Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 116622643

GI number: 116622643

Start: 4488100

End: 4488453

Strand: Reverse

Name: metG [H]

Synonym: Acid_3542

Alternate gene names: 116622643

Gene position: 4488453-4488100 (Counterclockwise)

Preceding gene: 116622644

Following gene: 116622642

Centisome position: 45.04

GC content: 63.28

Gene sequence:

>354_bases
ATGAATCCCGCGCCCATTAAACCGACCATCACCGCCGACCTGCTGGAGAAGCTCGATATCCGCGCCGGCACCATTCTCGC
GGTGGAAGATGTGCCGAACTCCGACAAGCTGGTGCAGCTCCGCGTCAGCTTTGGCGATCACGAGCGCATCATCCTCTCCG
GCATGAAGCAGGAGCGCGCCAACCCGCGCGAGATTGCAGGCCGGCAGGCGCTCTTCGTAGTCAACCTGGAACCGCGGAAG
ATGCGCGGCATTGTATCGCAGGGAATGCTCTTCGACCTCGGCTTCGCCGACGGCATCACGCCCGTGCTCGCGACCCCCGA
GTCGCCCGTACCGGATGGAACACGTGCAGGATAA

Upstream 100 bases:

>100_bases
ATCGACCATGACCCTGCGAAGCCCGCTTGCGCCAAGGGAATCGCCGACCTTTATACCCTGATCGGGCAGACCGCCCGCGC
CAATCAATGGATGTCCTTCT

Downstream 100 bases:

>100_bases
GATGCCATCCAATTCCCCGGCATGGCTGCGGCCGGCGATCGTCGCGCTCGCGGCGTTGCTATTGTTGGCCTGCTTCTCCA
CCGAAGTCGCCGACGCGGAT

Product: tRNA-binding domain-containing protein

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MNPAPIKPTITADLLEKLDIRAGTILAVEDVPNSDKLVQLRVSFGDHERIILSGMKQERANPREIAGRQALFVVNLEPRK
MRGIVSQGMLFDLGFADGITPVLATPESPVPDGTRAG

Sequences:

>Translated_117_residues
MNPAPIKPTITADLLEKLDIRAGTILAVEDVPNSDKLVQLRVSFGDHERIILSGMKQERANPREIAGRQALFVVNLEPRK
MRGIVSQGMLFDLGFADGITPVLATPESPVPDGTRAG
>Mature_117_residues
MNPAPIKPTITADLLEKLDIRAGTILAVEDVPNSDKLVQLRVSFGDHERIILSGMKQERANPREIAGRQALFVVNLEPRK
MRGIVSQGMLFDLGFADGITPVLATPESPVPDGTRAG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788432, Length=90, Percent_Identity=40, Blast_Score=72, Evalue=6e-15,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 12672; Mature: 12672

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPAPIKPTITADLLEKLDIRAGTILAVEDVPNSDKLVQLRVSFGDHERIILSGMKQERA
CCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCEEEEECCHHHCC
NPREIAGRQALFVVNLEPRKMRGIVSQGMLFDLGFADGITPVLATPESPVPDGTRAG
CHHHCCCCEEEEEEECCCHHHHHHHHCCCEEEECCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNPAPIKPTITADLLEKLDIRAGTILAVEDVPNSDKLVQLRVSFGDHERIILSGMKQERA
CCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCCEEEEECCHHHCC
NPREIAGRQALFVVNLEPRKMRGIVSQGMLFDLGFADGITPVLATPESPVPDGTRAG
CHHHCCCCEEEEEEECCCHHHHHHHHCCCEEEECCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA