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The map label for this gene is lieA [H]

Identifier: 116622824

GI number: 116622824

Start: 4728830

End: 4730458

Strand: Direct

Name: lieA [H]

Synonym: Acid_3724

Alternate gene names: 116622824

Gene position: 4728830-4730458 (Clockwise)

Preceding gene: 116622823

Following gene: 116622825

Centisome position: 47.45

GC content: 65.07

Gene sequence:

>1629_bases
ATGAAGAAGTCTCTCGCCCTGCTTGCCGCCGTCTGCATCGTCTTCGCCCAGGAGTCCGTGGATTCCAGCGCGAACGATCG
CATTCGCAAAGAGGCCACGGAGCATTCCCAGGTGATGCACTCCCTGCACATGCTCACCGACCGCTACGGTCCCCGCCTCA
CCGGCTCGCCCAACCACGAAGCCGCCGCCAACTGGGCCGTCAAGCAGATGACCGAATGGGGCTTCAAGAACGCTCACCTC
GAGCCTTGGGACTTCGGCCACGCCGGCTGGATGAACGAGCATGCCGCCGGCTACATGACGTCGCCCGTCCGCGATAGCCT
CACGTTCGAGGTCCTCGCCTGGACGCCTTCCACCAAGGGTGTTGTCCATTCCGATGCCGTAGAAGTGGCGCCTCCGGCCA
CTCCAACCAAGGAGGAGTTGACCGCCTGGCTCCAGCAGAATGCCGCCAAGGTCCGGGGCAAGATCGTCCTCGTCGGCAAG
GCCCAGGTCGTCCCCGTCAATTTCACCCCTGCCGCCATGCGCCGCGACGACAAGACCATCAAGGAACAGTTCGATCCCAA
CAATCCCAACGCCGGGCGCGGCGGCTTCGGCGGACGCGGCGGCCGCGGCGCTCCCCCCGATCCTTCCAAAATGACCCCCG
CCCAGGTCAACGAACAGATCGATGCCTGGCTGGTCGCCAACAAGGCCCTCGTGCGTGTCAACGATGCGGCCCGCGAGCAC
GGCCAGATCCGCGCCTTCAACAACCGCACCTTTGACCTCGCCAAAGCCCCGCCCACCGTCGTCCTCCGCAATGAGGATTA
CGGCCGCATCGATCGTCTTCTCGCCGATGGTGAAACCGTCGGCCTCCAGTTCGACATCGCCAACCAGACCTTCCCTAAGG
GCAAGACCACGTACAACGTTGTTGCCGAGATTCCAGGCTCCGACAAGGCCGGCGAAATCGTCATGCTCGGCGGACATCTC
GATTCCTGGCACTCCGCCACCGGCGCCACCGACAACGCCATCGGCTCCGCCATGATGATGGAAGCCGCGCGTCTCATCCA
GGTTCTTGGACTGCGGCCCCGCCGCACCATCCGCGTGGCCCTTTGGTCCGGCGAAGAGGAAGGGCTCCTCGGTTCCCAGG
CTTACGTAAAACAGCACTTCGGCAGCTTCGAAAATCCCAAGCCCGAGTTCGCCACGCTGGACTGCTACTTCAATATCGAT
TCCGGCACCGGTCGCGTCCGCGGGGCCAGCGTCTTCGGTCCCCCCGAGGCCGCCGCGATTCTCCGTGCCGCGCTCGCGCC
CTTCGAAGATCTCGGTGTCGCCGGCGCCCTGCCCAACACCAGCCGCGCCACCGGCGGCACCGACAGCACTTCTTTCGCGA
ACGCCGGACTCGCCGGAATCGGCATGCAGCAGGATCCGATCGAATACCAGTCCGCCACCTGGCATACTAACCTGGATACC
TACGAGCGCATCGTCCCCGAGGACGCCATGAAGGCCGCTACTGTTATCGCCGCCGCCGTCTGGCACGTCGCCAACCGCGG
TGAGATGCTCCCCCGCTTCACGAAAGAAAAGATGCCGCCGCTTCCGCCGCCTGCTCCAGGTACGCGCCCGGCCACCACCC
CGGCCGCAAGCACCTCCGGCGCAAGATAG

Upstream 100 bases:

>100_bases
CGTGAGGGGAGCGCAACGCCAACCGCGTCCCTCCACATCATCCTCGTGGTAAACCGCGACCGTGAGGGAGCGATCTACAT
AAGCTATCCTAAGTCTCAAT

Downstream 100 bases:

>100_bases
GCTGCGTCGTCCTGTATTACACTGCTCTCGTTTGCTCGGCCGCAAACATCAGCACCGGAATATTCGCCGCCCCATCCGAG
CCGGGATCCCTCCGAGCCGC

Product: peptidase M28

Products: NA

Alternate protein names: LIE1 [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKKSLALLAAVCIVFAQESVDSSANDRIRKEATEHSQVMHSLHMLTDRYGPRLTGSPNHEAAANWAVKQMTEWGFKNAHL
EPWDFGHAGWMNEHAAGYMTSPVRDSLTFEVLAWTPSTKGVVHSDAVEVAPPATPTKEELTAWLQQNAAKVRGKIVLVGK
AQVVPVNFTPAAMRRDDKTIKEQFDPNNPNAGRGGFGGRGGRGAPPDPSKMTPAQVNEQIDAWLVANKALVRVNDAAREH
GQIRAFNNRTFDLAKAPPTVVLRNEDYGRIDRLLADGETVGLQFDIANQTFPKGKTTYNVVAEIPGSDKAGEIVMLGGHL
DSWHSATGATDNAIGSAMMMEAARLIQVLGLRPRRTIRVALWSGEEEGLLGSQAYVKQHFGSFENPKPEFATLDCYFNID
SGTGRVRGASVFGPPEAAAILRAALAPFEDLGVAGALPNTSRATGGTDSTSFANAGLAGIGMQQDPIEYQSATWHTNLDT
YERIVPEDAMKAATVIAAAVWHVANRGEMLPRFTKEKMPPLPPPAPGTRPATTPAASTSGAR

Sequences:

>Translated_542_residues
MKKSLALLAAVCIVFAQESVDSSANDRIRKEATEHSQVMHSLHMLTDRYGPRLTGSPNHEAAANWAVKQMTEWGFKNAHL
EPWDFGHAGWMNEHAAGYMTSPVRDSLTFEVLAWTPSTKGVVHSDAVEVAPPATPTKEELTAWLQQNAAKVRGKIVLVGK
AQVVPVNFTPAAMRRDDKTIKEQFDPNNPNAGRGGFGGRGGRGAPPDPSKMTPAQVNEQIDAWLVANKALVRVNDAAREH
GQIRAFNNRTFDLAKAPPTVVLRNEDYGRIDRLLADGETVGLQFDIANQTFPKGKTTYNVVAEIPGSDKAGEIVMLGGHL
DSWHSATGATDNAIGSAMMMEAARLIQVLGLRPRRTIRVALWSGEEEGLLGSQAYVKQHFGSFENPKPEFATLDCYFNID
SGTGRVRGASVFGPPEAAAILRAALAPFEDLGVAGALPNTSRATGGTDSTSFANAGLAGIGMQQDPIEYQSATWHTNLDT
YERIVPEDAMKAATVIAAAVWHVANRGEMLPRFTKEKMPPLPPPAPGTRPATTPAASTSGAR
>Mature_542_residues
MKKSLALLAAVCIVFAQESVDSSANDRIRKEATEHSQVMHSLHMLTDRYGPRLTGSPNHEAAANWAVKQMTEWGFKNAHL
EPWDFGHAGWMNEHAAGYMTSPVRDSLTFEVLAWTPSTKGVVHSDAVEVAPPATPTKEELTAWLQQNAAKVRGKIVLVGK
AQVVPVNFTPAAMRRDDKTIKEQFDPNNPNAGRGGFGGRGGRGAPPDPSKMTPAQVNEQIDAWLVANKALVRVNDAAREH
GQIRAFNNRTFDLAKAPPTVVLRNEDYGRIDRLLADGETVGLQFDIANQTFPKGKTTYNVVAEIPGSDKAGEIVMLGGHL
DSWHSATGATDNAIGSAMMMEAARLIQVLGLRPRRTIRVALWSGEEEGLLGSQAYVKQHFGSFENPKPEFATLDCYFNID
SGTGRVRGASVFGPPEAAAILRAALAPFEDLGVAGALPNTSRATGGTDSTSFANAGLAGIGMQQDPIEYQSATWHTNLDT
YERIVPEDAMKAATVIAAAVWHVANRGEMLPRFTKEKMPPLPPPAPGTRPATTPAASTSGAR

Specific function: A leucine-specific metalloprotease that plays a role in controlling the amount of leupeptin during colony development. Degrades leupeptin into three components, acetyl-leucine, leucine and argininal [H]

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28A subfamily [H]

Homologues:

Organism=Homo sapiens, GI7706387, Length=255, Percent_Identity=31.7647058823529, Blast_Score=111, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR007484
- InterPro:   IPR002884 [H]

Pfam domain/function: PF01483 P_proprotein; PF04389 Peptidase_M28 [H]

EC number: NA

Molecular weight: Translated: 58325; Mature: 58325

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLALLAAVCIVFAQESVDSSANDRIRKEATEHSQVMHSLHMLTDRYGPRLTGSPNHE
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
AAANWAVKQMTEWGFKNAHLEPWDFGHAGWMNEHAAGYMTSPVRDSLTFEVLAWTPSTKG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
VVHSDAVEVAPPATPTKEELTAWLQQNAAKVRGKIVLVGKAQVVPVNFTPAAMRRDDKTI
CEECCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHHCCHHHH
KEQFDPNNPNAGRGGFGGRGGRGAPPDPSKMTPAQVNEQIDAWLVANKALVRVNDAAREH
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEECCEEEEECHHHHHC
GQIRAFNNRTFDLAKAPPTVVLRNEDYGRIDRLLADGETVGLQFDIANQTFPKGKTTYNV
CCEEEECCCEEEECCCCCEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEE
VAEIPGSDKAGEIVMLGGHLDSWHSATGATDNAIGSAMMMEAARLIQVLGLRPRRTIRVA
EEECCCCCCCCCEEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LWSGEEEGLLGSQAYVKQHFGSFENPKPEFATLDCYFNIDSGTGRVRGASVFGPPEAAAI
EECCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCEECCCCCCCHHHHHH
LRAALAPFEDLGVAGALPNTSRATGGTDSTSFANAGLAGIGMQQDPIEYQSATWHTNLDT
HHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHH
YERIVPEDAMKAATVIAAAVWHVANRGEMLPRFTKEKMPPLPPPAPGTRPATTPAASTSG
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
AR
CC
>Mature Secondary Structure
MKKSLALLAAVCIVFAQESVDSSANDRIRKEATEHSQVMHSLHMLTDRYGPRLTGSPNHE
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
AAANWAVKQMTEWGFKNAHLEPWDFGHAGWMNEHAAGYMTSPVRDSLTFEVLAWTPSTKG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
VVHSDAVEVAPPATPTKEELTAWLQQNAAKVRGKIVLVGKAQVVPVNFTPAAMRRDDKTI
CEECCCEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCEEEEECCCHHHHHCCHHHH
KEQFDPNNPNAGRGGFGGRGGRGAPPDPSKMTPAQVNEQIDAWLVANKALVRVNDAAREH
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEEECCEEEEECHHHHHC
GQIRAFNNRTFDLAKAPPTVVLRNEDYGRIDRLLADGETVGLQFDIANQTFPKGKTTYNV
CCEEEECCCEEEECCCCCEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEE
VAEIPGSDKAGEIVMLGGHLDSWHSATGATDNAIGSAMMMEAARLIQVLGLRPRRTIRVA
EEECCCCCCCCCEEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LWSGEEEGLLGSQAYVKQHFGSFENPKPEFATLDCYFNIDSGTGRVRGASVFGPPEAAAI
EECCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCEECCCCCCCHHHHHH
LRAALAPFEDLGVAGALPNTSRATGGTDSTSFANAGLAGIGMQQDPIEYQSATWHTNLDT
HHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHH
YERIVPEDAMKAATVIAAAVWHVANRGEMLPRFTKEKMPPLPPPAPGTRPATTPAASTSG
HHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA