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The map label for this gene is gudD [H]

Identifier: 116621785

GI number: 116621785

Start: 3380696

End: 3381940

Strand: Reverse

Name: gudD [H]

Synonym: Acid_2667

Alternate gene names: 116621785

Gene position: 3381940-3380696 (Counterclockwise)

Preceding gene: 116621793

Following gene: 116621784

Centisome position: 33.94

GC content: 61.29

Gene sequence:

>1245_bases
ATGAAAATCCGGGATATTCGCGCGACCACGGTCACGGTCCCGCTGGAAGCCCCCTTGCGCCATGCAAACGGTTGTCATTG
GGGGCGTTTCGTCCGCACCATCGTCGAAGTCGAGACCGATAATGGCTTGATCGGTCTGGGTGAAATGGGCGGCGGAGGCG
AATCCGCGGAAGCGGTTTTTCGCGCGATGAAGTCATATTTGGTAGGCCACGACCCGGCGCGAATCGAGGAAATGCGGTTC
CTGATCGCCAATCCCACCGCATCACTATATAACAACCGGACCCAAATCTTGGCCGCGTTGGAATTCGCCTGCCTGGACAT
TCTCGGCCAGGCGTGGGGCGTCCCGGTTTGTGAAATTCTCGGCGGCCGCTTGCGCGATCGCGTCCCCTTCGCGGCCTACT
GTTTCTTCCGCTACCCCAATCCCTGCACCGGCGAAGGTGAGGTGCGGACCATCGATCAAGTAGTGTCCAACGCCAAGATT
CTCAAGCAGCGCTTCGGCTTCACCAGCCACAAAATGAAGGCCGGCGTTTTCCCGCAGGATTACGAGCTGGAGGCCTACCG
CGCGCTCGCCTCCACCTTTCCCGGCGACTCCTACCGCTTCGACCCCAACGCCGTCTGGTCCACCGAACAGGCGATCCGCT
TCGGCCAGGCCATCGAAGGCCTGCGCAACGATTACCTGGAAGATCCGGTCTACGGCCTGAACGGCATGCGGCGCACGCGC
GAGAAGGTCCGCGTGCCCCTGGCCACGAATACGGTAGTGGTGAACTTCGAGCAACTCGCGGCCAACGCGCTCGATACCGC
GGTAGATGTAATCCTGCTTGACACGACGTTCTGGGGCGGCATCCGCGCCTGTGTGAAGGCCGCCGCGGTCTGTGAAACTT
TCCAGCTCGGCGTCGCCGTGCATTCCTCAGGCGAACTCGGAATACAACTCGCGAGCATGCTGCATCTCGGTGCGGTCCTA
CCCAACCTCTCCTTCGCCGCCGACGCGCACTACCATCACCTGACCGACGACATCATCCAGGGCGGCCTTATGAAGTACGA
GGAGGGCGCCATTCGCGTACCGGATAAACCGGGCCTTGGCGTAACCCTGAGTCGCGATAAACTGCGCGAATACAGCGAAC
TCTATCAACGCCTCGGCGGCTATCCGTACGATCAGGATCCGGGCCGCCCGGGCTGGACACCCATCGTTCCCAACGACCGG
TGGGCAGACCCCAAAGACGATCGTCCCGTGCAGATTGAGTATTGA

Upstream 100 bases:

>100_bases
GCGCGCGGCGCGTTTAAACGTTTCCCATTGATCGGGCAGCATAAGGTTCCTTTCCGTTTCAAAGGCTCTAACTGAATATT
GTTGTACCATGAAACAAATT

Downstream 100 bases:

>100_bases
TATAGCGTACTGACCATGTCGTTCAAATTCCTCGCACTTTTGCTCACGATCCCCGCCGTCCACGCGGAGACCGGCTATGA
TGCCTGGCTGCGATACGCGC

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKIRDIRATTVTVPLEAPLRHANGCHWGRFVRTIVEVETDNGLIGLGEMGGGGESAEAVFRAMKSYLVGHDPARIEEMRF
LIANPTASLYNNRTQILAALEFACLDILGQAWGVPVCEILGGRLRDRVPFAAYCFFRYPNPCTGEGEVRTIDQVVSNAKI
LKQRFGFTSHKMKAGVFPQDYELEAYRALASTFPGDSYRFDPNAVWSTEQAIRFGQAIEGLRNDYLEDPVYGLNGMRRTR
EKVRVPLATNTVVVNFEQLAANALDTAVDVILLDTTFWGGIRACVKAAAVCETFQLGVAVHSSGELGIQLASMLHLGAVL
PNLSFAADAHYHHLTDDIIQGGLMKYEEGAIRVPDKPGLGVTLSRDKLREYSELYQRLGGYPYDQDPGRPGWTPIVPNDR
WADPKDDRPVQIEY

Sequences:

>Translated_414_residues
MKIRDIRATTVTVPLEAPLRHANGCHWGRFVRTIVEVETDNGLIGLGEMGGGGESAEAVFRAMKSYLVGHDPARIEEMRF
LIANPTASLYNNRTQILAALEFACLDILGQAWGVPVCEILGGRLRDRVPFAAYCFFRYPNPCTGEGEVRTIDQVVSNAKI
LKQRFGFTSHKMKAGVFPQDYELEAYRALASTFPGDSYRFDPNAVWSTEQAIRFGQAIEGLRNDYLEDPVYGLNGMRRTR
EKVRVPLATNTVVVNFEQLAANALDTAVDVILLDTTFWGGIRACVKAAAVCETFQLGVAVHSSGELGIQLASMLHLGAVL
PNLSFAADAHYHHLTDDIIQGGLMKYEEGAIRVPDKPGLGVTLSRDKLREYSELYQRLGGYPYDQDPGRPGWTPIVPNDR
WADPKDDRPVQIEY
>Mature_414_residues
MKIRDIRATTVTVPLEAPLRHANGCHWGRFVRTIVEVETDNGLIGLGEMGGGGESAEAVFRAMKSYLVGHDPARIEEMRF
LIANPTASLYNNRTQILAALEFACLDILGQAWGVPVCEILGGRLRDRVPFAAYCFFRYPNPCTGEGEVRTIDQVVSNAKI
LKQRFGFTSHKMKAGVFPQDYELEAYRALASTFPGDSYRFDPNAVWSTEQAIRFGQAIEGLRNDYLEDPVYGLNGMRRTR
EKVRVPLATNTVVVNFEQLAANALDTAVDVILLDTTFWGGIRACVKAAAVCETFQLGVAVHSSGELGIQLASMLHLGAVL
PNLSFAADAHYHHLTDDIIQGGLMKYEEGAIRVPDKPGLGVTLSRDKLREYSELYQRLGGYPYDQDPGRPGWTPIVPNDR
WADPKDDRPVQIEY

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=425, Percent_Identity=30.8235294117647, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789151, Length=418, Percent_Identity=30.622009569378, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI48994953, Length=363, Percent_Identity=23.9669421487603, Blast_Score=75, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 45911; Mature: 45911

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRDIRATTVTVPLEAPLRHANGCHWGRFVRTIVEVETDNGLIGLGEMGGGGESAEAVF
CCCCEEEEEEEEEECCCCHHHCCCCHHHHHHHHHHEEECCCCEEEECCCCCCCCHHHHHH
RAMKSYLVGHDPARIEEMRFLIANPTASLYNNRTQILAALEFACLDILGQAWGVPVCEIL
HHHHHHHCCCCHHHHHHHHHEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GGRLRDRVPFAAYCFFRYPNPCTGEGEVRTIDQVVSNAKILKQRFGFTSHKMKAGVFPQD
CCHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
YELEAYRALASTFPGDSYRFDPNAVWSTEQAIRFGQAIEGLRNDYLEDPVYGLNGMRRTR
CCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
EKVRVPLATNTVVVNFEQLAANALDTAVDVILLDTTFWGGIRACVKAAAVCETFQLGVAV
HHHCCEEECCEEEEEHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEE
HSSGELGIQLASMLHLGAVLPNLSFAADAHYHHLTDDIIQGGLMKYEEGAIRVPDKPGLG
ECCCCHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCCCEEECCCCEECCCCCCCC
VTLSRDKLREYSELYQRLGGYPYDQDPGRPGWTPIVPNDRWADPKDDRPVQIEY
EEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MKIRDIRATTVTVPLEAPLRHANGCHWGRFVRTIVEVETDNGLIGLGEMGGGGESAEAVF
CCCCEEEEEEEEEECCCCHHHCCCCHHHHHHHHHHEEECCCCEEEECCCCCCCCHHHHHH
RAMKSYLVGHDPARIEEMRFLIANPTASLYNNRTQILAALEFACLDILGQAWGVPVCEIL
HHHHHHHCCCCHHHHHHHHHEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GGRLRDRVPFAAYCFFRYPNPCTGEGEVRTIDQVVSNAKILKQRFGFTSHKMKAGVFPQD
CCHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCC
YELEAYRALASTFPGDSYRFDPNAVWSTEQAIRFGQAIEGLRNDYLEDPVYGLNGMRRTR
CCHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
EKVRVPLATNTVVVNFEQLAANALDTAVDVILLDTTFWGGIRACVKAAAVCETFQLGVAV
HHHCCEEECCEEEEEHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHCEEE
HSSGELGIQLASMLHLGAVLPNLSFAADAHYHHLTDDIIQGGLMKYEEGAIRVPDKPGLG
ECCCCHHHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCCCEEECCCCEECCCCCCCC
VTLSRDKLREYSELYQRLGGYPYDQDPGRPGWTPIVPNDRWADPKDDRPVQIEY
EEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]