Click here to switch to the map view.

The map label for this gene is nirB [C]

Identifier: 116622980

GI number: 116622980

Start: 4915711

End: 4917012

Strand: Reverse

Name: nirB [C]

Synonym: Acid_3883

Alternate gene names: 116622980

Gene position: 4917012-4915711 (Counterclockwise)

Preceding gene: 116622981

Following gene: 116622979

Centisome position: 49.34

GC content: 66.74

Gene sequence:

>1302_bases
ATGGCACTGGTGGTGATCGGAGGCACGGCCGCGGGAATGAGCGCGGCGGCGCGGGCGCGGCGGATCGATCCACGTCTGGA
AATCATCGTTCTCGAAAAGGGTCCGGTCATCTCGCTGGGCGCTTGCGGGCTGCCCTATTTCGTGGAAGGCAAGATCCGCG
AGGCGAAAGACCTGACGGTATTCACGCCGGAGCGCTTCCGCAAGGAACGCAATGTGGACGTGCGTACCGGGGCGCGCGTG
ATCTCGATTGCCCATCCACGGCGCGAGGTGCTCCTGGAATCCGGCGCGCGCGTTGCCTACGAGAAGCTCGTGATCGCCAC
CGGCGCGCGCAGCGAGAGCCTGGGTATCGCCGGCGACACGCAGGAGCACGTGTTCCATATGCAAACCCTCGAAGATGCGC
AGCGCATGCGCGGATTCGTGCGGGAGCGCAAACCGAAGCGTGCGGTGGTGGTGGGCGCTGGCTACATAGGAGTCGAGGCC
GCCGACGCGCTGCGGCGCAATGGACTGCAGGTCACCATCCTCGAGTTCTCCCCATACGCACTCCTGCGTGCGGACGACGC
TTTCACCGCCGAGGTCCGCAAGCAACTGGAGCGTCACCGCGTGGAGTTGAAGACTGGCGTGCGGGTGACCGCGATCGAGC
CGGACAGCATCGCGGGCGTGCCCTGCGACATGGTGGTGATCGCCGCGGGCTTCAGGCCGGCCGCCACCCTGGCAGCCGAC
GCCGGGGTCGAACTCGGACGCAGCGGCGCGATCCGCACTGACGACCGCATGGAAACCAACGTCCGCGGCATCTTCGCGGC
GGGCGATTGCGCGGAAGTCCAGCACCTGGTGAGCGGACGCCCGACGTGGATTCCGCTCGGCACCACCGCCAACAAGTGCG
GGCGCGTGGCGGGCGCGAATGCCGCAGGTGCACGCGAGCGCTTCCCGGGCATCGTGGGGACCTCAATCGTCGGCATCTTT
GGCATGGGTTTCGCGACCACGGGATTTTCGGCCGCCGAGGCGCGGGCGGAAGGCTTCACGCCGGTGACCGCGCGGATTGA
GGCGCTCTCCCGGCCGCGCTATTACCAGGGCGTGAAGACCATCGTCGAACTGGTCGCCGATAAAGCGACGCGTCGTCTGC
TGGGCGGTAGCATAATCGGCGAAGACGGAACGGCCGGGAGGATCAACGTTCTCGCGACGGCGCTCACTGCTCGGCTGCGG
GTAGACGACTTCGAACAACTCGACCTGGCCTACTCGCCGCCATTCGCACCAGTGTGGGACCCGGTGCTAATCGCCGCACA
GCAGCTCATAAAGGAGTTGTAG

Upstream 100 bases:

>100_bases
CGAACAGGACGCCATCAACAGCTACTACCCTGACCGCAAGCTCAAGACCTTCGATATTCTGGATTTCGTGAACAAGATGA
AAACCGAGGAATAGCCGCCG

Downstream 100 bases:

>100_bases
GATCGCGCATCATCGTTGAATTTTAAGACATACAGATCTGATACTATCAGGGCATGTCGGAGAACGGAATGTTTTCATTG
ACCGGCCAGGTGGCGGTAGT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: NA

Alternate protein names: CoA-disulfide reductase; CoADR [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MALVVIGGTAAGMSAAARARRIDPRLEIIVLEKGPVISLGACGLPYFVEGKIREAKDLTVFTPERFRKERNVDVRTGARV
ISIAHPRREVLLESGARVAYEKLVIATGARSESLGIAGDTQEHVFHMQTLEDAQRMRGFVRERKPKRAVVVGAGYIGVEA
ADALRRNGLQVTILEFSPYALLRADDAFTAEVRKQLERHRVELKTGVRVTAIEPDSIAGVPCDMVVIAAGFRPAATLAAD
AGVELGRSGAIRTDDRMETNVRGIFAAGDCAEVQHLVSGRPTWIPLGTTANKCGRVAGANAAGARERFPGIVGTSIVGIF
GMGFATTGFSAAEARAEGFTPVTARIEALSRPRYYQGVKTIVELVADKATRRLLGGSIIGEDGTAGRINVLATALTARLR
VDDFEQLDLAYSPPFAPVWDPVLIAAQQLIKEL

Sequences:

>Translated_433_residues
MALVVIGGTAAGMSAAARARRIDPRLEIIVLEKGPVISLGACGLPYFVEGKIREAKDLTVFTPERFRKERNVDVRTGARV
ISIAHPRREVLLESGARVAYEKLVIATGARSESLGIAGDTQEHVFHMQTLEDAQRMRGFVRERKPKRAVVVGAGYIGVEA
ADALRRNGLQVTILEFSPYALLRADDAFTAEVRKQLERHRVELKTGVRVTAIEPDSIAGVPCDMVVIAAGFRPAATLAAD
AGVELGRSGAIRTDDRMETNVRGIFAAGDCAEVQHLVSGRPTWIPLGTTANKCGRVAGANAAGARERFPGIVGTSIVGIF
GMGFATTGFSAAEARAEGFTPVTARIEALSRPRYYQGVKTIVELVADKATRRLLGGSIIGEDGTAGRINVLATALTARLR
VDDFEQLDLAYSPPFAPVWDPVLIAAQQLIKEL
>Mature_432_residues
ALVVIGGTAAGMSAAARARRIDPRLEIIVLEKGPVISLGACGLPYFVEGKIREAKDLTVFTPERFRKERNVDVRTGARVI
SIAHPRREVLLESGARVAYEKLVIATGARSESLGIAGDTQEHVFHMQTLEDAQRMRGFVRERKPKRAVVVGAGYIGVEAA
DALRRNGLQVTILEFSPYALLRADDAFTAEVRKQLERHRVELKTGVRVTAIEPDSIAGVPCDMVVIAAGFRPAATLAADA
GVELGRSGAIRTDDRMETNVRGIFAAGDCAEVQHLVSGRPTWIPLGTTANKCGRVAGANAAGARERFPGIVGTSIVGIFG
MGFATTGFSAAEARAEGFTPVTARIEALSRPRYYQGVKTIVELVADKATRRLLGGSIIGEDGTAGRINVLATALTARLRV
DDFEQLDLAYSPPFAPVWDPVLIAAQQLIKEL

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=358, Percent_Identity=26.536312849162, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI226437568, Length=358, Percent_Identity=26.536312849162, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI65787454, Length=358, Percent_Identity=26.536312849162, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1789765, Length=346, Percent_Identity=25.4335260115607, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788892, Length=235, Percent_Identity=28.0851063829787, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081763, Length=218, Percent_Identity=28.4403669724771, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1786307, Length=435, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789065, Length=289, Percent_Identity=25.6055363321799, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=297, Percent_Identity=25.2525252525253, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639257, Length=310, Percent_Identity=26.1290322580645, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281359715, Length=310, Percent_Identity=26.1290322580645, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281359713, Length=310, Percent_Identity=26.1290322580645, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24639250, Length=310, Percent_Identity=26.1290322580645, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI18543267, Length=310, Percent_Identity=26.1290322580645, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24639252, Length=310, Percent_Identity=26.1290322580645, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585130, Length=350, Percent_Identity=22.2857142857143, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017758
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.14 [H]

Molecular weight: Translated: 46406; Mature: 46275

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVVIGGTAAGMSAAARARRIDPRLEIIVLEKGPVISLGACGLPYFVEGKIREAKDLTV
CEEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCEEEECCCCCCCCCEE
FTPERFRKERNVDVRTGARVISIAHPRREVLLESGARVAYEKLVIATGARSESLGIAGDT
ECHHHHHHHCCCCEECCCEEEEECCCHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCC
QEHVFHMQTLEDAQRMRGFVRERKPKRAVVVGAGYIGVEAADALRRNGLQVTILEFSPYA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEECHHHHHHHHHCCCEEEEEEECCEE
LLRADDAFTAEVRKQLERHRVELKTGVRVTAIEPDSIAGVPCDMVVIAAGFRPAATLAAD
EEECCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHH
AGVELGRSGAIRTDDRMETNVRGIFAAGDCAEVQHLVSGRPTWIPLGTTANKCGRVAGAN
CCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCEEECCCCHHHHCCCCCCC
AAGARERFPGIVGTSIVGIFGMGFATTGFSAAEARAEGFTPVTARIEALSRPRYYQGVKT
CCCCHHHCCCHHHHHHHHHHHCCHHHCCCCHHHHHCCCCCHHHHHHHHHCCCHHHHHHHH
IVELVADKATRRLLGGSIIGEDGTAGRINVLATALTARLRVDDFEQLDLAYSPPFAPVWD
HHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCH
PVLIAAQQLIKEL
HHHHHHHHHHHHC
>Mature Secondary Structure 
ALVVIGGTAAGMSAAARARRIDPRLEIIVLEKGPVISLGACGLPYFVEGKIREAKDLTV
EEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEECCCCCEEEECCCCCCCCCEE
FTPERFRKERNVDVRTGARVISIAHPRREVLLESGARVAYEKLVIATGARSESLGIAGDT
ECHHHHHHHCCCCEECCCEEEEECCCHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCC
QEHVFHMQTLEDAQRMRGFVRERKPKRAVVVGAGYIGVEAADALRRNGLQVTILEFSPYA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEECHHHHHHHHHCCCEEEEEEECCEE
LLRADDAFTAEVRKQLERHRVELKTGVRVTAIEPDSIAGVPCDMVVIAAGFRPAATLAAD
EEECCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHH
AGVELGRSGAIRTDDRMETNVRGIFAAGDCAEVQHLVSGRPTWIPLGTTANKCGRVAGAN
CCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCEEECCCCHHHHCCCCCCC
AAGARERFPGIVGTSIVGIFGMGFATTGFSAAEARAEGFTPVTARIEALSRPRYYQGVKT
CCCCHHHCCCHHHHHHHHHHHCCHHHCCCCHHHHHCCCCCHHHHHHHHHCCCHHHHHHHH
IVELVADKATRRLLGGSIIGEDGTAGRINVLATALTARLRVDDFEQLDLAYSPPFAPVWD
HHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCH
PVLIAAQQLIKEL
HHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA