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The map label for this gene is lieA [H]

Identifier: 116622653

GI number: 116622653

Start: 4498487

End: 4500061

Strand: Direct

Name: lieA [H]

Synonym: Acid_3552

Alternate gene names: 116622653

Gene position: 4498487-4500061 (Clockwise)

Preceding gene: 116622650

Following gene: 116622654

Centisome position: 45.14

GC content: 64.57

Gene sequence:

>1575_bases
GTGCGAGCCGCCGATACCGAGAAGGTCGATCTGGGAGTGCTGCACCGCATCAAGAGCGAGGCCTTCGCCCGGAATTCGCA
GGTTATGGACACGCTGTTCTACCTGACCGATGTCTATGGACCGCGATTGACCGGTTCGCCCAACATCAACCATGCCGCGG
AGTGGTCGGTGAAAAAGATGCAGGAGTGGGGCCTGGCCAATGTGAAGACGGAGAAGTGGGGACCGTTCGGCCGCGGTTGG
GCCGCGACGCGCTTCTCGGCGATGATGAAGGAGCCGGAATTCCAGCCCCTCATCGGCTTCCAACAACCCTGGTCGCCAGG
GACCAACGGCGCGGTCTCCGGACAGGCCGTGATGGCCGTCATCACCGGCCCCGATGATCTCGAAAAGTTCAAGGGCAAAT
TGAAGGGTAAGATCGTGCTGAGCGCGGCGATGCACGCGTCGCAGCTGAATGAAGATGCACTCTCGCACCGCCTGACCGAT
GCCGAGCTCGACGCCGCGGCCACGGCGCCCGATCCCTCGATGGGCAATCCGGCGATGTTGCCGCTCGGCTTCTCGCGTCC
TGGCGCGGGACGCGGACCCGCAGCAGCGCCCGGGCGTGGTGCGGCGGGCGGCCGCGGCGCCAATCCCCGCGCGTTCCGCG
CGCAGTTGAATAAGTTCCTGACCGATGAAGGCGTCCTGGTGGTGGTCTCGCCGGGCAACGGCCCCGATGGCGGCACCATC
ATGGGTTCGGCCGCCGGCTCGCAGAATCCGGACGAAGCGCTGCCGCCGCCTTCAGTCCTGGTGACCAACGAACACTACAA
CCGGATCGCGCGCCTGATCGAAAAGAATATCCCTGTGACGCTGGAATTCGACCTCGGCGCCAAGTTTACCGAGCCCGGCG
ATTCCTTCAACCTGGTCGCGGAGATTCCGGGCACCGACCCCAACGCCGGGTTCGTCATGCTGGGCGGCCATTTCGATTCC
TGGACCGGCGGCACCGGCGCTACCGACAATGCCGCGGGCGCGGCCGTCGCGATGGAAGCCATGCGCATTCTCAAAACATT
GGACCTGAAGATGCCGCGCACCATCCGGCTGGGCCTGTGGACCGCCGAAGAGCAGGGCCTGCTCGGCTCGCGCGCGTACG
TGAAGGAGCACTTCGCCGACCCCGCGGACATGAAGCCGAAGCCCGAGCATTCCAAACTCGCGGGCTACTTCAACCTGGAC
AACGGCAGCGGAAAGGTTCGCGGGATCTATATTCAGGATAACGACCAGGTGCGACCGGTCTTCGAAAGCTGGGTAGCGCC
GTTGAAGGACCTGGGCGTGACCACTCTGACCATCCGTAACACCGGCAGCACCGATCACATGTCGTTCGACGGCGTCGGCC
TGCCCGCCTTCCAGTTCATTCAGGACCCACTGGAATACGGCACGCGCACCCACCACTCCAATATGGACGTGTATGACCAT
CTGCAATCCGGCGACCTCGAGCAGGCATCCGCCGTGATGGCCTGGTTCGTCTACAACACGGCGACCCGGCCCGAGATGCT
GCCGCGCAAACCAATGCCGAAGCCGGGCGCTGGGCGCGGAGGCCGCGGTGAGTAA

Upstream 100 bases:

>100_bases
GGCAAGCGCGATTTGATCGGTTCCCATTCGCCGTGTTACAGTCCCCCTCATGCGCAAATGGTTGGTTGGAAGTGCCGGGA
TACTCGGGGTTTGTCTGTTG

Downstream 100 bases:

>100_bases
CTTATCTTAAGAATTGTTAAACCGCTTGCGGTAGCCCGCAAGTGCCCCCGCTTGGAGTATCCTGGATCATGCGTTTTTTA
GCCACTGTACTGTTGCTCGC

Product: peptidase M28

Products: NA

Alternate protein names: LIE1 [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MRAADTEKVDLGVLHRIKSEAFARNSQVMDTLFYLTDVYGPRLTGSPNINHAAEWSVKKMQEWGLANVKTEKWGPFGRGW
AATRFSAMMKEPEFQPLIGFQQPWSPGTNGAVSGQAVMAVITGPDDLEKFKGKLKGKIVLSAAMHASQLNEDALSHRLTD
AELDAAATAPDPSMGNPAMLPLGFSRPGAGRGPAAAPGRGAAGGRGANPRAFRAQLNKFLTDEGVLVVVSPGNGPDGGTI
MGSAAGSQNPDEALPPPSVLVTNEHYNRIARLIEKNIPVTLEFDLGAKFTEPGDSFNLVAEIPGTDPNAGFVMLGGHFDS
WTGGTGATDNAAGAAVAMEAMRILKTLDLKMPRTIRLGLWTAEEQGLLGSRAYVKEHFADPADMKPKPEHSKLAGYFNLD
NGSGKVRGIYIQDNDQVRPVFESWVAPLKDLGVTTLTIRNTGSTDHMSFDGVGLPAFQFIQDPLEYGTRTHHSNMDVYDH
LQSGDLEQASAVMAWFVYNTATRPEMLPRKPMPKPGAGRGGRGE

Sequences:

>Translated_524_residues
MRAADTEKVDLGVLHRIKSEAFARNSQVMDTLFYLTDVYGPRLTGSPNINHAAEWSVKKMQEWGLANVKTEKWGPFGRGW
AATRFSAMMKEPEFQPLIGFQQPWSPGTNGAVSGQAVMAVITGPDDLEKFKGKLKGKIVLSAAMHASQLNEDALSHRLTD
AELDAAATAPDPSMGNPAMLPLGFSRPGAGRGPAAAPGRGAAGGRGANPRAFRAQLNKFLTDEGVLVVVSPGNGPDGGTI
MGSAAGSQNPDEALPPPSVLVTNEHYNRIARLIEKNIPVTLEFDLGAKFTEPGDSFNLVAEIPGTDPNAGFVMLGGHFDS
WTGGTGATDNAAGAAVAMEAMRILKTLDLKMPRTIRLGLWTAEEQGLLGSRAYVKEHFADPADMKPKPEHSKLAGYFNLD
NGSGKVRGIYIQDNDQVRPVFESWVAPLKDLGVTTLTIRNTGSTDHMSFDGVGLPAFQFIQDPLEYGTRTHHSNMDVYDH
LQSGDLEQASAVMAWFVYNTATRPEMLPRKPMPKPGAGRGGRGE
>Mature_524_residues
MRAADTEKVDLGVLHRIKSEAFARNSQVMDTLFYLTDVYGPRLTGSPNINHAAEWSVKKMQEWGLANVKTEKWGPFGRGW
AATRFSAMMKEPEFQPLIGFQQPWSPGTNGAVSGQAVMAVITGPDDLEKFKGKLKGKIVLSAAMHASQLNEDALSHRLTD
AELDAAATAPDPSMGNPAMLPLGFSRPGAGRGPAAAPGRGAAGGRGANPRAFRAQLNKFLTDEGVLVVVSPGNGPDGGTI
MGSAAGSQNPDEALPPPSVLVTNEHYNRIARLIEKNIPVTLEFDLGAKFTEPGDSFNLVAEIPGTDPNAGFVMLGGHFDS
WTGGTGATDNAAGAAVAMEAMRILKTLDLKMPRTIRLGLWTAEEQGLLGSRAYVKEHFADPADMKPKPEHSKLAGYFNLD
NGSGKVRGIYIQDNDQVRPVFESWVAPLKDLGVTTLTIRNTGSTDHMSFDGVGLPAFQFIQDPLEYGTRTHHSNMDVYDH
LQSGDLEQASAVMAWFVYNTATRPEMLPRKPMPKPGAGRGGRGE

Specific function: A leucine-specific metalloprotease that plays a role in controlling the amount of leupeptin during colony development. Degrades leupeptin into three components, acetyl-leucine, leucine and argininal [H]

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28A subfamily [H]

Homologues:

Organism=Homo sapiens, GI7706387, Length=272, Percent_Identity=31.25, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR007484
- InterPro:   IPR002884 [H]

Pfam domain/function: PF01483 P_proprotein; PF04389 Peptidase_M28 [H]

EC number: NA

Molecular weight: Translated: 56232; Mature: 56232

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAADTEKVDLGVLHRIKSEAFARNSQVMDTLFYLTDVYGPRLTGSPNINHAAEWSVKKM
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
QEWGLANVKTEKWGPFGRGWAATRFSAMMKEPEFQPLIGFQQPWSPGTNGAVSGQAVMAV
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ITGPDDLEKFKGKLKGKIVLSAAMHASQLNEDALSHRLTDAELDAAATAPDPSMGNPAML
EECCHHHHHHHHHHCCEEEEEHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
PLGFSRPGAGRGPAAAPGRGAAGGRGANPRAFRAQLNKFLTDEGVLVVVSPGNGPDGGTI
EECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEE
MGSAAGSQNPDEALPPPSVLVTNEHYNRIARLIEKNIPVTLEFDLGAKFTEPGDSFNLVA
EECCCCCCCCHHCCCCCCEEEECHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEE
EIPGTDPNAGFVMLGGHFDSWTGGTGATDNAAGAAVAMEAMRILKTLDLKMPRTIRLGLW
ECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
TAEEQGLLGSRAYVKEHFADPADMKPKPEHSKLAGYFNLDNGSGKVRGIYIQDNDQVRPV
ECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHH
FESWVAPLKDLGVTTLTIRNTGSTDHMSFDGVGLPAFQFIQDPLEYGTRTHHSNMDVYDH
HHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
LQSGDLEQASAVMAWFVYNTATRPEMLPRKPMPKPGAGRGGRGE
HCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRAADTEKVDLGVLHRIKSEAFARNSQVMDTLFYLTDVYGPRLTGSPNINHAAEWSVKKM
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
QEWGLANVKTEKWGPFGRGWAATRFSAMMKEPEFQPLIGFQQPWSPGTNGAVSGQAVMAV
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ITGPDDLEKFKGKLKGKIVLSAAMHASQLNEDALSHRLTDAELDAAATAPDPSMGNPAML
EECCHHHHHHHHHHCCEEEEEHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
PLGFSRPGAGRGPAAAPGRGAAGGRGANPRAFRAQLNKFLTDEGVLVVVSPGNGPDGGTI
EECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEE
MGSAAGSQNPDEALPPPSVLVTNEHYNRIARLIEKNIPVTLEFDLGAKFTEPGDSFNLVA
EECCCCCCCCHHCCCCCCEEEECHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEE
EIPGTDPNAGFVMLGGHFDSWTGGTGATDNAAGAAVAMEAMRILKTLDLKMPRTIRLGLW
ECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEE
TAEEQGLLGSRAYVKEHFADPADMKPKPEHSKLAGYFNLDNGSGKVRGIYIQDNDQVRPV
ECCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHH
FESWVAPLKDLGVTTLTIRNTGSTDHMSFDGVGLPAFQFIQDPLEYGTRTHHSNMDVYDH
HHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHH
LQSGDLEQASAVMAWFVYNTATRPEMLPRKPMPKPGAGRGGRGE
HCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA