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The map label for this gene is aphA [H]

Identifier: 116622457

GI number: 116622457

Start: 4244455

End: 4245540

Strand: Direct

Name: aphA [H]

Synonym: Acid_3355

Alternate gene names: 116622457

Gene position: 4244455-4245540 (Clockwise)

Preceding gene: 116622452

Following gene: 116622458

Centisome position: 42.59

GC content: 60.41

Gene sequence:

>1086_bases
ATGCGCCAAGCTCCTCCAGATAGCGCCGATCTTTACCGCATGCAAGTGCGTGAAATGCGGAAGTACGCGATGTTCACGCT
CGATCGGGATGGCATTCTAACGTCCTGGAATGCGGGCGTCCAGGACCTGATGGGGTACTCCGAGCAGGAATGGCTCGGCC
GCCACGCCTGCCTGATCTTTTGCCCTGCCGAGAAGGCACAGGAGATTTGCGAGTCCGAAATGCGCAGGGCTCGCGAGGAT
GGCGATGTCACCGATATCCGCTGGCACCGGCACAAGGACGGCAGCGAGTTTTTCGCCAACGGCTTTCTGAGCGCGCTTCG
CGATTCCAATGGCCAGCTCATCGGCTACTCCAAGATCCTGAGCGACGAAACCGCGCGTAAAACGCTGCAGGATTCGCTGA
CGGAATCCAACACCGCTCTCGAGCAATTTGCCTATGCGGCCAGCCATGATCTGCAGGAGCCACTACGCACCATGAGCGCC
TATGCCCAACTGCTAAGCCGCCAGTACAGCGGCAAACTGGATGCCGAAGCGGACCGGATGCTCGCGTTCGTGGTCAGCGC
GTCATCCCGCATGACCAGCATCGTGCGGGACCTGCTGGAGTATGCGCGATTCACCACCGAGGAAGGCCGGCCCGACTCTG
CCTCGATCGATGAGGATCTTGAGGCCGCCCTCTCGCACCTGGATGAAGCGATCAAGGCGAGCGAAGCCATCGTGACGCAC
GATCCCATGCCGATTCTCCAGGTGGATCGCGGACAGATTGTCCGGCTGTTCCAGAACCTGATCGGCAACGCGATCAAGTA
CCGCAAGCCCGATCAGCGTCCCGTAGTGCATGTAACAGCAGAGCTAAAGGGCGCGGAGTGGGTATTCTCGGTAAGCGACA
ACGGCATCGGTTTCGAGCCTTCCGAAGCGTCCAAGATCTTTGAGCCCTTCAAGCGTCTGCACTCCGACACCGAGATTCCC
GGAACCGGTGTGGGGCTGGCGATTTGCCGGCGTATCGTCCAAAGGCAAGGAGGCCGCATCTGGGCGGAGTCGCAGCCGGG
CCAGGGCGCGACTTTTCATTTCACACTCCCGGCAAGCAGCAGTTAG

Upstream 100 bases:

>100_bases
TGGAATCTCGCGAAGGTGGGTTAAGTTACATTTGCGGTTCACACTTTGCGCTTCACCGTCGTGCAGCCCATTAAGATGGG
AGTTGCCCGCCAACCCGCCG

Downstream 100 bases:

>100_bases
GGCTCTGAATGTACTCCACTTGACTAAGATCCCCTAAGGTGGTTTTTATGGATAATAACTGCTTTAGGAGGGAGTAACAA
ATGATACAAGTACGTTTGAT

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MRQAPPDSADLYRMQVREMRKYAMFTLDRDGILTSWNAGVQDLMGYSEQEWLGRHACLIFCPAEKAQEICESEMRRARED
GDVTDIRWHRHKDGSEFFANGFLSALRDSNGQLIGYSKILSDETARKTLQDSLTESNTALEQFAYAASHDLQEPLRTMSA
YAQLLSRQYSGKLDAEADRMLAFVVSASSRMTSIVRDLLEYARFTTEEGRPDSASIDEDLEAALSHLDEAIKASEAIVTH
DPMPILQVDRGQIVRLFQNLIGNAIKYRKPDQRPVVHVTAELKGAEWVFSVSDNGIGFEPSEASKIFEPFKRLHSDTEIP
GTGVGLAICRRIVQRQGGRIWAESQPGQGATFHFTLPASSS

Sequences:

>Translated_361_residues
MRQAPPDSADLYRMQVREMRKYAMFTLDRDGILTSWNAGVQDLMGYSEQEWLGRHACLIFCPAEKAQEICESEMRRARED
GDVTDIRWHRHKDGSEFFANGFLSALRDSNGQLIGYSKILSDETARKTLQDSLTESNTALEQFAYAASHDLQEPLRTMSA
YAQLLSRQYSGKLDAEADRMLAFVVSASSRMTSIVRDLLEYARFTTEEGRPDSASIDEDLEAALSHLDEAIKASEAIVTH
DPMPILQVDRGQIVRLFQNLIGNAIKYRKPDQRPVVHVTAELKGAEWVFSVSDNGIGFEPSEASKIFEPFKRLHSDTEIP
GTGVGLAICRRIVQRQGGRIWAESQPGQGATFHFTLPASSS
>Mature_361_residues
MRQAPPDSADLYRMQVREMRKYAMFTLDRDGILTSWNAGVQDLMGYSEQEWLGRHACLIFCPAEKAQEICESEMRRARED
GDVTDIRWHRHKDGSEFFANGFLSALRDSNGQLIGYSKILSDETARKTLQDSLTESNTALEQFAYAASHDLQEPLRTMSA
YAQLLSRQYSGKLDAEADRMLAFVVSASSRMTSIVRDLLEYARFTTEEGRPDSASIDEDLEAALSHLDEAIKASEAIVTH
DPMPILQVDRGQIVRLFQNLIGNAIKYRKPDQRPVVHVTAELKGAEWVFSVSDNGIGFEPSEASKIFEPFKRLHSDTEIP
GTGVGLAICRRIVQRQGGRIWAESQPGQGATFHFTLPASSS

Specific function: Photoreceptor which exists in two forms that are reversibly interconvertible by light:the R form that absorbs maximally in the red region of the spectrum and the FR form that absorbs maximally in the far-red region [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=27.9527559055118, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788549, Length=342, Percent_Identity=24.2690058479532, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994928, Length=350, Percent_Identity=25.4285714285714, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790436, Length=245, Percent_Identity=27.3469387755102, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=27.1653543307087, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081816, Length=225, Percent_Identity=30.6666666666667, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082128, Length=239, Percent_Identity=26.3598326359833, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=26.2008733624454, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788713, Length=238, Percent_Identity=22.2689075630252, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786600, Length=230, Percent_Identity=25.2173913043478, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003018
- InterPro:   IPR013654
- InterPro:   IPR016132
- InterPro:   IPR001294
- InterPro:   IPR013515
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF01590 GAF; PF02518 HATPase_c; PF00512 HisKA; PF08446 PAS_2; PF00360 Phytochrome [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40471; Mature: 40471

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN ; PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQAPPDSADLYRMQVREMRKYAMFTLDRDGILTSWNAGVQDLMGYSEQEWLGRHACLIF
CCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCHHHHHCCCHHHHHCCCEEEEE
CPAEKAQEICESEMRRAREDGDVTDIRWHRHKDGSEFFANGFLSALRDSNGQLIGYSKIL
ECHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEHHHHH
SDETARKTLQDSLTESNTALEQFAYAASHDLQEPLRTMSAYAQLLSRQYSGKLDAEADRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LAFVVSASSRMTSIVRDLLEYARFTTEEGRPDSASIDEDLEAALSHLDEAIKASEAIVTH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEC
DPMPILQVDRGQIVRLFQNLIGNAIKYRKPDQRPVVHVTAELKGAEWVFSVSDNGIGFEP
CCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCC
SEASKIFEPFKRLHSDTEIPGTGVGLAICRRIVQRQGGRIWAESQPGQGATFHFTLPASS
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCC
S
C
>Mature Secondary Structure
MRQAPPDSADLYRMQVREMRKYAMFTLDRDGILTSWNAGVQDLMGYSEQEWLGRHACLIF
CCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCHHHHHCCCHHHHHCCCEEEEE
CPAEKAQEICESEMRRAREDGDVTDIRWHRHKDGSEFFANGFLSALRDSNGQLIGYSKIL
ECHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEHHHHH
SDETARKTLQDSLTESNTALEQFAYAASHDLQEPLRTMSAYAQLLSRQYSGKLDAEADRM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LAFVVSASSRMTSIVRDLLEYARFTTEEGRPDSASIDEDLEAALSHLDEAIKASEAIVTH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEC
DPMPILQVDRGQIVRLFQNLIGNAIKYRKPDQRPVVHVTAELKGAEWVFSVSDNGIGFEP
CCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCC
SEASKIFEPFKRLHSDTEIPGTGVGLAICRRIVQRQGGRIWAESQPGQGATFHFTLPASS
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]