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The map label for this gene is rspA [H]

Identifier: 116622625

GI number: 116622625

Start: 4462819

End: 4464159

Strand: Direct

Name: rspA [H]

Synonym: Acid_3524

Alternate gene names: 116622625

Gene position: 4462819-4464159 (Clockwise)

Preceding gene: 116622624

Following gene: 116622626

Centisome position: 44.78

GC content: 62.64

Gene sequence:

>1341_bases
ATGTCGATACAACGCCGCGACTTCTTGCGGGCATTCGGGACGGCCGGGCTGGCAGGCCTCGCCGTCCCGCGTGTGCAGCA
GGCTCAAGAGCAGGCTGCGCGCGCCACTCGTGGATTGCCGATTCCGAAAATCAAAGACATCAGCGTGATTGAATGCCAGC
CGGCGGGTGTGCGGCTGACGGTGGTCAAGATCACGACCGACCAGGACGGGTTATACGGCTACGGCTGTGCCACGTTCACG
CAGCGCGCGGACCTGGTGAAGCCGGCCGTGGAGCGTTATCTCAAACCGTTTCTGATGGGGAAGACGACGGACCGGATCGA
AGACATCTGGCAATCCTGCTACGACAGTTCGTACTGGAAGAACGGCCCGGTGCTCAACAACGCGATCAGCGGCATCGATC
AGGCGTTGTGGGACATCAAGGGCAGACAGGCGGGGCTGCCGGTATACCAGCTGGCCGGCGGCAAGTGCCGGGAAGCGGCC
GATTGCTACGCGCACGCCGACGGCGCCGATTTTCCGAATGTGGTGGAAGCCGCGAAGCGGTACATGGCGCAGGGATTCCG
GCATGTGCGCGTGCAGATCGGCGTGCCGGGCATGGCCGGGTACGGCAGTTCGCGCGGCGACACCAAGGTTCAGGCGCTGC
ACAGCAAGCCGGTTTTCGAGCCGGCCTATTACGTGCGGCGCGCGTTGAAGCTGTTCGAAGTCTGCCGCACGGAACTGGGC
GAAGAGATCGAACTGCTGCACGATATGCACGAACGCGTATCGCCCAACCAGGCGGTTCAGTTTGTGAAAGACGCCGAGAA
GTTCAAGATGTTCTTCATGGAAGATCCGCTGTCGCCGGAGGACATCGCGTATTTCCGGCAGATCCGGCAGCAGTGCGCGA
CACCGATCGCGATGGGCGAACTGTTCAATAGCCCGCACGAGTGGACCCCATTGATCAGCGAGCGGCTGATCGACTATATC
CGCGTTCACGTATCGCAGGCGGGCGGATTCACGCCGGCGCGCAAGATCGCGATCCTCGGCGAAACCTTCGGCGTGAAGAC
AGCGTGGCACGGGCCCGGCGACGTTTCGCCAATCGGGCACATGGCGAACGTCACGCTGGACCTGGTGAGCTACAACTTCG
GTATTCAAGAGTACTCGCCCTTCAACGGGCGGACGCAGGAGATCTTCTCGGGCTGCCCGGTGATGAAGGACGGCTATCTG
TATGCCAGCGAGAAACCCGGTTGGGGCATCGAAGTGGACGAGAAGCTGGCGGCGAAGGCGCCGTTCACGGATCGTCCGGA
TGGCCTGAACGGCGGCTGGGGTGAGATCCGCCGCCGCGACGGCACGGTCATCAAACAGTAG

Upstream 100 bases:

>100_bases
CGCAATTGCAATCGCCGTTGTTCGGCAAGCTCACGAGCAATGGCGATCTGTTGAACACGCCGCGTACCATGCAGCTCGCA
GCTAAACTATACTTCTAACC

Downstream 100 bases:

>100_bases
GCAGGCATGACGGGCACCGACTACCAGCGGTTGGCGCTCAGTCTTCCGGGAGCCACGGTAGGCTCGCATATGGGGAACGC
GGACTTCCGCGTGGGTGGGC

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSIQRRDFLRAFGTAGLAGLAVPRVQQAQEQAARATRGLPIPKIKDISVIECQPAGVRLTVVKITTDQDGLYGYGCATFT
QRADLVKPAVERYLKPFLMGKTTDRIEDIWQSCYDSSYWKNGPVLNNAISGIDQALWDIKGRQAGLPVYQLAGGKCREAA
DCYAHADGADFPNVVEAAKRYMAQGFRHVRVQIGVPGMAGYGSSRGDTKVQALHSKPVFEPAYYVRRALKLFEVCRTELG
EEIELLHDMHERVSPNQAVQFVKDAEKFKMFFMEDPLSPEDIAYFRQIRQQCATPIAMGELFNSPHEWTPLISERLIDYI
RVHVSQAGGFTPARKIAILGETFGVKTAWHGPGDVSPIGHMANVTLDLVSYNFGIQEYSPFNGRTQEIFSGCPVMKDGYL
YASEKPGWGIEVDEKLAAKAPFTDRPDGLNGGWGEIRRRDGTVIKQ

Sequences:

>Translated_446_residues
MSIQRRDFLRAFGTAGLAGLAVPRVQQAQEQAARATRGLPIPKIKDISVIECQPAGVRLTVVKITTDQDGLYGYGCATFT
QRADLVKPAVERYLKPFLMGKTTDRIEDIWQSCYDSSYWKNGPVLNNAISGIDQALWDIKGRQAGLPVYQLAGGKCREAA
DCYAHADGADFPNVVEAAKRYMAQGFRHVRVQIGVPGMAGYGSSRGDTKVQALHSKPVFEPAYYVRRALKLFEVCRTELG
EEIELLHDMHERVSPNQAVQFVKDAEKFKMFFMEDPLSPEDIAYFRQIRQQCATPIAMGELFNSPHEWTPLISERLIDYI
RVHVSQAGGFTPARKIAILGETFGVKTAWHGPGDVSPIGHMANVTLDLVSYNFGIQEYSPFNGRTQEIFSGCPVMKDGYL
YASEKPGWGIEVDEKLAAKAPFTDRPDGLNGGWGEIRRRDGTVIKQ
>Mature_445_residues
SIQRRDFLRAFGTAGLAGLAVPRVQQAQEQAARATRGLPIPKIKDISVIECQPAGVRLTVVKITTDQDGLYGYGCATFTQ
RADLVKPAVERYLKPFLMGKTTDRIEDIWQSCYDSSYWKNGPVLNNAISGIDQALWDIKGRQAGLPVYQLAGGKCREAAD
CYAHADGADFPNVVEAAKRYMAQGFRHVRVQIGVPGMAGYGSSRGDTKVQALHSKPVFEPAYYVRRALKLFEVCRTELGE
EIELLHDMHERVSPNQAVQFVKDAEKFKMFFMEDPLSPEDIAYFRQIRQQCATPIAMGELFNSPHEWTPLISERLIDYIR
VHVSQAGGFTPARKIAILGETFGVKTAWHGPGDVSPIGHMANVTLDLVSYNFGIQEYSPFNGRTQEIFSGCPVMKDGYLY
ASEKPGWGIEVDEKLAAKAPFTDRPDGLNGGWGEIRRRDGTVIKQ

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=375, Percent_Identity=42.4, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI48994953, Length=413, Percent_Identity=31.4769975786925, Blast_Score=166, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 49603; Mature: 49472

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQRRDFLRAFGTAGLAGLAVPRVQQAQEQAARATRGLPIPKIKDISVIECQPAGVRLT
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEE
VVKITTDQDGLYGYGCATFTQRADLVKPAVERYLKPFLMGKTTDRIEDIWQSCYDSSYWK
EEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
NGPVLNNAISGIDQALWDIKGRQAGLPVYQLAGGKCREAADCYAHADGADFPNVVEAAKR
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHECCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
YMAQGFRHVRVQIGVPGMAGYGSSRGDTKVQALHSKPVFEPAYYVRRALKLFEVCRTELG
HHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EEIELLHDMHERVSPNQAVQFVKDAEKFKMFFMEDPLSPEDIAYFRQIRQQCATPIAMGE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
LFNSPHEWTPLISERLIDYIRVHVSQAGGFTPARKIAILGETFGVKTAWHGPGDVSPIGH
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCEEECCCCCCCCCCH
MANVTLDLVSYNFGIQEYSPFNGRTQEIFSGCPVMKDGYLYASEKPGWGIEVDEKLAAKA
HHHHEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHCCC
PFTDRPDGLNGGWGEIRRRDGTVIKQ
CCCCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
SIQRRDFLRAFGTAGLAGLAVPRVQQAQEQAARATRGLPIPKIKDISVIECQPAGVRLT
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEE
VVKITTDQDGLYGYGCATFTQRADLVKPAVERYLKPFLMGKTTDRIEDIWQSCYDSSYWK
EEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
NGPVLNNAISGIDQALWDIKGRQAGLPVYQLAGGKCREAADCYAHADGADFPNVVEAAKR
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHECCCCCHHHHHHHHHCCCCCCCCHHHHHHHH
YMAQGFRHVRVQIGVPGMAGYGSSRGDTKVQALHSKPVFEPAYYVRRALKLFEVCRTELG
HHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
EEIELLHDMHERVSPNQAVQFVKDAEKFKMFFMEDPLSPEDIAYFRQIRQQCATPIAMGE
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHH
LFNSPHEWTPLISERLIDYIRVHVSQAGGFTPARKIAILGETFGVKTAWHGPGDVSPIGH
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEECCCCCCEEECCCCCCCCCCH
MANVTLDLVSYNFGIQEYSPFNGRTQEIFSGCPVMKDGYLYASEKPGWGIEVDEKLAAKA
HHHHEEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCCHHHHCCC
PFTDRPDGLNGGWGEIRRRDGTVIKQ
CCCCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]