Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 116621687

GI number: 116621687

Start: 3252963

End: 3253661

Strand: Reverse

Name: trmD [H]

Synonym: Acid_2569

Alternate gene names: 116621687

Gene position: 3253661-3252963 (Counterclockwise)

Preceding gene: 116621688

Following gene: 116621685

Centisome position: 32.65

GC content: 64.38

Gene sequence:

>699_bases
GTGATCTTCCATGTGCTGACGATCTTCCCCGAGTTTTTCGAGGGACCGTTCGCCCACGGCGTGGTCAAGCGCGCGAAGGA
TAGCGGCATCCTCGACATCCGCATTCACAATCTCCGCGACTGGACCCACGACCGTCATCGCACGGTGGACGACCGGCCGT
TCGGCGGCGCCGAAGGCATGGTGTTGAAATGCGAGCCTATCTTCTCCGCCGTGGAATCTATCTGGCCGGAGCGCCGGGCG
GACCAGAAGGTGGTGCTGCTGTCCGCGCAAGGGCGGCTGTTCGACCAGCCGATGGCCGACCGGCTCAAGGAAAACGCCGA
AATCCTGCTCATCTGCGGACGCTACGAGGGCGTGGACGAACGCGTGGCCGAGCACCTGGCGGACGAGGAAGTCTGCATCG
GTAATTACGTCCTCAGCGGCGGCGAACTCGCGGCGGCGGTTGTAGTGGACACGGTGGCGCGGCTGCTGCCCGGAGTCCTG
GGCAATGAGGCGTCGTCCGCCTTCGAGTCCTTTCAGGACGCGGGCCATGGCGAAGGCCTGCTGGATTGCCCGCACTGGAC
GCGTCCCGCGGATTTCCGCGGCTGGAAGGCGCCCGAAGTGCTGCTCAACGGCAACCACGAGGAAATACGCAAATTCCGCC
GCAAGATCGCTCGGGAAAAAACCGAGAAGTTCCGGCCGGACCTGCTTCCAGGCGAGTGA

Upstream 100 bases:

>100_bases
TGCTGATTCCCTTCGCCCGCGCCATCTGCGTGGAGATCAACCCTGCCGCGAAGCGCATCACGGTAGAATTACCCGAAGGA
TTAAAGGATCTGAACCGCCC

Downstream 100 bases:

>100_bases
CCGCCGCCCCCCTCAACGAGCCGCGACCGTAAAGTAAGGGAGCGATCACTTTGGATGAGGGCTCGACGCTCCCCTCACGG
TCGCGGCTCGTCGGATGACG

Product: tRNA (Guanine37-N(1)-) methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MIFHVLTIFPEFFEGPFAHGVVKRAKDSGILDIRIHNLRDWTHDRHRTVDDRPFGGAEGMVLKCEPIFSAVESIWPERRA
DQKVVLLSAQGRLFDQPMADRLKENAEILLICGRYEGVDERVAEHLADEEVCIGNYVLSGGELAAAVVVDTVARLLPGVL
GNEASSAFESFQDAGHGEGLLDCPHWTRPADFRGWKAPEVLLNGNHEEIRKFRRKIAREKTEKFRPDLLPGE

Sequences:

>Translated_232_residues
MIFHVLTIFPEFFEGPFAHGVVKRAKDSGILDIRIHNLRDWTHDRHRTVDDRPFGGAEGMVLKCEPIFSAVESIWPERRA
DQKVVLLSAQGRLFDQPMADRLKENAEILLICGRYEGVDERVAEHLADEEVCIGNYVLSGGELAAAVVVDTVARLLPGVL
GNEASSAFESFQDAGHGEGLLDCPHWTRPADFRGWKAPEVLLNGNHEEIRKFRRKIAREKTEKFRPDLLPGE
>Mature_232_residues
MIFHVLTIFPEFFEGPFAHGVVKRAKDSGILDIRIHNLRDWTHDRHRTVDDRPFGGAEGMVLKCEPIFSAVESIWPERRA
DQKVVLLSAQGRLFDQPMADRLKENAEILLICGRYEGVDERVAEHLADEEVCIGNYVLSGGELAAAVVVDTVARLLPGVL
GNEASSAFESFQDAGHGEGLLDCPHWTRPADFRGWKAPEVLLNGNHEEIRKFRRKIAREKTEKFRPDLLPGE

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=229, Percent_Identity=48.0349344978166, Blast_Score=214, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 26120; Mature: 26120

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFHVLTIFPEFFEGPFAHGVVKRAKDSGILDIRIHNLRDWTHDRHRTVDDRPFGGAEGM
CEEHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCHHHCCHHHCCCCCCCCCCCCCE
VLKCEPIFSAVESIWPERRADQKVVLLSAQGRLFDQPMADRLKENAEILLICGRYEGVDE
EEECHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCHHH
RVAEHLADEEVCIGNYVLSGGELAAAVVVDTVARLLPGVLGNEASSAFESFQDAGHGEGL
HHHHHHCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
LDCPHWTRPADFRGWKAPEVLLNGNHEEIRKFRRKIAREKTEKFRPDLLPGE
EECCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIFHVLTIFPEFFEGPFAHGVVKRAKDSGILDIRIHNLRDWTHDRHRTVDDRPFGGAEGM
CEEHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEECCHHHCCHHHCCCCCCCCCCCCCE
VLKCEPIFSAVESIWPERRADQKVVLLSAQGRLFDQPMADRLKENAEILLICGRYEGVDE
EEECHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEECCCCCHHH
RVAEHLADEEVCIGNYVLSGGELAAAVVVDTVARLLPGVLGNEASSAFESFQDAGHGEGL
HHHHHHCCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
LDCPHWTRPADFRGWKAPEVLLNGNHEEIRKFRRKIAREKTEKFRPDLLPGE
EECCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA