Click here to switch to the map view.

The map label for this gene is hmp [H]

Identifier: 116622578

GI number: 116622578

Start: 4394667

End: 4396421

Strand: Reverse

Name: hmp [H]

Synonym: Acid_3476

Alternate gene names: 116622578

Gene position: 4396421-4394667 (Counterclockwise)

Preceding gene: 116622582

Following gene: 116622577

Centisome position: 44.12

GC content: 63.02

Gene sequence:

>1755_bases
ATGGGACGGCTTCTTTCGGTTAACGTAGGGCTTCCCCGCGACATAACCTGGCGGGGCGAAACCGTGCACACAGGCATCTG
GAAGCACCCGGTGAGCGGCAGGCAGATGGTCCGTCGCCTGAATATTGCCGGCGATGGTCAAGGTGATCTTGCCGGTCACG
GCGGCGAACACCGCGCGGTACTTGTCTACCAATCCGCGTCCTATCGGTACTGGGAGTCTCAACTCAACCGCAAGAATCTC
ACATACGGACAATTCGGGGAAAACTTCACCGTCGATGGCTTACCGGACGACGAAGTCTTCATCGGCGATCGCTATCGCAT
AGGCGGCGCTCTCTTCGAAGTTACGCAACCTCGCGTAACCTGCTACCGCGTCGGCATACGAATGGAAGAACCGCAAATGG
CCGCGCTGCTGGTCTCGCATCACCGGCCTGGCTTCTATCTCCGCGTACTCGAGGAAGGAGACGTCGGCGGCGGCGACGAA
ATCACCAAGGTCGCCGAGGGACCCGAACGTATCACGGTAGCTGCCATCGACGCCCTGCTCTATTTGCCGGGCCACCCGCG
CGAGCAATTGGAGAGCGCGTTGCGTATCCCCGCCCTCCCCCCGGGGTGGAAAGGCTCACTGCAGGCGCTTCTCAATCGCG
GAAACGCCGAGTCGGGAAACCCGGGCCTCACAGGTTTGGCCGGGGCACCGCCGGCCTGGTCAGGCTTTCGTTCCTTGCGC
GTCTCCAAGGTGCAACAGGAATGTGCCTCCGTGTTTTCCTTGTGCTTCGAACCGGTGGACGGCCCGGCTTTGCCCGCCGC
GCTGCCCGGTCAGTTTCTGGTCTTCAGGCTGCACACCAAACCCGGCCTTCCGCCAATCCTTCGCAACTACTCCCTCTCCA
GCGCTCCAGGCTCAAGTACGTATCGCGTGAGCGTCAAACAGGAAGTCAATGGCGCCGGCAGTTCGTTCCTGCTCAATCAC
GTGAATGCGGGCGACGTCCTCGAAGTGAGCGCGCCAAGGGGCGATTTCACGCTACTTGGCGGCGATGGACCCGTCGTTCT
GGTGAGTGCCGGCATCGGCGCTACTCCCGTGCTCGCGATGCTTCACGCGCTCGCTTCCACCAGGTCCCCTCGCGAAGTCT
GGTGGCTGTATGGCGCGCGAGACCGCAAAGAACATCCCTTCGCGAAGGAGTCGCGCGAACTCCTGCAATCGCTCGCGCGG
GCTCAAAGCCACATCGTCTACAGCAGGCCCCAGCCTGACGATCGGCTGGGCCTCGATTACGATTCGCCCGGCCACCTCGA
CATTCCCCTACTCGACCGCCTCGGCGTGCCTCAAAACGCCGATTTCTATCTGTGTGGACCGCCATCTTTCCTGGGCAGTT
TCACGGCGGACCTCGCTAAACGAGGCGTCGATTCCGCCCGTATCCACACGGAGATCTTCGGGCCGCGGGAGTCGATCACC
CCTGGCATCGCACCCGCACCGCGACCTCCTGCGCACCTCCCTGCGGGAGCGCCGGGCACCGGTCCGCAGGTTGCCTTCGC
CCGAAGCGGCATCACTGTCCCATGGAACCCAACCTTCCGCAGTCTGCTCGAATTGGCCGAGGCCTGCGACGTTCCCGCAA
GATGGTCATGTCGCACCGGCGTCTGCCACACCTGCGAATGCGCTCTGATCGGAGGCACCGTCGAGTATCAAACCCTCCCC
CTGGAACCGCCGGCCGAGGGCAACCTCCTGGTCTGTTGCTCGCAGCCGCAGGGTGACGTAGAGATCGACCTCTAG

Upstream 100 bases:

>100_bases
CGCCCCACCGGTACAAGTCACTAACAATACATTCGCGCCTCGCATCGGTGAATCATGCGGAGTCACCTGACCATCGCTTA
CCCTTGGGCAGGAGCACTCA

Downstream 100 bases:

>100_bases
GTGGTGCGGCGAGGCCGATCAGAGCGATCGCTTGATCCTTGTCTGCGGCCTGCCGCGATGTACTACTGTGTATTTGAGTG
TCCGCACGCCGCGAAGATTC

Product: MOSC domain-containing protein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MGRLLSVNVGLPRDITWRGETVHTGIWKHPVSGRQMVRRLNIAGDGQGDLAGHGGEHRAVLVYQSASYRYWESQLNRKNL
TYGQFGENFTVDGLPDDEVFIGDRYRIGGALFEVTQPRVTCYRVGIRMEEPQMAALLVSHHRPGFYLRVLEEGDVGGGDE
ITKVAEGPERITVAAIDALLYLPGHPREQLESALRIPALPPGWKGSLQALLNRGNAESGNPGLTGLAGAPPAWSGFRSLR
VSKVQQECASVFSLCFEPVDGPALPAALPGQFLVFRLHTKPGLPPILRNYSLSSAPGSSTYRVSVKQEVNGAGSSFLLNH
VNAGDVLEVSAPRGDFTLLGGDGPVVLVSAGIGATPVLAMLHALASTRSPREVWWLYGARDRKEHPFAKESRELLQSLAR
AQSHIVYSRPQPDDRLGLDYDSPGHLDIPLLDRLGVPQNADFYLCGPPSFLGSFTADLAKRGVDSARIHTEIFGPRESIT
PGIAPAPRPPAHLPAGAPGTGPQVAFARSGITVPWNPTFRSLLELAEACDVPARWSCRTGVCHTCECALIGGTVEYQTLP
LEPPAEGNLLVCCSQPQGDVEIDL

Sequences:

>Translated_584_residues
MGRLLSVNVGLPRDITWRGETVHTGIWKHPVSGRQMVRRLNIAGDGQGDLAGHGGEHRAVLVYQSASYRYWESQLNRKNL
TYGQFGENFTVDGLPDDEVFIGDRYRIGGALFEVTQPRVTCYRVGIRMEEPQMAALLVSHHRPGFYLRVLEEGDVGGGDE
ITKVAEGPERITVAAIDALLYLPGHPREQLESALRIPALPPGWKGSLQALLNRGNAESGNPGLTGLAGAPPAWSGFRSLR
VSKVQQECASVFSLCFEPVDGPALPAALPGQFLVFRLHTKPGLPPILRNYSLSSAPGSSTYRVSVKQEVNGAGSSFLLNH
VNAGDVLEVSAPRGDFTLLGGDGPVVLVSAGIGATPVLAMLHALASTRSPREVWWLYGARDRKEHPFAKESRELLQSLAR
AQSHIVYSRPQPDDRLGLDYDSPGHLDIPLLDRLGVPQNADFYLCGPPSFLGSFTADLAKRGVDSARIHTEIFGPRESIT
PGIAPAPRPPAHLPAGAPGTGPQVAFARSGITVPWNPTFRSLLELAEACDVPARWSCRTGVCHTCECALIGGTVEYQTLP
LEPPAEGNLLVCCSQPQGDVEIDL
>Mature_583_residues
GRLLSVNVGLPRDITWRGETVHTGIWKHPVSGRQMVRRLNIAGDGQGDLAGHGGEHRAVLVYQSASYRYWESQLNRKNLT
YGQFGENFTVDGLPDDEVFIGDRYRIGGALFEVTQPRVTCYRVGIRMEEPQMAALLVSHHRPGFYLRVLEEGDVGGGDEI
TKVAEGPERITVAAIDALLYLPGHPREQLESALRIPALPPGWKGSLQALLNRGNAESGNPGLTGLAGAPPAWSGFRSLRV
SKVQQECASVFSLCFEPVDGPALPAALPGQFLVFRLHTKPGLPPILRNYSLSSAPGSSTYRVSVKQEVNGAGSSFLLNHV
NAGDVLEVSAPRGDFTLLGGDGPVVLVSAGIGATPVLAMLHALASTRSPREVWWLYGARDRKEHPFAKESRELLQSLARA
QSHIVYSRPQPDDRLGLDYDSPGHLDIPLLDRLGVPQNADFYLCGPPSFLGSFTADLAKRGVDSARIHTEIFGPRESITP
GIAPAPRPPAHLPAGAPGTGPQVAFARSGITVPWNPTFRSLLELAEACDVPARWSCRTGVCHTCECALIGGTVEYQTLPL
EPPAEGNLLVCCSQPQGDVEIDL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=250, Percent_Identity=40, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1787098, Length=304, Percent_Identity=29.2763157894737, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1788104, Length=349, Percent_Identity=25.214899713467, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082350, Length=204, Percent_Identity=27.9411764705882, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1787658, Length=374, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321673, Length=265, Percent_Identity=27.9245283018868, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 62952; Mature: 62821

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLLSVNVGLPRDITWRGETVHTGIWKHPVSGRQMVRRLNIAGDGQGDLAGHGGEHRAV
CCCEEEEECCCCCCEEECCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEE
LVYQSASYRYWESQLNRKNLTYGQFGENFTVDGLPDDEVFIGDRYRIGGALFEVTQPRVT
EEEECCCCHHHHHHCCCCCCCCCCCCCCCEECCCCCCCEEECCEEEECCEEEEECCCCEE
CYRVGIRMEEPQMAALLVSHHRPGFYLRVLEEGDVGGGDEITKVAEGPERITVAAIDALL
EEEEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHCCCCEEEEEEEEEHE
YLPGHPREQLESALRIPALPPGWKGSLQALLNRGNAESGNPGLTGLAGAPPAWSGFRSLR
ECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VSKVQQECASVFSLCFEPVDGPALPAALPGQFLVFRLHTKPGLPPILRNYSLSSAPGSST
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCE
YRVSVKQEVNGAGSSFLLNHVNAGDVLEVSAPRGDFTLLGGDGPVVLVSAGIGATPVLAM
EEEEEEHHCCCCCCEEEEECCCCCCEEEEECCCCCEEEECCCCCEEEEECCCCHHHHHHH
LHALASTRSPREVWWLYGARDRKEHPFAKESRELLQSLARAQSHIVYSRPQPDDRLGLDY
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCC
DSPGHLDIPLLDRLGVPQNADFYLCGPPSFLGSFTADLAKRGVDSARIHTEIFGPRESIT
CCCCCCCCCHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCC
PGIAPAPRPPAHLPAGAPGTGPQVAFARSGITVPWNPTFRSLLELAEACDVPARWSCRTG
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
VCHTCECALIGGTVEYQTLPLEPPAEGNLLVCCSQPQGDVEIDL
CCCEEEEEEECCEEEEEEECCCCCCCCCEEEEECCCCCCEEECC
>Mature Secondary Structure 
GRLLSVNVGLPRDITWRGETVHTGIWKHPVSGRQMVRRLNIAGDGQGDLAGHGGEHRAV
CCEEEEECCCCCCEEECCCEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEE
LVYQSASYRYWESQLNRKNLTYGQFGENFTVDGLPDDEVFIGDRYRIGGALFEVTQPRVT
EEEECCCCHHHHHHCCCCCCCCCCCCCCCEECCCCCCCEEECCEEEECCEEEEECCCCEE
CYRVGIRMEEPQMAALLVSHHRPGFYLRVLEEGDVGGGDEITKVAEGPERITVAAIDALL
EEEEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHCCCCEEEEEEEEEHE
YLPGHPREQLESALRIPALPPGWKGSLQALLNRGNAESGNPGLTGLAGAPPAWSGFRSLR
ECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VSKVQQECASVFSLCFEPVDGPALPAALPGQFLVFRLHTKPGLPPILRNYSLSSAPGSST
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCE
YRVSVKQEVNGAGSSFLLNHVNAGDVLEVSAPRGDFTLLGGDGPVVLVSAGIGATPVLAM
EEEEEEHHCCCCCCEEEEECCCCCCEEEEECCCCCEEEECCCCCEEEEECCCCHHHHHHH
LHALASTRSPREVWWLYGARDRKEHPFAKESRELLQSLARAQSHIVYSRPQPDDRLGLDY
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCEEECCCCCCCCCCCCC
DSPGHLDIPLLDRLGVPQNADFYLCGPPSFLGSFTADLAKRGVDSARIHTEIFGPRESIT
CCCCCCCCCHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHCC
PGIAPAPRPPAHLPAGAPGTGPQVAFARSGITVPWNPTFRSLLELAEACDVPARWSCRTG
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
VCHTCECALIGGTVEYQTLPLEPPAEGNLLVCCSQPQGDVEIDL
CCCEEEEEEECCEEEEEEECCCCCCCCCEEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]