Click here to switch to the map view.

The map label for this gene is rnhB

Identifier: 116621683

GI number: 116621683

Start: 3246853

End: 3247539

Strand: Reverse

Name: rnhB

Synonym: Acid_2565

Alternate gene names: 116621683

Gene position: 3247539-3246853 (Counterclockwise)

Preceding gene: 116621684

Following gene: 116621682

Centisome position: 32.59

GC content: 64.48

Gene sequence:

>687_bases
ATGCGGCCGATTCCGGCGGGTGGCAAATTCCGTTGTGAAGCTACTTTGGAGCGTGAACTGCGCGCGCGCGGCTTCCGCGC
CGTTGCCGGCGTCGATGAAGTCGGACGCGGCGCGCTGTTCGGCGCTGTATTTGCGGGCGCAGTGATCCTTTCCGAGGAAC
GTCCCATTCGCGGCCTCAACGATAGCAAGCAGCTTGACCCGGAACGCCGGGAAGTTCTCGCGGGCCGCATCCGGGAGCGC
GCTGTCGCTTGGTCGATTGCCGCGGTGGATGCGTCCACTATCGACAGCATCAACATCTACCAGGCATCGCGCATGGCCAT
GCGGATCGCCGTCAGCCGCCTCTCTCCCGCGCCCGATTTCCTGCTGGTGGATGCCGTCCCGCTGGAATTCGCGGTACCGC
AGCGCGCTCTTATCAAAGGTGACGAGCGCTGCCATGCCATCGCCGCCGCCTCCATTCTGGCCAAAGTCGCGCGCGACGAA
TGCATGCGGGTCTGGGACAAGGTCTTCCCCGAATACGGGCTGGCCTCGCACAAAGGCTACTCCACGCCTGAGCACTATCG
CGCCATCGAACAGTACGGCCCTACGCCCCTGCACCGGCTGAGTTTCGAACCGGTTCGCGCCCATTCGCGTTTTCCCCTGG
ATCACGACCGGCAACTCGACCTCTTCGATACGGCGGGCGCCGCATGA

Upstream 100 bases:

>100_bases
GTCCGCACCGGCCGTGTCCGCCGCGCCAAACTCTATTACCTGCGCGCGCTCAAGGGCAAGGCTGCCCGTCTGAAAGAGCG
CGAATAGTTCCGGTCACGCC

Downstream 100 bases:

>100_bases
TGCCTCCCGACGCCCAACCGCCGGCGACAGCCGAAGCGCTCAACCGGCCGTTGCCCGTACCGCCGCCGGTGTATCACTGG
TACCACAAGATGTCGGCGGT

Product: RNase HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MRPIPAGGKFRCEATLERELRARGFRAVAGVDEVGRGALFGAVFAGAVILSEERPIRGLNDSKQLDPERREVLAGRIRER
AVAWSIAAVDASTIDSINIYQASRMAMRIAVSRLSPAPDFLLVDAVPLEFAVPQRALIKGDERCHAIAAASILAKVARDE
CMRVWDKVFPEYGLASHKGYSTPEHYRAIEQYGPTPLHRLSFEPVRAHSRFPLDHDRQLDLFDTAGAA

Sequences:

>Translated_228_residues
MRPIPAGGKFRCEATLERELRARGFRAVAGVDEVGRGALFGAVFAGAVILSEERPIRGLNDSKQLDPERREVLAGRIRER
AVAWSIAAVDASTIDSINIYQASRMAMRIAVSRLSPAPDFLLVDAVPLEFAVPQRALIKGDERCHAIAAASILAKVARDE
CMRVWDKVFPEYGLASHKGYSTPEHYRAIEQYGPTPLHRLSFEPVRAHSRFPLDHDRQLDLFDTAGAA
>Mature_228_residues
MRPIPAGGKFRCEATLERELRARGFRAVAGVDEVGRGALFGAVFAGAVILSEERPIRGLNDSKQLDPERREVLAGRIRER
AVAWSIAAVDASTIDSINIYQASRMAMRIAVSRLSPAPDFLLVDAVPLEFAVPQRALIKGDERCHAIAAASILAKVARDE
CMRVWDKVFPEYGLASHKGYSTPEHYRAIEQYGPTPLHRLSFEPVRAHSRFPLDHDRQLDLFDTAGAA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=181, Percent_Identity=49.7237569060773, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_SOLUE (Q024M2)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_823839.1
- ProteinModelPortal:   Q024M2
- SMR:   Q024M2
- STRING:   Q024M2
- GeneID:   4429235
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_2565
- NMPDR:   fig|234267.9.peg.2451
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- PhylomeDB:   Q024M2
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 25188; Mature: 25188

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPIPAGGKFRCEATLERELRARGFRAVAGVDEVGRGALFGAVFAGAVILSEERPIRGLN
CCCCCCCCCEEEHHHHHHHHHHCCCHHHCCHHHHCCHHHHHHHHHHHHEECCCCCCCCCC
DSKQLDPERREVLAGRIRERAVAWSIAAVDASTIDSINIYQASRMAMRIAVSRLSPAPDF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCE
LLVDAVPLEFAVPQRALIKGDERCHAIAAASILAKVARDECMRVWDKVFPEYGLASHKGY
EEEECCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
STPEHYRAIEQYGPTPLHRLSFEPVRAHSRFPLDHDRQLDLFDTAGAA
CCHHHHHHHHHCCCCCHHHCCCCHHHHCCCCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure
MRPIPAGGKFRCEATLERELRARGFRAVAGVDEVGRGALFGAVFAGAVILSEERPIRGLN
CCCCCCCCCEEEHHHHHHHHHHCCCHHHCCHHHHCCHHHHHHHHHHHHEECCCCCCCCCC
DSKQLDPERREVLAGRIRERAVAWSIAAVDASTIDSINIYQASRMAMRIAVSRLSPAPDF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHCCCCCCE
LLVDAVPLEFAVPQRALIKGDERCHAIAAASILAKVARDECMRVWDKVFPEYGLASHKGY
EEEECCCHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
STPEHYRAIEQYGPTPLHRLSFEPVRAHSRFPLDHDRQLDLFDTAGAA
CCHHHHHHHHHCCCCCHHHCCCCHHHHCCCCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA