAGTTTGTCGAGCTGTTCCTCAATGCGTCGGTTGGCCATCCATAATTTCCCGTGACTCCCAGGATAGC
CTCGCTGCATCCAAGGAGTC

Downstream 100 bases:

>100_bases
GCTAATTCAGAGAGGGTAACGATTCATGAAACGAACAGCGAATGCGCACTGGCGCGGTGATTTGAAGACGGGGAAAGGGG
ACATCTCGACGGCCAGCGGT

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MDLELNGKLALVTGSTAGIGLAIASGLAREGARVIVNGRTQARVDEARRRILEQQAVGEVLTFAGDLSEAQTAAALVRAY
PEVDILVNNLGRYAVKPFEELTDADWLGIIETNFMSGVRLSRHYLPLMKREGWGRVIFISSESAVNIPTEMIHYGVTKTM
QVALARGLAETTAGTGVTVNSILAGPTRSEGVGGFLEQMAKQQGTTVAAVEKEFFQTARGSSLLKRFATSEEVAAMVVFV
ASPLSSATNGAALRVEGGVVRSIL

Sequences:

>Translated_264_residues
MDLELNGKLALVTGSTAGIGLAIASGLAREGARVIVNGRTQARVDEARRRILEQQAVGEVLTFAGDLSEAQTAAALVRAY
PEVDILVNNLGRYAVKPFEELTDADWLGIIETNFMSGVRLSRHYLPLMKREGWGRVIFISSESAVNIPTEMIHYGVTKTM
QVALARGLAETTAGTGVTVNSILAGPTRSEGVGGFLEQMAKQQGTTVAAVEKEFFQTARGSSLLKRFATSEEVAAMVVFV
ASPLSSATNGAALRVEGGVVRSIL
>Mature_264_residues
MDLELNGKLALVTGSTAGIGLAIASGLAREGARVIVNGRTQARVDEARRRILEQQAVGEVLTFAGDLSEAQTAAALVRAY
PEVDILVNNLGRYAVKPFEELTDADWLGIIETNFMSGVRLSRHYLPLMKREGWGRVIFISSESAVNIPTEMIHYGVTKTM
QVALARGLAETTAGTGVTVNSILAGPTRSEGVGGFLEQMAKQQGTTVAAVEKEFFQTARGSSLLKRFATSEEVAAMVVFV
ASPLSSATNGAALRVEGGVVRSIL

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI5031737, Length=264, Percent_Identity=31.0606060606061, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI32483357, Length=265, Percent_Identity=29.0566037735849, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI19923817, Length=262, Percent_Identity=25.5725190839695, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI126723191, Length=210, Percent_Identity=31.9047619047619, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=33.502538071066, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI7705855, Length=184, Percent_Identity=31.5217391304348, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI7657478, Length=257, Percent_Identity=29.1828793774319, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787905, Length=267, Percent_Identity=29.2134831460674, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787335, Length=258, Percent_Identity=30.6201550387597, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790717, Length=272, Percent_Identity=27.5735294117647, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082100, Length=264, Percent_Identity=31.0606060606061, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789057, Length=259, Percent_Identity=27.7992277992278, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082160, Length=270, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1789378, Length=262, Percent_Identity=28.2442748091603, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787820, Length=178, Percent_Identity=29.7752808988764, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789208, Length=274, Percent_Identity=25.1824817518248, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786812, Length=259, Percent_Identity=25.4826254826255, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI2367365, Length=256, Percent_Identity=27.34375, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=260, Percent_Identity=33.4615384615385, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560676, Length=261, Percent_Identity=29.1187739463602, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=28.6259541984733, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193204405, Length=272, Percent_Identity=30.8823529411765, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531453, Length=274, Percent_Identity=30.2919708029197, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=258, Percent_Identity=29.0697674418605, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17568967, Length=195, Percent_Identity=24.6153846153846, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=272, Percent_Identity=30.8823529411765, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=272, Percent_Identity=25.3676470588235, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=259, Percent_Identity=26.2548262548263, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=266, Percent_Identity=27.4436090225564, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=271, Percent_Identity=28.7822878228782, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=263, Percent_Identity=26.615969581749, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=190, Percent_Identity=23.6842105263158, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355319, Length=258, Percent_Identity=28.6821705426357, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI45553409, Length=275, Percent_Identity=28, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24647948, Length=275, Percent_Identity=28, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI23397609, Length=263, Percent_Identity=30.7984790874525, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647946, Length=276, Percent_Identity=27.8985507246377, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644339, Length=263, Percent_Identity=26.9961977186312, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI18859837, Length=194, Percent_Identity=29.3814432989691, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24643142, Length=258, Percent_Identity=29.4573643410853, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21357041, Length=256, Percent_Identity=26.5625, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21358495, Length=196, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24639444, Length=263, Percent_Identity=28.5171102661597, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 28026; Mature: 28026

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLELNGKLALVTGSTAGIGLAIASGLAREGARVIVNGRTQARVDEARRRILEQQAVGEV
CCEEECCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
LTFAGDLSEAQTAAALVRAYPEVDILVNNLGRYAVKPFEELTDADWLGIIETNFMSGVRL
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCHHHHCCCCEEEEEHHHHHHHHHH
SRHYLPLMKREGWGRVIFISSESAVNIPTEMIHYGVTKTMQVALARGLAETTAGTGVTVN
HHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SILAGPTRSEGVGGFLEQMAKQQGTTVAAVEKEFFQTARGSSLLKRFATSEEVAAMVVFV
HEECCCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHH
ASPLSSATNGAALRVEGGVVRSIL
HCCCCCCCCCCEEEECCCHHHHCC
>Mature Secondary Structure
MDLELNGKLALVTGSTAGIGLAIASGLAREGARVIVNGRTQARVDEARRRILEQQAVGEV
CCEEECCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
LTFAGDLSEAQTAAALVRAYPEVDILVNNLGRYAVKPFEELTDADWLGIIETNFMSGVRL
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHCCCHHHHCCCCEEEEEHHHHHHHHHH
SRHYLPLMKREGWGRVIFISSESAVNIPTEMIHYGVTKTMQVALARGLAETTAGTGVTVN
HHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SILAGPTRSEGVGGFLEQMAKQQGTTVAAVEKEFFQTARGSSLLKRFATSEEVAAMVVFV
HEECCCCCCCCCCHHHHHHHHHCCCEEHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHH
ASPLSSATNGAALRVEGGVVRSIL
HCCCCCCCCCCEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]