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The map label for this gene is nth [H]

Identifier: 116622883

GI number: 116622883

Start: 4804766

End: 4805425

Strand: Direct

Name: nth [H]

Synonym: Acid_3784

Alternate gene names: 116622883

Gene position: 4804766-4805425 (Clockwise)

Preceding gene: 116622881

Following gene: 116622884

Centisome position: 48.21

GC content: 61.36

Gene sequence:

>660_bases
GTGAAACGTCCCAAAACCGCAGCCGAGCGCAAGGAGCGAATCGCCGAAATCCTGCTCACCTTGGACCGCATGTACCCCGA
AGCCACTTGCGCGCTCATCCACACCAACCCGTGGGAATTGCTCGTGGCGACGATTCTCTCGGCGCAGTGCACCGACAAAC
GCGTCAACATGGTGACGCCCGAGCTCTTCCGCAAGTACCCGACGCCCCAGGATTTCGCCGCCGTCGCACCGGAGGTGCTG
GCCAACGACATCCGCAGCACCGGCTTCTTCAACAACAAGGCGAAGTCTATCGTCGGCGCTGCCAGGCGAGTGACGCAGGA
ATTCGGCGGCAAGGTGCCGCGGACCATCCAAGAGTTGCTCACCATTCCGGGCGCGGCCCGCAAGACCGCTAACGTGGTGC
TTGGCACTGCCTACGGCATCGCTTCCGGAATCGTGGTGGACACGCACGTTTCACGCATTGCGCAGCGGCTTGATCTGACC
AAAGAAACCGACCCGGTGAAAATCGAGCAGGACCTGGTGAAGATCATCCCTCAGGATCGCTGGATCCGGTTTTCGCACCA
GATCATTCTGCACGGCCGGGCGCTGTGCATCGCGCGCAATCCGCTGTGCGACAAGTGCGATCTCAACCCCTTGTGCTACG
CGAAAGACAAGCGGGCATAG

Upstream 100 bases:

>100_bases
AATCGTCAAAGTGAAAGCCAGAACCGAGCGAAGCGCCAAAGGCGACCAACGCAGCGGCAAGCAGAACAAGGCGCGCGCGC
ATGACCAACTACGAGCTTAC

Downstream 100 bases:

>100_bases
TCTGCTGCCTACGTTAGCATTCACTAACTTTTACGAATCCGTTTCACCCCCGCGCGCGTTTATATAAGTAGCAGGAGGAA
GTACAAATGAAACGAGGGAT

Product: DNA-(apurinic or apyrimidinic site) lyase/endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKRPKTAAERKERIAEILLTLDRMYPEATCALIHTNPWELLVATILSAQCTDKRVNMVTPELFRKYPTPQDFAAVAPEVL
ANDIRSTGFFNNKAKSIVGAARRVTQEFGGKVPRTIQELLTIPGAARKTANVVLGTAYGIASGIVVDTHVSRIAQRLDLT
KETDPVKIEQDLVKIIPQDRWIRFSHQIILHGRALCIARNPLCDKCDLNPLCYAKDKRA

Sequences:

>Translated_219_residues
MKRPKTAAERKERIAEILLTLDRMYPEATCALIHTNPWELLVATILSAQCTDKRVNMVTPELFRKYPTPQDFAAVAPEVL
ANDIRSTGFFNNKAKSIVGAARRVTQEFGGKVPRTIQELLTIPGAARKTANVVLGTAYGIASGIVVDTHVSRIAQRLDLT
KETDPVKIEQDLVKIIPQDRWIRFSHQIILHGRALCIARNPLCDKCDLNPLCYAKDKRA
>Mature_219_residues
MKRPKTAAERKERIAEILLTLDRMYPEATCALIHTNPWELLVATILSAQCTDKRVNMVTPELFRKYPTPQDFAAVAPEVL
ANDIRSTGFFNNKAKSIVGAARRVTQEFGGKVPRTIQELLTIPGAARKTANVVLGTAYGIASGIVVDTHVSRIAQRLDLT
KETDPVKIEQDLVKIIPQDRWIRFSHQIILHGRALCIARNPLCDKCDLNPLCYAKDKRA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=29.608938547486, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI190358497, Length=161, Percent_Identity=29.1925465838509, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI6912520, Length=161, Percent_Identity=29.1925465838509, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI115298654, Length=162, Percent_Identity=30.2469135802469, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI115298652, Length=162, Percent_Identity=30.2469135802469, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI115298650, Length=162, Percent_Identity=30.2469135802469, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI115298648, Length=161, Percent_Identity=29.1925465838509, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787920, Length=202, Percent_Identity=38.1188118811881, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17554540, Length=119, Percent_Identity=33.6134453781513, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324530, Length=195, Percent_Identity=26.6666666666667, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45550361, Length=190, Percent_Identity=27.8947368421053, Blast_Score=85, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24471; Mature: 24471

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPKTAAERKERIAEILLTLDRMYPEATCALIHTNPWELLVATILSAQCTDKRVNMVTP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHCCCCH
ELFRKYPTPQDFAAVAPEVLANDIRSTGFFNNKAKSIVGAARRVTQEFGGKVPRTIQELL
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TIPGAARKTANVVLGTAYGIASGIVVDTHVSRIAQRLDLTKETDPVKIEQDLVKIIPQDR
CCCCCCHHHHHHEEECHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
WIRFSHQIILHGRALCIARNPLCDKCDLNPLCYAKDKRA
EEEHHHEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRPKTAAERKERIAEILLTLDRMYPEATCALIHTNPWELLVATILSAQCTDKRVNMVTP
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCHHCCCCH
ELFRKYPTPQDFAAVAPEVLANDIRSTGFFNNKAKSIVGAARRVTQEFGGKVPRTIQELL
HHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TIPGAARKTANVVLGTAYGIASGIVVDTHVSRIAQRLDLTKETDPVKIEQDLVKIIPQDR
CCCCCCHHHHHHEEECHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCC
WIRFSHQIILHGRALCIARNPLCDKCDLNPLCYAKDKRA
EEEHHHEEEEECCEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]