Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 116621828

GI number: 116621828

Start: 3436527

End: 3438581

Strand: Direct

Name: slt [H]

Synonym: Acid_2710

Alternate gene names: 116621828

Gene position: 3436527-3438581 (Clockwise)

Preceding gene: 116621827

Following gene: 116621835

Centisome position: 34.48

GC content: 66.57

Gene sequence:

>2055_bases
GTGCTGACGCGAGCCTATCGCGAATCGCCGACACCGGCGCGTCGCGCGGCAATTGCCACCCACGTCGCGGCACATCCTGA
GGATGCCCCCCTGGCAAACCTTGCCTTGGGGATCGCGGCCTACGAGCAGAAGAACTGGGCGGCCGCGATCGCGGCCTTGA
GGCCGCTGCCAACCAAGCTGCCGAAGGTCGCCGACTATGCCGCGTATTACCTGGCGGCGGCGCAGGTAGAGAACAACGAG
TTCGCCGGCGTGGGAGCTGAACTGGCTCCGGCGTATCGCAATTCGCCACTGGCGGGGCGGAGCCGGCTGATCGAGGCGCG
ATCGCTGAAAGCCAATGCGCCGGCCGACGCGGTGCGACTGCTGCGCGATCACTATGCCGAACTGCCGCAGCCCGAGGGCG
ATGTTACGCTGGCCGATTGTTACCAGGCGGCGAACGACCTGCCGCATGCGGCCGAGTTCTACCAGCGCGTCTATTACCGG
TACGTAACGGGTGCGGCCGCCACCCGGTCGGCATCCGCGCTGGTGGCGCTCAAGGACCTGATGGGCAGCGCGTACCCGCA
GCCCACCGGTACGCAACTGTTACAGAGGGCCGACCGGTTGCTGGACGCGAAGGAATACGTGGAAGCGCAGCGCGCCTATC
AGTCGGTGGTGGCAGGCGCGGAAGGGCTGGAACGCGATCAGGCGCGGGTGCGCGCGGCCGCGATCGATTACCTGAACGGG
AAGGCGGCTCTGGCGGGGCCGGCGCTGACGGCGCTACAGGTTTCCGACTCGGAGGCGGCGGCGGAACGGTGGTACTACCT
GGCGGAGTGCGCCAGGAAGCGCAACGACGATGCCGAACTGGCGCAGGCGGTGCAGCAGCTGACGGCGCATTACCGGCGAT
CGCCGTGGCGGCTGAAGGCGTTGATGTCGGCGGCGAACCGGTATCTGGTGGTGAACCGGCCGGAAGATTACGTGCCTCTG
TATCGCGCGATCTACGAAGATTTCCCGGAGTCGCCGCAGGCGCCGACCTGCCGCTGGAAGGTGGCGTTCAATGCCTACCA
GCATGATCTGCCAGGGGCCGCGACGCTGCTGCGCGAACATCTACGGGTTTATCCGGCGCACTCGACGGCCGGTGCGGCGC
TGTATTTCCTGGGCCGGCTGATGGAGCGCAACGGCGACAGCGCCGGGGGGCGCGCGTGCTATCAGCGGTTGGCCGCAGTG
TATGAGAATCATTATTACGCCATGCTGGCGCGTGCGCGGCTGAAGAGTGTGCCAGGTTCCGGGGCGGCTTCGGCGGAAGT
CGCGCAATTCGTCGCCGGAATCCGATTTCCCGCGGCGACGGCAATTCCGGCAGAGGCTACCGCGGCTACGGCGCAGCGCA
TCGAGCGGTCGCGTCTGCTGCGCTCGGCGGGGCTCGCGGACCTGGCGGATTCCGAATTGCGATTCGGGTCGCGGACGGAC
GGGCAGCCGGCATTGCTTGCGATCGAATTGGCTTCCGCGGCCGATTCTCCGTACCAGGCAATGCGAGTGATGAAGGGCAT
GAATCCCGAGTACCTGGGAATGCCGGTTCCGGCGGCGCCGCGCAAGTACTGGGAACTGCTGTTCCCGCTGCCGTACCGGA
GCGACCTGGTGCGCAATGCGCAGGAGAGGGAACTGGATCCGTTCCTGCTGGCGGGTCTGATTCGGCAGGAATCGGAGTTC
AACCCGGCAGCGGTATCACATGCGAAGGCGTATGGACTGACGCAGGTGCGTCCGGCGACGGGACGGCTGTTTGCGCGACA
GGCCGGCGTGGCGCGGGTGACGACGAGCGTGTTGTGCCAGCCGGCGGCGAATCTCAAGCTGGGCAGCTCGATTTTGCGTT
CGATGCTGGACAAGCAGGCGGGGAGCGTGGAACAGACGCTGGCGTCGTACAATGCGGGGCCAAACCGGGTGGTGGAATGG
CTGCGGTGGAACACGTACCGGGAGCCGGCGGAGTTCGTGGAGTCGATTCCGTTCACGGAGACCCGGGATTACGTGCAGGC
GGTGTTACGGAACGCGGAGATGTACCGGCGCCTGTATGAGGGCGCGGCAAGATGA

Upstream 100 bases:

>100_bases
GCGCGAACTTTAGCCAATGATGATACGGAACTTCTCGGCAGCTCTTATCCCGGCCCTCTTCTTTTCGCTTAGCCTGTCCG
GCGCTTCGAGCACGCTGGCG

Downstream 100 bases:

>100_bases
ATTGGCCGCGCGATTAGCGGACGCTCCCTAACGGTCGCGGCTTCCATGAGAAGGCCCCCCTCTGTCAAGAAAATAATCTA
CAAGGGCTGAGGGGGTTTCA

Product: lytic transglycosylase catalytic subunit subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MLTRAYRESPTPARRAAIATHVAAHPEDAPLANLALGIAAYEQKNWAAAIAALRPLPTKLPKVADYAAYYLAAAQVENNE
FAGVGAELAPAYRNSPLAGRSRLIEARSLKANAPADAVRLLRDHYAELPQPEGDVTLADCYQAANDLPHAAEFYQRVYYR
YVTGAAATRSASALVALKDLMGSAYPQPTGTQLLQRADRLLDAKEYVEAQRAYQSVVAGAEGLERDQARVRAAAIDYLNG
KAALAGPALTALQVSDSEAAAERWYYLAECARKRNDDAELAQAVQQLTAHYRRSPWRLKALMSAANRYLVVNRPEDYVPL
YRAIYEDFPESPQAPTCRWKVAFNAYQHDLPGAATLLREHLRVYPAHSTAGAALYFLGRLMERNGDSAGGRACYQRLAAV
YENHYYAMLARARLKSVPGSGAASAEVAQFVAGIRFPAATAIPAEATAATAQRIERSRLLRSAGLADLADSELRFGSRTD
GQPALLAIELASAADSPYQAMRVMKGMNPEYLGMPVPAAPRKYWELLFPLPYRSDLVRNAQERELDPFLLAGLIRQESEF
NPAAVSHAKAYGLTQVRPATGRLFARQAGVARVTTSVLCQPAANLKLGSSILRSMLDKQAGSVEQTLASYNAGPNRVVEW
LRWNTYREPAEFVESIPFTETRDYVQAVLRNAEMYRRLYEGAAR

Sequences:

>Translated_684_residues
MLTRAYRESPTPARRAAIATHVAAHPEDAPLANLALGIAAYEQKNWAAAIAALRPLPTKLPKVADYAAYYLAAAQVENNE
FAGVGAELAPAYRNSPLAGRSRLIEARSLKANAPADAVRLLRDHYAELPQPEGDVTLADCYQAANDLPHAAEFYQRVYYR
YVTGAAATRSASALVALKDLMGSAYPQPTGTQLLQRADRLLDAKEYVEAQRAYQSVVAGAEGLERDQARVRAAAIDYLNG
KAALAGPALTALQVSDSEAAAERWYYLAECARKRNDDAELAQAVQQLTAHYRRSPWRLKALMSAANRYLVVNRPEDYVPL
YRAIYEDFPESPQAPTCRWKVAFNAYQHDLPGAATLLREHLRVYPAHSTAGAALYFLGRLMERNGDSAGGRACYQRLAAV
YENHYYAMLARARLKSVPGSGAASAEVAQFVAGIRFPAATAIPAEATAATAQRIERSRLLRSAGLADLADSELRFGSRTD
GQPALLAIELASAADSPYQAMRVMKGMNPEYLGMPVPAAPRKYWELLFPLPYRSDLVRNAQERELDPFLLAGLIRQESEF
NPAAVSHAKAYGLTQVRPATGRLFARQAGVARVTTSVLCQPAANLKLGSSILRSMLDKQAGSVEQTLASYNAGPNRVVEW
LRWNTYREPAEFVESIPFTETRDYVQAVLRNAEMYRRLYEGAAR
>Mature_684_residues
MLTRAYRESPTPARRAAIATHVAAHPEDAPLANLALGIAAYEQKNWAAAIAALRPLPTKLPKVADYAAYYLAAAQVENNE
FAGVGAELAPAYRNSPLAGRSRLIEARSLKANAPADAVRLLRDHYAELPQPEGDVTLADCYQAANDLPHAAEFYQRVYYR
YVTGAAATRSASALVALKDLMGSAYPQPTGTQLLQRADRLLDAKEYVEAQRAYQSVVAGAEGLERDQARVRAAAIDYLNG
KAALAGPALTALQVSDSEAAAERWYYLAECARKRNDDAELAQAVQQLTAHYRRSPWRLKALMSAANRYLVVNRPEDYVPL
YRAIYEDFPESPQAPTCRWKVAFNAYQHDLPGAATLLREHLRVYPAHSTAGAALYFLGRLMERNGDSAGGRACYQRLAAV
YENHYYAMLARARLKSVPGSGAASAEVAQFVAGIRFPAATAIPAEATAATAQRIERSRLLRSAGLADLADSELRFGSRTD
GQPALLAIELASAADSPYQAMRVMKGMNPEYLGMPVPAAPRKYWELLFPLPYRSDLVRNAQERELDPFLLAGLIRQESEF
NPAAVSHAKAYGLTQVRPATGRLFARQAGVARVTTSVLCQPAANLKLGSSILRSMLDKQAGSVEQTLASYNAGPNRVVEW
LRWNTYREPAEFVESIPFTETRDYVQAVLRNAEMYRRLYEGAAR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=193, Percent_Identity=31.6062176165803, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 75045; Mature: 75045

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRAYRESPTPARRAAIATHVAAHPEDAPLANLALGIAAYEQKNWAAAIAALRPLPTKL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHC
PKVADYAAYYLAAAQVENNEFAGVGAELAPAYRNSPLAGRSRLIEARSLKANAPADAVRL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHH
LRDHYAELPQPEGDVTLADCYQAANDLPHAAEFYQRVYYRYVTGAAATRSASALVALKDL
HHHHHHHCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGSAYPQPTGTQLLQRADRLLDAKEYVEAQRAYQSVVAGAEGLERDQARVRAAAIDYLNG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
KAALAGPALTALQVSDSEAAAERWYYLAECARKRNDDAELAQAVQQLTAHYRRSPWRLKA
CCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHH
LMSAANRYLVVNRPEDYVPLYRAIYEDFPESPQAPTCRWKVAFNAYQHDLPGAATLLREH
HHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHCCCHHHHHHHHH
LRVYPAHSTAGAALYFLGRLMERNGDSAGGRACYQRLAAVYENHYYAMLARARLKSVPGS
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAASAEVAQFVAGIRFPAATAIPAEATAATAQRIERSRLLRSAGLADLADSELRFGSRTD
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCCC
GQPALLAIELASAADSPYQAMRVMKGMNPEYLGMPVPAAPRKYWELLFPLPYRSDLVRNA
CCCCEEEEEEHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHCCH
QERELDPFLLAGLIRQESEFNPAAVSHAKAYGLTQVRPATGRLFARQAGVARVTTSVLCQ
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHC
PAANLKLGSSILRSMLDKQAGSVEQTLASYNAGPNRVVEWLRWNTYREPAEFVESIPFTE
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCC
TRDYVQAVLRNAEMYRRLYEGAAR
HHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTRAYRESPTPARRAAIATHVAAHPEDAPLANLALGIAAYEQKNWAAAIAALRPLPTKL
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHC
PKVADYAAYYLAAAQVENNEFAGVGAELAPAYRNSPLAGRSRLIEARSLKANAPADAVRL
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHH
LRDHYAELPQPEGDVTLADCYQAANDLPHAAEFYQRVYYRYVTGAAATRSASALVALKDL
HHHHHHHCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MGSAYPQPTGTQLLQRADRLLDAKEYVEAQRAYQSVVAGAEGLERDQARVRAAAIDYLNG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
KAALAGPALTALQVSDSEAAAERWYYLAECARKRNDDAELAQAVQQLTAHYRRSPWRLKA
CCHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHH
LMSAANRYLVVNRPEDYVPLYRAIYEDFPESPQAPTCRWKVAFNAYQHDLPGAATLLREH
HHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHCCCHHHHHHHHH
LRVYPAHSTAGAALYFLGRLMERNGDSAGGRACYQRLAAVYENHYYAMLARARLKSVPGS
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAASAEVAQFVAGIRFPAATAIPAEATAATAQRIERSRLLRSAGLADLADSELRFGSRTD
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCCC
GQPALLAIELASAADSPYQAMRVMKGMNPEYLGMPVPAAPRKYWELLFPLPYRSDLVRNA
CCCCEEEEEEHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHCCH
QERELDPFLLAGLIRQESEFNPAAVSHAKAYGLTQVRPATGRLFARQAGVARVTTSVLCQ
HHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHC
PAANLKLGSSILRSMLDKQAGSVEQTLASYNAGPNRVVEWLRWNTYREPAEFVESIPFTE
CCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCC
TRDYVQAVLRNAEMYRRLYEGAAR
HHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]